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Showing 4 results for Rezazadeh

Behnaz Sheikholslami, Mojdeh Salehnia, Mojtaba Rezazadeh,
Volume 2, Issue 1 (7-2004)

The cytokine of granulocyte macrophage colony stimulating factor (GM-CSF) is a glycoprotein, which is synthesized in the female reproductive tract and has embryonic trophic effect in mammals. The objective of this study was to examine the optimal dosage of GM-CSF to improve the mouse embryo development in vitro. To collect two and eight cells embryos, the pregnant NMRI mice were sacrificed by cervical dislocation at 48 h and 72 h post hCG injections, respectively. The embryos were cultured randomlly in T6 medium supplemented with 5 mg /ml bovine serum albumin (BSA) and 0, 2, and 10 ng / ml human rGM-CSF. The data of blastocyst formation and hatching in different groups of embryo culture were compared by chi-square analysis. The results showed that the developmental rates of 2 and 8 cells embryos to hatching blastocyst in the presence of 2 ng/ml of GM-CSF their control groups (51.5% and 49.7%, respectively) were more than those in the other groups, but insignificant. It seems more researches are necessary to confirm this suggestion that the GM-CSF with 2 ng/ml concentration may have a better potential, not only to enhance the developmental rates of 2 and 8 cells embryos but also for decreasing the degeneration of those embryos.
Fatemeh Peyghambari, Mojdeh Salehnia, Mehdi Forouzandeh Moghadam, Mojtaba Rezazadeh Valujerdi, Ebrahim Hajizadeh,
Volume 6, Issue 4 (7-2008)

Background: The preparation of endometrium for embryo reception and implantation are controlled by ovarian hormones. These hormones have distinct cyclical changes during estrus cycle.
Objective: The aim of this study was to evaluate the changes in morphology and morphometrical indices of endometrium by daily injections of estrogen and progesterone in ovariectomized mouse.
Materials and Methods: In total 60 adult NMRI female mice were ovariectomized and after two weeks, they were randomly divided into five groups: control, sham group, estrogen treated mice (which received daily dosage of 0.5 ml/mouse of hormone for five days), progesterone treated mice (which received daily dosage of 0.2 ml/mouse of progesterone hormone for five days) and estrogen-progesterone treated mice (they received 0.5 ml/mouse estrogen on the first day and 0.2 ml/mouse progesterone injections from the second day to the fifth day of treatment). The mice were sacrificed in every day (n=5) up to five days after treatment and their uterine horns were obtained and processed for morphological and morphometrical studies.
Results: On the second day of treatment, the diameter of glands was observed to be more in the progesterone group (53.75±6.32μ) than this in the estrogen (45.13±7.78 μ) and estrogen-progesterone treated groups (48.17±13.58 μ). While, the number of glands (76.25±17.37) and thickness of endometrium (39.58±3.37 μ) were observed to be more in the estrogen treated group (p=0.01).
Conclusion: Progesterone had effect on the gland whereas estrogen caused increased in height of surface epithelium of endometrium. Overall, the day 2 after treatment (in all experimental groups) is suitable day for sampling for further studies.
Kamran Haidari, Mojdeh Salehnia, Mojtaba Rezazadeh Valojerdi, Shahram Pourbyranvand,
Volume 6, Issue 5 (7-2008)

Background: The ultrastructural analysis of cultured follicles could direct us to understand subcellular changes during in vitro culture.
Objective: This study was done to verify the ultrastructural characteristics of in vitro cultured mouse isolated preantral follicles in co-culture system in the presence and absence of leukemia inhibitory factor (LIF)
Materials and Methods: Mechanically isolated preantral follicles were divided into four groups: control without LIF, control with LIF, co-cultured group with LIF, co-cultured group without LIF. In co-culture groups the follicles were cultured with cumulus cells. After 4 days the follicles were processed and sectioned for transmission electron microscopic examination.
Results: The oocytes of cultured preantral follicles in all studied groups demonstrated a homogeneous cytoplasm and they had the round or ovoid shaped mitochondria with light matrix and cristae. Their endoplasmic reticulum cisternae were in association with mitochondria and Golgi complex. The cortical granules and the aggregation of mitochondria around the germinal vesicle were prominent in both co-cultured groups. The organelle distribution in granulosa cells was normal in all groups of study and no sign of cell death was observed. In both co-cultured systems the granulosa cells contained mitochondria with tubular cristae, a well developed smooth endoplasmic reticulum and several large lipid droplets, characteristics of steroid synthesis cells. Conclusion: The oocyte and granulosa cells in co-cultured system showed more remarkable maturation features than that of control.
Farhad Golshan Iranpour, Mojtaba Rezazadeh Valojerdi,
Volume 11, Issue 3 (5-2013)

Background: When male animals die, spermatozoa within the body of animal will be degenerated. Because of unique chromatin structure of sperm, maybe this degeneration is different from other cells. However there is not any research which considered directly the integrity of sperm DNA by keeping the cadaver in refrigerator.
Objective: The aim of this study was to assess viability, total motility and DNA integrity of sperm cells after death.
Materials and Methods: In this experimental study, 24 male Swiss white mice were killed by cervical dislocation and then kept in refrigerator (4-6oC) for up to 12 days. On the 0 (immediately after death as control group), 1st, 2nd, 3rd, 5th, 7th, 10th and the 12th days after death cauda epididymides were removed and squeezed in Ham’s F10 medium. The proportion of viable, motile and double stranded DNA spermatozoa was examined. Viability and DNA integrity of sperm cells were examined consecutively by eosin nigrosin and acridine orange stainings.
Results: The data obtained from this study showed that viability and total motility of sperm cells were significantly decreased during 12 days after death (p<0.001). In contrast with viability and motility, DNA integrity was without significant changes (even 12 days after death).
Conclusion: This study suggests that integrity of sperm DNA would not change even after 12 days after death if the cadaver kept in refrigerator.

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