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Showing 10 results for Bahar

Houshang Babolhavaeji, Seyed Habibollah Mousavi Bahar, Nahid Anvari, Farhang Abed, Arash Roshanpour,
Volume 6, Issue 3 (7-2008)

Background: Many azoospermic patients with non obstructive azoospermia (NOA) are candidate for testicular sperm extraction (TESE) and in vitro fertilization. Because sperm might be present in some but not all parts of the testes of such men, multiple sampling of testicular tissue are usually necessary to increase the probability of sperm finding. Sperm finding can be done by two methods: 1) classic histopathology and 2) wet smear.
Objective: Comparative study of pathology and wet smear methods for discovering sperm in testis biopsy of azoospermic men.
Materials and Methods: We prospectively studied 67 consecutive infertile men who referred to Fatemieh Hospital, Hamedan, Iran between April 2002 and September 2004. All patients were either azoospermic or severely oligozoospermic. They underwent intraoperative wet prep cytological examinations of testis biopsy material and then specimens were permanently fixed for pathologic examination too.
Results: Among the 67 testes that underwent wet prep cytological examination, 44 (65.7%) were positive and 23 (34.3%) had no sperm in their wet smear. On the permanent pathologic sections, 19 (28.4%) were positive and 48 (71.6%) cases were with no sperm in their sections. Among all the individuals 18 (26.8%) were negative in both studies, while 14 (20.8%) had minimum 1 sperm in their smears in both examinations. The positive cases in wet prep cytological examination were significantly more than the cases in the permanent histopathologic sections (p-value=0.000).
Conclusion: It seems that wet prep cytological examination is more reliable than permanent histopathologic sections in detecting sperm in testis biopsy of azoospermic men.
Seyed Gholam Ali Jorsaraei, Hiroaki ShiBahara, Ayustawati , Yuki Hirano, Yasuko Shiraishi, Ali Khalatbari, Yousofreza Yousofnia Pasha, Mitsuaki Suzuki,
Volume 6, Issue 4 (7-2008)

Backgrand: Cotinine (COT) is a major degradation product of nicotine (NIC). The participation of leptin in female reproduction is well-established, but any role in male reproductive function is at the best tenuous.
Objective: The aim of this study was to evaluate the in-vitro effects of nicotine, cotinine and leptin on sperm parameters in normal semen of non-smokers fertile men.
Materials and Methods: Ten healthy nonsmokers aged 25-40 years old were devided into 7 groups, Thier semens were divided into 7 aliquots. (A) was layered with basal solution 70 ng/ml NIC, (B) 35 µg/ml NIC, (C) 300 ng/ml COT, (D) 200 µg/ml COT, (E) 30 ng/ml leptin, (F) 300 ng/ml leptin respectively and (G) was layered with mHTF. After migration, the samples were examined at time 0, +1, +2, +4, +8, and + 24 h of incubation.
Results: These findings were obtained: sperm count: 75.66±66.25x10 6 /ml, forward motility: 75.55±14.80%, progress: 33.66±13.01, VSL: 51.58±6.99 µm/s, VCL: 103.33±14.52 µm/s, ALH: 4.33±0.77 µm, BCF: 25.60±2.97 HZ, STR: 79.33±8.04 %, LIN: 52.55±10.52 %, ELO: 74.22 ± 12.76 % and ARE: 3.04 ± 1.50 u/sq. The parameters were similar before 8 hr and were being decreased after that.
Conclusion: According to the results. nicotin and cotinin have negative effects on the sperm parameters but despite the positive effect of leptin, there is no correlation between leptin concentration in semen and its physical characteristics.
Mona Zvâncă, Radu Vlădăreanu, Asim Kurjak, Awatif Juma Al Bahar,
Volume 6, Issue 4 (7-2008)

Background: With the technical development, ultrasound tends to overpass its limits and to become the main investigation tool even for situations when it was traditionally considered second best. It is more convenient both for patient and doctor, less expensive and has real diagnostic value.
Objective: The present paper, and its graphic back-up, are intended as a visual testimony of the fact that the newer three dimensional (3D) ultrasound technique is much more than a simple generator of nice, commercial, images. Moreover, its use may be extended from obstetrics to gynecological pathology, especially in cases related to infertility.
Materials and Methods: We performed a review of the main local infertility causes and their perception by the 3D ultrasound, as compared to the conventional methods. General literature citations were completed with personal cases and images. In our research, we used a Kretz Voluson Expert 730 ultrasound machine with a full logistic equipment. Our database was completed over a period of 18 months and included various pathology, such as uterine anomalies, fibroids, polyps, ovarian cysts and many others.
Results: Three dimensional ultrasound represents the best tool in evaluating the uterine cavity, the endometrium, assessing its volume and vascularity pattern. It also offers a very good image of the uterine structure, the adnexal morphology and their relationship. It performs a thorough pelvic assessment by a single examination. Moreover, it may realize a histerosalpingography, which is as efficient as the radiological method, but easier and with fewer side effects.
Conclusion: Even though it is technically more difficult and time consuming, a good practice and high quality ultrasound equipment offer a series of benefits over any other kind of investigation.
Mohamad Reza Darabi, Mohamad Hosein Nasr-Esfahani, Hosein Baharvand, Mohmad Mardani, Hojatolah Karimi-Jashni,
Volume 6, Issue 5 (7-2008)

Background: The values of embryonic stem cell and cloning are evident. Production of clone from embryonic stem cells can be achieved by introduction of stem cell into a tetraploid blastocyst. Tetraploid blastocyst can be produced in vitro by electrofusion of 2-cell embryos.
Objective: The aim of this study was to assess the effect of different voltages and durations on fusion rate of bovine 2-cell embryos and their subsequent development in vitro.   
Material and Methods: The in vitro produced bovine 2-cell embryos were categorized into 3 groups: (1) fused group (FG); 2-cell embryos fused by exposure to different voltages (0.5, 0.75, 1, 1.25 and 1.5 kV/cm) and durations (20, 40, 60, 80 and 100 μs), (2) exposed control group (ECG);  2-cell embryos exposed to different voltages and durations but remained unfused and (3) unexposed control group (UCG); embryos cultured without exposure to any voltage. The embryos from each group were cultured and fusion, cleavage and developmental rates were compared in each group.
Results: The results show that increased voltage, increases the fusion rate up to 88% for 1.5 kV/cm; however, the rate of cleavage and blastocyst formation decreases significantly to 18% and 10% respectively (p<0.05). Increased duration does not significantly increase fusion rate, however, in high voltage, increased duration decreases cleavage rate and blastocyst formation rate. Blastocyst formation rate in UCG showed a better development (32%) compared to FG (20%) or ECG (22.5%) (p<0.05).
Conclusion: It can be concluded that for optimal fusion, cleavage and development, one pulse of 0.75 kV/cm for 60μs should be applied.
Shahdokht Motazedian, Bahareh Hamedi, Jaleh Zolghadri, Khatereh Mojtahedi, Nasrin Asadi,
Volume 8, Issue 1 (7-2010)

Background: Although intrauterine insemination (IUI) is one of the most common methods which is used for male factor and unexplained infertility the relative influence of various semen parameters on the likelihood of a successful outcome is controversial. Several semen parameters have been evaluated as predictors of a successful outcome with intrauterine insemination.
Objective: To evaluate the effects of sperm morphology on the success rate of IUI.
Materials and Methods: This was a prospective study of 200 couples who underwent IUI cycles of ovarian stimulation in Shiraz University of Medical Sciences. The patients were chosen in 2 groups group A: 100 patients with ≤20% normal sperm morphology and group B: 100 patients with >20% normal sperm morphology (unexplained infertility). The other semen parameters were normal in both groups.
Results: Total clinical pregnancies were 10.5% (pregnancy rate / cycle). There was not any difference between two groups in rate of pregnancy and also pregnancy outcome.
Conclusion: Intrauterine insemination used for treating male factor infertility has not shown excessive advantage when normal sperm morphology in semen analysis is more than 20% in comparison with ≤ 20%.
Maryam Eidi, Akram Eidi, Omid Pouyan, Pouneh Shahmohammadi, Reza Fazaeli, Massih Bahar,
Volume 8, Issue 2 (7-2010)

Background: The trace element copper has been identified as a highly toxic element for sperm. It is known to affect sperm motility in humans and experimental implantation of copper in the epididymis vas deferens and scrotum of mammals has been demonstrated to affect fertility detrimentally. Objective: Sperm concentration motility vitality and morphology are parameters used to evaluate potential male fertility. Since copper is believed to be important for spermatogenesis; we conducted a study to investigate the correlation between seminal plasma copper concentration and human semen parameters in 232 males.
Materials and Methods: We selected 232 subfertile or infertile men who referred to Omid Fertility Clinic randomly. Samples were categorized into normospermic (n=32) oligozospermic (n=73) asthenozospermic (n=111) and azospermic (n=16) groups according to their spermiogrames. Total seminal plasma copper concentration was determined by furnace atomic absorption spectrophotometer.
Results: The results showed that seminal plasma copper concentrations in oligozospermic asthenozospermic and azospermic groups are significantly higher than normozospermic group (p<0.01). Also, negative correlations were found between seminal plasma copper concentration and sperm count (p<0.05), sperm motility (p<0.01), sperm vitality (p<0.01), normal morphology (p<0.01) and pH (p<0.01) in all groups.
Conclusion: It was suggested that excess copper in seminal plasma was detrimental for male reproductive capacity by reducing sperm count, motility, vitality and morphology.
Seyed Gholam Ali Jorsaraei, Hiroaki ShiBahara, Ayustawati , Yuki Hirano, Tatsuya Suzuki, Eisa Tahmasbpour Marzony , Mahtab Zainalzadeh, Mitsuaki Suzuki,
Volume 8, Issue 3 (7-2010)

Background: Leptin is a polypeptide hormone secreted by white adipose tissue in proportion to body energy. Although the participation of leptin in female reproduction is well established any role in male reproductive function is at best tenuous.
Objective: The objective of this study was to compare the leptin concentration in human seminal plasma and then the relationships between seminal leptin and semen parameters were evaluated.
Materials and Methods: Semen samples were provided from 71 men; normozoospermic (n=22) asthenozoospermic (n=31) and oligoasthenozoospermic (n=18) referring to Jichi Medical University Hospital for in vitro fertilization-embryo transfer (IVF-ET) treatment. After liquefaction all sperm specimens were evaluated for sperm parameters and motility characteristics by computer-assisted semen analysis (CASA) system. After semen analysis concentrations of leptin in seminal plasma of all groups were measured by ELISA.
Results: The mean concentrations of leptin in seminal plasma of normozoospermic asthenozoospermic and oligoasthenozoospermic men were 0.75+/-0.09 ng/ml 0.8+/-0.14 ng/ml and 0.8+/-0.15 ng/ml respectively. A trend was observed for a lower leptin concentration in seminal plasma of normozoospermic men compared with asthenozoospermic and oligoasthenozoospermic men. There was a significant negative correlation between seminal plasma leptin concentration with sperm motility (p<0.05) and Curvilinear Velocity of the sperm kinetic parameter (p<0.01).
Conclusion: It was demonstrated that there was a significant correlation between seminal leptin with the sperm motility.
Azam Azargoon, Raheb Ghorbani, Fereshteh AsleBahar,
Volume 14, Issue 5 (5-2016)

Background: Preterm birth is the major cause of neonatal mortality and morbidity.
Objective: The aim of this study was to evaluate the effect of prophylactic vaginal progesterone on decreasing preterm birth rate and neonatal complications in a high-risk population.
Materials and Methods: A randomized, double-blind, placebo-controlled study was performed on 100 high-risk singleton pregnancies. Vaginal suppository progesterone (400 mg) or placebo was administered daily between 16-22 wks to 36 wks of gestation. Progesterone (n=50) and placebo (n=50) groups were compared for incidence of preterm delivery and neonatal complications.
Results: The preterm birth rate was 52%. Preterm birth rate before the 37 wks of gestation (68% vs. 36%: RR=1.89, 95% CI: 1.25-2.86) and also before the 34 wks of gestation (42% vs. 18%: RR=2.33, 95% CI: 1.19-4.58) in placebo group was significantly higher than progesterone group. Our study also showed that the administration of vaginal progesterone was associated with a significant reduction in the risk of birth weight ≤2500 gr, the rates of respiratory distress syndrome (RDS) and admission to the Neonatal Intensive Care Unit (NICU) in the progesterone group when compared with the placebo group. However, there was no significant difference between the two groups in terms of neonatal death, days of admission in NICU, intraventricular hemorrhage and necrotizing enterocolitis.
Conclusion: Prophylactic vaginal progesterone reduced the rate of preterm delivery, the risk of a birth weight ≤2500 gr, the rates of RDS and admission to NICU in women who were at risk of preterm delivery.
Saeed Mirzaee, Mohammad Ehsan Bayatpoor, Shima Shahyad, Mohammad Taghi Mohammadi, Zahra Bahari,
Volume 17, Issue 2 (February 2019 2019)

Background: Male hypogonadism is associated with type II diabetes mellitus due to testicular dysfunction. Medicinal plants have received considerable attention for the management of diabetes and its complications.
Objective: The aim of present study was to evaluate the anti-diabetic and protective influence of Crocin on testopathy in diabetic rats.
Materials and Methods: In this experimental study, type II of diabetes mellitus was induced by high-fat diet and low dose of streptozotocin. Male Wistar rats (8 weeks,150–200 gr, 18 rats; n= 6 per group) were divided into a control group (standard diet), diabetic group (streptozotocin+high-fat diet), and treatment group (High-fat diet+streptozotocin+Crocin at 20 mg/kg/day, i.p. for 60 days). After 60 days, animals were euthanized, testis and epididymis were dissected, and weights of testes and sperm count were analyzed. Hematoxylin-eosin-stained was done for histopathological examination. Blood samples were collected for the assessment of serum glucose and cholesterol.
Results: High-fat diet and streptozotocin significantly increased the serum glucose and cholesterol levels as compared to the control group (p≤ 0. 001). Moreover, there was a significant decrease in the weight of right (p= 0.008) and left testes (p≤ 0.001) and also the total sperm count (p= 0.023) in the diabetic group compared with the control group. Current results also identified that type II diabetes mellitus induced degeneration in the morphology of seminiferous tubules. Application of Crocin couldsignificantly decrease serum glucose and cholesterol levels (p= 0.003). Furthermore, Crocin treatment significantly increased the weight of the right (p= 0.026) and left (p= 0.014) testes and total sperm count (p= 0.000). Also, Crocin could attenuate the
pathological changes of the testes in the treatment group.
Conclusion: Present findings concluded that Crocin treatment improved diabetic testopathy and impairment of seminiferous tubules induced by high-fat diet and streptozotocin.
Bahare Keshavarzi, Meraj Tabatabaei, Amir Hasan Zarnani, Fahime Ramezani Tehrani, Mahmood Bozorgmehr, Nariman. Mosaffa,
Volume 18, Issue 6 (June 2020)

Background: The amniotic membrane plays an important role in maintaining a healthy pregnancy. The main population cells from amniotic membrane include human amnion epithelial cells (hAECs) which have been shown to possess immunomodulatory properties.
Objective: The proximity of hAECs with monocyte leads to the generation of tollerogenic dendritic cells.
Materials and Methods: hAECs were obtained from normal pregnancy. Peripheral blood monocytes were isolated by anti-CD14 MACS method. Co-cultures of monocytes and hAECs were established in Transwell chambers supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) in the absence and presence of lipopolysaccharide (LPS) to produce immature and mature DCs, respectively. Immunophenotyping of the obtained DCs was done through flow cytometry and the production of cytokines was measured by ELISA. Mixed leukocyte Reaction (MLR) was also performed for the functional assessment of DCs.
Results: Immunophenotyping of [hAECs - Immature DC (iDC)] and [hAECs - iDC] + LPS cells revealed that the expression of CD1a, CD80, CD86, CD40, HLA-DR, and CD83 markers showed no significant difference as compared with the control group (iDCs and mDCs alone). In the [hAECs-iDCs] + LPS cells, the percentage of CD14 cells at the ratio of 1:2.5 showed significant differences compared to the control group. The production of IL-10 and IL-12 showed no significant difference in any of the cultures as compared to the control groups. Also, co-cultured DCs did not inhibit proliferation of lymphocyte.
Conclusion: Our findings show that factors secreted from cultured hAECs are unable to generate of tollerogenic dendritic cells. To achieve a better understanding of other mechanisms more investigations are needed.

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