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Showing 20 results for Heidari

Soheila Arefi, Mahmoud Jeddi Tehrani, Mohammad Mehdi Akhondi, Ali Reza Mousavi, Mahnaz Heidari, Ahmad Ali Bayat, Ali Sadeghpour Tabaei,
Volume 3, Issue 1 (7-2005)
Abstract

Background: Auto antibodies to zona-pellucida (AZA) seem to be important autoantibodies implicated in reproduction, with substantial role in both endocrine and reproductive functions of the human ovary. There are some debates on the relation of AZA with infertility, repeated In Vitro Fertilization (IVF) attempts, and outcome of it. Objective: In this study, we assessed the presence of AZA in the follicular fluids (FFs) of women who underwent intra cytoplasmic sperm injection (ICSI), in relation to etiology of infertility and multiple puncture of ovaries. Materials and Methods: In this prospective study, follicular fluids were evaluated from 96 infertile women, (19-40 years old, 31.5�5.1), who were candidates for ICSI based on the etiology of infertility. From these 80 women had explained infertility whereas 16 had unexplained infertility. All FFs were evaluated for presence of AZA by ELISA test. Results: Twenty patients (20.8%) were positive for AZA in follicular fluid. In patients with unexplained infertility, AZA antibody in follicular fluid, was significantly higher than the group with proven etiology of infertility (p=0.001). In addition, 20.4 % of patients who had been punctured previously showed AZA in their FFs which is statistically similar to the patients who were punctured for the first time. Conclusions: The high incidence of AZA in infertile women, especially women with unexplained infertility has to be considered. Relation of the presence AZA and repeated puncture of ovaries is still debatable. Determinations of AZA are highly recommended in evaluation of infertile couples especially in patient with unexplained infertility
Afsaneh Mohammadzadeh, Mahnaz Heidari, Haleh Soltan Ghoraii, Amir Hassan Zarnani, Marefat Ghaffari Novin, Mohammad Mahdi Akhondi, Alireza Mossavie Jarahi, Farzaneh Mohammadzadeh,
Volume 4, Issue 2 (7-2006)
Abstract

Background: Endometriosis is defined as the growth of endometrial tissues in ectopic places outside the uterus. This disease has an important effect on the health and fertility of affected women. It’s etiology is not clearly known. For better understanding the pathophysiology of this disease, many researchers study on several aspects of the disease on animals.
Objective: In this experimental study endometriosis was induced in female rats surgically and then its side effects were investigated with special focus on adhesion formation that is a major problem in women with this disease.
Materials and methods: In Protestrous phase, female rats were randomly divided into two groups. In both groups, under intra peritoneal anesthesia, laparotomy was done and left horn and associated fat were removed. In experimented group (A), the removed endometrium was cut to six square pieces (2mm each) and they were sutured to the peritoneum, near ovaries and subcutaneous. In sham group (B), the same procedure was done for the fat tissues around the removed horn and the pieces were sutured to the same places. After 8 weeks, in Protestrous phase, clinical adhesion and size of implants were evaluated.
Results: The total mean size of implants was calculated in each group, and this was significantly larger in experimented group (25.4 mm versus 2 mm p=0.000). The mean diameter of implants that calculated for each site of implantation in experimented group were significantly larger in left peritoneum (p=0.002), followed by right (p=0.000) and left (p=0.000) ovaries. The endometrial tissues grew in 100% of implants in subcutaneous area. Clinical adhesions (Score ≥ 2) were detected in 7 out of 10 in experimented group and in 2 out 10 in control group. The number of Esterous cycle were similar in both groups.
Conclusion: Our study showed that after inducing endometriosis by surgical approach, only endometrial implants grew as a cystic structures and this is a unique aspect of endometrial cells. Our results showed that endometriosis had a direct effect on adhesion formation, not surgery alone and induction of this disease didn't have any adverse effect on ovarian function in female rats.
Marefat Ghaffari Novin, Mahnaz Heidari, Mahdi A Akhondi, Mahmood Jeddi Tehrani,
Volume 5, Issue 4 (7-2007)
Abstract

Background: Matrigel (extracellular matrix) can improve the growth of many cell types in vitro.
Objective: The aim of the present study was to determine the effect of Matrigel on the development of 2-4 cells human embryos in culture.
Material and Methods: Surplus 2-4 cells human embryos, resulting from ICSI, were divided into two groups (control and test). Quality of embryos in both groups was morphologically similar. The test group (n=140) was cultured in Hams’ F10 supplemented with 10% human serum albumin and 150 µl liquid Matrigel. The control group (n=140) was cultured in the same medium devoid of Matrigel. Embryos were cultured for an additional 4 days and their morphology was assessed every 24 hours. Both groups were then statistically compared.
Results: The percentage of the human embryos