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Showing 7 results for Barati

Farid Barati, Ahmad Ali Papahn, Mahsa Afrough, Mohammad Barati,
Volume 9, Issue 1 (7-2011)
Abstract

Background: Control of the medium osmolarity and temperature during long or short time sperm manipulation is essential.
Objective: The objectives of the present study were to find the effects of different osmolarities of modified Tyrodechr('39')s solution and milk on the bull sperm during incubation at above zero temperatures.
Materials and Methods: Semen samples were collected twice from five Najdi bull. Centrifuged and most parts of seminal plasma were removed. First experiment: The concentrated semen were splited into nine aliquots to incubate in three different osmolarities (200, 300 and 400 mOsm) at three temperatures (5, 25 and 39°C) for 15 and 60 min of incubation. Second experiment: The semen samples were splited, mixed with the same volume of whole cow milk (5 and 25°C) and milk with 7% glycerol (5°C) and incubated for 15 and 60 min.
Results: Sperm motility severely affected (p<0.05) by incubation at low ionic tension (200 mOsm/l) especially at low temperature (5°C). The impact of low osmolarity on sperm viability can reduce by increasing the incubation temperature to 39°C. The decreased sperm motility, which was induced by lowering osmolarity, was not improved (p>0.05) by increasing temperature during 1 h of incubation. Milk can protect the sperm viability and motility at cool conditions and there is no beneficial effect of glycerol in combination of milk on sperm incubation at above zero temperatures (p<0.05).
Conclusion: Iso- and hyper-osmotic solutions protect bull sperm motility and viability at 25 and 39°C, while milk can be used for protecting sperm at 5°C. 
Sara Masihi, Mojgan Barati, Javad Marfu, Zahra Eskandari,
Volume 10, Issue 6 (4-2012)
Abstract

Background: Central nervous system malformations are the second most common congenital malformations after congenital heart diseases. These malformations are associated with many instances of morbidity and mortality which underline the importance of prevention and their early diagnosis. Objective: The objective of this study is the diagnosis of neural tube defect (NTDs) in the first trimester and its comparison to second trimester diagnoses in order to reduce the complications associated with late pregnancy terminations and its costs.
Materials and Methods: This study was a trans-sectional study. A total number of 1074 patients who referred to Imam Khomeini Hospital were enrolled in this study. During the 11th-13th (+6 days) gestational week the patients were screened sonographically; subsequently they were re-scanned for fetal anomalies during 18th-20th gestational week, and we followed the babies after birth. Considering that Intracranial Translucency (IT) was introduced in the newer researches, it was, too, measured in 125 of the cases.
Results: In a total number of 1074 patients we had one patient with an anencephalous fetus whom was diagnosed in the first trimester of pregnancy. In the second trimester, we detected one case of myelomenigocele; when we referred to this patient’s first trimester sonography, there was no visible IT. In the 125 cases in whom the IT length was measured, it was normal; the 2nd trimester sonographies in these patients were also normal.
Conclusion: It must be noted that the diagnosis of NTD is more accurate in the second trimester of pregnancy. Consequently it is recommended that in high risk patients, the second trimester sonography be performed transvaginally, and in an earlier gestational age (14th-16th gestational weeks).
Seyed Kamal-Aldin Hoseinzadeh-Sani, Farid Barati, Mahmoud Khaksary Mahabady,
Volume 11, Issue 9 (12-2013)
Abstract

Background: There are many studies focused on long or short storage time of epididymal sperm (EPS) of different species. There are limited studies on preservation or cryopreservation of the domestic goat EPS.
Objective: The aim of the present study was to evaluate the effects of ex vivo cold-storage on freezing of EPS from goat (Capra hircus).
Materials and Methods: In a split-plot design the caprine testes-epididymides (40 pairs) were divided to 4 storage-time groups equally (0, 24, 48 and 72 h), then subjected to cryopreservation using Bioxell. Sperm parameters were analyzed before and after freezing.
Results: Duration of cold-storage as well as freezing at all storage-time points reduced sperm viability and progressive motility while increased sperm tail abnormalities (p<0.0001). Freezing reduced the percentage of cytoplasmic droplets (p<0.0001). The percentage of detached heads was increased at all storage-time points following freezing (p=0.0019), except at time 0 h.
Conclusion: It can be concluded that cold storage [in refrigerator (4oC) for 72 h] of epididymides efficiently protected the goat EPS in terms of progressive motility and viability. However, cold-storage may not protect the goat EPS against cryopreservation with Bioxell.
Sara Masihi, Mojgan Barati, Razieh Mohamadjafari, Marzieh Hashemi,
Volume 12, Issue 5 (6-2014)
Abstract

Background: Fetal nasal bone assessment is a non-invasive procedure that helps provide even greater assurance to patients undergoing their first trimester risk assessment for aneuploidies. Absence or presence of this factor is different in some races.
Objective: The study was aimed to evaluate nasal bone in the first trimester of pregnancy in the indigenous population of Khuzestan Province, and to monitor its value in the diagnosis of chromosomal abnormalities.
Materials and Methods: This study was conducted on 2314 pregnant women between 17-43 years old who referred for first trimester screening for chromosomal abnormalities. Gestational age was between 11-13w + 6 days. Nuchal translucency (NT), fetal heart rate (FHR), crown rump length (CRL), and maternal age and maternal blood serum factors (Free βHCG) and pregnancy-associated plasma protein-A (PAPP-A) and nasal bone were assessed. Finally the risk of trisomies was calculated. The statistical tests are based on the relationship between chromosomal abnormality and the presence or absence of the nasal bone.
Results: In 114 cases we could not examine the nasal bone. Also, in 20 cases missed abortion happened without knowing the karyotype. 2173 cases were delivered normal baby, and in seven cases chromosomal abnormalities were diagnosed. Nasal bone was absent in all three cases with trisomy 21 and six of 2173 cases with normal phenotype (0.3%). With use of the Fisher exact test (p=0.0001), a significant correlation was found between the absence of the nasal bone and the risk of chromosomal abnormality.
Conclusion: Inclusion of the nasal bone in first-trimester combined screening for aneuploidies achieves greater detection rate especially in Down syndrome
Zohreh Sadat, Fazlollah Ghofranipour, Seyed Ali Azin, Ali Montazeri, Azita Goshtasebi, Azam Bagheri, Elham Barati,
Volume 16, Issue 1 (January 2018)
Abstract

Background: Designing a valid and reliable questionnaire that allows a fair evaluation of sexual knowledge and attitudes and develop a proper sexual educational program is necessary.
Objective: The present study was designed to develop and psychometric evaluation of the sexual knowledge and attitudes scale for premarital couples.
Materials and Methods: An exploratory mixed method study was conducted in two phases; in the first, in order to develop a questionnaire an item pool was generated on sexual knowledge and attitudes through focus group discussions and individual interviews. In the second phase, the psychometric properties of the questionnaire were examined. For this purpose, face validity, content validity as well as construct validity were conducted. Reliability was assessed by the Cronbach’s alpha coefficient to assess internal consistency and test-retest reliability.
Results: In the first phase an item pool with 88 questions was generated (sexual knowledge 45 items and sexual attitudes 43 items). In the second phase, the number of final items reduced to 33 and 34 items of sexual knowledge and sexual attitudes respectively, through exploratory factor analysis (EFA). Five factors for sexual knowledge and six factors for sexual attitudes identified by EFA. The Cronbach’s alpha coefficient for two sections was 0.84 and 0.81 respectively. The test- retest correlations for sexual knowledge and sexual attitude was 0.74 and 0.82 respectively.
Conclusion: The findings suggest that the Sexual Knowledge and Attitudes Scale for Premarital Couples is a valid and reliable instrument. Further studies are needed to establish stronger psychometric properties for the questionnaire.
Shirin Barati, Mansoureh Movahedin, Hossien Batooli,
Volume 16, Issue 5 (May 2018)
Abstract

Background: Spermatogonial stem cells are the foundation of spermatogenesis and male fertility. So, their maintenance and culture are very important.
Objective: In this study, we assessed protective effects of the Calligonum on in vitro viability and apoptotic and antiapoptotic genes expression of spermatogonial stem cells.
Materials and Methods: After 24 hr of culture, the spermatogonial stem cells were treated with 30 μM dose of H2O2 and then 10 μg/ml the Calligonum extract was added for 3 wks. Viability was assessed by Trypan blue, apoptosis using PI-Annexin and finally Bax, Bcl-2 and P53 genes expression by Real-Time Polymerase chain reaction.
Results: After 3 wk of treatment, viability in the Calligonum extract+H2O2 group was significantly higher than H2O2 group alone (p=0.001). In the Calligonum extract+H2O2 group, apoptosis, as well as expression of apoptotic genes (Bax and P53), was significantly lower than the group treated with H2O2 alone.
Conclusion: The results of this study showed that 30 μM H2O2 increased apoptosis but decreased viability in spermatogonial stem cells. Calligonum has antioxidant properties that can reduce apoptosis, Bax and P53 expression and increase the viability and Bcl-2 expression.
Razieh Mohammad Jafari, Mahvash Zargar, Mojgan Barati, Somayeh Ershadian,
Volume 19, Issue 1 (January 2021)
Abstract

Background: Preterm labor (PTL) is one of the most important factors in neonatal mortality. Some studies have revealed a reverse relationship between cervical length (CL) and PTL, however, further evidence is needed to confirm it.
Objective: To investigate the predictive value of CL in spontaneous and in vitro fertilization (IVF) pregnancies.
Materials and Methods: This prospective cohort study was performed on 154 pregnant women from 16-26 wk of gestation with singleton fetus in spontaneous delivery (n = 77) and IVF pregnancies (n = 77) and followed up until delivery. Women with multiple pregnancy, placenta previa, cerclage, and congenital anomalies were excluded from study. The cut-off determination was done according to the Roc analysis.
Results: The mean CL in term delivery and PTL groups were 37 ± 7 mm and 31 ± 6 mm, respectively (p < 0.001). The frequency of PTL in spontaneous and IVF pregnancies were 7.8% and 23.27%, respectively (p = 0.007). According to the Roc analysis, the best cut-off for normal pregnancy was ≤36 mm with the negative predictive value of 97.9%, the positive predictive value of 11.4%, sensitivity 83.3%, and specificity of 46.5%. While for the IVF group, the cut off was ≤30 mm, with a negative predictive value of 88.4%, positive predictive value of 57.8%, sensitivity of 63.2%, and specificity of 86%.
Conclusion: In this study, IVF had a significant direct correlation with PTL. CL also had a significant indirect relationship with PTL.
 


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