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Showing 3 results for Golshan Iranpour

Farhad Golshan Iranpour, Mojtaba Rezazadeh Valojerdi,
Volume 11, Issue 3 (5-2013)
Abstract

Background: When male animals die, spermatozoa within the body of animal will be degenerated. Because of unique chromatin structure of sperm, maybe this degeneration is different from other cells. However there is not any research which considered directly the integrity of sperm DNA by keeping the cadaver in refrigerator.
Objective: The aim of this study was to assess viability, total motility and DNA integrity of sperm cells after death.
Materials and Methods: In this experimental study, 24 male Swiss white mice were killed by cervical dislocation and then kept in refrigerator (4-6oC) for up to 12 days. On the 0 (immediately after death as control group), 1st, 2nd, 3rd, 5th, 7th, 10th and the 12th days after death cauda epididymides were removed and squeezed in Ham’s F10 medium. The proportion of viable, motile and double stranded DNA spermatozoa was examined. Viability and DNA integrity of sperm cells were examined consecutively by eosin nigrosin and acridine orange stainings.
Results: The data obtained from this study showed that viability and total motility of sperm cells were significantly decreased during 12 days after death (p<0.001). In contrast with viability and motility, DNA integrity was without significant changes (even 12 days after death).
Conclusion: This study suggests that integrity of sperm DNA would not change even after 12 days after death if the cadaver kept in refrigerator.
Farhad Golshan Iranpour, Soleiman Kheiri,
Volume 14, Issue 2 (2-2016)
Abstract

Background: Lead is an industrial heavy metal that can decrease sperm motility.
Objective: The aim was to investigate the protective effects of calcium against lead on motility of spermatozoa.
Materials and Methods: In total 40 adult male Swiss white mice were randomly divided into 5 groups (control, lead of 1st wk, lead of 2nd wk, lead/calcium of 1st wk and lead/calcium of 2nd wk). The lead groups of mice were injected by a single dose of lead acetate (200 mg/kg) intraperitoneally. Lead/calcium groups of mice were injected by a single same dose of lead acetate along with three doses of 80 mg/kg calcium chloride. The control group of mice was injected only with same volume of distilled water through the same route. Mice of 1st and 2nd wk groups were sacrificed through cervical dislocation one and two weeks after injections respectively.
Results: Mean of the progressive motile spermatozoa of cauda epididymis in lead/calcium group of the first week was higher than the lead group of the first week and this difference was significant. There was not any significant difference among weight of testes and epididymides of all groups.
Conclusion: It can be concluded that calcium can decrease the effects of lead on sperm motility.
Farhad Golshan Iranpour, Khatereh Fazelian, Gholam Reza Dashti,
Volume 15, Issue 10 (12-2017)
Abstract

Background: Nonoxynol-9 a nonionic surfactant is widely used for its spermicidal effects. Finding new sperm immobilizing agents is necessary because Nonoxynol-9 damages the tissues of female reproductive system.
Objective: The aim of this study was to evaluate the effects of Thymoquinone (TQ) as a potential spermostatic compound on the motility and viability of human spermatozoa.
Materials and Methods: In this experimental study, the effects of 5, 10, 20, 50, 100 μg/ml, 1 and 10 mg/ml of TQ on normozoospermic semen samples were investigated. Sperm motility and viability were compared between untreated and TQ-treated aliquots of each semen sample. To evaluate the effects of TQ on the alteration of mitochondrial membrane potential (MMP), 32 semen samples were examined using 50 μg/ml of TQ. Flow cytometric analysis was performed after staining of spermatozoa with JC-1.
Results: Doses above 20 μg/ml of TQ could eventually immobilize all spermatozoa in culture medium. Adding 50 μg/ml of TQ did not significantly diminish the percentage of viable spermatozoa and flow cytometry results revealed that this amount of TQ could decrease sperm MMP.
Conclusion: TQ could discontinue the movement of sperm cells in medium without reducing the population of live spermatozoa. It is more likely that TQ exerts its spermostatic action by mitigating the MMP of spermatozoa. Therefore, TQ could be considered as a potential new natural spermostatic chemical

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