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Showing 5 results for Bahmanpour

Soghra Bahmanpour, Mohammad Reza Namavar, Tahereh Talaei, Zohreh Mazaheri, Ahmad Monabati,
Volume 7, Issue 3 (7-2009)

Background: Some evidences showed that the secretion of uterine tube vagina and follicular fluid (FF) affects X and Y-chromosome populations. Sperm selection with X or Y chromosome can added to oocyte for gender desired. The isolation of X Y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. The objective of this study was to find if the FF can influence the ratio of X or Y chromosomes therefore we added human FF to culture media to separate X and Y sperms.
Materials and Methods: Normal semen sample from 36 healthy men were selected. Then the samples were divided into control and experimental groups: control group sperms have been incubated with conventional culture media (Ham,s F-10) and experimental group with conventional culture media + 10% human FF. For sperm isolation swim up technique was used. After 24 hours of incubation slides smear were prepared. Then we used the Fluorescent in Situ Hybridization (FISH) method to evaluate the effect of follicular fluid on the population ratio of X and Y containing sperms.
Results: Although the incubation of sperm in FF and Ham,s F-10 increased Y sperm (59.44 % in control and 61.42% in experimental groups) in comparison with X sperm (40.56% in control and 38.5 in experimental groups) significantly (p<0.05), but the Y (or X) bearing sperm did not significantly change in experimental group in comparison with Y (or X) bearing sperm in control group.
Conclusion: This study showed that using the swim up method for collecting sperms and adding FF to culture media can improve some sperm parameters, but did not has significant effects on population of X and Y sperm.
Tahereh Talaei, Tahereh Esmaeelpour, Fatemeh Aekiyash, Soghra Bahmanpour,
Volume 8, Issue 3 (7-2010)

Background: ‍‍Cryopreservation has some detrimental impacts on sperms surface molecules. Modification of the sperm surface molecules can affect on fertility rate. One of the important surface molecules are glycoconjugates.
Objective: The objective of this study was to evaluate the changes of content of the glycocalyx after standard cryopreservation procedure.
Materials and Methods: Forty five healthy semen samples were frozen in 0.5ml plastic straws and kept in liquid nitrogen and thaw after 48 hours. Sperm smears were prepared before and after freezing and thawing. The smears were stained with the lectins and also with acridin orange. The smears were studied by fluorescents microscopy and the intensities of the reactions to lectins were measured by image analyses software.
Results: The reactions of the sperm samples to Peanut agglutinin (PNA) Wheat germ agglutinin (WGA) and Dolichos biflorus (DBA) changed after cryopreservation and the percentage of samples that showed modifications were 46.67% 34.09% and 73.34% respectively. The crypreservation led to both increase and decrease the intensities of the reactions. It means that there are various mechanisms that impact on the carbohydrate contents of the sperm surface. There is no correlation between DNA denaturation of sperms and their lectin binding patterns.
Conclusion: Cryopreservation affected the surface glycoconjugates at least in a subset of spermatozoa. These results might cause to modify the future application of sperm banking techniques.
Tahereh Talaei-Khozani, Zahra Borzoei, Soghra Bahmanpour, Jaleh Zolghadr, Sedigheh Dehbashi, Hamid Reza Zareh,
Volume 9, Issue 2 (7-2011)

Background: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities.
Objective: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.
Materials and Methods: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test.
Results: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant.
Conclusion: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility
Tahereh Esmaeilpour, Leila Elyasi, Soghra Bahmanpour, Alireza Ghannadi, Ahmad Monabbati, Farzaneh Dehghani, Marjaneh Kazerooni,
Volume 10, Issue 5 (10-2012)

Background: It has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. These methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques.
Objective: This study compared simple density gradient methods and a combined method with albumin density gradient and PureSperm separation (alb/PureSperm) for sex preselection by double fluorescence in situ hybridization (FISH) versus chromomycin A3 staining to determine chromatin integrity. Materials and Methods: 30 normal semen samples were prepared with PureSperm, albumin gradients and alb/PureSperm. All samples were then stained by FISH and chromomycin A3. The results were compared with SPSS 11.5 and the Kruskal-Wallis test.
Results: The proportion of X-bearing spermatozoa by PureSperm separation (47.58±5.67) and Y-bearing spermatozoa by albumin gradient (46.13±3.83) methods were slightly higher than in putative normal sperm samples (1:1), but there were no significant differences in the X- or Y- bearing spermatozoa counts among the three methods. Albumin gradient separation tended to underestimate abnormal spermatozoa compared to PureSperm and combined alb/PureSperm.
Conclusion: Routine separation methods slightly enriched X- or Y- bearing spermatozoa, but the differences were not significant for clinical purposes. The combined alb/PureSperm method had no advantages for assessing sex ratio or chromatin integrity compared to simpler gradient methods.
Soghra Bahmanpour, Tahereh Talaei Khozani, Nehleh Zarei Fard, Mansoureh Jaberipour, Ahmah Hosseini, Tahereh Esmaeilpour,
Volume 11, Issue 10 (12-2013)

Background: The interaction between follicular cells and oocyte leads to a change in gene expression involved in oocyte maturation processes.
Objective: The purpose of this study was to quantify the expression of more common genes involved in follicular growth and oocyte developmental competence.
Materials and Methods: In this experimental study, the expression of genes was evaluated with qRT-PCR assay in female BALB/c mice pups at 3-day of pre-pubertal and 8 week old virgin adult ovaries. The tissue was prepared by H&E staining for normal morphological appearance. The data were calculated with the 2-ΔCt formula and assessed using non-parametric two-tailed Mann-Whitney test. The p<0.05 was considered as significant.
Results: The data showed a significant increase in the level of Stra8 and GDF9 in adult compared with newborn mice ovaries (p=0.049). In contrast, a significant decrease in the level of Mvh, REC8, SCP1, SCP3, and ZP2 was observed in adult mice ovaries compared to those in the newborn mice ovaries (all p=0.049 except SCP1: p=0.046). There was no significant difference in the level of OCT4 and Cx37 expression between adult and newborn mice ovaries.
Conclusion: The modifications in gene expression patterns coordinate the follicular developmental processes. Furthermore, the findings showed higher expression level of premeiotic gene (Stra8) and lower level of meiotic entry markers (SCP1, SCP3, and REC8) in juvenile than newborn mouse ovaries.

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