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Showing 6 results for Singh

Sundararajan Venkatesh, M.pharm Gurdeep Singh, Narmada Prasad Gupta, Rajeev Kumar, Munusamy Deecaraman, Rima Dada,
Volume 7, Issue 1 (7-2009)

Background: Excess reactive oxygen species (ROS) in the semen is believed to affect fertility in men. Morphologically abnormal sperms and their relation to seminal oxidative stress in infertile and subfertile men are not clear.
Objective: To correlate various sperm morphological defects with seminal oxidative stress in infertile and subfertile men.
Materials and Methods: The study included 25 primary, 21 secondary infertile men of idiopathic infertility and 15 fertile controls. Standard semen analysis was performed according to WHO (1999) guidelines. Sperm inter-morphological defects were evaluated in 100 sperms per sample by Giemsa staining. ROS in spermatozoa was measured by the chemiluminescence assay.
Results: Significant difference in percent sperm amorphous head was found between secondary infertile group and control men. The study showed a significantly higher percent spermatozoa with residual cytoplasm between primary [11.61 (6.6, 3.9)], secondary [7.49 (0.8, 13)] and fertile controls [2.44 (0.8, 3.7)] similar to sperm count, percent sperm progressive motility, and ROS levels. A non significant but strong positive correlation (r=0.3479, p=0.0884) between percent cytoplasmic retained spermatozoa and ROS levels was observed in the primary infertile group. However, no correlation between other sperm morphological defects and oxidative stress was observed.
Conclusion: Sperm morphology was not found to be associated with oxidative stress in the present study. However, retained cytoplasmic residues in the sperm may be an important source of ROS in both primary and secondary infertile men. These immature spermatozoa are believed to be associated with impaired fertility.
Bineeta Kashyap, Namita Srivastava, Iqbal R Kaur, Rajat Jhamb, Deepak K Singh,
Volume 11, Issue 7 (10-2013)

Background: Tuberculosis (TB) is an increasing public health concern worldwide. On a global scale it has a devastating impact in developing nations. Genital TB, an extrapulmonary form, is not uncommon particularly in areas where pulmonary TB is prevalent. Genital TB may be asymptomatic or may even masquerade as other gynaecological conditions; hence, diagnosis requires a high degree of suspicion and the use of appropriate investigations.
Objective: This study attempted to identify endometrial TB in endometrial biopsies taken from women evaluated for infertility by comparison of various staining techniques.
Materials and Methods: A comparative cross sectional study was conducted from February 2011 to April 2011 in Guru Teg Bahadur Hospital, New Delhi. Endometrial biopsy specimens from 55 endometrial TB suspects were stained for acid fast bacilli by Ziehl Neelson staining and Gabbet staining. The biopsy samples were also subjected to Auramine Phenol fluroscent staining and H and E staining. Culture on Lowenstein Jensen medium was taken as the gold standard.
Results: Three samples were culture positive giving positivity rate of 5.4%. Considering culture as the gold standard the senstivities of ZN, Gabbet, fluorescent and H and E staining were 33, 33, 66, and 66% respectively while their specificities were 100, 100, 98, and100% respectively.
Conclusion: Combination of fluorescent staining techniques along with one of the acid fast staining techniques or histopathology achieves sufficient sensitivity and specificity for the diagnosis of female genital tuberculosis. There is an urgent need for developing definitive diagnostic methods to make a conclusive diagnosis of genital TB.
Siti Syairah Mohd Mutalip, Gurmeet Kaur Surindar Singh, Aishah Mohd Shah, Mashani Mohamad, Vasudevan Mani, Siti Nooraishah Hussin,
Volume 11, Issue 8 (11-2013)

Background: Anabolic androgenic steroids (AAS) is being used in medical treatments, but AAS also was identified to have the risks of adverse effects towards patients and consumers health.
Objective: Present study was conducted to observe the effects of testosterone, nandrolone, and stanozolol (forms of AAS) intake during onset of puberty on the rat testicular histology.
Materials and Methods: Juvenile male Sprague-Dawley (SD) rats (n=42) were divided into seven groups and were injected subcutaneously with medium dose of polyethylene glycol-200 (PEG-200) (control), testosterone, nandrolone, and stanozolol for six weeks (PND 41-87). The animals were weighed daily and sacrificed on PND 88. Testes were removed, weighed, and prepared for histological assessment and finally specimens were observed under microscope.
Results: The results showed an insignificant increase in mean daily body weight with highest and lowest body weight gained was of 177.6±1.69 gr and 140.0±12.26 gr respectively. There was significant decrease in the testes absolute weight (p≤0.01) in all experimental groups except in the nandrolone 2.5 mg/kg/week treated group. Testicular histology of rats treated with AAS also showed slight changes in the uniformity of arrangements of seminiferous tubules.
Conclusion: Data from present study suggests that AAS have been initiating the adverse effects on testicular normal functions in rats during onset of puberty.
Swapnil Singh, Shilpa Bhandari, Pallavi Agarwal, Priya Chittawar, Ratna Thakur,
Volume 14, Issue 3 (3-2016)

Introduction: Chlamydia is an important cause of sexually transmitted diseases leading to tubal factor infertility.
Background: This study aims to define the role of chlamydial antibody detection in predicting presence, nature and type of tubal pathology in laparoscopy.
Materials and Methods: A prospective study was conducted on 200 consecutive patients undergoing laparoscopy as a part of infertility work-up. Preoperatively, serological determination of Immunoglobulin G (IgG) specific antibodies against Chlamydia Trachomatis was done by Enzyme linked immunosorbant assay (ELISA). Findings of laparoscopy were evaluated against presence or absence of chlamydial antibodies in serum.
Results: Out of 200 patients,10 patients tested positive for chlamydial antibody. Chlamydial antibody was found positive in 20% and 22.7% of patients with tubal pathology and peri-hepatic adhesions of patients, respectively. The sensitivity of chlamydial antibody for diagnosing tubal pathology was found to be 20%, while specificity was 100%. The positive chlamydial antibody test was not statistically associated with involvement of one or both tubes and site of tubal block.
Conclusion: Chlamydia antibody test does not appear to be good screening test for tubal pathology especially in Indian subcontinent. In view of its high specificity, this test can be used to identify patients with higher chances of tubal pathology requiring operative intervention.
Gautam Dagur, Kelly Warren, Navjot Singh, Sardar Ali Khan,
Volume 14, Issue 5 (5-2016)

Seminal vesicles (SVs) are sex accessory organs and part of male genitourinary system. They play a critical role in male fertility. Diseases of the SVs, usually results in infertility. Diseases of the SVs are extremely rare and are infrequently reported in the literature. We address the current literature of SV pathologies, symptoms, diagnosis, and treatment options. We review the clinical importance of SVs from PubMed. The current imaging modalities and instrumentation that help diagnose SV diseases are reviewed. Common pathologies including, infection, cysts, tumors, and congenital diseases of the SVs are addressed. Many times symptoms of hematospermia, pain, irritative and obstructive lower urinary tract symptoms, and infertility are presented in patients with SV diseases.
Naina Kumar, Amit K Singh, Ajay R Choudhari,
Volume 15, Issue 8 (9-2017)

Background: High rates of sub-fertility and adverse pregnancy outcomes were seen after age 40. In contrast to oogenesis, spermatogenesis continues in elderly men.
Objective: To retrospectively study the impact of aging on semen parameters in male partners of infertile couples in the rural area of developing country over 10 years and to find out whether aging affects male factor fertility and various semen parameters in this part of developing country.
Materials and Methods: In this cross sectional study, the laboratory semen analysis records of 1219 male partners of infertile couples of a rural tertiary care center of Central India in a 10-year period from January 2005 to December 2014 were evaluated into 5 groups based on men age: Group 1: 21-28 yr (n=57); group 2: 29-35 yr (n=450); group 3: 36-42 yr (n=532); group 4: 43-49 yr (n=165), and group 5: 50-60 yr (n=15). Evaluation of all semen parameters were done according to WHO standard criteria (1999).
Results: The analysis of semen records revealed the significant negative association of semen volume, total sperm count, sperm motility, and morphology with age. There was a significant fall in total sperm count, sperm motility, and morphology after the age of 35 yr.
Conclusion: Age has significant negative effect on semen volume, total sperm count, and sperm motility and morphology in this region of India.

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