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Showing 4 results for Ehsani

Hamid Nazarian, Marefat Ghaffari Novin, Mohammad Reza Jalili, Reza Mirfakhraie, Mohammad Hassan Heidari, Seyed Jalil Hosseini, Mohsen Norouzian, Nahid Ehsani,
Volume 12, Issue 5 (6-2014)

Background: The Wnt/β- The Wnt/β-catenin signaling pathway is involved in many developmental processes in both fetal and adult life; its abnormalities can lead to disorders including several types of cancers and malfunction of specific cells and tissues in both animals and humans. Its role in reproductive processes has been proven.
Objective: This study was designed to evaluate the expression of the key regulator of this signaling pathway GSK3-β and its presumed role in azoospermia.
Materials and Methods: WNT3a protein concentration and GSK3-β gene expression levels were measured and compared between two groups of infertile men. The test groups consisted of 10 patients with obstructive and 10 non-obstructive azoospermia. The control group was selected among healthy men after vasectomies that were willing to conceive a child using a testicular biopsy technique. Samples were obtained by testicular biopsy and screened for the most common mutations (84, 86 and 255) in the SRY region before analyzing. GSK3-β gene expression was assessed quantitatively by real time-PCR.
Results: The WNT3a protein concentration had no significant difference between the two test groups and controls. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups (p=0.001).
Conclusion: Down-regulation of GSK-3β may cause to non-obstructive azoospermia. Regulation and modification of GSK-3β gene expression by drugs could be used as a therapeutic solution.
Masoud Mobini, Sakineh Mirzaie, Hossein Khorramdelazad, Nahid Zainodini, Zahra Sabzali, Mina Ghyasi, Mitra Mokhtari, Reza Bahramabadi, Hamid Hakimi, Khodayar Ghorban, Maryam Dadmanesh, Vahid Ehsani, Mohammad Kazemi Arababadi,
Volume 13, Issue 6 (8-2015)

Background: It has been documented that cytokines play important roles in the induction of normal functions of the placenta. It has been hypothesized that abnormal expression of the cytokines may be associated with unsuccessful pregnancy.
Objective: The aim of this study was to compare the serum levels of interleukin-17A (IL-17A) and tumor growth factor (TGF-ß) in pre-term, term neonates, and their corresponding mothers.
Materials and Methods: This study was performed on 100 term and 60 pre-term neonates, and also on their corresponded mothers. Serum levels of IL-17A and TGF-ß were examined by enzyme linked immunosorbent assay (ELISA).
Results: Our results revealed that the serum levels of IL-17A were significantly decreased in pre-term neonates in comparison to full-term neonates. However, the serum levels of IL-17A in the mothers either with pre-term or full-term neonates were not different. Also the serum levels of TGF-ß were not changed in pre-term neonates and their mothers when compared with full-term neonates and their mothers, respectively.
Conclusion: Based on these findings, it can be concluded that IL-17A may play crucial roles in induction of normal pregnancies and also probably participates in normal growth of fetus.
Mojdeh Salehnia, Mehri Fayazi, Shokreya Ehsani,
Volume 15, Issue 4 (6-2017)

Background: Concerning the low population of human endometrial mesenchymal cells within the tissue and their potential application in the clinic and tissue engineering, some researches have been focused on their in vitro expansion.
Objective: The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) as a proliferative factor on the expansion and proliferation of human endometrial stromal cells.
Materials and Methods: In this experimental study, the isolated and cultured human endometrial stromal cells from women at ovulatory phase aged 20-35 years, after fourth passage were divided into control and LIF-treated groups. In the experimental group, the endometrial cells were treated by 10 ng/ml LIF in culture media and the cultured cells without adding LIF considered as control group. Both groups were evaluated and compared for proliferation rate using MTT assay, for CD90 marker by flow cytometric analysis and for the expression of Oct4, Nanog, PCNA and LIFr genes using real-time RT-PCR.
Results: The proliferation rate of control and LIF-treated groups were 1.17±0.17and 1.61±0.06 respectively and there was a significant increase in endometrialstromal cell proliferation following in vitro treatment by LIF compared to controlgroup (p=0.049). The rate of CD90 positive cells was significantly increased in LIFtreatedgroup (98.96±0.37%) compared to control group (94.26±0.08%) (p=0.0498).Also, the expression ratio of all studied genes was significantly increased in the LIFtreatedgroup compared to control group (p=0.0479).
Conclusion: The present study showed that LIF has a great impact on proliferation, survival, and maintenance of pluripotency of human endometrial stromal cells and it could be applicable in cell therapies.
Davoud Kianifard, Ali Ehsani, Parisa Zeinolabedini Daneshgar, Ghasem Akbari, Seyyed Maysam Mousavi Shoar,
Volume 17, Issue 11 (November 2019)

Background: Paclitaxel (PTX), a chemotherapeutic agent, and monosodium glutamate (MSG) have oxidative effects on testicular tissue.
Objective: In this study, the effects of MSG administration on the exacerbation of testicular tissue alterations related to PTX treatment were evaluated.
Materials and Methods: MSG (30 & 60 mg/kg i.p.) was administrated to six groups (n = 8/each) of adult mice before or after PTX treatment: control, PTX-treated, MSG30 + PTX, MSG60 + PTX, PTX + MSG30, and PTX + MSG60. Following the euthanizing, the body weight measurement, pituitary–testicular axis hormonal analysis and serum lipid peroxidation index assessment was prepared, testicular histomorphometry (tubular diameter and germinal epithelium height), immunohistochemistry of p53 was completed. Microscopic indices of spermatogenesis (tubular differentiation, spermiogenesis and repopulation indices) were studied.
Results: Body weight was not changed significantly. The levels of testosterone (p = 0.0001), follicle stimulating hormone (p = 0.019), and luteinizing hormone (p = 0.08) were decreased while the level of lipid peroxidation index was increased (p = 0.208) in the treated groups. The histomorphometry indices (p < 0.0001 and p = 0.001, respectively), germ cells population (p < 0.05) and microscopic indices of spermatogenesis (p = 0.001, p = 0.005, p < 0.0001, respectively) were significantly reduced in all treated groups. The administration of MSG before PTX treatment induces more changes. The most positive reaction to p53 was observed in MSG30 or 60 + PTX groups compared to other groups.
Conclusion: The administration of MSG could intensify testicular tissue alterations related to PTX chemotherapy.

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