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Showing 7 results for Tahajjodi

Somayyeh Sadat Tahajjodi, Maryam Amerion, Nasser Mahdavi Shahri, Mehdi Jalali, Mohammad Reza Nikravesh,
Volume 12, Issue 4 (5-2014)
Abstract

Background: Nicotine can pass through placental blood barrier and accumulate in the developing organs of fetus. Also, entering the breast milk, nicotine can have an effect on the neonates. Investigations have showed that collagen IV is one of the most important micro vessels basement membrane components.
Objective:  In this study, the effect of maternal nicotine exposure in pre and postnatal periods on collagen IV in microvessels of neonatal Balb/C mice brain cortex was studied by immunohistochemistry technique.
Materials and Methods:  24 pregnant Balb/C mice were divided in to 4 groups (6 mice in each group): two experimental and 2 control groups. The mothers in the 1st experimental group were injected 3 mg/kg nicotine intrapritoneally from the 5th day of pregnancy to parturition daily and in 2nd experimental group the same procedure was repeated to the 10th day after parturition (lactation). The control groups received the same volume of normal saline during the same time. 10 days after delivery, the brain tissues of newborns were isolated. Then, prepared blocks from fixed brain were cut serially for immunohistochemical assay.
Results:  The findings of the present study indicated that collagen IV reaction in microvessels basement membrane in the first experimental group increased significantly compared to the first control group (p=0.002). In addition, collagen IV reaction in microvessels basement membrane in the 2nd experimental group increased significantly compared to the 2nd control group (p=0.002). However, no significant difference was observed between the two experimental groups.
Conclusion:  These results suggested that maternal nicotine exposure during prenatal period may increase basement membrane collagen IV expression. Also, nicotine increases in maternal breast milk has no effect on basement membrane collagen IV expression.
Fatemeh Akyash, Somayyeh Sadat Tahajjodi, Fatemeh Sadeghian-Nodoushan, Abbas Aflatoonian, Ali-Mohammad Abdoli, Habib Nikukar, Behrouz Aflatoonian,
Volume 14, Issue 9 (9-2016)
Abstract

This paper summarizes the proceedings of a 1 day national symposium entitled “Reproductive biology, stem cells biotechnology and regenerative medicine” held at Shahid Sadoughi University of Medical Sciences, Yazd, Iran on 3rd March 2016. Here, we collected the papers that presented and discussed at this meeting by specialists that currently researched about the overlaps of the fields of reproductive biology and stem cells and their applications in regenerative medicine
Fatemeh Akyash, Fatemeh Sadeghian-Nodoushan, Somayyeh Sadat Tahajjodi, Habib Nikukar, Ehsan Farashahi Yazd, Mostafa Azimzadeh, Harry D Moore, Behrouz Aflatoonian,
Volume 15, Issue 5 (6-2017)
Abstract

This report explains briefly the minutes of a 1-day workshop entitled; “human embryonic stem cells (hESCs) and good manufacturing practice (GMP)” held by Stem Cell Biology Research Center based in Yazd Reproductive Sciences Institute at Shahid Sadoughi University of Medical Sciences, Yazd, Iran on 27th April 2017. In this workshop, in addition to the practical sessions, Prof. Harry D. Moore from Centre for Stem Cell Biology, University of Sheffield, UK presented the challenges and the importance of the biotechnology of clinical-grade human embryonic stem cells from first derivation to robust defined culture for therapeutic applications
Zahra Borzouie, Somayyeh Sadat Tahajjodi, Behrouz Aflatoonian,
Volume 15, Issue 6 (7-2017)
Abstract

This paper summarizes the proceedings of the stem cell session of the “7th Yazd International Congress and Student Award in Reproductive Medicine” held at Shahid Sadoughi University of Medical Sciences, Yazd, Iran, on 28-30 April 2017. Here, we collected the papers of the session entitled: “Stem Cells, Good manufacturing practice, and tissue engineering”, that presented and discussed at this meeting by the international and national experts of the overlaps of the fields of stem cells and reproductive medicine, and the translation of these efforts towards practical application in regenerative medicine.
Fatemeh Akyash, Somayyeh Sadat Tahajjodi, Ehsan Farashahi Yazd, Fatemeh Hajizadeh-Tafti, Fatemeh Sadeghian-Nodoushan, Jalal Golzadeh, Hassan Heidarian Meimandi, Harry Moore, Behrouz Aflatoonian,
Volume 17, Issue 12 (December 2019)
Abstract

Background: Cell banking of initial outgrowths from newly derived human embryonic stem cells (hESCs) requires an efficient freezing method. Vitrification is used for the preservation of gametes and early embryos in assisted reproduction techniques (ART). Moreover, vitrification was applied for cryopreservation of hESCs using open pulled straws.
Objective: To derive and characterize new hESC lines and then use Cryotech and Cryowin tools for their vitrification.
Materials and Methods: Human ESC lines were generated in a microdrop culture system using mouse embryonic fibroblasts (MEFs) as the feeder layer; this was later scaled up using both MEFs and Yazd human foreskin fibroblasts batch 8 (YhFF#8). To bank the cell lines, master cell banks of 100 Cryotech and Cryowin tools were produced for each individual cell line using the vitrification method; flasks of hESC lines were also cryopreserved using a conventional slow-freezing method.
Results: The pluripotency of cell lines was assessed by their expression of pluripotency-associated genes (OCT4/POU5F1, NANOG, and SOX2) and markers such as SSEA4, TRA-1-60, and TRA-2-49. Their in vitro capacity to differentiate into germ layers and germ cells using embryoid body (EB) formation and monolayer culture was assessed by screening the expression of differentiation-associated genes. The chromosomal constitution of each hESC line was assessed by G-banding karyotyping.
Conclusion: Cryotech and Cryowin tools used to vitrify new hESCs at an early stage of derivation is an efficient method of preserving hESCs.
 
 
Somayyeh Sadat Tahajjodi, Ehsan Farashahi Yazd, Azam Agha-Rahimi, Reza Aflatoonian, Mohammad Ali Khalili, Mahnaz Mohammadi, Behrouz Aflatoonian,
Volume 18, Issue 1 (January 2020)
Abstract

Background: Cumulus cells, as oocyte nurse cells, provide a suitable microenvironment with growth factors and cellular interactions required for oocyte maturation. Thus, these cells may serve as a natural niche for in vitro studies of female germ cell development. Cumulus cells may help attain a better understanding of the causes of infertility in women and eventually improve the outcomes of cases that respond poorly to standard infertility treatment.
Objective: The aim of this study was to isolate, culture, and investigate the biological characteristics of human cumulus cells.
Materials and Methods: In this experimental study, cumulus cells were isolated, cultured, and characterized using reverse transcription-polymerase chain reaction analyses of specific genes including FOXL2, CYP19A1, FSHR, AMHR, and LHR. The presence of vimentin, a structural protein, was examined via immunofluorescent staining. Moreover, levels of anti-mullerian hormone (AMH) and progesterone secretion by cumulus cells were measured with ELISA after 2, 4, 12, 24, and 48 hr of culture.
Results: In adherent culture, human cumulus cells expressed specific genes and markers as well as secreted AMH and progesterone into the medium.
Conclusion: Cumulus cells secrete AMH and progesterone in an adherent culture and might be applicable for in vitro maturation (IVM) and in vitro gametogenesis (IVG) studies.
 

Maryam Adib, Seyed Morteza Seifati, Mahmood Dehghani Ashkezari, Arezoo Khoradmehr, Roshan Rezaee-Ranjbar-Sardari, Somayyeh Sadat Tahajjodi, Behrouz Aflatoonian,
Volume 18, Issue 12 (December 2020)
Abstract

Background: To increase the results of infertility treatment, many efforts have been made to improve the treatment methods. As assisted reproductive technology is mainly using cell culture methods, one of the approaches to improve this technology is conditioned medium from different sources. It is desirable to apply in vitro maturation (IVM) and use oocytes from normal cycles instead of stimulating ovulation.
Objective: To investigate the effect of human cumulus cell condition medium (hCCCM) on the IVM of immature mouse oocytes and morphology.
Materials and Methods: In this experimental study, 240 germinal vesile oocytes were collected from four-six wk-old mice after 48 hr of 5IU pregnant mare serum gonadotropin (PMSG) injection and cultured in hCCCM (test group, n = 120) and DMEM + 20% FBS (control group, n = 120). The IVM rates and changes in perivitelline space (PVS) and shape were investigated at 8, 16, and 24 hr following the culture. The mature (MII) oocytes were subjected to in vitro fertilization (IVF) and the fertilization rate was assessed in three days.
Results: A significant difference was observed between the maturation rates in the hCCCM and control groups (24.16% vs 0%; p = 0.001), as well as morphologic changes between the two groups (p = 0.04, p = 0.05). The development rate for MII oocytes attained from IVM in the hCCCM group was 27.58% (2-cell) and 6.89% (4-cell). Data displayed that hCCCM is an effective medium for oocytes maturation compared to the control medium.
Conclusion: hCCCM supports oocyte in vitro growth and maturation. Moreover, hCCCM changes the oocyte shape and size of perivitelline space. 

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