Volume 6, Issue 3 (7-2008)                   IJRM 2008, 6(3): 65-0 | Back to browse issues page

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Moein M R, Soleimani M, Tabibnejad N. Reactive oxygen species (ROS) production in seminal fluid correlate with the severity of varicocele in infertile men. IJRM. 2008; 6 (3) :65-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-109-en.html
1- Department of Andrology, Yazd Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , moein1339@yahoo.com
2- Department of Andrology, Yazd Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (1331 Views)
Background: Reactive oxygen species (ROS) are a group of free radicals that in excessive amounts have negative influence on sperm quality and function.
Objective: To study the effect of varicocele and its severity on the level of ROS in infertile men with clinical varicocele.
Materials and Methods: In this controlled prospective study, 42 men with clinically diagnosed left varicocele and 30 fertile men were studied. Patients were asked about history of urogenital infection, using any antioxidant medication and smoking. Grade of varicocele was determined by physical examination. Levels of ROS in seminal plasma were measured in each group by a chemiluminiscence assay. The sperm parameters were also determined and compared in different groups.
Results: The ROS levels were significantly higher in patients with varicocele than normal men (mean: 1575.42 RLU (Radio Luminescence Unit) vs. 53.79 RLU, p=0.005). In total 20 patients had grade I, 20 patients grade II and 2 patients had grade III varicocele. The mean ROS levels were 669.12 RLU, 2406.87 RLU and 2324 RLU in patients with grade I, II and III varicocele respectively (p=0.144). In case group, 15 patients were smoker and 27 were non-smokers. The mean ROS levels in patients with the history of smoking was 1367.71 RLU while in non-smokers it was 897.672 RLU (p=0.729). Conclusion: Our study showed that increased levels of ROS production in the seminal fluid can be an important factor in the etiology of male infertility in patients with varicocele, and this effect is more prominent with higher grade of varicocele.
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Type of Study: Original Article |

References
1. Schmidt L, Munster K, Helm P. Infertility and the seeking of infertility treatment in a representative population. Br J Obst Gynecol 1995; 102: 978-984. [DOI:10.1111/j.1471-0528.1995.tb10905.x]
2. Schlesinger MH, Wilets IF, Nagler HM. Treatment outcome after varicocelectomy: a critical analysis. Urol Clin North Am 1994; 21:517-529.
3. Kursh ED. What is the incidence in a fertile population? Fertil Steril 1987; 48: 510- 511. [DOI:10.1016/S0015-0282(16)59432-1]
4. Hendin B, Kolettis P, Sharma RK, Thomas Jr AJ, Agarwal A. Varicocele is associated with elevated spermatozoal reactive oxygen species production and diminished seminal plasma antioxidant capacity. J Urol 1999; 161: 1831-1834. [DOI:10.1016/S0022-5347(05)68818-0]
5. Steckel J, Dicker AP, Goldstein M. Relationship between varicocele size and response to varicocelectomy. J Urol 1993; 149:769-771. [DOI:10.1016/S0022-5347(17)36203-1]
6. de Lamirande E, Gagnon C. Human sperm hyperactivation and capacitation as part of an oxidative process. Free Radic Biol Med 1993; 14: 157-166. [DOI:10.1016/0891-5849(93)90006-G]
7. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-843. [DOI:10.1016/S0015-0282(02)04948-8]
8. de Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod 1995; 10: 15-21. [DOI:10.1093/humrep/10.suppl_1.15]
9. Aitken RJ, Clarkson JS, Fishel S. Generation of oxygen reactive species, lipid peroxidation, and human sperm function. Biol Reprod 1989; 41: 183-197. [DOI:10.1095/biolreprod41.1.183]
10. Griveau JF, Le Lannou D. Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl 1997; 20: 61-69. [DOI:10.1046/j.1365-2605.1997.00044.x]
11. Koksal IT, Usta M, Orhan I, Abbasoglu S, Kadioglu A. Potential of reactive oxygen species on testicular pathology associated with infertility. Asian J Androl 2003; 5: 95-99.
12. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urology 1996; 48: 835-850. [DOI:10.1016/S0090-4295(96)00313-5]
13. Mazzilli F, Rossi T, Marchesini M, Ronconi C, Dondero F. Superoxide anion in human semen related to seminal parameters and clinical aspects. Fertil Steril 1994; 62: 862-868. [DOI:10.1016/S0015-0282(16)57017-4]
14. Sharma RK, Pasqualotto FF, Nelson DR, Thomas Jr AJ, Agarwal A. The reactive oxygen species- total antioxidant capacity score is a new measure of oxidative stress to predict male infertility. Hum Reprod 1999; 14: 2801-2807. [DOI:10.1093/humrep/14.11.2801]
15. Allamaneni SR, Naughton CK, Sharma RK, Thomas Jr AJ, Agarwal A. Increased seminal reactive oxygen species levels in patients with varicoceles correlate with varicocele grade but not with testis size. Fertil Steril 2004; 82: 1684-1686. [DOI:10.1016/j.fertnstert.2004.04.071]
16. World Health Organization. WHO manual for the standardized investigation, diagnosis and management of the infertile male. Cambridge: Cambridge University Press, 2000.
17. WHO: World Health Organization laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Fourth edition. New York: Cambridge University press: Cambridge, 1999.
18. Kobayashi H, Gil-guzman E, Mahran AM, Sharma RK, Nelson DR, Thomas Jr AJ, et al. Quality control of reactive oxygen species measurement by Luminol dependent chemiluminescence assay. J Androl 2001; 22: 568-574.
19. Padron OF, Brackett NL, Sharma RK, Lynne CM, Thomas Jr AJ , Agarwal A. Seminal reactive oxygen species and sperm motility and morphology in men with spinal cord injury. Fertil Steril 1997; 67: 1115-1120. [DOI:10.1016/S0015-0282(97)81448-3]
20. Takahara M, Ichikawa T, Shiseki Y, Nakamura T, Shimazaki J. Relationship between grade of varicocele and the response to varicocelectomy. Int J Urol 1996; 3:282-285. [DOI:10.1111/j.1442-2042.1996.tb00535.x]
21. Agarwal A, Prabakaran S, Allamaneni SS. Relationship between oxidative stress, varicocele and infertility: a meta- analysis. Reprod Biomed Online 2006; 12: 630-633. [DOI:10.1016/S1472-6483(10)61190-X]
22. Villanueva-Diaz CA, Vega-Hernandez EA, Diaz-Perez MA, Echavar-ria-Sanchez M, Karchmer-Krivitsky S. Sperm dysfunction in subfertile patients with varicocele and marginal semen analysis. Andrologia 1999; 31: 263-267. [DOI:10.1046/j.1439-0272.1999.00253.x]
23. Saleh R, Agarwal A, Sharma RK, Nelson DR, Thomas Jr AJ. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril 2002; 78: 491-499. [DOI:10.1016/S0015-0282(02)03294-6]

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