Volume 15, Issue 10 (October 2017)                   IJRM 2017, 15(10): 649-660 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Monsefi M, Nadi A, Alinejad Z. The effects of Salvia officinalis L. on granulosa cells and in vitro maturation of oocytes in mice. IJRM. 2017; 15 (10) :649-660
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-872-en.html
1- Department of Biology, School of Sciences, Shiraz University, Shiraz, Iran. , monsefi@susc.ac.ir
2- Department of Biology, School of Sciences, Shiraz University, Shiraz, Iran
Abstract:   (90 Views)
Background: Salvia officinalis L. has been used since ancient times but there are little data about effects of this herb on normal reproductive cells. Objective: To investigate the toxicity effects of Salvia officinalis L. on granulosa cells (GCs) and maturation of oocytes. Materials and Methods: GCs and oocytes were extracted from superovulated ovaries of immature mice. The cells were treated with concentrations of 10, 50, 100, 500, and 1000 μg/ml of Salvia officinalis hydroalcoholic extracts and compared with the control culture. Bioviability, chromatin condensation, estradiol and progesterone concentrations, lipid synthesis, apoptosis, and alkaline phosphatase activity of GCs were measured. In vitro maturation of oocytes by determination of different maturation stages of oocytes including germinal vesicle, germinal vesicle breaks down, and metaphase II were examined. Results: The results revealed that 500 and 1000 μg/ml concentrations of Salvia officinalis L. were toxic. The most of the GCs were in the early stages of apoptosis in 100 μg/ml treated culture and cell death happened with 500 μg/ml treatment. Progesterone concentration was reduced in 100 μg/ml and higher doses but estradiol concentration and alkaline phosphatase showed opposite effects. The lipid droplets content of GCs reduced significantly in all groups especially in 500 and 1000 μg/ml. Finally, oocyte’s nucleus and cytoplasm showed a high level of condensation, and meiosis rate reduced in all treated cultures. Conclusion: Our findings suggested that higher dose of Salvia officinalis hydroalcoholic extracts inhibits, oocyte maturation, GCs bioviability, proliferation, and secretion
Full-Text [PDF 695 kb]   (60 Downloads)    
Type of Study: Original Article |
Received: 2017/12/4 | Accepted: 2017/12/4 | Published: 2017/12/4

References
1. Khalil R, Li ZG. Antimicrobial activity of essential oil of Salvia officinalis L. collected in Syria. Afr J Biotechnol 2011; 10: 8397-8402. [DOI:10.5897/AJB10.2615]
2. Walch SG, Tinzoh LN, Zimmermann BF, Stühlinger W, Lachenmeier DW. Antioxidant capacity and polyphenolic composition as quality indicators for aqueous infusions of Salvia officinalis L. (sage tea). Front Pharmacol 2011; 2: 79. [DOI:10.3389/fphar.2011.00079]
3. Khan A, Rehman NU, AlKharfy KM, Gilani AH. Antidiarrheal and antispasmodic activities of Salvia officinalis are mediated through activation of K+ channels. Bangladesh J Pharmacol 2011; 6: 111-116. [DOI:10.3329/bjp.v6i2.9156]
4. Ayatollahi SA, Shojaii A, Kobarfard F, Mohammadzadeh M, Choudhary MI. Two flavones from Salvia leriaefolia. Iran J Pharm Res 2009; 8: 179-184.
5. Hamidpour M, Hamidpour R, Hamidpour S, Shahlari M. Chemistry, pharmacology, and medicinal property of sage (salvia) to prevent and cure lllnesses such as obesity, diabetes, depression, dementia, lupus, autism. heart disease, and cancer. J Tradit Complement Med 2014; 4: 82-88. [DOI:10.4103/2225-4110.130373]
6. Hadri AE, Gó mez del Rí o MA, Sanz J, Gonzá lez Coloma A, Idaomar M, Ribas Ozonas B, et al. Cytotoxic activity of á-humulene and transcaryophyllene from salvia officinalis in animal and human tumor cells. An R Acad Nac Farm 2010; 76: 343-356.
7. Jedinak A, Muckova M, Kostalova D, Maliar T, Masterova I. Antiprotease and antimetastatic activity of ursolic acid isolated from salvia officinalis. Z Naturforsch C 2006; 61: 777-782. [DOI:10.1515/znc-2006-11-1203]
8. Deans SG, Simpson EJM. Antioxidants from Salvia officinalis. En: The Genus Salvia. Amsterdam: Kintzios; 2000. 185-192.
9. Lu Y, Foo LY. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 2001; 75: 197-202. [DOI:10.1016/S0308-8146(01)00198-4]
10. Monsefi M, Abedian M, Azarbahram Z, Ashraf MJ. Salvia officinalis L. Induces alveolar bud growing in adult female rat mammary glands. Avicenna J Phytomed 2015; 5: 560-567.
11. Sohrabi M, Mohammadi Roushandeh A, Alizadeh Z, Vahidinia A, Vahabian M, Hosseini. Effect of a high fat diet on ovary morphology, in vitro development, in vitro fertilisation rate and oocyte quality in mice. Singapore Med J 2015; 56: 573-579. [DOI:10.11622/smedj.2015085]
12. Dadoune JP, Mayaux MJ, Guihard-Moscato ML. Correlation between defects in chromatin condensation of human spermatozoa stained by aniline blue and semen characteristics. Andrologia 1988; 20: 211-217. [DOI:10.1111/j.1439-0272.1988.tb01058.x]
13. Kasibhatla S, Amarante-Mendes GP, Finucane D, Brunner T, Bossy-Wetzel E, Green DR. Acridine orange/ethidium bromide (AO/EB) staining to detect apoptosis. CSH Protoc 2006; 2006: pii: pdb.prot4493.
14. Wang H, Wang H, Xiong W, Chen Y, Ma Q, Ma J, et al. Evaluation on the phagocytosis of apoptotic spermatogenic cells by sertoli cells in vitro through detecting lipid droplet formation by Oil Red O staining. Reproduction 2006; 132: 485-492. [DOI:10.1530/rep.1.01213]
15. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007; 35: 495-516. [DOI:10.1080/01926230701320337]
16. Roby MHH, Sarhan MA, Selim KAH, Khalel KI. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Ind. Crops Prod 2013; 43: 827-831. [DOI:10.1016/j.indcrop.2012.08.029]
17. Ososki AL, Kennelly EJ. Phytoestrogens: a review of the present state of research. Phytother Res 2003; 17: 845-869. [DOI:10.1002/ptr.1364]
18. Nynca A, Jablonska O, Slomczynska M, Petroff BK, Ciereszko RE. Effects of phytoestrogen daidzein and estradiol on steroidogenesis and expression of estrogen receptors in porcine luteinized granulosa cells from large follicles. J Physiol Pharmacol 2009; 60: 95-105.
19. Nynca A, Nynca J, Wasowska B, Kolesarova A, Kołomycka A, Ciereszko RE. Effects of the phytoestrogen, genistein, and protein tyrosine kinase inhibitor–dependent mechanisms on steroidogenesis and estrogen receptor expression in porcine granulosa cells of medium follicles. Domest Anim Endocrinol 2013; 44: 10-18. [DOI:10.1016/j.domaniend.2012.07.002]
20. Tiemann U, Schneider F, Vanselow J, Tomek W. In vitro exposure of porcine granulosa cells to the phytoestrogens genistein and daidzein: effects on the biosynthesis of reproductive steroid hormones. Reprod Toxicol 2007; 24: 317-325. [DOI:10.1016/j.reprotox.2007.07.008]
21. Pozo-Guisado E, Merino JM, Mulero-Navarro S, Lorenzo-Benayas MJ, Centeno F, Alvarez-Barrientos A, et al. Resveratrol-induced apoptosis in MCF‐7 human breast cancer cells involves a caspase-independent mechanism with downregulation of Bcl‐2 and NF-κB. Int J Cancer 2005; 115: 74-84. [DOI:10.1002/ijc.20856]
22. Stochmalova A, Kadasi A, Alexa R, Sirotkin A. Plant molecules quercetin and resveratrol can affect ovarian cells and invert FSH action. Endocrine Abstracts 2014; 34: 318. [DOI:10.1530/endoabs.34.P318]
23. Xavier CP, Lima CF, Fernandes-Ferreira M, Pereira-Wilson C. Salvia fruticosa, salvia officinalis, and rosmarinic acid induce apoptosis and inhibit proliferation of human colorectal cell lines: the role in MAPK/ERK pathway. Nutr Cancer 2009; 61: 564-571. [DOI:10.1080/01635580802710733]
24. Behroozi Moghadam S, Masoudi R, Monsefi M. Salvia officinalis L. induces apoptosis in mammary carcinoma cells through alteration of Bax to Bcl-2 ratio. Iran J Sci Technol 2016; 40.
25. Fukushima M, Tanaka S, Sato T, Hashimoto M. Morphological and biochemical studies on cultured human granulosa cells. Asia Oceania J Obstet Gynaecol 1983; 9: 473-479. [DOI:10.1111/j.1447-0756.1983.tb00661.x]
26. Adlercreutz H, Bannwart C, Wahala K, Makela T, Brunow G. Hase T. Inhibition of human aromatase by mammalian lignans and isoflavonoid phytoestrogens. J Steroid Biochem Mol Biol 1993; 44: 147-153. [DOI:10.1016/0960-0760(93)90022-O]
27. Santini SE, Basini G, Bussolati S, Grasselli F. The phytoestrogen quercetin impairs steroidogenesis and angiogenesis in swine granulosa cells in vitro. J Biomed Biotechnol 2009; 2009: 419891. [DOI:10.1155/2009/419891]
28. Bucci M, Murphy CR. Hormonal control of enzyme activity during the plasma membrane transformation of uterine epithelial cells. Cell Biol Int 2001; 25: 859-871. [DOI:10.1006/cbir.2001.0771]
29. Niknafs B, Afshar F, Dezfulian AR. The effects of different luteal support hormones on endometrial alkaline phosphatase activity and endometrial thickness in superovulated mice. Iran J Reprod Med 2010; 8: 18-23.
30. Janne OA. Progesterone action in mammalian uterus. Acta Obstet Gynecol Scand 1981; 101 (Suppl): 11-16. [DOI:10.3109/00016348109157805]
31. Wober J, Weisswange I, Vollmer G. Stimulation of alkaline phosphatase activity in Ishikawa cells induced by various phytoestrogens and synthetic estrogens. J Steroid Biochem Mol Biol 2002; 83: 227-233. [DOI:10.1016/S0960-0760(02)00252-2]
32. Yoshida N, Mizuno K. Effect of physiological levels of phytoestrogens on mouse oocyte maturation in vitro. Cytotechnol 2012; 64: 241-247. [DOI:10.1007/s10616-011-9369-2]
33. Hoskova K, Krivohlavkova L, Kadleckova L, Rajmon R, Drabek O, Jílek F. Biochanin a and daidze in influence meiotic maturation of pig oocytes in a different manner. Sci Agric Bohemica 2014; 45: 155-161.
34. Kang JT, Kwon DK, Park SJ, Kim SJ, Moon JH, Koo OJ, et al. Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels. J Vet Sci 2013; 14: 15-20. [DOI:10.4142/jvs.2013.14.1.15]
35. Orlovschi D, Miclea I, Zahan M, Miclea V, Pernes AJ. Quercetin efficacy on in vitro maturation of porcine oocytes. Anim Sci Biotechnol 2014; 47: 113-115.
36. Liu Y, He XQ, Huang X, Ding L, Xu L, Shen YT, et al. Resveratrol protects mouse oocytes from methylglyoxal-induced oxidative damage. PLoS One 2013; 8: e77960. [DOI:10.1371/journal.pone.0077960]
37. Mohaisen HA, Saad SA, Ferial KK. Effect of aqueous extract of medicago sativa and salvia officinalis mixture on hormonal, ovarian and uterine parameters in mature female mice. J Mater Environ Sci 2013; 4: 424-433.

Send email to the article author


© 2015 All Rights Reserved | International Journal of Reproductive Biomedicine

Designed & Developed by : Yektaweb