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Behnaz Molaei, Farnaz Mohmmadian, Maryam Eftekhar, Robabeh Hatami, Atefe Tirkan, Mahsa Kiani,
Volume 15, Issue 2 (3-2017)

Background: Chlamydia trachomatis and Neisseria gonorrhoeae are the most prevalent bacterial sexually transmitted diseases (STDs) in women.
Objective: The purpose of this study was to investigate the prevalence ofgGonorrheal and cChlamydial infections, and determination of related risk factors inmarried women complaining about vaginal discharge attending gynecological OPDin Zanjan in 2013-2014.
Materials and Methods: In this descriptive/analytic study, 100 women aged 18-49years with vaginal discharge were evaluated for signs and symptoms of gonococcaland chlamydial infections through interviews. Then cervical discharge samples andblood samples were collected from each subject for the detection of Nisseriagonorrhea and Chlamydia trachomatis by bacterial culture and serological tests,respectively. The data from the questionnaires and experimental tests werestatistically analyzed.
Results: The overall prevalence of Chlamydia trachomatis and Nisseria gonorrhoeae were 16% and 4%, respectively. There was no significant relationship between the history of fertility and childbirth, contraception methods, previous history of vaginal infections, previous history of urinary tract infections, number of coitus per weekly and self-reported symptoms (itching, burning, abdominal pain) with incidence of Nisseria gonorrhoeae and Chlamydia trachomatis.
Conclusion: According to results obtained through laboratory tests, the prevalence of gonococcal and chlamydial infections increased, which makes it necessary to put emphasis on education and further preventive and therapeutic programs.
Mahmoud Nateghi Rostam, Batool Hossein Rashidi, Azam Habibi, Razieh Nazari, Masoumeh Dolati,
Volume 15, Issue 6 (7-2017)

Background: Trichomonas vaginalis (T.vaginalis) and Neisseria gonorrhoeae (N.gonorrhoeae) are two most common non-viral sexually transmitted infections in the world. No data are available regarding the epidemiology of genital infections in women of Qom, central Iran.
Objective: Epidemiological investigation of sexually transmitted infections in genital specimens of women referred to the referral gynecology hospital in Qom, central Iran.
Materials and Methods: Genital swab specimens were collected from women volunteers and used for identification of bacterial and protozoal infections by conventional microbial diagnostics, porA pseudo gene LightCycler® real-time PCR (for N.gonorrhoeae) and ITS-PCR (for T.vaginalis).
Results: Of 420 volunteers, 277 (65.9%) had genital signs/symptoms, including 38.3% malodorous discharge, 37.9% dyspareunia, and 54.8% abdominal pain. Totally, 2 isolates of Streptococcus agalactiae were identified. Five specimens (1.2%) in Thayer-Martin culture and 17 (4.1%) in real-time PCR were identified as N.gonorrhoeae. Fifty-four specimens (12.9%) in wet mount, 64 (15.2%) in Dorset’s culture, and 81 (19.3%) in ITS-PCR showed positive results for T.vaginalis. Five mixed infections of T.vaginalis+ N.gonorrhoeae were found. The risk of T.vaginalis infection was increased in women with low-birth-weight (p=0.00; OR=43.29), history of abortion (p=0.00; OR=91.84), and premature rupture of membranes (PROM) (p=0.00; OR=21.75). The probability of finding nuclear leukocytes (p=0.00; OR=43.34) in vaginal smear was higher in T.vaginalis infection.
Conclusion: The significant prevalence of trichomoniasis and gonorrhea emphasizes the need for accurate diagnosis and effective surveillance to prevent serious reproductive complications in women.

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