Volume 19, Issue 3 (March 2021)                   IJRM 2021, 19(3): 271-282 | Back to browse issues page


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Khani S, Abdollahi M, Khalaj A, Heidari H, Zohali S. The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study. IJRM. 2021; 19 (3) :271-282
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1777-en.html
1- Neuroscience Research Center, Qom University of Medical Sciences, Qom, Iran.
2- Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
3- Department of Physiology, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
4- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. Department of Physiology and Pharmacology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran. , physiology_86@yahoo.com
5- Student Research Committee, Qom University of Medical Sciences, Qom, Iran.
Abstract:   (376 Views)
Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women.
Objective: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats.
Materials and Methods: This experimental study was carried out on 36 Wistar female rats (3 wk, 60 ± 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of N. sativa seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study.
Results: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p ≤ 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p ≤ 0.001).
Conclusion: The administration of the N. sativa extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that N. sativa seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective.
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Type of Study: Original Article | Subject: Reproductive Physiology

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