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Showing 8 results for Soleimani Mehranjani

Malek Soleimani Mehranjani, Masood Hemadi,
Volume 5, Issue 4 (7-2007)
Abstract

Background: Sodium arsenite and/ or vasectomy may cause variation in sex hormones which affect pathophysiology of reproductive organs.
Objective: The aim was to investigate the morphological changes in structure of testis and hormonal imbalance in bilateral Vasectomised rats treated with sodium arsenite.
Materials and Methods: Four groups of rats: bilateral vasectomy + sodium arsenite, bilateral vasectomy, sham operated + sodium arsenite and sham operated only were considered, and 8 mg/kg/ day of sodium arsenite was given for 8 weeks to the rats. The total volume of testis, volume of interstitial tissue, volume of seminiferous tubules, diameter of seminiferous tubules and germinal epithelium thickness were evaluated using stereological methods. Hormones were also measured and the results were analyzed using one way ANOVA.
Results: A significant reduction of total volume of testis (p<0.01), mean volume of seminiferous tubules (p<0.002) as well as germinal epithelium thickness (p<0.05) in both vasectomy + sodium arsenite and vasectomy rats was seen compared to sham operated only. In addition a significant reduction of testosterone was observed in vasectomy + sodium arsenite group when compared to the other groups (p<0.001). LH level decreased significantly in vasectomy + sodium arsenite when compared to sham operated ones (p<0.05).
Conclusion: Vasectomy and treatment with sodium arsenite affect the structure of testis with respect to its volume, volume of seminiferous tubules and thickness of germinal epithelium, which may be due to variation of LH and testosterone level in the rats.
Hamid Reza Momeni, Malek Soleimani Mehranjani, Mohammad Hosien Abnosi, Monireh Mahmoodi,
Volume 7, Issue 3 (7-2009)
Abstract

Background: para-nonylphenol (p-NP) is able to induce malformations in male reproductive system.
Objective: The aim of this study was to investigate the preventing role of vitamin E (Vit.E) on sperm parameters and reproductive hormones in developing rats.
Materials and Methods: Pregnant rats were divided into 4 groups: control p-NP Vit.E and p-NP+Vit.E. Treatments were performed on day 7 of gestation and continued during weaning. The male pups were then divided into the same groups as the mothers and were treated till 90 days of age. Finally body and left testis weight were recorded and left epididymis was cut in Ham’s F10. Released sperm were used to analyze number motility and viability of the sperm. Blood serum was used to assess follicle stimulating hormone (FSH) luteinizing hormone (LH) estrogen and testosterone.
Results: In p-NP-treated rats a significant decrease was found in body and testis weight sperm number and sperm motility compared to control and p-NP+Vit.E groups. A significant increase was also found in sperm viability in Vit.E group (83.3±7.6) compared to both p-NP (59.5±7.5) and control (66.3±9.7) groups. Rats treated with p-NP showed a significant decrease in FSH level and a significant increase in estrogen level. However testosterone and LH level remained constant. In p-NP+Vit.E group the change of estrogen level but not FSH was significantly reversed compared to p-NP group. Conclusion: Vit.E not only is able to compensate the toxic effects of p-NP on testis weight sperm number sperm motility and estrogen level but also increases sperm viability in developing rat.
Malek Soleimani Mehranjani, Ali Noorafshan, Ahmad Hamta, Hamid Reza Momeni, Mohammad Hussein Abnosi, Monireh Mahmoodi, Morteza Anvari, Maryam Hazaveh,
Volume 8, Issue 1 (7-2010)
Abstract

Background: Para-Nonylphenol (p-NP) is one of the environmental pollutants which cause reproductive system disorders.
Objective: The effects of vitamin E on ovary structure during its development in rats treated with p-NP.
Materials and Methods: 32 Wistar female rats after mating were divided into 4 groups; control vitamin E (100mg/kg/day) p-NP (250mg/kg/day) and p-NP + vitamin E. The rats were treated from the day 7 of pregnancy till 21st day of postnatal through sucking period. After weaning the female pups were treated by gavages for 120 days. The total volume of ovary number of follicles volume of oocyte follicular cells and their nuclei and the thickness of zona pellucida were estimated stereologically. The results were analyzed using one way ANOVA and p<0.05 was considered significant.
Results: The ovary weight, mean total volume of ovary and cortex, number of antral and graafian follicles and body weight were decreased significantly (p<0.05) in the p-NP treated rats compared to control and other groups, while the number of atretic follicles was increased significantly (p<0.05). A significant reduction (p<0.05) in volume of oocyte, follicular cells and their nuclei in antral and graafian follicles was found in p-NP group. In addition, treatment with only vitamin E showed an improving effect on folliculogenesis due to a highly significant increase (p<0.01) in the number of primordial follicles.
Conclusion: Vitamin E could compensate the adverse effects of p-NP on the ovary structure during its development.
Elham Aliabadi, Malek Soleimani Mehranjani , Zahra Borzoei, Tahereh Talaei-Khozani, Hossein Mirkhani, Hamed Tabesh,
Volume 10, Issue 2 (7-2012)
Abstract

Background: Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality.
Objective: The aim of this investigation was to evaluate the improvement of the testicular sperm motility and chromatin quality exposed to L-carnitine (LC) and L-acetyl-carnitine (LAC), which are normally concentrated in testis and epididymis, compared with Pentoxifylline (PF), which used for sperm motility enhancement in IVF procedures.
Materials and Methods: TESE samples from 30 male mice divided into four parts. The sperm samples were added to Hamchr('39') F10 (control) or the media contained 1.76mM of LC, LAC or PF), then, the samples were kept in the room temperature for 30, 90 and 180 min. At each time step, sperm motility and chromatin quality were assessed. Chromatin quality was evaluated by chromomycin A3 and aniline blue. Statistical analysis was performed using one way analysis of variance (ANOVA). A p-value less than 0.05 were accepted as a statistically significant difference.
Results: The results showed LC, LAC and PF significantly increased the sperm motility. However, sperm chromatin quality only improved significantly by administration of LC and LAC.
Conclusion: Administration of LC and LAC to the testicular sperm samples can lead to improve both sperm motility and chromatin quality. It may be because they can mimic in vivo sperm condition during late spermatogenesis.
Malek Soleimani Mehranjani, Rezvan Taefi,
Volume 10, Issue 6 (4-2012)
Abstract

Background: Vitamin E is an effective antioxidant, protecting cells against oxidative stress.
Objective: In this investigation the protective effect of vitamin E on the testis during development and spermatogenesis in rats exposed to sodium arsenite was evaluated.
Materials and Methods: Pregnant Wistar rats were divided into 4 groups (n=8) control, sodium arsenite (8 mg/kg/day), sodium arsenite+vitamin E (100 mg/kg/day) and vitamin E. Treatment was carried out from day seven of pregnancy till 90 days. Finally the right testis was stereologically studied. The obtained data was analyzed using one way ANOVA and Tukeychr('39')s test and the means difference was considered significant at p<0.05.
Results: The weight and volume of testis, volume of seminiferous tubules and its diameter, volume of interstitial tissue, height of germinal epithelium and the total number of types A and B spermatogonia, spermatocyte, spermatid and sertoli cells reduced significantly in sodium arsenite group compared to the control. Co-administration of vitamin E and sodium arsenite compensated the adverse effects of sodium arsenite on the above parameters.
Conclusion: We concluded co-treatment of rats with sodium arsenite and vitamin E could prevent the adverse effects of sodium arsenite exposure on the testicular tissue during the prenatal stage till sex maturity.
Malek Soleimani Mehranjani, Tayebeh Mansoori,
Volume 14, Issue 6 (6-2016)
Abstract

Background: Bisphenol A (BPA), an environmental pollutant, can generate free radicals which damages the reproductive system. Vitamin C is an antioxidant which may prevent the adverse effects of free radicals.
Objective: The aim was to investigate the effect of vitamin C on the ovary tissue in rats treated with BPA.
Materials and Methods: In this experimental study, 24 female Wistar rats (200±20 gr) were randomly divided into 4 groups (n=6): control, BPA (60 μg/Kg/day), vitamin C (150 mg/Kg/day) and BPA + vitamin C and orally treated for 20 days. The left ovaries were taken out, fixed for tissue processing and studied using stereological methods. Data were analyzed with SPSS using one-way ANOVA, and the means were considered significantly different at (p<0.05).
Results: The total volume of ovary and cortex (p<0.01), medulla (p<0.05), the volume of corpus luteum (p<0.001) and the mean number of antral follicles (p<0.001) significantly reduced in BPA group compared with control, while the number of atretic follicles increased (p<0.05). The volume of oocyte (p<0.01) and its nucleus (p<0.001) in the antral follicles and the thickness of zona pellucida (ZP) in the secondary (p<0.05) and antral (p<0.001) follicles significantly decreased in BPA group compared with controls. The above parameters in the BPA + vitamin C group were compensated to control level.
Conclusion: Vitamin C can be used as a potential antioxidant in the case of BPA toxication.
Zahra Kalhori, Malek Soleimani Mehranjani, Mehri Azadbakht, Mohammad Ali Shariaatzadeh,
Volume 16, Issue 4 (April 2018)
Abstract

Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder featured by insulin resistance and hyperandrogenism. Testosterone enanthate can induce PCOS in mice models.
Objective: We investigated the ovary stereological features along with the oxidative stress and inflammatory factors in mice following PCOS induction using testosterone enanthate.
Materials and Methods: Twelve female NMRI mice (3 wk old) were divided into 2 groups (n=6/each): Control and PCOS. PCOS was induced through daily injections of testosterone enanthate (1 mg/100g subcutaneous s.c for 5 wk). Finally, ovaries were studied stereologically. The serum levels of the follicle-stimulating hormone, luteinizing hormone, testosterone, interleukin-6, and tumor necrosis factor-α were measured using ELISA kit. Serum levels of Malondialdehyde and the antioxidant capacity were measured relatively using thiobarbituric acid and ferric reducing antioxidant power assay.
Results: The mean total volume of ovary and the mean volume of cortex (p<0.001), volume of oocyte in the preantral (p=0.011) and antral follicle (p=0.015), thickness of zona pellucida (p=0.016), the number of antral follicles (p=0.012), the serum levels of follicle-stimulating hormone (p<0.001) and the antioxidant capacity (p=0.020) reduced significantly in the PCOS group compared to the control. The number of primary (p=0.017) and preantral (p=0.006) follicles and the serum levels of testosterone (p<0.001), Luteinizing hormone (p=0.002), Malondialdehyde, Interleukin 6 and Tumor necrosis factor-α (p<0.001) showed a significant increase in the PCOS group compared to the control.
Conclusion: Testosterone enanthate induced PCOS causes stereological features in the ovary, increases the oxidative stress and inflammatory markers in mice.
Parisa Azizi, Malek Soleimani Mehranjani,
Volume 17, Issue 10 (October 2019)
Abstract

Abstract

Background: Para-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals.

Objective: The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats.

Materials and Methods: 24 adult male Wistar rats (215 ± 20 gr) were randomly divided into four groups (n = 6/each) – including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE – and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated.

Results: A significant decrease in the sperm number, motility, viability and morphology (p < 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p < 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p < 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE.

Conclusion: It seems GTE can be used as a potent antioxidant in the case of p-NP toxication.

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