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Showing 11 results for Nouri

Mahmoud Salami, Zahra AghaNouri, Ali Akbar Rashidi, Mansoor Keshavarz,
Volume 2, Issue 2 (7-2004)

Background: Fetal alcohol syndrome is associated with numerous problems in the development and function of the brain. Learning and memory deficits are among well known effects of prenatal exposure to alcohol. Objective: This study aimed to examine the spatial working memory of 60-day old rats who were exposed to alcohol during their fetal life and to find the relation between the possible alcohol-impaired spatial memory and gestational period of exposure to alcohol. Materials and Methods: Pregnant rats in different stages of gestation period were administered with ethanol. Using a radial arm maze, the offspring were subjected to spatial working memory training at 60 days of postnatal age. Results: The rats exposed to ethanol during the first 10 days of fetal life indicated lower performances compared to the controls. Those receiving alcohol during the second half of pregnancy period had no problem in maze navigation. Behavior of the animals exposed to alcohol during the first and the second quarter of the gestation period demonstrated that only the latter were weak in solving maze tasks. The groups related to the third and the forth quarter of gestation period had a similar behavior with the control group. Comparison of the animals' performances in all groups revealed that only the second quarter group was the most disadvantaged. Conclusion: Our data indicates that the second quarter of the gestation period is more sensitive to harmful effects of alcohol on the areas of brain involved in learning and memory. Since the hippocampus is central in cognitive functions and this part of brain is highly vulnerable to alcohol effects it can be concluded that the hippocampus is mostly affected in the second quarter of prenatal life. Article
Parvin Gharabaghi, Seddighe Abdollahifard, Manizheh Gharabaghi, Mohammad Nouri,
Volume 5, Issue 5 (7-2007)

Background: The value of serial measurement of serum ß subunit of human chorionic gonadotropin (ßHCG) and ultrasonography in the early diagnosis of ectopic pregnancy has well established. Objective: The objective of this study was to explore the diagnostic value of raising level of serum ßHCG, single measurement of progesterone (P) and estradiol (E2) in early diagnosis of ectopic pregnancy. Materials and Methods: Serum levels of ßHCG and estradiol were measured by Radio Immuno Sorbent Assay (RIA) and progesterone level was measured by Enzyme Linked Immuno Sorbent Assay (ELISA) techniques in 43 symptomatic women with ectopic pregnancy and 42 women with normal intrauterine pregnancy in Alzahra Hospital, Tabriz, Iran. These values were compared by T-test. By determining cut-off levels of these parameters the efficiency and sensitivity of them in prediction of ectopic pregnancy was estimated. Results: The mean serum levels of ßHCG, estradiol and progesterone in patients with ectopic pregnancies (940 ± 552 mlu/ml, 593 ± 237 pg/ml, 5.83 ± 3.41 ng/ml, respectively) were significantly lower than these levels in normal intrauterine pregnancies (4620 ± 2030 mlu/ml, 1627 ± 435 pg/ml, 24.8 ± 6.08 ng/ml, respectively). The average rate of ßHCG rising was 8.2% for 24 hours in patients with ectopic pregnancy (EP) and 32.8% in normal intrauterine pregnancies (NIUP). Conclusions In this study single measurement of serum progesterone level has the greatest sensitivity (100%) and specificity (98%) in the diagnosis of ectopic pregnancy.
Mohammad Nouri, Aliyeh Ghasemzadeh, Laya Farzadi, Vahideh Shahnazi, Marefat Ghaffari Novin,
Volume 6, Issue 2 (7-2008)

Background: It has been shown that reactive oxygen species (ROS) can lead to deleterious effects on a range of sperm parameters. Vitamins E and C are capable of reducing ROS levels and lipid peroxidation. Objective: The aim of study was to determine the level of lipid peroxidation as indicated by Malondialdehyde (MDA) and vitamins E and C in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men and their relationships with semen parameters. Materials and Methods: Forty men with normozoospermic and 60 infertile men with asthenoteratozoospermic semen profiles were randomly selected. Semen analysis was done according to the WHO standard. Sex hormonal profiles were measured by ELISA methods. The level of vitamins (C and E) and MDA were measured by HPLC and tiobarbiotic acid, respectively. Results: MDA concentration in the spermatozoa (0.10.06 nmol/ml) and seminal plasma (1.90.35 nmol/ml) of asthenoteratozoospermic were significantly higher than in normozoospermic males (p<0.001).The level of vitamins E and C in seminal plasma of normozoospermic were significantly higher than in asthenoteratozoospermic males (p<0.01). However, the amount of vitamin C in the spermatozoa of normozoospermic was significantly less than in asthenoteratozoospermic males (p<0.01). Sensitivity, specificity, positive and negative prognostic value of MDA of seminal plasma and spermatozoa were more than vitamins C and E. The level of vitamin C of spermatozoa had more diagnostic value when compare to vitamins C and E in seminal plasma. Conclusion: The level of MDA in seminal plasma and spermatozoa and vitamin C in spermatozoa may be a diagnostic tool for etiology of infertility in the asthenoteratozoospermic patients.
Arash Khaki, Fatemeh Fathiazad, Mohammad Nouri, Amir Afshin Khaki, Chelar C Ozanci, Marefat Ghafari-Novin, Mohammad Hamadeh,
Volume 7, Issue 2 (7-2009)

Background: Ginger rhizome (Zingiber officinale R., family: Zingiberaceae) is used medicinally and as a culinary spice. Objective: Medicinal use of ginger dates back to ancient China and India. Ginger and its constituents are stated to have antiemetic, antithrombotic, antihepatotoxic, anti-inflammatory, stimulant, cholagogue and antioxidant. It has been used since ancient time as medicinal and food origins it contain antioxidative and androgenic activities and have well effect in diseases treatment in more countries world-wide. As an antioxidant’s ginger has a useful effect on spermatogenesis and sperm parameters. Materials and Methods: Wistar male rat (n=30) were allocated into three groups, control (n=10) and test groups (n=20), that subdivided into groups of 2 that received ginger rhizome powder (50 and 100mg/kg/day) for 20 consequence day. Animals were kept in standard conditions. In twentieth day the testes tissue of Rats in whole groups were removed and sperm was collected from epididymis and prepared for analysis. Results: Serum total testosterones significantly increased in experimental group that has received 100 mg/kg/day Ginger (p<0.05) in comparison to control group. Besides, the percentage of sperm viability and motility in both test groups significantly increased (p<0.05) in comparison to control group, Whereas, LH, FSH hormones, sperm concentration, morphology and testes weights in both experimental and control group were similar. Conclusion: Results revealed that administration of 100 mg/kg/day of ginger significantly increased sperm percentage, viability, motility and serum total testosterones. This suggested that ginger may be promising in enhancing sperm healthy parameters.
Korosh Khanaki, Ali Motavalizadeh Ardekani, Alieh Ghassemzadeh, Vahideh Shahnazi, Mohammad Reza Sadeghi, Masoud Darabi, Amir Mehdizadeh, Abotaleb Saremi, Jafar Soleimani-Rad, Ali Reza Imani, Mohammad Nouri, Ali Rahimipour,
Volume 10, Issue 4 (8-2012)

Background: Endometriosis is a common chronic inflammation which leads to infertility and chronic pelvic pain in affected women. Secretory phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is markedly increased in inflammatory disorders. Objective: To assess the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) administration in endometrial cells culture on sPLA2IIa level and cell survival comparing homolog ectopic versus eutopic endometrial cells from endometriosis patients. Materials and Methods: In this experimental study, ectopic and eutopic endometrial tissue samples obtained from 15 endometriosis patients were immediately frozen. After thawing and tissue digestion, mixed stromal and endometrial gland cells were cultured for 8 days in three different culture media; balanced ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell survival was measured using 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H- tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with ELISA technique. Results: The sPLA2IIa level was significantly higher in the ectopic endometrial cell culture compared to the eutopic group for each of the three matched treatments (balanced, high ω-3 and high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell group was remarkably increased by each of the three PUFAs treatments compared to control condition (p<0.05, p<0.01, p<0.05 respectively). Cell survival in the eutopic group was significantly decreased by high ω-6 culturing compared to control medium (p<0.05). Conclusion: The increase in sPLA2IIa level in ectopic endometrial cells by fatty acid treatments (especially high ω-3), strengthens the hypothesis that PUFAs stimulate secretion of cytokines leading to increased sPLA2IIa level.
Alireza Abhari, Nosratollah Zarghami, Laya Farzadi, Mohammad Nouri, Vahideh Shahnazi,
Volume 12, Issue 10 (11-2014)

Background: MicroRNA (miRNA) is small endogenous, single strand RNA molecules that regulate gene expression at post-transcriptional level through several mechanisms to affect key cellular event including male germ cells differentiation, proliferation, development and apoptosis. Mutation and/or aberrant expression of miRNAs have been associated with progression of various disorders, including infertility.
Objective: The purpose of this research was to study the estrogen receptor beta (ERβ), hsa-mir-21 and, hsa-mir-22 expression level in oligospermic infertile and control fertile men and correlation between them.
Materials and Methods: In this study, the change in mir-21, mir-22 expression and their common target gene (ERβ) expression levels were evaluated in oligospermic infertile men (n= 43) compared with 43 age matched healthy control by Real-Time PCR methods.
Results: Expression analysis by qRT-PCR test on miRNA have identified that mir-21, mir-22 levels were significantly higher than those in normal controls (p<0.0001) and ERβ expression level significantly decreased in comparison with the normal group (p<0.0001).
Conclusion: Our study showed that mir-21 and mir-22 are indirectly involved in spermatogenesis by regulating of the estrogen receptor and might have a diagnostic and prognostic value in men infertility.
Korosh Khanaki, Mohammad Reza Sadeghi, Mohammad Mehdi Akhondi, Masoud Darabi, Amir Mehdizadeh, Mahdi Shabani, Ali Rahimipour, Mohammad Nouri,
Volume 12, Issue 11 (12-2014)

Background: Endometriosis, a common chronic inflammatory disorder, is defined by the atypical growth of endometrium- like tissue outside of the uterus. Secretory phospholipase A2 group IIa (sPLA2-IIa) and fatty acid binding protein4 (FABP4) play several important roles in the inflammatory diseases.
Objective: Due to reported potential anti-inflammatory effects of ω-3 and ω-6 fatty acids, the purpose of the present study was to investigate the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) on fatty acid binding protein 4 and extracellular secretory phospholipase A2IIa in cultured endometrial cells.
Materials and Methods: Ectopic and eutopic endometrial tissues obtained from 15 women were snap frozen. After thawing and tissue digestion, primary mixed stromal and endometrial epithelial cell culture was performed for 8 days in culture mediums supplemented with normal and high ratios of ω-3 and ω-6 PUFA. sPLA2-IIa in the culture medium and FABP4 level was determined using enzyme immuno assay (EIA) technique.
Results: Within ectopic endometrial cells group, the level of cellular FABP4 and extracellular sPLA2-IIa were remarkably increased under high ω-3 PUFA exposure compared with control condition (p=0.014 and p=0.04 respectively).
Conclusion: ω-3 PUFAs may increase the level of cellular FABP4 and extracellular sPLA2-IIa in ectopic endometrial cells, since sPLAIIa and FABP4 may affect endometriosis via several mechanisms, more relevant studies are encouraged to know the potential effect of increased cellular FABP4 and extracellular sPLA2-IIa on endometriosis.
Vahideh Shahnazi, Mina Zaree, Mohammad Nouri, Mahzad Mehrzad-Sadaghiani, Shabnam Fayezi, Maryam Darabi, Sajjad Khani, Masoud Darabi,
Volume 13, Issue 2 (2-2015)

Background: The omega-3 (ω-3) fatty acid eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement to improve infertility, particularly in women with polycystic ovarian syndrome (PCOS).
Objective: The present study was designed to investigate the effect of EPA on insulin-like growth factor 1 (IGF-1) and cyclooxygenase 2 (COX-2) gene expression in primary cultured granulosa cells from patients undergoing in vitro fertilization (IVF), and also to compare this effect with those in granulosa cells of PCOS patients.
Materials and Methods: In this experimental study, human granulosa cells were isolated from follicular fluid of normal and PCOS women undergoing IVF by hyaluronidase digestions, followed by Percoll gradient centrifugation. Cells were cultured in vitro, exposed to a range of concentrations of the EPA (25-100 μM) for 24 hr, and investigated with respect to COX-2 and IGF-1 gene expression by real time-PCR.
Results: In both groups, all doses of the EPA significantly induced IGF-1 mRNAgene expression compared to the untreated control. High doses of EPA in thepresence of recombinant (r) FSH produced a stimulatory effect on IGF-1 and asuppressive effect (p=0.01) on the COX-2 gene expression, which were morepronounced in granulosa cells from PCOS patients.
Conclusion: EPA affect diversely the gene expression of IGF-1 and COX-2 in granulosa cells, which were more pronounced in PCOS compared to control. These findings represent the possible underlying molecular mechanisms for the positive impact of the ω-3 fatty acids on reproduction, especially in patients with PCOS.
Laya Farzadi, Homa Khayatzadeh Bidgoli, Morteza Ghojazadeh, Zahra Bahrami, Amir Fattahi, Zeinab Latifi, Vahideh Shahnazi, Mohammad Nouri,
Volume 13, Issue 6 (8-2015)

Background: Vitamin D in complex with its receptor by regulating gene expression, endometrium immune response and stimulation of endometrium decidualization can be involved in implantation. So, it seems that the amount of vitamin D in follicular fluids (FF) may have an association with ART success.
Objective: First, we intended to investigate the possible association between levels of follicular fluids 25-OH vitamin D with assisted reproductive outcomes. Second, we examined relationship between 25-OH vitamin D levels with number and quality of oocytes.
Materials and Methods: In a prospective study, 80 infertile female candidates for IVF/ICSI were enrolled. Blood samples (on the day of human chorionic gonadotropin administration) and follicular fluids were taken, and then levels of serum estradiol and follicular fluids 25-OH vitamin D were measured. Also clinical characteristics of patients (duration of infertility, causes of infertility, menstrual status), number and quality of oocytes, number of fertilized oocytes, estradiol levels, and clinical pregnancy were evaluated.
Results: Concentration of FF 25-OH vitamin D in pregnant women was significantly higher than non-pregnant women (p=0.007) but there were no significant differences in age, body mass index (BMI), duration of infertility, menstrual status, number of oocytes, oocytes quality, number of fertilized oocytes, and serum estradiol levels between the two groups. Statistically positive correlation was found between 25-OH vitamin D levels with patient age and implantation rate (r=0.264, p=0.018 and r=0.301, p=0.007 respectively).
Conclusion: The obtained results suggest that vitamin D without affecting the number and quality of oocytes can independently improve implantation rate and IVF outcome.
Zohreh Hojati, Fatemeh Nouri Emamzadeh, Fariba Dehghanian,
Volume 14, Issue 6 (6-2016)

Background: Some dynamic changes occurs during spermatogenesis such as histone removal and its replacement with transition nuclear protein and protamine. These proteins are required for packing and condensation of sperm chromatin. JHDM2A is a histone demethylase that directly binds to promoter regions of Tnp1 and Prm1 genes and controls their expression by removing H3K9 at their promoters.
Objective: The association between polymorphisms of exon 12 and exon 24 inJHDM2A gene and male infertility were evaluated for the first time.
Materials and Methods: In this experimental study, 400 infertile men (oligospermia and azoospermia) and normal healthy fathers were evaluated (n=200). Single Strand Conformation Polymorphism (SSCP-PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for screening any polymorphisms that are exist in exon 12 and exon 24.
Results: Exon 24 PCR products were analyzed by RFLP but no polymorphism was found in this exon at the restriction site of EcoRV enzyme. Our monitoring along the whole nucleotides of exon 12 and exon 24 were continued using SSCP method, but we found no change along these exons.
Conclusion: Generally, this study evaluated the association between polymorphisms in exon 12 and exon 24 of JHDM2A gene and male infertility which suggests that polymorphisms of these exons may not be associated with the risk of male infertility.
Najaf Zare, Bijan Nouri, Fariba Moradi, Maryam Parvareh,
Volume 15, Issue 1 (1-2017)

Background: Time to first pregnancy (TTFP) has never been studied in an Iranian setting. Lifestyle, occupational and environmental factors have been suggested to affect the female reproduction.
Objective: This study was conducted to measure TTFP in the south of Iran and survey the effects of several similar factors on TTFP by frailty models.
Materials and Methods: The data on TTFP were available for 882 women who were randomly selected from the rural population (the south of Iran). Only the first and the planned pregnancies of every woman were included. The data were collected retrospectively by using self-administered questionnaires. Frailty and shared frailty models were used to determine which factors had the highest impact on TTFP.
Results: The median TTFP was 6.4 months and several factors were surveyed. However, only the age of marriage, height, maternal education and regularity of menstruation prior to conception were selected in the multivariable models.
Conclusion: Among the several factors which were included in the study, the result of frailty model showed that the height, age of marriage and regular menstruation seemed more notable predictors of TTFP.

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