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Showing 28 results for Soleimani

Alireza Vahidi, Seyed Mehdi Kalantar, Mehrdad Soleimani, Mohammad Hossein Amir Arjmand, Abbas Aflatoonian, Mohammad Ali Karimzadeh, Afsaneh Kermaninejhad,
Volume 2, Issue 2 (7-2004)

Backgrownd: Studies in regions with seasonal climatic variations have revealed a correlation between human natural conception and birth rates. Holidays and other cultural activities probably have influence on conception, but the ambient temperature and emotional influences on the female hormones related to fertility may play an important part in the seasonal variation in conception. Objectives: The aim of study was to determine the relationship between the success rate of Assisted Reproductive Technique (ART) treatment cycles and temperature in different seasons. Materials and Methods: A retrospective study on all individuals undergoing assisted ART at our institution was performed during June 2000 to June 2001. The study population represented 258 IVF-ET cycles and 821 ICSI treatment cycles. Different variables were analyzed using ?2 test. Results: In IVF treatment cycles, conception was more common from early spring (March to June). This decreased from spring, with the minimum in fall, 22% and 14%, respectably. A significant seasonal variability in the number of eggs, embryo transferred and sperm motility was not demonstrated (p>0.05), but sperm count was significantly higher in spring than any other season (72+4 x106 and 52+7x106, respectively). Conclusion: The seasonal changes should be taken into account together with other factors when evaluating infertility data. Article
Malek Soleimani Mehranjani, Masood Hemadi,
Volume 5, Issue 4 (7-2007)

Background: Sodium arsenite and/ or vasectomy may cause variation in sex hormones which affect pathophysiology of reproductive organs.
Objective: The aim was to investigate the morphological changes in structure of testis and hormonal imbalance in bilateral Vasectomised rats treated with sodium arsenite.
Materials and Methods: Four groups of rats: bilateral vasectomy + sodium arsenite, bilateral vasectomy, sham operated + sodium arsenite and sham operated only were considered, and 8 mg/kg/ day of sodium arsenite was given for 8 weeks to the rats. The total volume of testis, volume of interstitial tissue, volume of seminiferous tubules, diameter of seminiferous tubules and germinal epithelium thickness were evaluated using stereological methods. Hormones were also measured and the results were analyzed using one way ANOVA.
Results: A significant reduction of total volume of testis (p<0.01), mean volume of seminiferous tubules (p<0.002) as well as germinal epithelium thickness (p<0.05) in both vasectomy + sodium arsenite and vasectomy rats was seen compared to sham operated only. In addition a significant reduction of testosterone was observed in vasectomy + sodium arsenite group when compared to the other groups (p<0.001). LH level decreased significantly in vasectomy + sodium arsenite when compared to sham operated ones (p<0.05).
Conclusion: Vasectomy and treatment with sodium arsenite affect the structure of testis with respect to its volume, volume of seminiferous tubules and thickness of germinal epithelium, which may be due to variation of LH and testosterone level in the rats.
Tahmineh Peirouvi, Gholamhossein Farjah, Jafar Soleimani Rad, Marefat Ghaffari Novin,
Volume 5, Issue 4 (7-2007)

Background: Phospholipids are distributed asymmetrically between inner and outer leaflets of the plasma membrane of live cells. Early during apoptosis, this asymmetry is disrupted and phosphatidylserine becomes exposed on the outside surface of the plasma membrane. There is little information about the effects of vitrification on apoptosis.
Objective: The aim of the present study was to evaluate the effect of vitrification on apoptosis of subfertile and fertile men.
Materials and Methods: In this study, semen samples were collected from subfertile (n=20) and fertile men (n=10) after 48h abstinence of intercourse. After semen analysis according to WHO criterias, each semen sample was divided into two portions. First portion was assessed by Annexin V-flous staining kit for showing apoptosis in subfertile and fertile men and second portion was assessed after vitrification-thawing. Results were analyzed by Paired t-test and Independent t-test.
Results: After vitrification-thawing, mean percentage of apoptotic spermatozoa has increased 6 and 3 times in subfertile and fertile men respectively. This difference is significant.
Conclusion: Vitrification-thawing could disrupted membrane asymmetry and caused apoptosis. Therefore, it will cause reduction of functional spermatozoa in access of Assisted Reproduction Technologies (ART).
Abdolhossein Rezaeian, Seyed Mehdi Kalantar, Safar Farajnia, Mehrdad Soleimani, Abbas Baghi, Abbas Aflatoonian, Sirous Zeinali,
Volume 5, Issue 5 (7-2007)

Mohammad Reza Moein, Mehrdad Soleimani, Nasim Tabibnejad,
Volume 6, Issue 3 (7-2008)

Background: Reactive oxygen species (ROS) are a group of free radicals that in excessive amounts have negative influence on sperm quality and function.
Objective: To study the effect of varicocele and its severity on the level of ROS in infertile men with clinical varicocele.
Materials and Methods: In this controlled prospective study, 42 men with clinically diagnosed left varicocele and 30 fertile men were studied. Patients were asked about history of urogenital infection, using any antioxidant medication and smoking. Grade of varicocele was determined by physical examination. Levels of ROS in seminal plasma were measured in each group by a chemiluminiscence assay. The sperm parameters were also determined and compared in different groups.
Results: The ROS levels were significantly higher in patients with varicocele than normal men (mean: 1575.42 RLU (Radio Luminescence Unit) vs. 53.79 RLU, p=0.005). In total 20 patients had grade I, 20 patients grade II and 2 patients had grade III varicocele. The mean ROS levels were 669.12 RLU, 2406.87 RLU and 2324 RLU in patients with grade I, II and III varicocele respectively (p=0.144). In case group, 15 patients were smoker and 27 were non-smokers. The mean ROS levels in patients with the history of smoking was 1367.71 RLU while in non-smokers it was 897.672 RLU (p=0.729). Conclusion: Our study showed that increased levels of ROS production in the seminal fluid can be an important factor in the etiology of male infertility in patients with varicocele, and this effect is more prominent with higher grade of varicocele.
Elke Heytens, Reza Soleimani, Petra de Sutter,
Volume 6, Issue 5 (7-2008)

Fertilization is triggered by changes in intracellular calcium concentration. In mammals, these transients in ooplasmic calcium concentration take the form of repetitive spikes, so called calcium oscillations (Ca2+-oscillations). These oscillations are important for relieve of meiotic arrest and to induce all the other events of oocyte activation. Although a surface mediated way of oocyte activation has been proposed, there is now substantial evidence to suggest that the sperm cell induces these Ca2+-oscillations by introducing a sperm specific phospholipase C, PLCζ, in the ooplasm. Ca2+-oscillations are also observed after intracytoplasmic sperm injection (ICSI), a successful technique in human assisted reproduction. In the rare cases that no fertilization is observed following ICSI, this may be due to a deficiency in PLCζ. However, artificial activating the oocytes after ICSI by increasing the calcium concentration can restore fertilization rates in these cases and support further development, as evidenced by successful pregnancies. Further evaluation of the current protocols for assisted oocyte activation is appropriate and investigation of the future application of PLCζ is warranted.
Leila Roshangar, Jafar Soleimani Rad, Parisa Nikpoo, Manijeh Sayyah Melli,
Volume 7, Issue 2 (7-2009)

Background: Induction of ovulation in ART is necessary for superovulation and the side effects of superovulatory drugs are debated. Oxytocin as a natural hormone, have receptors and is synthesized by several reproductive organs. Preovulatory presence of oxytocin receptor mRNAs in granulosa cells indicating a role for oxytocin in follicular development.
Objective: The aim of the present study was to investigate the effect of exogenous oxytocin injection on folliculogenesis, ovulation and endometrial growth in mice.
Materials and Methods: Forty adult female mice were divided into two groups as control and experimental. The mice at their sterous cycle received 1 IU/gr oxytocin, in experimental, and the same volume of solvent in control groups. Half of the mice in each group are sacrificed at 24 hours post injection and the other half, 48 hours after the injection. Ovarian samples fixed in 10% formalin, embedded in paraffin and sections were stained with H and E and studied using stereological techniques. The data were analyzed with Man– Whitney test.
Results: Microscopic examination revealed that the number and morphological features of follicles at different stages were similar at 24 and 48 hours post injection in both groups. The volumes of the ovaries were similar in both groups at 24 hour. However, at 48 hour, the volume of ovaries, corpora lutea and endometrial thickness, in experimental group, were significantly higher than those in control group (p< 0.05).
Conclusion: According to the increased volume of corpus luteum in the experimental group, it is concluded that oxytocin injection has a stimulatory effect on induction of ovulation.   
Hamid Reza Momeni, Malek Soleimani Mehranjani, Mohammad Hosien Abnosi, Monireh Mahmoodi,
Volume 7, Issue 3 (7-2009)

Background: para-nonylphenol (p-NP) is able to induce malformations in male reproductive system.
Objective: The aim of this study was to investigate the preventing role of vitamin E (Vit.E) on sperm parameters and reproductive hormones in developing rats.
Materials and Methods: Pregnant rats were divided into 4 groups: control p-NP Vit.E and p-NP+Vit.E. Treatments were performed on day 7 of gestation and continued during weaning. The male pups were then divided into the same groups as the mothers and were treated till 90 days of age. Finally body and left testis weight were recorded and left epididymis was cut in Ham’s F10. Released sperm were used to analyze number motility and viability of the sperm. Blood serum was used to assess follicle stimulating hormone (FSH) luteinizing hormone (LH) estrogen and testosterone.
Results: In p-NP-treated rats a significant decrease was found in body and testis weight sperm number and sperm motility compared to control and p-NP+Vit.E groups. A significant increase was also found in sperm viability in Vit.E group (83.3±7.6) compared to both p-NP (59.5±7.5) and control (66.3±9.7) groups. Rats treated with p-NP showed a significant decrease in FSH level and a significant increase in estrogen level. However testosterone and LH level remained constant. In p-NP+Vit.E group the change of estrogen level but not FSH was significantly reversed compared to p-NP group. Conclusion: Vit.E not only is able to compensate the toxic effects of p-NP on testis weight sperm number sperm motility and estrogen level but also increases sperm viability in developing rat.
Marzieh Panahi, Azam Soleimani, Abdolrahman Abdolrahman Dezfoolian,
Volume 7, Issue 4 (7-2009)

Background: Acceptance of uterus and reaction between endometrium and embryo has an important role for implantation. Muc1 an integral membrane mucin is expressed on the apical surface of uterine epithelial cells and could have effects on its receptivity.
Objective: The aim of this study was to evaluate the changes in Muc1 expression of gravid mouse endometrium with and without hyperstimulation before implantation.
Materials and Methods: Adult female NMRI mice were divided into control and experimental groups. Experimental group superovulated using an intraperitoneal injection of Pregnant Mare’s Serum Gonadotrophin (PMSG) followed 48 hours later by another injection of Human Chorionic Gonadotropic hormone (HCG). The female mice have mated with normal male mice. All control and hyperstimulated groups subdivided into six groups. After mating female mice were examined by vaginal plaque as day of zero and in 0-5 days after copulation they were sacrificed by cervical dislocation. Then the middle 1/3 parts of their uterine horns were obtained and stained by immunohistochemicaly technique for Muc-1 detection.
Results: Our results showed that in the control and hyperstimulated groups the Muc1 expression is markedly reduced in the luminal uterus epithelium at the time of implantation. Furthermore luminal and glandular uterus epithelium did not exhibit the same decrease in Muc1 expression during the receptive phase.
Conclusion: Ovarian hyperstimulation didn’t alter the Muc1 expression markedly in surface and glandular epithelium of endometrium which could affect on its receptivity.
Malek Soleimani Mehranjani, Ali Noorafshan, Ahmad Hamta, Hamid Reza Momeni, Mohammad Hussein Abnosi, Monireh Mahmoodi, Morteza Anvari, Maryam Hazaveh,
Volume 8, Issue 1 (7-2010)

Background: Para-Nonylphenol (p-NP) is one of the environmental pollutants which cause reproductive system disorders.
Objective: The effects of vitamin E on ovary structure during its development in rats treated with p-NP.
Materials and Methods: 32 Wistar female rats after mating were divided into 4 groups; control vitamin E (100mg/kg/day) p-NP (250mg/kg/day) and p-NP + vitamin E. The rats were treated from the day 7 of pregnancy till 21st day of postnatal through sucking period. After weaning the female pups were treated by gavages for 120 days. The total volume of ovary number of follicles volume of oocyte follicular cells and their nuclei and the thickness of zona pellucida were estimated stereologically. The results were analyzed using one way ANOVA and p<0.05 was considered significant.
Results: The ovary weight, mean total volume of ovary and cortex, number of antral and graafian follicles and body weight were decreased significantly (p<0.05) in the p-NP treated rats compared to control and other groups, while the number of atretic follicles was increased significantly (p<0.05). A significant reduction (p<0.05) in volume of oocyte, follicular cells and their nuclei in antral and graafian follicles was found in p-NP group. In addition, treatment with only vitamin E showed an improving effect on folliculogenesis due to a highly significant increase (p<0.01) in the number of primordial follicles.
Conclusion: Vitamin E could compensate the adverse effects of p-NP on the ovary structure during its development.
Elham Aliabadi, Malek Soleimani Mehranjani , Zahra Borzoei, Tahereh Talaei-Khozani, Hossein Mirkhani, Hamed Tabesh,
Volume 10, Issue 2 (7-2012)

Background: Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality.
Objective: The aim of this investigation was to evaluate the improvement of the testicular sperm motility and chromatin quality exposed to L-carnitine (LC) and L-acetyl-carnitine (LAC), which are normally concentrated in testis and epididymis, compared with Pentoxifylline (PF), which used for sperm motility enhancement in IVF procedures.
Materials and Methods: TESE samples from 30 male mice divided into four parts. The sperm samples were added to Hamchr('39') F10 (control) or the media contained 1.76mM of LC, LAC or PF), then, the samples were kept in the room temperature for 30, 90 and 180 min. At each time step, sperm motility and chromatin quality were assessed. Chromatin quality was evaluated by chromomycin A3 and aniline blue. Statistical analysis was performed using one way analysis of variance (ANOVA). A p-value less than 0.05 were accepted as a statistically significant difference.
Results: The results showed LC, LAC and PF significantly increased the sperm motility. However, sperm chromatin quality only improved significantly by administration of LC and LAC.
Conclusion: Administration of LC and LAC to the testicular sperm samples can lead to improve both sperm motility and chromatin quality. It may be because they can mimic in vivo sperm condition during late spermatogenesis.
Korosh Khanaki, Ali Motavalizadeh Ardekani, Alieh Ghassemzadeh, Vahideh Shahnazi, Mohammad Reza Sadeghi, Masoud Darabi, Amir Mehdizadeh, Abotaleb Saremi, Jafar Soleimani-Rad, Ali Reza Imani, Mohammad Nouri, Ali Rahimipour,
Volume 10, Issue 4 (8-2012)

Background: Endometriosis is a common chronic inflammation which leads to infertility and chronic pelvic pain in affected women. Secretory phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is markedly increased in inflammatory disorders.
Objective: To assess the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) administration in endometrial cells culture on sPLA2IIa level and cell survival comparing homolog ectopic versus eutopic endometrial cells from endometriosis patients.
Materials and Methods: In this experimental study, ectopic and eutopic endometrial tissue samples obtained from 15 endometriosis patients were immediately frozen. After thawing and tissue digestion, mixed stromal and endometrial gland cells were cultured for 8 days in three different culture media; balanced ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell survival was measured using 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H- tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with ELISA technique.
Results: The sPLA2IIa level was significantly higher in the ectopic endometrial cell culture compared to the eutopic group for each of the three matched treatments (balanced, high ω-3 and high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell group was remarkably increased by each of the three PUFAs treatments compared to control condition (p<0.05, p<0.01, p<0.05 respectively). Cell survival in the eutopic group was significantly decreased by high ω-6 culturing compared to control medium (p<0.05).
Conclusion: The increase in sPLA2IIa level in ectopic endometrial cells by fatty acid treatments (especially high ω-3), strengthens the hypothesis that PUFAs stimulate secretion of cytokines leading to increased sPLA2IIa level.
Malek Soleimani Mehranjani, Rezvan Taefi,
Volume 10, Issue 6 (4-2012)

Background: Vitamin E is an effective antioxidant, protecting cells against oxidative stress.
Objective: In this investigation the protective effect of vitamin E on the testis during development and spermatogenesis in rats exposed to sodium arsenite was evaluated.
Materials and Methods: Pregnant Wistar rats were divided into 4 groups (n=8) control, sodium arsenite (8 mg/kg/day), sodium arsenite+vitamin E (100 mg/kg/day) and vitamin E. Treatment was carried out from day seven of pregnancy till 90 days. Finally the right testis was stereologically studied. The obtained data was analyzed using one way ANOVA and Tukeychr('39')s test and the means difference was considered significant at p<0.05.
Results: The weight and volume of testis, volume of seminiferous tubules and its diameter, volume of interstitial tissue, height of germinal epithelium and the total number of types A and B spermatogonia, spermatocyte, spermatid and sertoli cells reduced significantly in sodium arsenite group compared to the control. Co-administration of vitamin E and sodium arsenite compensated the adverse effects of sodium arsenite on the above parameters.
Conclusion: We concluded co-treatment of rats with sodium arsenite and vitamin E could prevent the adverse effects of sodium arsenite exposure on the testicular tissue during the prenatal stage till sex maturity.
Ali Reza Navabazam, Fatemeh Sadeghian Nodoshan, Mohammad Hasan Sheikhha, Sayyed Mohsen Miresmaeili, Mehrdad Soleimani, Farzaneh Fesahat,
Volume 11, Issue 3 (5-2013)

Background: Human dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue.
Objective: The aim was to isolate human dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC) and periapical follicle stem cells (PAFSC) for their potential role in tissue regeneration.
Materials and Methods: In this experimental study, the postnatal stem cells were isolated from dental pulp, preapical follicle and periodontal ligament .The cells were stained for different stem cell markers by immunocytochemistry. To investigate the mesenchymal nature of cells, differentiation potential along osteoblastic and adipogenic lineages and gene expression profile were performed. For proliferation potential assay, Brdu staining and growth curve tests were performed. Finally, all three cell types were compared together regarding their proliferation, differentiation and displaying phenotype.
Results: The isolated cell populations have similar fibroblastic like morphology and expressed all examined cell surface molecule markers. These cells were capable of differentiating into osteocyte with different capability and adipocyte with the same rate. PAFSCs showed more significant proliferation rate than others. Reverse transcriptase PCR (RT-PCR) for nanog, oct4, Alkaline phosphatase (ALP) and glyceraldehydes-3-phosphate dehydrogenease (GADPH) as control gene showed strong positive expression of these genes in all three isolated cell types.
Conclusion: PDLSCs, DPSCs and PAFSCs exist in various tissues of the teeth and can use as a source of mesenchymal stem cells for developing bioengineered organs and also in craniomaxillofacial reconstruction with varying efficiency in differentiation and proliferation.
Mohammad Hasan Sheikhha, Mohammad Ali Zaimy, Saeede Soleimanian, Seyed Mehdi Kalantar, Azam Rasti, Maryam Golzade, Hamid Hoseini Fahraji,
Volume 11, Issue 4 (6-2013)

Background: It has been hypothesized that Y-q microdeletion can account for significant proportion of infertility in men. There are three nonoverlapping regions referred to as the "azoozpermia factors" AZFa, AZFb, and AZFc from proximal to distal part of Y-q. These have been defined as spermatogenesis loci, this region deletions have been shown to be involved in male azoospermic or severe oligoozospermic infertility.
Objective: Evaluation the rate of Y-chromosome microdeletions in infertile men.
Materials and Methods: In this case-control study, 25 azoospermic infertile men candidate for intracytoplasmic sperm injection (ICSI) were selected as case group. For control group, 25 normoozoospemric men were selected. All cases and controls had normal 46XY karyotype. DNA extraction and molecular analysis were done on blood samples. Multiplex-PCR method was done to identify the presence of microdeletion in AZFa, AZFb or AZFc loci. Eight STS primers that include two controls were selected to determine Y-chromosome microdeletions.
Results: 20% (5/25) of all patients have at least one microdeletion in more than one region of AZF loci. Totally 17 microdeletions was observed, one case had deletions in three AZF regions, and 4 cases had deletions in two AZF regions. The rate of deletions was 42% (7/17) for AZFc, 35% (6/17) for AZFa and 23% (4/17) for AZFb.
Conclusion: The molecular DNA analysis could help us to know the real cause of infertility and can give good information for good decision for example in men whit microdeletions who want to undertake ICSI procedure the deletions will be passed to their son.
Saeede Soleimanian, Seyyed Mahdi Kalantar, Mohamad Hasan Sheikhha, Mohamad Ali Zaimy, Azam Rasti, Hossein Fazli,
Volume 11, Issue 5 (7-2013)

Background: In human, about 25% of implanted embryos are losing 1-2 week following attachment to the uterus. A subset of this population will have three or more consecutive miscarriages which define as repeated pregnancy loss (RPL). Introducing the assisted reproductive technologies (ARTS) made a chance for infertile couples to solve their childless problem.
Objective:  This study was conducted to evaluate the incidence of Y-chromosome AZF regionchr('39')s micro-deletions in male partners of couples with recurrent miscarriage (RM).
Materials and Methods:  Thirty male partner of couples with RM and thirty infertile males, who referred to the Yazd Research and Clinical Center for Infertility were recruited to this study. In addition, 30 healthy men were screened as a control group from the same center. After DNA extraction using salting out method, the multiplex-PCR was done for amplifying 8 known STSs proximal to the AZF region of the Y-chromosome. The results were compared between the groups using Fisherchr('39')s exact t-test and p<0.05 was considered statistically significant.
Results:  Of the 30 infertile males, 5 (16.6%) cases were associated with the AZF region micro-deletions of DYF87S, DYF84S1, DYF83S1 and DYF51S1, STSs. But in the fertile and RM male groups was found no deletions similar to those, of the infertile males (p=1.0). Instead 4 (13.3%) cases of the RM group males had different micro-deletions included DYS220 (AZFb, sY129), DYS262, DYF8551, and DYF8651, STSs. The AZFc locus of Y-chromosome micro-deletions have a significant role in RM (p=0.045).
Conclusion:  It seems that the Y-chromosome AZF regionchr('39')s micro-deletions are associated with RM, and we recommend adding this AZF region STSs into infertility analyzing panels.
Roshangar Leila, Seddighe Abdollahifard, Abbas Majdi, Armin Zarrintan, Alia Ghasemzade, Laaia Farzadi, Sara Soleimani Rad, Jafar Soleimani Rad,
Volume 11, Issue 5 (7-2013)

Background: More than 40% of infertilities are due to endometriosis. Ultrustructural and histochemical study of endometrium will help to clarify the etiology of endometriosis.
Objective: The aim of the present study was to investigate the ultrastructure and occurrence of apoptosis in endometrial cells of women with or without endometriosis.
Materials and Methods: In the present case-control study, endometrial specimens from 12 women without endometriosis (as control) and 12 women with endometriosis (as case) were examined. Specimens for control group were obtained from the patients that were referred to gynecology hospital for hysterectomy due to various reasons. In case group the endometriosis was diagnosed according to laparoscopy and endometrial samples were taken using pippel biopsy. The specimens from both case and control groups were processed for Transmission Electron Microscopy (TEM), TUNEL reaction technique and morphometric studies.
Results: The results show that endometrial epithelium lost its continuity in women with endometriosis and endometrial cells have euchromatic nucleus in comparison to those from non-endometriosis. There were several apoptotic cells in the luminal and glandular endometrial epithelium and stroma from endometrium of control group. However, apoptotic cells were rarely seen in the endometrium from women with endometriosis. The difference in number of apoptotic cells between two groups statically was significant (p<0.001).
Conclusion: Regarding the ultrastructural characteristics of endometrial epithelial cells and comparison of apoptotic occurrence in control and case groups it is concluded that endometrial cells in endometriosis group have higher potential to survive and possibly implant.
Leila Roshangar, Jafar Soleimani-Rad, Bahman Rashidi, Hossein Mazochian, Behzad Nikzad, Sara Soleimani Rad,
Volume 11, Issue 10 (12-2013)

Background: Endometrial development has an important role in blastocyst adhesion and implantation. During IVF cycles, endometrial development is enhanced by progesterone.
Objective: The aim of this study was to compare ultrastructural and morphometrical characteristics of mice uterine endometrium in natural cycle with those in superovulated cycles received progesterone or Sildenafil.
Materials and Methods: In This study, 60 female bulb/c mice were divided into 4 groups: a control and 3 experimental; gonadotropin, gonadotropin+ Sildenafil and gonadotropin+ progesterone. In experimental groups the mice superovulated mated. In the gonadotropin+ progesterone and gonadotropin+ Viagra groups, the mice respectively received 1mg progesterone and 3 mg Sildenafil citrate. Their uterine specimens were prepared for morphometrical and ultrastructural study. Height of the epithelial cells was measured, using motic software. Statistical analysis was performed using ANOVA.
Results: Microscopy revealed that in control group the cells had numerous apical microvilli and the height of the cells was 20.52±2.43 μm. In gonadotropin+ progesterone group, the granules were found in basal and apical portions and cellular height were 17.91±2.78 μm which were significantly shorter than in the control and gonadotropin groups (p<0.001). In this group, the apical membrane also contained pinopodes. In gonadotropin +Sildenafil group, the granules were found in both apical and basal portions and the height of the cells were 17.60±2.49 μm which were significantly shorter than in the control and gonadotropin groups (p<0.001). In this group, pinopodes appeared slightly extensive than the other groups.
Conclusion: It is concluded that superovulatory drugs in mice stimulate endometrial maturation but injection of Sildenafil is nearly more positive.
Azita Faramarzi, Mohammad Ali Khalili, Mehrdad Soleimani,
Volume 13, Issue 4 (5-2015)

Background: Embryo selection is a vital part of in vitro fertilization (IVF) programs, with morphology-based grading systems having been widely used for decades. Time-lapse imaging combined with embryo morph kinetics may proffer a non-invasive means for improving embryo selection. We report the first ongoing and chemical pregnancies using Time-lapse embryo scope to select best embryos for transfer in Iran.
Cases: A case with tubal factor infertility was admitted to IVF program with normozoospermia. After ovarian hyper stimulation, 6 COCs were retrieved and inseminated with 25,000 progressive sperms/ oocyte. Five zygotes were placed individually into the micro wells of equilibrated embryo scope dish for Time-lapse observation, and incubated at 37°C, 5% CO2. On day 3, single embryo transfer (SET) took place based on kinetic parameters of the embryos. Clinical pregnancy was confirmed 7 weeks after SET. The second case with history of previous ICSI failure was admitted with azoospermia. Nine MII oocytes underwent ICSI, and incubated in Time-lapse facilities. The rest of procedures were followed as described for case 1. Chemical pregnancy was confirmed 15 days after SET.
Conclusion: This approach opens a way to select best embryo non-invasively for SET; thus, increasing implantation, while reducing multiple pregnancy complications.
Sara Soleimani Rad, Shamsi Abbasalizadeh, Amir Ghorbani Haghjo, Mehzad Sadagheyani, Azadeh Montaseri, Jafar Soleimani Rad,
Volume 13, Issue 7 (9-2015)

Background: Infertility is defined as the inability to achieve the pregnancy within a year of unprotected intercourse. Infertility is a complex issue and different factors such as stress oxidative can be involved in this problem. So, any attempt to neutralize oxidative stress would be helpful in the treatment of infertility. Melatonin is a known scavenger of free radicals.
Objective: The aim of our study was to evaluate the level of melatonin and its correlation with oxidative biomarkers in fertile and infertile women.
Materials and Methods: The participants including fertile and infertile women were divided into two groups of 30 people. Blood sampling was performed and sera were collected. The level of Malondialdehyde (MDA), total antioxidant capacity (TAC) and melatonin were detected. Data were analyzed using T-test and their correlation was assessed using Spearman test.
Results: Serum melatonin from fertile women was higher than infertile women but the difference was not significant (p= 0.46). MDA level in fertile women was significantly lower than infertile women (p<0.001) and the level of TAC in fertile women was significantly higher than infertile women (p<0.001). Spearman test revealed a significant and direct correlation between melatonin and TAC in fertile and infertile women and a significant but reverse correlation between melatonin and MDA in infertile and fertile women.
Conclusion: Differences in the level of oxidative stress biomarkers in fertile and infertile individuals have been reported. This study revealed a significant correlation between melatonin and oxidative stress biomarkers, concluding that melatonin level could be involved in infertility.

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