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Showing 9 results for Torab

Nezhat Moossavifar, Nayereh Khadem, Alieh Torabizadeh,
Volume 4, Issue 1 (7-2006)

Background: It has been demonstrated that performing a mock embryo transfer (ET) significantly improves the pregnancy rate in IVF cycles. The mock ET could be performed prior to the stimulation cycle or immediately before real ET. The problem of the first procedure is the possibility of variation in the uterine position and or cervico–uterine angle.
Objective: The purpose of this study was to determine the consistency of the type of ET in mock ET prior to the treatment cycle with real ET.
Materials and Methods: A prospective comparison between the technique of mock embryo transfer and real embryo transfer. One hundred and sixty treatment cycles on 141 patients between January 2003 and September 2004 ended to ET and they were analyzed prospectively. In each cycle, patients underwent a mock ET in mid luteal phase prior to treatment cycle (the date when patients referred to have consultation about down regulation regimen).
Results: Of 160 mock ET the Wallace catheter passed easily in 144 (90%) of them. In the remaining 16 cycles (10%) a tenaculum and or a rigid cannula had to be used. From 144 cases of easy mock ET, real ET was easy in 121 (84%) cases while 23 (16%) cases had difficultly during real ET. In the 16 cases of difficult mock ET, there was 8 cases (50%) of difficult real ET and 8 cases (50%) of easy real ET (p=0.001, χ2=10.67). The overall clinical pregnancy rate for both difficult and easy transfer was 35%. The pregnancy rate for easy real ET was  37.2% and for difficult real ET was 25.87%. Despite of the difference, the statistical analysis shows no significance difference (χ2=1.21). The difference between mean age for both difficult and easy real transfer was not significant.
Conclusion: Mock ET before beginning of the treatment cycle is highly consistent with real ET.
Zahra Fardiazar, Simin Atashkhouei, Yousef Yosefzad, Mohamad Goldust, Reza Torab,
Volume 11, Issue 1 (4-2013)

Background: Fetal color Doppler is important for evaluation of hypoxia in intrauterine growth restriction (IUGR) fetus.
Objective: In this study we compare fetal and maternal color Doppler with blood gas analysis to detect fetal acidosis.
Materials and Methods: In this cross-sectional study we evaluated 100 hospitalized patients with IUGR for comparison of color Doppler results with arterial blood gas analysis. Results of Doppler sonography of fetus middle cerebral arteries, umbilical and uterine artery and umbilical artery ABG were studied in these neonates.
Results: Mean maternal age was 28±7 years, mean gestational age was 31.79±2.59 weeks and mean growth restriction was 3±2 weeks. Resistance increasing was observed in right uterine arteries of 37 mothers. It was normal in 60 mothers. Resistance increasing was observed in left uterine arteries of 36 mothers and nuch was seen in four cases. PCO2, PO2, and pH mean were 48.41±9.50 mmHg, 26.00±12.34 mmHg, and 7.28±0.10 in the neonates respectively.
Conclusion: In this study abnormal color Doppler in IUGR fetuses have no significant correlation with umbilical cord blood gas.
Mehdi Nikbakht Dastjerdi, Roshanak AbouTorabi, Bahram Eslami Farsani,
Volume 11, Issue 6 (9-2013)

Background: Endometriosis is a female health disorder that occurs when cells from the lining of the uterus grow in other areas of the body. The cause of endometriosis is unknown.
Objective: The purpose of this study was to investigate TP53 gene codon 72 polymorphism in women with endometriosis and compared it with healthy samples in Isfahan.
Materials and Methods: We undertook a case-control study to examine the possible association of the TP53 gene codon 72 polymorphism with the risk of endometriosis in Isfahan. Ninety whole blood specimens from normal people as controls and ninety endometriosis specimens were analyzed. p53 codon 72 genotypes were identified using allele-specific polymerase chain reaction.
Results: Frequency of genotype Arg/Arg (Arginine/Arginine) in the samples of endometriosis was 28.9% and in healthy samples 42.2%. Frequency of genotype Pro/Pro (Proline/Proline) in the samples of endometriosis was 15.6% and in healthy ones. Frequency of heterozygote's Arg/Pro was 55.6% in endometriosis samples and 54.45% in healthy ones 3.3%. By comparing statistical genotype Pro/Pro with two other genotypes in both groups there was a statistical meaningful difference between control group and endometriosis group. [p=0.009, CI=95%, OR=5.34 (1047-19.29)].
Conclusion: Recent research shows that genotype Pro/Pro codon72 exon4 TP53 gene may be one predisposing genetic factor for endometriosis in Isfahan.
Aalie Torabizadeh, Fatemeh Vahidroodsari, Zakieh Ghorbanpour,
Volume 11, Issue 10 (12-2013)

Background: Ovarian hyperstimulation syndrome (OHSS) is the most serious and potentially life-threatening iatrogenic complication associated with ovarian stimulation during Assisted Reproductive Technology (ART) protocols. OHSS typically is a result of ovarian expression of vascular endothelial growth factor (VEGF) which increases vascular permeability.
Objective: Comparison of albumin and cabergoline in the prevention of OHSS.
Materials and Methods: 95 high risk infertile women for OHSS (more than 20 follicles in both ovaries at day of Human Chorionic Gonadotropin (HCG) injection) were randomly divided into two groups. First group including 48 women received 10 unit intravenous albumin at starting oocyte retrieval, and second group including 47 women received 0.5 mg/day dopamine agonist (Cabergolin) at day of HCG injection till 8 days. The dosage of human Menopausal Gonadotropin (HMG) used, total number of follicles developed, number of oocytes retrieved, serum E2 concentrations during the luteal phase, development of ascites, number of embryos generated, clinical pregnancy rate, results of the in vitro fertilization-embryo transfer (IVF-ET) cycles and incidence and severity of any OHSS were evaluated.
Results: There was evidence of a statistically significant reduction in the incidence of OHSS in the cabergolin group (53.7%) versus albumin group (46.3%) (p=0.04). But there was no significant difference of a reduction in severe OHSS (p=0.62). There was no difference in clinical pregnancy rate too.
Conclusion: Administration of cabergolin can prevent incidence of OHSS and does not appear to effect on its severity. 
Fereshte Torabi, Majid Malekzadeh Shafaroudi, Nourollah Rezaei,
Volume 15, Issue 7 (8-2017)

Background: Cyclophosphamide (CP) has been known as an anticancer drug with several side effects on various organs such as a male reproductive system that can cause infertility.
Objective: To evaluate the possible combined effects of zinc oxide nanoparticles (nZno) and melatonin (Mel) on sperm parameters and histopathological changes of the testis in CP-treated rats.
Materials and Methods: 42 adult male Wistar rats were divided into six groups. GI: control, GII: 60 mg/kg/wk CP, GIII and GIV, 10 mg/kg/wk Mel and 5mg/kg/wk nZno and GV: 5 mg/kg/wk nZno and 10 mg/kg/wk Mel were given 2 hr prior to CP injection, respectively,GVI: 5mg/kg/wk nZno and 10 mg/kg/wk Mel simultaneously. After 8 wk of treatment, rats were sacrificed and testis and epididymis were harvested for further evaluation.
Results: The CP-treated group showed significant decreases in the body, testes and epididymis weights and sperm parameters (sperm count, viability, motility) with an increase abnormal sperms when compared with the control (p<0.001), as well as many histological alterations included decreased diameters of seminiferous tubules and Johnsen’s Testicular Score (with degeneration, desquamation, multi-nucleated giant cell formation), whereas combined treatment (GV), showed more protective effects on CP-induced reproductive system damage compared with groups III or IV (p<0.001).
Conclusion: These results suggest simultaneous administration of Mel and nZno have more effectively protections against CP-induced reproductive damage than Mel or nZno alone.
Ehsan Salarkia, Gholamreza Sepehri, Parvin Torabzadeh, Jalil Abshenas, Arezoo Saberi,
Volume 15, Issue 10 (12-2017)

Background: Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial.
Objective: In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and histological changes of testes in male rats.
Materials and Methods: In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i.p., and IV- IX received co-trimoxazole (30, 60, and 120 mg/kg/daily; i.p.)+folic acid (1 mg/kg/daily; i.p.) for 14 or 28 days. Sperm samples were obtained from each group at the end of 14th and 28th days. Sperm numbers, motility, and viability were evaluated on a hemocytometer. Hematoxylin and Eosin stained testes were done for evaluation ofthe number of Leydig cells, vascularity, spermatids, spermatocytes, and means of seminiferous tubules diameter under light microscopy.
Results: Co-trimoxazole treatment for either 14 or 28 days caused a significant decrease in the percentage of sperm number, motility, and viability (p<0.001) compared to the control group. Also, high doses of co-trimoxazole caused a significant decrease in testes structural abnormalities means of seminiferous tubules diameter, spermatids, and spermatogonia) compared to the vehicle group (p<0.001). Folic acid co-administration with co-trimoxazole partially reversed the decrease in sperm quality and structural abnormalities of high doses of co-trimoxazole (60 and 120 mg/kg/daily) (p<0.001).
Conclusion: The data showed the adverse effects of co-trimoxazole on sperm quality and testes morphology which was protected partially by folic acid co-administration in rats. The underlying mechanism (s) needs further investigations.
Abbas Bakhteyari, Yasaman Zarrin, Parvaneh Nikpour, Zeinab Sadat Hosseiny, Fatemah Sadat Mostafavi, Nahid Eskandari, Mohammad Matinfar, Roshanak AbouTorabi,
Volume 17, Issue 6 (June 2019 2019)

Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition?
Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation.
Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Realtime PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium.
Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression.
Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.
Fatemeh Amin Marashi, Ali Torabi, Francis Beaudry,
Volume 17, Issue 6 (June 2019 2019)

Background: Fibroblast growth factors (FGFs) are growth factors that have diverse biological activities including broad mitogenic and cell survival activities. They function through the activation of a specific tyrosine kinase receptor that transduces the signal
by activating several intracellular signaling pathways.
Objective: To identify the different signaling pathways involved in the mechanism of action of FGF8 and FGF18 on ovine granulosa cells using mass spectrometry.
Materials and Methods: Ovine ovarian granulosa cells were harvested from adult sheep independently at the stage of the estrous cycle and were cultured at a density of 500,000 viable cells in 1 ml DMEM/F12 medium for five days. The cells were then treated on day 5 of culture with 10 ng/mL FGF8 and FGF18 for 30 minutes, and total cell protein was collected for mass spectrometry.
Results: Mass spectrometry showed that both FGF8 and FGF18 significantly induce simultaneous upregulation of several proteins, including ATF1, STAT3, MAPK1, MAPK3, MAPK14, PLCG1, PLCG2, PKCA, PIK3CA, RAF1, GAB1, and BAG2 (> 1.5-fold; p < 0.01).
Conclusion: ATF1 and STAT3 are important transcription factors involved in cell growth, proliferation and survival, and consequently can hamper or rescue the normal ovine reproductive system function.
Farideh Zafari Zangeneh, Maryam Sarmast Shoushtari, Sahar Shojaee, Elahe AbouTorabi,
Volume 18, Issue 3 (March 2020)

Background: Polycystic ovary syndrome (PCOS) is a multifactorial and heterogeneous disease that has a potent inheritable component based on familial clustering. Despite many studies in the genetic field of PCOS, the genes that are involved in the causes of this syndrome have not been thoroughly investigated.
Objective: The purpose of this study was to establish the occurrence of the Trp64Arg polymorphism of beta3 adrenergic receptor in non-obese women with PCOS.
Materials and Methods: This cross-sectional study was performed on 100 women with PCOS and normal women as the control group in Imam Khomeini Hospital of Tehran in 2016-2017. Peripheral blood sample (2 cc) was obtained from two groups for genomic DNA based on the gene bank. Polymorphisms were genotyped by of using ADRB3 Trp64Arg. Then the DNA was extracted by genomic kiagen kit. The primer was analyzed for PCR based on gene bank by using Primer3 software and then confirmed by primer Blast tool at NCBI site to conformity to the beta-3 adrenergic receptor gene. The protein changes were assessment by the Clastal W software.
Results: The sequence analysis presented in NCBI, transcript variant 1, with the code NM_000025.2, shows changes in the amino acid sequence of exon 1 in women with PCOS. Polymorphism in the codon 64 encoding the amino acid tryptophan (W) occurred in the nucleotide c.T190C, which changed the nucleotide T to C and then the amino acid sequence of the tryptophan was altered to arginine pW64R.
Conclusion: T-C polymorphism is evident in the codon 64 of the adrenergic β3 receptor in patients with PCOS. Therefore, Beta3 adrenergic receptor gene polymorphism (Thr164Ile) associates with this syndrome in nonobese women.

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