Volume 16, Issue 7 (July 2018)                   IJRM 2018, 16(7): 459-462 | Back to browse issues page

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Paul F, Oladele Obajimi G, Olubukunmi Kolade C. Is semen analysis without strict criteria misleading decisions in IVF? A prospective systematic study. IJRM. 2018; 16 (7) :459-462
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1171-en.html
1- Vine Branch Fertility Clinic, Mokola, Ibadan, Nigeria , Sheyipaul06@yahoo.com
2- University of Ibadan, College of Medicine, Department of Obstetrics and Gynaecology and University College Hospital, Ibadan, Nigeria
3- Vine Branch Fertility Clinic, Mokola, Ibadan, Nigeria
Abstract:   (2600 Views)
Background: Sperm morphology has been strongly linked to fertilization. This makes it an important component in semen analysis. They are usually assessed by world health organization (WHO) standard or Kruger strict criteria in in-vitro fertilization (IVF) centers all over the world. Sperm count, motility, and morphology together form the basis by which patients are allocated into IVF or intra-cytoplasmic sperm injection.
Objective: This study aimed to compare fertilization rates in standard IVF from patients with normal sperm count and motility with and without morphological assessment by WHO guideline.
Materials and Methods: In this prospective cohort study, sperm count, motility, and morphology of 504 men candidate for IVF program over a three years period in our center were evaluated in two groups: Group A (case group) included men with normal sperm count and motility but with a poor morphology and group B (control group) included men with normal sperm count, motility and morphology evaluated by WHO criteria. Fertilization rate in both groups were then analyzed after 16-18 hr post insemination.
Results: Fertilization rate was higher in group B (p=0.028). Participants in group B, apart from having a normal sperm count (32.9±7.2) and motility (62.4±8.9), have a strict morphology of ≥30%. Our result has shown that spermatozoa in group B had a higher fertilization rate (71.4%). Though the sperm count (36.4±6.7) and motility (66.3±7.4) in group A were slightly higher (p=0.058 and p=0.060 respectively) than group B, the fertilization rate was lower.
Conclusion: Our study showed that sperm morphology could be a very important consideration before decisions towards allocation of patients into IVF or intra-cytoplasmic sperm injection.
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Type of Study: Short Research Reports |

1. Franken DR, Oehninger S. Semen analysis and sperm function testing. Asian J Androl 2012; 14: 6-13. [DOI:10.1038/aja.2011.58]
2. Liu DY, Baker HW. Evaluation and assessment of semen for IVF/ICSI. Asian J Androl 2002; 4: 281-285.
3. Lewis SE. Is sperm evaluation useful in predicting human fertility? Reproduction 2007; 134: 31-40. [DOI:10.1530/REP-07-0152]
4. Nallella KP, Sharma RK, Aziz N, Agarwal A. Significance of sperm characteristics in the evaluation of male infertility. Fertil Steril 2006; 85: 629-634. [DOI:10.1016/j.fertnstert.2005.08.024]
5. Guzick DS, Overstreet JW, Factor LP, Brazil CK, Nakajima ST, Coutifaris C, et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2001;345:1388-1393 [DOI:10.1056/NEJMoa003005]
6. Tang YG, Tang LX, Wang QL. The reference values for semen parameters of 1213 fertile men in Guangdong Province in China. Asian J Androl 2015; 17: 298-303. [DOI:10.4103/1008-682X.143251]
7. Barratt CLR, Björndahl L, De Jonge CJ, Lamb DJ, Osorio Martini F, McLachlan R et al. The diagnosis of male infertility: an analysis of the evidence to support the development of global WHO guidance-challenges and future research opportunities. Hum Reprod Update 2017; 23: 660-680. [DOI:10.1093/humupd/dmx021]
8. Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO laboratory manual for the examination and processing of human semen. Asian J Androl 2010; 12: 47-58. [DOI:10.1038/aja.2009.14]
9. World Health Organization. WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organisation, 2010.
10. De Vos A, Van De Velde H, Joris H, Verheyen G, Devroey P, Van Steirteghem A. Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil Steril 2003; 79: 42-48. [DOI:10.1016/S0015-0282(02)04571-5]
11. Gardner Allison J, Evans Janice P. Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple sperm. Reprod Fertil Dev 2005; 18, 53-61. [DOI:10.1071/RD05122]
12. Rath D, Töpfer-Petersen E, Michelmann HW, Schwartz P, Ebeling S. Zona pellucida characteristics and sperm-binding patterns of in vivo and in vitro produced porcine oocytes inseminated with differently prepared spermatozoa. Theriogenology 2005; 63: 352-362. [DOI:10.1016/j.theriogenology.2004.09.044]
13. Huszar G, Jakab A, Sakkas D, Ozenci CC, Cayli S, Delpiano E, et al. Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. Reprod Biomed Online 2007; 14: 650-663. [DOI:10.1016/S1472-6483(10)61060-7]
14. Menkveld R, Holleboom CA, Rhemrev JP. Measurement and significance of sperm morphology. Asian J of Androl 2011; 13: 59-68. [DOI:10.1038/aja.2010.67]
15. Check JH, Check ML, Katsoff D. Prognosis for sperm fertilizability: analysis of different variables in men. Arch Andrology 2002; 48: 73-83. [DOI:10.1080/014850102753385233]
16. Larson KL, DeJonge CJ, Barnes AM, Jost LK, Evenson DP. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod 2000; 15: 1717-1722. [DOI:10.1093/humrep/15.8.1717]
17. Kovac JR, Smith RP, Cajipe M, Lamb DJ, Lipshultz LI. Men with a complete absence of normal sperm morphology exhibit high rates of success without assisted reproduction. Asian J Androl 2017; 19: 39-42.
18. Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril 2004; 81: 1289-1295. [DOI:10.1016/j.fertnstert.2003.09.063]

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