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Showing 4 results for Nasr-Esfahani

Mohamad Reza Darabi, Mohamad Hosein Nasr-Esfahani, Hosein Baharvand, Mohmad Mardani, Hojatolah Karimi-Jashni,
Volume 6, Issue 5 (7-2008)

Background: The values of embryonic stem cell and cloning are evident. Production of clone from embryonic stem cells can be achieved by introduction of stem cell into a tetraploid blastocyst. Tetraploid blastocyst can be produced in vitro by electrofusion of 2-cell embryos.
Objective: The aim of this study was to assess the effect of different voltages and durations on fusion rate of bovine 2-cell embryos and their subsequent development in vitro.   
Material and Methods: The in vitro produced bovine 2-cell embryos were categorized into 3 groups: (1) fused group (FG); 2-cell embryos fused by exposure to different voltages (0.5, 0.75, 1, 1.25 and 1.5 kV/cm) and durations (20, 40, 60, 80 and 100 μs), (2) exposed control group (ECG);  2-cell embryos exposed to different voltages and durations but remained unfused and (3) unexposed control group (UCG); embryos cultured without exposure to any voltage. The embryos from each group were cultured and fusion, cleavage and developmental rates were compared in each group.
Results: The results show that increased voltage, increases the fusion rate up to 88% for 1.5 kV/cm; however, the rate of cleavage and blastocyst formation decreases significantly to 18% and 10% respectively (p<0.05). Increased duration does not significantly increase fusion rate, however, in high voltage, increased duration decreases cleavage rate and blastocyst formation rate. Blastocyst formation rate in UCG showed a better development (32%) compared to FG (20%) or ECG (22.5%) (p<0.05).
Conclusion: It can be concluded that for optimal fusion, cleavage and development, one pulse of 0.75 kV/cm for 60μs should be applied.
Noush Afarin Khajavi, Shahnaz Razavi, Mohammad Mardani, Marziyeh Tavalaee, Mohammad Reza Deemeh, Mohammad Hossein Nasr-Esfahani,
Volume 7, Issue 2 (7-2009)

Background: Sperm selection for ICSI based on morphology and motility might not be relevant to chromatin integrity. Thus sperm selection based on sperm characteristics has been suggested.
Objective: The aim of this study was to compare the efficiency of Zeta method with routine Density Gradient Centrifugation method (DGC) for the selection of sperm with higher DNA integrity.
Materials and Methods: Semen samples were obtained from 63 individuals referring to Andrology Unit of Isfahan Fertility and Infertility Center. Semen analysis was carried out according to WHO criteria. Each semen sample was divided into three equal portions. One portion was used as control, the second portion was used for Zeta method and the third portion underwent DGC method. Each portion was evaluated to DNA integrity by TUNEL assay. Student t-test was carried out using SPSS and p-value lower than 0.05 was considered significant.
Results: The mean number of sperm DNA fragmentation in Zeta and DGC methods were significantly decreased compare to the control group (p<0.001). In addition, Zeta method was more efficient than the DGC method in the selection of sperm with intact DNA (p<0.001).
Conclusion: The Zeta method appears to be a suitable procedure to recover sperm with normal DNA integrity.
Ashraf Kazemi, Fatemeh Ramezanzadeh, Mohammad Hosein Nasr-Esfahani, Ali Akbar Saboor Yaraghi, Mehdi Ahmadi,
Volume 11, Issue 12 (1-2013)

Background: Fat-rich diet may alter oocyte development and maturation and embryonic development by inducing oxidative stress (OS) in follicular environment.
Objective: To investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality.
Materials and Methods: In observational study follicular fluid was collected from 236 women undergoing assisted reproduction program. Malon-di-aldehyde (MDA) levels and total antioxidant capacity (TAC) levels of follicular fluid were assessed as oxidative stress biomarkers. In assisted reproduction treatment cycle fat consumption and its component were assessed. A percentage of metaphase ΙΙ stage oocytes, fertilization rate were considered as markers of oocyte competence and non-fragmented embryo rate, mean of blastomer and good cleavage (embryos with more than 5 cells on 3 days post insemination) rate were considered as markers of embryo quality.
Results: The MDA level in follicular fluid was positively related to polyunsaturated fatty acids intake level (p=0.02) and negatively associated with good cleavage rate (p=0.045). Also good cleavage rate (p=0.005) and mean of blastomer (p=0.006) was negatively associated with polyunsaturated fatty acids intake levels. The percentage of metaphase ΙΙ stage oocyte was positively related to the TAC levels in follicular fluid (p=0.046). The relationship between the OS biomarkers in FF and the fertilization rate was not significant.
Conclusion: These findings revealed that fat rich diet may induce the OS in oocyte environment and negatively influence embryonic development. This effect can partially be accounted by polyunsaturated fatty acids uptake while oocyte maturation is related to TAC and oocytes with low total antioxidant capacity have lower chance for fertilization and further development.
Ashraf Kazemi, Fatemeh Ramezanzadeh, Mohammad Hosein Nasr-Esfahani,
Volume 13, Issue 9 (10-2015)

Background: Oxidative stress (OS) in the follicular environment may affect on oocyte competence and antioxidant vitamins may modify its effects.
Objective: This study was conducted to examine the effect of dietary intake of vitamin A, C and E on OS in follicular environment and assisted reproduction technology (ART) outcomes.
Materials and Methods: In this obsevationalprospective study, the intake levels of vitamin A, C, and E were matured by validated food frequency questionnaire and Malondialdehyde and the total antioxidant capacity (TAC) levels of follicular fluid (FF) in 219 women undergoing ART were assessed. The number of retrieved oocytes, percentages of metaphase II MII) stage oocytes, fertilization rate, and embryo quality were also determined.
Results: No significant association was found between vitamins intake levels and OS biomarkers, but the mean of TAC level in FF among women who received vitamin C greater than 75 mg/d was higher than women with lower intakes (p<0.05). The ART parameters were not related to the vitamin E intake level, but the normal cleaved embryo rate was positively related to vitamin A (p<0.05) and vitamin C (p=0.02) intake levels. Also, the percentage of MII oocytes (p=0.02) and the fertilization rate (p<0.05) were related to the vitamin C intake level. The relation between the TAC level in FF and ART outcomes were not significant.
Conclusion: Current results indicated that high dietary intake of vitamin C would be followed by increasing the TAC level in FF and improving the oocyte competence, but this effect of vitamin C is not dependent of increasing of antioxidant defense in follicular environment.

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