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Showing 5 results for Nikzad

Homayoun Naderian, Hossein Nikzad, Akbar Aliasgharzadeh, Mohammad Ali Atlasi,
Volume 7, Issue 2 (7-2009)
Abstract

Background: Polycystic ovarian syndrome (PCOS) is one of the most common endocrine disorders which cause anovulatory infertility and hyperandrogenism in young women. The common feature in PCOS women is increased ovarian androgen secretion which can effect on the prevalence of miscarriage rate.
Objective: The aim of this study was to investigate the effect of PCOS patient's serum on in vitro developmental stages of mouse embryo from two cells to hatching blastocyst.
Materials and Methods: After superovulating and fertilizing Balb/c mice, 219 two cells embryos were retrieved, 109 embryos were cultured in 10% PCOS patient's serum and 90% medium and 110 embryos were cultured in 10% normal serum and 90% medium to hatching blastocyst stage. The PCOS patient's serum which added to medium had higher hormonal concentrations than normal serum. The early developmental stages of embryos were studied in 2, 4, 8 cells, morula, early, late and hatching blastocyst stages.
Results: The statistical analysis confirmed the decreasing rate in the number of embryos in all developmental stages from 2 cells to hatching blastocyst in PCOS group in comparison with the normal group (p<0.05).
Conclusion: The PCOS patient's serum causes the decreasing rate of in vitro development of the early stage in mouse embryos.
Hossein Nikzad, Maryam Kabir-Salmani, Shigetatsu Shiokawa, Yoshiro Akimoto, Mitsutoshi Iwashita,
Volume 8, Issue 5 (7-2010)
Abstract

Background: Pinopodes are suggested as biological markers of uterine receptivity, but their molecular components are unknown.
Objective: Co-expression of galectin-3 and avb3 integrin at human pinopodes has been examined in this study to propose a role for them during adhesion phase of embryo implantation.
Materials and Methods: Biopsies were obtained from early and mid luteal phase endometrium of 12 fertile women with regular menstrual periods (25-35 days) and the mean age of 37 years (range 25–45). Then, they were examined under light and scanning electron microscopy for detection and dating of pinopodes. Using immunofluorescent staining and immunogold electron microscopy, the expression of galectin-3 and avb3 integrin in human endometrium and pinopodes was detected. Further, statistical analysis was performed using immunogold electron microscopy to investigate the expression and subcellular distribution of these, before and during the frame of implantation window.
Results: The results demonstrated that pinopodes of luminal epithelial cells exhibited immunoreactivity for both galectin-3 and αvβ3 integrin, which was increased statistically significant (p< 0.05) at the time of implantation window. Furthermore, area-related distribution of these proteins was found higher in pinopodes compared to the neighboring apical membrane without pinopode.
Conclusion: Temporal and spatial expression of galectin-3 and αvβ3 integrin at pinopodes proposes a role for pinopodes in the adhesion of embryo and the involvement of galectin-3 as a binding partner of integrins in the human utero-fetal complex.
Hossein Nikzad, Hamed Haddad Kashani, Maryam Kabir-Salmani, Yoshihiro Akimoto, Mitsutoshi Iwashita,
Volume 11, Issue 1 (4-2013)
Abstract

Background: The up-regulation of galectin-3, galectin-9, and galectin-15 expression in the luminal and glandular epithelium was reported in preparation of the endometrium for embryo implantation at the midlutheal phase. However, no data was available regarding the expression and the distribution pattern of galectin-8 in the human endometrium during a regular menstrual cycle.
Objective: The current study designed to investigate the expression and the distribution pattern of galectin-8, a beta-galactoside-binding lectin in the human endometrium during both proliferative and luteal phases of a regular menstrual cycle.
Materials and Methods: Endometrial biopsies were obtained from the anterior wall of the uterine cavity of 16 women (proliferative phase: n=4, lutheal phase: n=12). All female patients with mean age of 37.5 years were fertile (range 25-45) . Each biopsy was divided into three pieces; one piece was fixed in formaldehyde for light microscopy and immunohistochemistry. The second portion fixed in glutaraldehyde for scanning electron microscopy and the third portion was prepared for western blot analysis.
Results: Data of immunoblotting revealed a molecular weight of 34 kD band with high intensity in the lutheal phase samples. The immunohistochemistry staining demonstrated that galectin-8 expressed at a very low concentration during the proliferative phase, but showed a high expression throughout the lutheal phase. The expression of galectin-8 observed in luminal surface epithelium, glandular epithelium and stroma.
Conclusion: The up-regulation of the expression of galectin-8 during lutheal phase may suggest galectin-8 as one of the potential molecular marker of the endometrial receptivity. These data propose that galectin-8 may play an important role during the initial events of human embryo implantation.
Leila Roshangar, Jafar Soleimani-Rad, Bahman Rashidi, Hossein Mazochian, Behzad Nikzad, Sara Soleimani Rad,
Volume 11, Issue 10 (12-2013)
Abstract

Background: Endometrial development has an important role in blastocyst adhesion and implantation. During IVF cycles, endometrial development is enhanced by progesterone.
Objective: The aim of this study was to compare ultrastructural and morphometrical characteristics of mice uterine endometrium in natural cycle with those in superovulated cycles received progesterone or Sildenafil.
Materials and Methods: In This study, 60 female bulb/c mice were divided into 4 groups: a control and 3 experimental; gonadotropin, gonadotropin+ Sildenafil and gonadotropin+ progesterone. In experimental groups the mice superovulated mated. In the gonadotropin+ progesterone and gonadotropin+ Viagra groups, the mice respectively received 1mg progesterone and 3 mg Sildenafil citrate. Their uterine specimens were prepared for morphometrical and ultrastructural study. Height of the epithelial cells was measured, using motic software. Statistical analysis was performed using ANOVA.
Results: Microscopy revealed that in control group the cells had numerous apical microvilli and the height of the cells was 20.52±2.43 μm. In gonadotropin+ progesterone group, the granules were found in basal and apical portions and cellular height were 17.91±2.78 μm which were significantly shorter than in the control and gonadotropin groups (p<0.001). In this group, the apical membrane also contained pinopodes. In gonadotropin +Sildenafil group, the granules were found in both apical and basal portions and the height of the cells were 17.60±2.49 μm which were significantly shorter than in the control and gonadotropin groups (p<0.001). In this group, pinopodes appeared slightly extensive than the other groups.
Conclusion: It is concluded that superovulatory drugs in mice stimulate endometrial maturation but injection of Sildenafil is nearly more positive.
Zohreh Khodayari Naeini, Hassan Hassani Bafrani, Hossein Nikzad,
Volume 12, Issue 4 (5-2014)
Abstract

Background: An effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells.
Objective: This study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men with normal semen parameters.
Materials and Methods: Semen samples (n=35) were collected by masturbation and assessed following WHO standards. Individual samples were classified as two portions. One portion (n=10) was for elucidate the concentration of ebselen.Then the samples(n=25) were divided in to 5groups.The first aliquot remained fresh.The second aliquots was mixed with cryopreservation medium.The third aliquots were mixed with cryopreservation medium containing solvent of ebselen.The forth and fifth aliquots were mixed with cryopreservation medium containing 1.25 and 2.5 µm of ebselen.Samples were frozen and thawed samples were assessed for sperm parameters.Three-way ANOVA Multivariate measures were used to assess. According to this assesment the differences are observed in existent groups in post-thaw count, motility index, vitality staining, and morphology and DNA fragmentation.
Results: After freezing the media containing of ebselen, DNA fragmentation is significantly different in comparison with control group. ebselen with 1.25 µm dose was significantly associated with post-thaw DNA fragmentation (p=0.047). Similarly ebselen with 2.5 µm dose was significantly associated with post-thaw DNA fragmentation (p=0.038). But other parameters were not altered.
Conclusion: These results suggest that the addition of ebselen to cryopreservation medium doesnot improve post-thaw parameters and DNA fragmentation of sperm

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