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Showing 67 results for Amid

Ashraf Moini, Kiarash Riazi, Vida Amid,
Volume 2, Issue 1 (7-2004)
Abstract

Pelvic inflammatory disease (PID) is a rare complication of transvaginal oocyte retrieval. It may result in failure of assisted reproductive techniques (ART). During a 7 years period, 5958 transvaginal ultrasound-guided oocyte retrievals resulted in 10 cases of acute PID. Eight out of 10 patients were diagnosed infertile because of endometriosis. Two patients had mild ovarian, 3 had stage III, and 2 had stage IV endometriosis. One patient had a 3-4 cm ovarian endometrioma. After treatment, no mortality was encountered among the 10 patients, although none of them conceived. This observation supports the previous reports that endometriosis can raise the risk of PID after oocyte retrieval. More vigorous antibiotic prophylaxis and better vaginal preparation are recommended when oocyte pickup is performed in patients with endometriosis.
Mitra Bakhtiari, Aligholi Sobhani, Mohammad Akbari, Parichehr Pasbakhsh, Mehdi Abbasi, Azim Hedayatpoor, Fardin Amidi , Feridoon Sargolzaei,
Volume 5, Issue 3 (7-2007)
Abstract

Background: Various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes.
Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing.
Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 µg/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate.
Results: HA supplementation (750 µg/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 µg/ml HA was also positive on the sperms. But 1,250 µg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.
Conclusion: The dose of 750 µg/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.

 
Morteza Koruji, Mansoureh Movahedin, Seyed Javad Mowla, HAmid Gourabi,
Volume 5, Issue 4 (7-2007)
Abstract

Background: The basis of spermatogenesis is the spermatogonial stem cells (SSCs). The concentration of SSCs is very small. However, a system that supports the proliferation and maintenance of SSCs in vitro could be used to preserve and expand SSCs numbers as well as increase success in transplantation. It is a new avenue to restore spermatogenesis in azoospermia subjects.
Objective: Proliferation and enhancement of frozen-thawed SSCs numbers during in vitro culture.
Materials and Methods: Both Sertoli and spermatogonial cells were isolated from adult mouse testes. Frozen-thawed spermatogonial cells were cultured in two groups: simple culture (Experimental 1) and co culture with Sertoli cells (Experimental 2). Also, Fresh cells were considered as control groups: simple culture (control1) and co culture with Sertoli cells (control 2).Assay of the spermatogonial-cell-derived colonies was carried out at the end of each week.
Results: Results indicated that the viability rate of the frozen cells after thawing (68.4±10.2%) was influenced by cryopreservation procedure significantly (p ≤0.001). In addition, the number of the colonies and their diameters in the co-culture system with fresh cells (25.1±5.2 and 205.8±50 µm, respectively) were more than other groups and the differences were significant (p<0.001). Number of the colonies and their diameters in experimental 1(9.5±4.3 and 124±35.9 µm, respectively), experimental 2 (15.6±3.5 and 157.6±41.9µm, respectively) groups were better than control 1 group (3.1±2.2 and 87.5±30.6µm, respectively) and the differences were significant (p<0.001).
Conclusion: We demonstrated that co-culture system with Sertoli cells can increase in vitro colony formation of adult fresh and frozen-thawed spermatogonial cells in mouse.
 
 
Bibi Shahnaz Aali, HAmid Abdollahi, Nouzar Nakhaee, Zohreh Davazdahemami, Anahita Mehdizadeh,
Volume 5, Issue 5 (7-2007)
Abstract

Background: Group B streptococcus is regarded as a potential factor for adverse outcomes of pregnancy such as preterm birth.
Objective: To study the association of maternal vaginal colonization with group B streptococcus (GBS) and preterm labor.
Materials and Methods: From April 2005 to May 2006, vaginal culture for GBS were conducted in 101 laboring women with a gestational age of 24-37 weeks and 105 women admitted for term delivery at maternity center of Afzalipour Hospital in Kerman, Iran. Student`s t test and Chi square test were used to compare continuous and categorical data between the groups. Using multivariate logistic regression the association between GBS colonization and preterm labor was analyzed. P-values<0.05 were considered as significant.
Results: Colonization was detected in 9.2% of all mothers. Although GBS colonization was found more frequently in preterm than term patients (12 v/s 7 cases), the difference was not statistically significant. However, GBS positivity was roughly associated with preterm labor. Age was also a risk factor for GBS colonization. No case of perinatal sepsis occurred during the study period.
Conclusion: Maternal colonization for GBS is relatively low in our center. Increasing age enhances the risk of colonization. Vaginal colonization of GBS is relatively associated with preterm labor.
Musa Toyin Yakubu, Musbau Adewumi Akanji, Adenike Temidayo Oladiji, Abdulwaheed Olajide Olatinwo, Abdulfatai Ayoade Adesokan, Monsurat Oyenike Yakubu, BAmidele Victor Owoyele, Taofik Olatunde Sunmonu, Moyosore Salihu Ajao,
Volume 6, Issue 4 (7-2008)
Abstract

Background: The increase in the rate of infertility in women has prompted the need to search for plants with antifertility potentials.
Objective: The effect of administration of aqueous extract of Cnidoscolous aconitifolius leaves for seven days on reproductive hormones of female Wistar rats was investigated.
Materials and Methods: Sixty female albino rats were grouped into four (A, B, C and D) of 15 each. Group A (the control) received orally 6.5 ml kg-1 body weight of distilled water on daily basis for 7 days. Groups B, C and D were treated like the control except that they received 250, 500 and 1000 mg kg-1 body weight of the extract.
Results: Phytochemical screening of the extract revealed the presence of alkaloids, saponins, phenolics, tannins, flavonoids, anthraquinones, phlobatannins and triterpenes. Administration of the extract produced significant increase (p<0.05) in the serum prolactin concentration whereas those of estradiol, progesterone, follicle stimulating and luteinizing hormones were significantly reduced.
Conclusion: The alterations in the female rat reproductive hormones by the extract are indications of adverse effect on the maturation and ovulation of follicles. Consequently, the extract may impair fertility and conception in female rats. Thus, the Cnidoscolous aconitifolius leaf extract may be explored as a female contraceptive.
HAmid Reza Momeni, Malek Soleimani Mehranjani, Mohammad Hosien Abnosi, Monireh Mahmoodi,
Volume 7, Issue 3 (7-2009)
Abstract

Background: para-nonylphenol (p-NP) is able to induce malformations in male reproductive system.
Objective: The aim of this study was to investigate the preventing role of vitamin E (Vit.E) on sperm parameters and reproductive hormones in developing rats.
Materials and Methods: Pregnant rats were divided into 4 groups: control p-NP Vit.E and p-NP+Vit.E. Treatments were performed on day 7 of gestation and continued during weaning. The male pups were then divided into the same groups as the mothers and were treated till 90 days of age. Finally body and left testis weight were recorded and left epididymis was cut in Ham’s F10. Released sperm were used to analyze number motility and viability of the sperm. Blood serum was used to assess follicle stimulating hormone (FSH) luteinizing hormone (LH) estrogen and testosterone.
Results: In p-NP-treated rats a significant decrease was found in body and testis weight sperm number and sperm motility compared to control and p-NP+Vit.E groups. A significant increase was also found in sperm viability in Vit.E group (83.3±7.6) compared to both p-NP (59.5±7.5) and control (66.3±9.7) groups. Rats treated with p-NP showed a significant decrease in FSH level and a significant increase in estrogen level. However testosterone and LH level remained constant. In p-NP+Vit.E group the change of estrogen level but not FSH was significantly reversed compared to p-NP group. Conclusion: Vit.E not only is able to compensate the toxic effects of p-NP on testis weight sperm number sperm motility and estrogen level but also increases sperm viability in developing rat.
Mohammad Ali Khalili, Najmeh Zare-Zadeh, HAmideh Hashemi,
Volume 7, Issue 3 (7-2009)
Abstract

Background: One of the main laboratory tests for evaluation of infertility is semen analysis (SA). However additional tests may be suggested for further diagnosis of male fertility potentials. The seminal fluid contains sperms non-sperm cells and various types of lipids and glucose.
Objective: The objective of this cross-sectional study was to correlate the sperm parameters with concentrations of cholesterol triglyceride LDL and HDL in serum samples of infertile men with abnormal SA.
Materials and Methods: A total of 120 infertile men (aged 23-49 years) with abnormal SA were enrolled for this cross-sectional study. Sperm concentration and motility was evaluated using Makler chamber. While normal morphology was done after Geimsa staining. Following 12 h of fasting the blood samples were obtained for evaluation of cholesterol triglyceride LDL and HDL levels. The lipid profiles were compared with the rates of normal and abnormal sperm parameters. Chi-square and fisher exact tests were used for data evaluation.
Results: 75.5% and 98% of the subjects with normal levels of triglyceride had abnormal sperm morphology and progressive motility respectively. Also abnormal levels of triglyceride and cholesterol were related with abnormal sperm morphology and motility. The levels of LDL and HDL were normal in 80% and 89.3% of the cases respectively. The majority of the patients with normal LDL had abnormal sperm parameters.
Conclusion: The results showed that the concentrations of serum lipids were not generally related with the quality of semen parameters. Further studies on the role of lipid profiles of infertile men with sperm fertilizing potentials are necessary.
Malek Soleimani Mehranjani, Ali Noorafshan, Ahmad Hamta, HAmid Reza Momeni, Mohammad Hussein Abnosi, Monireh Mahmoodi, Morteza Anvari, Maryam Hazaveh,
Volume 8, Issue 1 (7-2010)
Abstract

Background: Para-Nonylphenol (p-NP) is one of the environmental pollutants which cause reproductive system disorders.
Objective: The effects of vitamin E on ovary structure during its development in rats treated with p-NP.
Materials and Methods: 32 Wistar female rats after mating were divided into 4 groups; control vitamin E (100mg/kg/day) p-NP (250mg/kg/day) and p-NP + vitamin E. The rats were treated from the day 7 of pregnancy till 21st day of postnatal through sucking period. After weaning the female pups were treated by gavages for 120 days. The total volume of ovary number of follicles volume of oocyte follicular cells and their nuclei and the thickness of zona pellucida were estimated stereologically. The results were analyzed using one way ANOVA and p<0.05 was considered significant.
Results: The ovary weight, mean total volume of ovary and cortex, number of antral and graafian follicles and body weight were decreased significantly (p<0.05) in the p-NP treated rats compared to control and other groups, while the number of atretic follicles was increased significantly (p<0.05). A significant reduction (p<0.05) in volume of oocyte, follicular cells and their nuclei in antral and graafian follicles was found in p-NP group. In addition, treatment with only vitamin E showed an improving effect on folliculogenesis due to a highly significant increase (p<0.01) in the number of primordial follicles.
Conclusion: Vitamin E could compensate the adverse effects of p-NP on the ovary structure during its development.
Habibollah Johari, Mehrdad Shariati, Shahram Abbasi, Esfandyar Sharifi, HAmid Reza Askari,
Volume 8, Issue 3 (7-2010)
Abstract

Background: Diazinon is a widely used Organophosphate insecticide which is applied against plant pests. This compound has various side effects because it acts as an acetyl cholinesterase enzyme inhibitor.
Objective: The aim of present study was to investigate the effects of diazinon on pituitary–gonad axis and ovarian histological changes in rats.
Materials and Methods: In total 50 female wistar rats were divided into 5 groups of 10 including control sham and experimental groups I II and III which orally received 50 100 and 150 mg/kg/bw diazinon for 14 days respectively. Diazinon was administered orally and 24 hours after the last treatment blood samples were taken from the heart centrifuged and sera were evaluated for the concentrations of estrogen progesterone and gonadotropins via RIA method. In addition ovaries were removed fixed and studied with steriological methods.
Results: The results show no significant changes in body weight among various groups; while ovarian weight in experimental group III decreased significantly (p<0.05). Also, no significant changes in the levels of LH, FSH and estradiol hormones were observed. In contrast, the concentration of progesterone showed a significant decrease in all experimental groups compared with the control group. The results showed no significance difference in the mean number of primary, secondary and graffian  follicles but there was a significant decline in the mean number of corpus luteum in experimental group receiving 150 mg/kg diazinon (p<0.05 ) .
Conclusion: One can conclude that oral administration of diazinon could have adverse effect on progesterone hormone level as well as hazardous effects on ovarian tissues and reproductive processes.
Mohammad Reza Nowroozi, Keivan Radkhah, Alireza Ranjbaran, Saeed Reza Ghaffari, Mohammad Ali Sedighi Gilani, HAmid Gourabi,
Volume 8, Issue 4 (7-2010)
Abstract

Background: The sperm count and function may be affected by karyotype abnormalities or microdeletion in Y chromosome. These genetic abnormalities can probably transmit to the children.
Objective: In this study, we tried to determine the frequency of karyotype abnormalities and Y chromosome microdeletions in severe oligospermic or azoospermic men who fathered sons by ICSI.
Materials and Methods: This study comprised of fathers who had at least a son with ICSI due to severe oligospermia or azoospermia. General examinations were done and blood sample were obtained for karyotype and Y chromosome studies.
Results: The total of 60 fathers was evaluated along with their 70 sons. The mean duration of infertility was 8.7 years and the sons were 2.4 years in average at the time of examination. The mean age of neonates at the time of delivery was 33 weeks; 42.9% were delivered prematurely; and 40.5% of them were twins. 8.6% of the sons had hypospadiasis and 7.1% had UDT. Most of the side effects were due to prematurity. In total 6 of fathers had karyotype anomaly, meanwhile 4 of their sons had also karyotype anomaly. Only one son had karyotype anomaly without affected father. No case of Y chromosome microdeletion was found in the fathers.
Conclusion: Y chromosome microdeletion is not prevalent in fathers with successful ICSI and it is not necessary to be analyzed before ICSI performance. Karyotype anomaly may transmit to the sons. All together ICSI is reliable and safe. Most of the complications are the result of premature delivery.
Tahereh Talaei-Khozani, Zahra Borzoei, Soghra Bahmanpour, Jaleh Zolghadr, Sedigheh Dehbashi, HAmid Reza Zareh,
Volume 9, Issue 2 (7-2011)
Abstract

Background: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities.
Objective: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility.
Materials and Methods: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test.
Results: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant.
Conclusion: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility
Mohammad Baqer Minaei, Mohammad Barbarestani, Saeid Nekoonam, Mir Abbas Abdolvahabi, Nasrin Takzare, Mohammad Hossein Asadi, Azim Hedayatpour, Fardin Amidi,
Volume 10, Issue 2 (7-2012)
Abstract

Background: Sperm parameters and motion kinetics are affected by cryopreservation.
Objective: The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter.
Materials and Methods: Semen was collected from 20 normal donors and divided into five aliquots prior to cryopreservation. The first aliquot was analyzed by computer-assisted sperm analysis (CASA). Other aliquots were mixed with cryo-protective agent containing 0, 20, 40, and 80 µmol Trolox and treated samples were cryopreserved in liquid nitrogen. After two weeks samples were thawed and sperm motion kinematics was measured by CASA. Percent motility (Mot), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), and amplitude of lateral head displacement (ALH) were compared before and after freeze.
Results: Addition of 40µmol Trolox resulted in significantly higher (p<0.05) post thaw VCL, VSL and VAP compared to other groups. Therefore the percentage of post thaw motile spermatozoa were significantly higher (p<0.01).
Conclusion: The supplementation of Trolox significantly improved the post-thawed human semen quality, especially progressive motility and average path velocity.
HAmid Reza Momeni, Najmeh Eskandari,
Volume 10, Issue 3 (7-2012)
Abstract

Background: Arsenic as an environmental toxicant is able to exert malformations in male reproductive system by inducing oxidative stress. Vitamin E (Vit.E) is known as antioxidant vitamin.
Objective: The aim of this study was to investigate the harmful effects of sodium arsenite on sperm parameters and the antioxidant effects of Vit.E on sperm anomalies in sodium arsenite treated rats.
Materials and Methods: Adult male rats were divided into 4 groups: control, sodium arsenite (8 mg/kg/day), Vit.E (100 mg/kg/day) and sodium arsenite+Vit.E. Oral treatments were performed till 8 weeks. Body and left testis weight were recorded and then left caudal epididymis was cut in Ham's F10. Released spermatozoa were used to analyze number, motility, viability and abnormalities of the sperm. Sperm chromatin quality was assessed by nuclear staining using acridine orange and aniline blue.
Results: Body and testis weight showed no significant change in 4 groups (p>0.05). A significant decrease in the number, motility, viability and normal sperm morphology was found in sodium arsenite-treated rats compared to the control (p<0.001). Sodium arsenite had no effect on sperm DNA integrity and histon-protamine replacement (p>0.05). In sodium arsenite+Vit.E group, Vit.E could significantly compensate the harmful effects of sodium arsenite on sperm number, motility, viability and morphology compared to sodium arsenite group. In addition, sperm viability and motility was significantly increased in rats treated with Vit.E alone compared to the control and sodium arsenite+Vit.E group.
Conclusion: Vitamin E could compensate the adverse effects of sodium arsenite on sperm parameters in adult rats.

Elham Siasi, Ahmad Aleyasin, Javad Mowla, HAmid Sahebkashaf,
Volume 10, Issue 4 (8-2012)
Abstract

Background: Histones are replaced by protamines to condensate and package DNA into the sperm head during mammalian spermatogenesis. Protamine genes defects have been reported to cause sperm DNA damage and male infertility.
Objective: In this study relationship among some protamines genes family SNPs include PRM1 (C321A), PRM2 (C248T) and TNP2 (T1019C), (G1272C), (G del in 1036 and 1046 bp) were studied in 96 idiopathic infertile men with azoospermia or oligospermia and 100 normal control men.
Materials and Methods: Analysis of SNPs was performed using restriction fragment length polymorphism (PCR-RFLP), single strand conformational polymorphism (PCR-SSCP) and PCR sequencing.
Results: No polymorphisms were found for tested SNPs except for PRM1 (C321A) and TNP2 (G1272C) in which frequency of altered AA and GG genotypes were slightly higher in infertile case group. Statistical analysis showed no significant association related to PRM1 (C321A) p=0.805 and TNP2 (G1272C) loci p=0.654.
Conclusion: These results are consistent with previous studies and indicating that all tested SNPs was not associated with oligospermia and azospermia and idiopatic male infertility in Iranian population.
Reza Mahmoudi, Farzad Rajaei, Iraj Ragardi Kashani, Mehdi Abbasi, Fardin Amidi, Aligholi Sobhani, Iraj Amiri,
Volume 10, Issue 5 (10-2012)
Abstract

Background: Cryopreservation and in vitro maturation (IVM) of oocyte is becoming an important technique in infertility treatment and fertility preservation. Also it has been proposed to establish a genetic resource bank for endangered or commercially important animal species.
Objective: The aim of this study was to evaluate viability, maturation and fertilization rate of mouse immature oocytes after single and stepwise vitrification procedure.
Materials and Methods: Oocytes were obtained from 4 weeks old female mice 48h after intraperitoneal injection of 7.5 IU pregnant mare serum gonadotropin (PMSG). Collected oocytes before vitrification were exposed to cryoprotectant, which was composed of 30% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose, either by single step or in a step-wise way. After vitrification and storage in liquid nitrogen, the oocytes were warmed and washed two times in medium TCM199 and then subjected to IVM, fertilization and subsequent development to blastocysts.
Results: The oocytes survival rates after vitrifying-warming (88.96%), maturation rate (73.23%), the capacity of fertilization (57.80%) and embryonic development to blastocyst (16.41%) in the step-wise exposure were significantly higher (p<0.001) compared with corresponding rate in the single step procedure.
Conclusion: The results suggest that vitrification with step-wise procedure has positive effects on maturation and developmental capacity of mice germinal vesicle oocytes in compare with single step vitrification procedure.
Ali Cyrus, Hassan Solhi, Mahdi Azizabadi Farahani, HAmid Reza Khoddami Vishteh, Davoud Goudarzi, Ghasem Mosayebi, Hamed Mohamadian,
Volume 10, Issue 6 (4-2012)
Abstract

Background: Given the paucity of data on possible testis changes in opioid dependents, we sought to compare the testis volumes between a group of opium dependents and a group of healthy controls.
Objective: Comparison of testis volume between opium dependents and healthy controls.
Materials and Methods: This case-control study recruited 100 men with opium dependency (cases) and 100 healthy men (controls) in Iran, in 2008. A checklist containing questions about age, height, weight, daily amount of cigarette use, and duration of cigarette use for all the participants as well as daily amount of opium use (grams) and duration of opium use (years) for the case group was completed. Additionally, the dimensions of each testis were measured by a single person using calipers, and the mean of the left and right testes volume was compared between these two groups.
Results: The mean of the testis volumes in the case group was significantly lower than that of the case group (11.2±2.2 and 25.1±2.7cm³, p<0.001). The results of the ANCOVA test showed that even after the omission of the cigarette smoking effect (p=0.454), the testis volume remained lower in the opium dependents (R2=0.884, p<0.001). In the case group, there were significant reverse correlations between testis volume and age (r=-0.404, p<0.001), daily amount of opium use (r=-0/207, p=0.039) and duration of opium use (r=-0.421, p<0.001).
Conclusion: We found that the testis volume in the male opium dependents was lower than that of the healthy controls. We would recommend that future studies into the impact of drugs on the testis dimensions pay heed to possible histological changes in the testes owing to opium dependency.
Nourossadat Kariman, Maryam Afrakhte, Mehdi Hedayati, Masoumeh Fallahian, HAmid Alavi Majd,
Volume 11, Issue 2 (4-2013)
Abstract

Background: Rupture of fetal membranes can occur at any gestational age. Premature rupture of membranes (PROM) means rupture of fetal membranes before the onset of labor.
Objective: The purpose of this study was to evaluate and compare the reliability of the vaginal washing fluid urea and creatinine for the diagnosis of PROM and to determine cut-off values.
Materials and Methods: A total of 179 pregnant women were recruited. All patients underwent different examinations. These included nitrazine paper test, fern test, amniotic fluid pooling, vaginal washing fluid urea and creatinine sampling. The one group consisted of 126 pregnant women between 14 and 41 weeks of gestation with the complaint of vaginal fluid leakage. Patients who had positive pooling, nitrazine paper test and fern test were considered as confirmed PROM group (group 1). On the other side, patients with pooling (-) and/or nitrazine paper test (-) and/or fern test (-) were taken as suspected unconfirmed PROM cases (group 2). The control group consisted of 53 pregnant women between 14 and 41 weeks of gestation without any complaint or complication. Weconducted one-way ANOVA test on the urea and creatinine measures and post-hoc comparison test. Cut-off value was determined by receiver operating characteristic (ROC) curve.
Results: Vaginal fluid concentrations of urea and creatinine were significantly different between the three groups (p<0.001). The sensitivity, specificity, positive and negative predictive values and accuracy were all 100% in detecting premature rupture of membranes by evaluation of vaginal fluid creatinine concentration with a cut-off value of 0.45 mg/dl, respectively.
Conclusion: This study demonstrates that of two markers investigated creatinine has the higher diagnostic power.
Mohammad Hasan Sheikhha, Mohammad Ali Zaimy, Saeede Soleimanian, Seyed Mehdi Kalantar, Azam Rasti, Maryam Golzade, HAmid Hoseini Fahraji,
Volume 11, Issue 4 (6-2013)
Abstract

Background: It has been hypothesized that Y-q microdeletion can account for significant proportion of infertility in men. There are three nonoverlapping regions referred to as the "azoozpermia factors" AZFa, AZFb, and AZFc from proximal to distal part of Y-q. These have been defined as spermatogenesis loci, this region deletions have been shown to be involved in male azoospermic or severe oligoozospermic infertility.
Objective: Evaluation the rate of Y-chromosome microdeletions in infertile men.
Materials and Methods: In this case-control study, 25 azoospermic infertile men candidate for intracytoplasmic sperm injection (ICSI) were selected as case group. For control group, 25 normoozoospemric men were selected. All cases and controls had normal 46XY karyotype. DNA extraction and molecular analysis were done on blood samples. Multiplex-PCR method was done to identify the presence of microdeletion in AZFa, AZFb or AZFc loci. Eight STS primers that include two controls were selected to determine Y-chromosome microdeletions.
Results: 20% (5/25) of all patients have at least one microdeletion in more than one region of AZF loci. Totally 17 microdeletions was observed, one case had deletions in three AZF regions, and 4 cases had deletions in two AZF regions. The rate of deletions was 42% (7/17) for AZFc, 35% (6/17) for AZFa and 23% (4/17) for AZFb.
Conclusion: The molecular DNA analysis could help us to know the real cause of infertility and can give good information for good decision for example in men whit microdeletions who want to undertake ICSI procedure the deletions will be passed to their son.
Fatemeh Pourkeramati, Haleh Soltanghoraee, Naser Amirjannati, Mohammad Mehdi Akhondi, HAmid Reza Khorram Khorshid,
Volume 11, Issue 4 (6-2013)
Abstract

Background: Cryptorchidism is a common malformation in neonates; surgery or medical treatments are applied during childhood. Untreated cryptorchid testes are in the risk of intratubular germ cell neoplasia (IGCN) and consequently invasive testicular tumors which could be shown by immunohistochemistry staining for placental like acid phosphatase (PLAP) marker.
Objective: We designed this study to know the prevalence of IGCN in untreated cryptorchid testes of infertile men, in our infertility center as a refferal center.
Materials and Methods: In this cross-sectional study we assessed H&E slides of testicular samples of 13 adult infertile patients with impalpable intra-abdominal testes seeking infertility treatment; then we stained them by PLAP marker.
Results: Three (23.08%) samples were positive for PLAP marker means presence of IGCN in testis. One of them showed seminoma besides IGCN. Conclusion: According to the results of this study and the fact that there are adult untreated cryptorchid patients in our country yet, it is suggested to pay more attention in clinical examination, assessment and follow up of such patients for malignancy screening.
Shahla Sohrabipour, Adele Jafari, Mohamad Kamalinejad, Abdolfatah Sarrafnejd, Taherah Shahrestany, HAmid-Reza Sadeghipour,
Volume 11, Issue 6 (9-2013)
Abstract

Background: Prepubertal varicocele has the most devastating effects on the testes. Oxidative stress is the major cause leading to infertility in varicocele. The antioxidant properties of Flaxseed (FS) treatment in some oxidative diseases have been reported.
Objective: This study aimed to evaluate the antioxidant effect of FS in prepubertal rats with experimental varicocele.
Materials and Methods: Forty two male prepubertal rats were divided into 6 groups: the varicocele group were either fed with 10% FS, or with regular diet, or with Vit E, the group with sham operation fed with 10% FS, or had regular diet, and control rats who had not been operated but received regular diet. Varicocele was created by Koksal method. After 6 weeks sperm superoxide anion and H2O2 were evaluated by flowcytometery. Semen total antioxidant capacity (TAC) by Koracevic method and testes malondialdehyde (MDA) by thiobarbituric acid with spectrophotometry was measured.
Results: While superoxide anion and H2O2 were significantly higher in varicocele grop with regular diet (p=0.0001), FS significantly decreased the previously-mentioned parameters (p=0.0001). There were no significant differences for seminal TAC between 6 groups (p=0.07). Left testicular MDA concentration were lower in varicocele or group that were fed with 10% FS compared with other groups (p=0.001).
Conclusion: Reactive oxygen species (ROS) may cause sperm oxidative damage. FS as a fat soluble antioxidant can scavenge intracellular ROS production in varicocele.

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