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Showing 7 results for Mazaheri

Soghra Bahmanpour, Mohammad Reza Namavar, Tahereh Talaei, Zohreh Mazaheri, Ahmad Monabati,
Volume 7, Issue 3 (7-2009)

Background: Some evidences showed that the secretion of uterine tube vagina and follicular fluid (FF) affects X and Y-chromosome populations. Sperm selection with X or Y chromosome can added to oocyte for gender desired. The isolation of X Y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. The objective of this study was to find if the FF can influence the ratio of X or Y chromosomes therefore we added human FF to culture media to separate X and Y sperms.
Materials and Methods: Normal semen sample from 36 healthy men were selected. Then the samples were divided into control and experimental groups: control group sperms have been incubated with conventional culture media (Ham,s F-10) and experimental group with conventional culture media + 10% human FF. For sperm isolation swim up technique was used. After 24 hours of incubation slides smear were prepared. Then we used the Fluorescent in Situ Hybridization (FISH) method to evaluate the effect of follicular fluid on the population ratio of X and Y containing sperms.
Results: Although the incubation of sperm in FF and Ham,s F-10 increased Y sperm (59.44 % in control and 61.42% in experimental groups) in comparison with X sperm (40.56% in control and 38.5 in experimental groups) significantly (p<0.05), but the Y (or X) bearing sperm did not significantly change in experimental group in comparison with Y (or X) bearing sperm in control group.
Conclusion: This study showed that using the swim up method for collecting sperms and adding FF to culture media can improve some sperm parameters, but did not has significant effects on population of X and Y sperm.
Mahsa Askari Jahromi, Mansoureh Movahedin, Zohreh Mazaheri, Masoud Amanlu, Seyed Javad Mowla, Hosein Batooli,
Volume 12, Issue 7 (8-2014)

Background: Catsper proteins are responsible for entering Ca2+ to the cell and play an important role in sperm motility and male fertility. Antioxidants are vital for sperm motility too. Escanbil (Calligonum) extract possess some of the important antioxidant like Catechin and Quercetin.
Objective: Here we investigated the effects of Escanbil (Calligonum) extract on the sperm parameters and the expressing of Catsper gene in aging male mice.
Materials and Methods: In this animal study, firstly, dose response was performed by using these three doses of Escanbil (Calligonum) (10, 30 and 50 mg/kg). 5 mice in each group were considered and Intra Peritoneal injection was done for 5 weeks. the sperm parameters analyzed and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL )staining was done. 30 mg/kg dose was considered as optimum dose. Secondly: fifteen aging male mice (11-13 months) were divided into three groups: control, sham and experiment. The experiments were injected Intra peritonealy with Escanbil (Calligonum) extract (30mg/kg) weekly for up to 5 weeks. The sham group was injected Intra Peritoneal (DMSO). Sperm parameters were analyzed. Expression of Catsper genes was analyzed by Real time PCR.
Results: Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05). Our data showed that there was a statistical significance difference between the expressions of Catsper 2, 4 in aging experiment group comparison with aging control group (p<0.05).
Conclusion: We investigated that the Escanbil (Calligonum) extract (30 mg/kg) can improve sperm parameters and change the expression of Catsper genes in aging male mice. This herbal extract can be used as an antioxidant component for clinical usages.
Fatemeh Peyghambari, Saeid Amanpour, Mehri Fayazi, Mahnaz Haddadi, Samad Muhammadnejad, Ahad Muhammadnejad, Mehdi Salimi, Zohreh Mazaheri,
Volume 12, Issue 9 (10-2014)

Background: It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes (PCOS).
Objective: In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model.
Materials and Methods: 30 NMRI female mice were equally divided into 3 groups: control, experimental [PCOS that was injected estradiol valerate (40 mg/kg)]. After 8 weeks, each group was hyper stimulated by PMSG and HCG. Vaginal plaque was checked, and mice were investigated 5 days after the test. Progesterone and estradiol levels were determined; α4, αv, β1 and β3 integrin genes and protein of blastocysts were examined by real time PCR method and immunohistochemistry, respectively.
Results: Estradiol level was significantly increased (p≤0.035) in PCOS group. Based on our finding, the ratio of genes' expressions αv, β3, β1 and α4 in PCOS to control group was 0.479±0.01, 0.5±0.001, 2.7±0.4 and 1.023±0.2 respectively. Genes expression showed a great difference (p≤0.001) between β3, β1 and αv in PCOS compared to other groups. αv and β3 integrin proteins expressed in all groups but intensity of these proteins in PCOS groups, was lower than other groups.
Conclusion: Pattern of αv and β3 integrins expression on the mouse blastocyst surface has an important effect during the implantation window. This pattern has changed in PCOS model and might have a great influence on implantation failure. Therefore, this experimental study suggests that a great attention to this problem may be essential in patients who are involved.
Fatemeh Peyghambari, Mehri Fayazi, Saeid Amanpour, Mahnaz Haddadi, Samad Muhammadnejad, Ahad Muhammadnejad, Samira Abdolahpour, Mozhgan Enkesari, Zohreh Mazaheri,
Volume 12, Issue 10 (11-2014)

Background: Endometrial integrin expression changes might be a reason for implantation failure in polycystic ovarian syndromes (PCOS).
Objective: Assessment of integrin genes and proteins expression upon endometrium in the PCOS experimental mouse model was the main goal of this study.
Materials and Methods: 30 NMRI female mice were equally divided into control, experimental (PCOS; received estradiol valerate (40 mg/kg)) and sham group (received; olive oil). After 8 weeks, each group was hyper stimulated by 7 IU PMSG and then, after 48hrs, 7 IU HCG was injected. Vaginal plaque was checked. After 5 days, Progesterone and estradiol levels and endometrial tissues were investigated to evaluate of α4, αv, β1 and β3 integrins gene and protein by qPCR method and immunohistochemistry, respectively.
Results: Tissue samples were assessed and showed that level of progesterone was significantly decreased in PCOS group. Results of molecular part in the amount of αv, β3, β1 and α4 gene expressions showed a great difference in β3 and αv genes expressions between experimental groups. αv, β3, α4 and β1 proteins in the endometrial stroma in the control group were expressed, but they were not detected in PCOS group.
Conclusion: According to the results, integrins had different expression patterns in different areas of the endometrium; such as epithelial and stromal. It seems that in PCOS, this pattern has changed and the results might have a great influence on implantation failure. Therefore, this study suggests that a great attention to this problem may be essential in patients who are involved.
Mahta Mazaheri, Vahid Shahdadi, Ashraf Nazari Boron,
Volume 12, Issue 11 (12-2014)

Background: Alpinia galanga (A. galanga) belongs to the Zingiberaceae family has anti-oxidant effects in animals and humans body and often is used as medicament or part of medicaments in Asian folk medicine for various applications.
Objective: The objective of this study was to investigate the molecular and biochemical influence of alcoholic extract from the rhizomes of A. galangal on the spermatogenesis process in male rat.
Materials and Methods: Forty five Wistar male rats were divided into three groups, control (n=15) and two tested groups (n=30). Alcoholic extract (5%) of plant was given by oral route at doses of 100 and 300 mg/day for 56 days and spermatogenesis parameters, hormone changes and expression level of the cAMP-responsive element modulator (CREM) gene were assessed.
Results: Methanol extract of A. galanga increased serum testosterones level significantly in both treated groups in comparison with control group (p<0.05). Besides, the percentage of sperm viability and motility in both tested groups were significantly increased. Follicle stimulating hormone FSH hormone, morphology and weight were affected in both treated groups. With 300 mg/day an increase in sperm count was observed. Sperm motility was increased in two treated groups whereas testis weight was decreased in treated groups. Real time analysis of treated cells of testis showed increase level of mRNA related to CREM gene involved in spermatogenesis process after 56 days induction.
Conclusion: It is concluded that application of ethanolic extract of A. galanga significantly increased sperm percentage, viability, motility and testosterone hormone. This suggested that this plant may be promising in enhancing sperm healthy parameters.
Nasrin Majidi Gharenaz, Mansoureh Movahedin, Zohreh Mazaheri, Shahram Pour Beiranvand,
Volume 14, Issue 8 (8-2016)

Background: Nowadays, oocytes and embryos vitrification has become a routine technique. Based on clinical judgment, re-vitrification maybe required. But little is known about re-vitrification impact on genes expression.
Objective: The impact of re-vitrification on apoptotic and implanting genes, Bax, Bcl-2 and ErbB4, at compaction stage embryos were evaluated in this study.
Materials and Methods: In this experimental study, 8 cell embryos (n=240) were collected from female mature mice, 60-62 hr post HCG injection. The embryos were divided randomly to 3 groups included: fresh (n=80), vitrified at 8 cell stage (n=80), vitrified at 8 cell stage thawed and re-vitrified at compaction stage (n=80). Embryos were vitrified by using cryolock, (open system) described by Kuwayama. Q-PCR was used to examine the expression of Bax, Bcl2 ErbB4 genes in derived blastocysts.
Results: Our result showed that expanded blastocyst rate was similar between vitrified and re-vitrified groups, while re-vitrified embryos showed significant decrease in expanded blastocyst rate comparing with fresh embryos (p=0.03). In addition, significant difference was observed on apoptotic gene expression when comparing re-vitrified and fresh embryos (p=0.004), however expression of Bax and Bcl-2 (apoptotic) genes didn't demonstrate a significant difference between re-vitrified and vitrified groups. The expression rate of ErbB4, an implantation gene was decreased in re-vitrified embryos comparing with fresh embryos (p=0.003), but it was similar between re-vitrified and vitrified embryos.
Conclusion: Re-vitrification can alter the expression of Bax, Bcl-2 and ErbB4 genes and developmental rate of mouse embryos in compaction stage.
Fatemeh Tahmasebi, Mansoureh Movahedin, Zohreh Mazaheri,
Volume 16, Issue 10 (October 2018)

Background: Polycystic ovary syndrome (PCO) is one of the most common reasons for infertility. Calligonum as a plant possess some of the important antioxidants that can decrease oxidative stress.
Objective: The effects of treatment with Calligonum as an antioxidant on ovary tissue of a PCO mouse model.
Materials and Methods: Thirty female NMRI mice were divided into three groups (n=10/each): control, PCO, and Calligonum. We induced PCO model with single dose of Estradiol valerate (40 mg/kg). Then Calligonum (20 mg/kg) was intraperitoneally injected weekly for two months. The level of oxidative stress and total antioxidant capacity was assessed in the ovarian tissue by flow cytometry and fluorescence recovery after photobleaching, respectively, and the histological study was conducted by the morphometric method and embryo development with in vitro fertilization.
Results: The obtained results showed that estradiol valerate was able to increase oxidative stress within the ovary and causes ovarian cysts after two months. The cyst formation was decreased in Calligonum group compared to PCO group (p=0.001). The percentage of pre-antral and antral follicles significantly decreased in Calligonum group compared to PCO group (p=0.001). The oxidative stress decreased in Calligonum group significantly compared to PCO group (p=0.001). Calligonum can significantly increase the total antioxidant capacity of ovarian tissue (p=0.001) as well as the percentage of in vitro fertilization compared to the PCO group.
Conclusion: Calligonum could decrease ovary cyst in PCO model, and improve in vitro fertilization rate. Also, Calligonum extract as an antioxidant could decrease oxidative stress in PCO model.

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