Volume 12, Issue 10 (11-2014)                   IJRM 2014, 12(10): 695-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tumkiratiwong P, Ploypattarapinyo R, Pongchairerk U, Thong-asa W. Reproductive toxicity of Momordica charantia ethanol seed extracts in male rats. IJRM. 2014; 12 (10) :695-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-486-en.html
1- Physiology Division, Department of Zoology, Faculty of Sciences, Kasetsart University, Bangkok, 10900, Thailand , fscipnt@ku.ac.th
2- Physiology Division, Department of Zoology, Faculty of Sciences, Kasetsart University, Bangkok, 10900, Thailand
3- Department of Anatomy, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand
Abstract:   (148 Views)
Background: Momordica charantia (M. charantia) seed has been supposed to have an antifertility property but mechanisms underlying the infertility effect have not been investigated.
Objective: We investigated the antifertility effect of M. charantia ethanol seed extracts on reproductive toxicology and seminal and plasma testosterone in male Wistar rats.
Materials and Methods: The control group (I) was provided daily 1 ml dimethylsulfoxide (DMSO) and the experimental groups II and III were given daily 400 and 800 mg dry matter/kg body weight of the extracts dissolved in 1 ml DMSO via the esophageal route. All groups were administered for 42 days (day 42). Changes in body weight, fertility, reproductive characteristics, testicular histopathology and levels of seminal and plasma testosterone among three groups were compared.
Results: On day 42, the extracts caused antifertility (p=0.001). The extracts demonstrated significant reductions in diameters of seminiferous tubules and epididymides, spermatid density, daily sperm production and caudal epididymal spermatozoa, sperm motility and viability (p=0.046). Pathological changes in seminiferous tubules revealed atrophy, desquamation, pyknosis nucleus and multinucleated giant cell. Plasma cells were evident in three parts of epididymides of rats treated with high dose of the extract. Furthermore, the high dose of the extract suppressed seminal testosterone level (p=0.001) and plasma testosterone level (p=0.002).
Conclusion: Our data showed that high dose of M. Charantia seed extracts caused infertility in male rats. The interruption in their fertility was probably attributed to the direct toxic to seminiferous tubules, epididymis and the lowered testosterone level which might impact on sperm parameters.
Full-Text [PDF 494 kb]   (77 Downloads) |   |   Full-Text (HTML)  (8 Views)  
Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2018/02/19 | Published: 2018/02/19

1. Mosher WD, Pratt WF. Fecundity and infertility in the United States: incidence and trends. J Fertil Steril 1991; 56: 192-193.
2. Dehghan MH, Martin T, Dehghanan R. Antifertility effect of Iranian neem seed alcoholic extract on epididymal sperm of mice. Iran J Reprod Med 2005; 3: 83-89.
3. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal plants. Central drug research institute, Lucknow and publications and information directorate. New Delhi, India; 1993.
4. Scartezzini P, Speroni E. Review on some plants of Indian traditional medicine with antioxidant activity. J Ethnopharmacol 2000; 71: 23-43. [DOI:10.1016/S0378-8741(00)00213-0]
5. Naseem MZ, Patil SR, Ravindra SR, Patil RS. Antispermatogenic and androgenic activities of Momordica charantia (Karela) in albino rats. J Ethnopharmacol 1998; 61: 9-16. [DOI:10.1016/S0378-8741(98)00006-3]
6. Patil SA, Patil SB. Toxicological studies of Momordica charantia Linn. Seed extracts in male mice. Int J Morphol 2011; 29: 1212-1218. [DOI:10.4067/S0717-95022011000400024]
7. Yama OE, Osinubi AA, Duru FIO, Noronha CC, Okanlawon AO. Contraceptive effect of methanolic extract of Momordica charantia seed in male Sprague Dawley rats. Asian J Pharm Clin Res 2011; 4: 22-26.
8. Sharanabasappa A, Vijaykumar B, Saraswati BP. Effect of Momordica charantia seed extracts on ovarian and uterine activities in Albino rats. Pharm Biol 2002; 40: 501-507. [DOI:10.1076/phbi.40.7.501.14688]
9. Abalaka ME, Olonitola OS, Onaolapo JA, Inabo HI. Evaluation of acute toxicity of Momordica charantia extract using Wistar Rats to determine safety levels and usefulness of the plant in ethnochemotherapy. Int J P App Sci 2009; 3: 1-6.
10. World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. 3rd Ed. Cambridge University Press, Cambridge; 1999.
11. Amann RP, Howards SS. Daily spermatozoal production and epididymal spermatozoal reserves of the human male. J Urol 1980; 124: 211-215. [DOI:10.1016/S0022-5347(17)55377-X]
12. Luna LG. Manual of Histological Staining Methods of the Armed Force Institute of Pathology. McGraw-Hill Book Company, New York; 1968.
13. Linares V, Albina ML, Belles M, Mayayo E, Sanchez DJ, Domingo JL. Combined action of uranium and stress in the rat. II. Effects on male reproduction. Toxicol Lett 2005; 158: 186-195. [DOI:10.1016/j.toxlet.2005.03.015]
14. Abul HT, Mathew TC, Abul F, Al-Sayer H, Dashti HM. Antioxidant enzyme level in the testes of cirrhotic rats. Nutrition 2002; 18: 56-59. [DOI:10.1016/S0899-9007(01)00743-2]
15. Gupta RS, Kachhawa JB, Chaudhary R. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats. Asian J Androl 2004; 6: 155-159.
16. Seetharam YN, Sujeeth H, Jyothishwaran G, Barad A, Sharanabasappa G, Umareddy B, Vijaykumar MB, Patil SB. Antifertility effect of ethanolic extract of Amalakyadi churna in male Albino mice. Asian J Androl 2003; 5: 247-250.
17. Bhargava SK. Antifertility agents from plants. Fitoterapia 1988; 59: 163-177.
18. Lohiya NK, Manivannan B, Mishra PK, Pathak N, Sriram S, Bhande SS, Panneerdoss S. Chloroform extract of Carica papaya seeds induces long-term reversible azoospermia in langur monkey. Asian J Androl 2002; 4: 17-26.
19. Parandin R, Sadeghipour HR, Haeri Rohani SA. Evaluation of antifertility effect and recovery of the seed oil constituents of Iranian species of Melia azadrach L. in male rats. J Reprod Contracept 2008; 19: 161-166. [DOI:10.1016/S1001-7844(08)60019-X]
20. Tumkiratiwong P, Lerkchundhakriat K. Effect of a pyrimethamine-sulfanilamide combination on induced temporal infertility in male Wistar Rats. Kasetsart J (Nat Sci) 2011; 45: 59-69.
21. Goyal HO, Braden TD, Mansour M, Williams CS, Kamaleldin A, Srivastava KK. Diethylstilbestrol-treated adult rats with altered epididymal sperm numbers and sperm motility parameters, but without alterations in sperm production and sperm morphology. Biol Reprod 2001; 64: 927-934. [DOI:10.1095/biolreprod64.3.927]
22. Garrett C, Liu DY, Clarke GN, Rushford DD, Baker HW. Automated semen analysis: zona pellucida preferred sperm morphometry and straight-line velocity are related to pregnancy rate in subfertile couples. Hum Reprod 2003; 18: 1643-1649. [DOI:10.1093/humrep/deg306]
23. Liu DY, Garrett C, Baker HW. Low proportions of sperm can bind to the zona pellucida of human oocytes. Hum Reprod 2003; 18: 2382-2389. [DOI:10.1093/humrep/deg456]
24. Auger J. Assessing human sperm morphology: top models, underdogs or biometrics? Asian J Androl 2010; 12: 36-46.
25. Shetty AJ, Narayana K. The effects of carbamazepine on sperm morphology in Wistar rats. Indian J Physiol Pharmacol 2007; 51: 255-260.
26. Mohan J, Panda JN, Singh US, Moudgal RP. Studies on antifertility effects of gossypol acetic acid in domestic cocks. J Reprod Fertil 1989; 85: 73-78. [DOI:10.1530/jrf.0.0850073]
27. Damjanov I. High-Yield Pathology. 2PndP Ed. Lippincot Williams and Wilkins, Baltimore; 2005.
28. Phadke AM, Phadke GM. Occurrence of macrophage cells in the semen and in the epididymis in case of male infertility. J Reprod Fertil 1961; 2: 400-403. [DOI:10.1530/jrf.0.0020400]
29. Mishra N, Joshi S, Tandon VL, Munjal A. Evaluation of anti-fertility potential of aqueous extract of Bougainvillea spectabilis leaves in Swiss Albino mice. IJPSDR 2009; 1: 19-23.
30. Hikim AP, Lue YH, Wang C, Reutrakul V, Sangsuwan R, Swerdloff RS. Posttesticular antifertility action of triptolide in the male rat: evidence for severe impairment of cauda epididymal sperm ultrastructure. J Androl 2000; 21: 431-437.
31. Chaturvedi M, Mali PC, Ansari AS. Induction of reversible antifertility with a crude ethanol extract of Citrullus colocynthis Schrad fruit in male rats. Pharmacology 2003; 68: 38-48. [DOI:10.1159/000068727]
32. Luangpirom A, Sirisarn W, Pontaisog J. Antifertility activity of the aqueous leaf extract of Cissampelos pareira in male Albino mice. ABAH Bioflux 2010; 2: 59-64.
33. Frayne J, Hall L. The potential use of sperm antigen as targets for immunocontraception: past, present and future. J Reprod Immunol 1999; 43: 1-33. [DOI:10.1016/S0165-0378(99)00005-4]
34. de Kretser DM, Huidobro C, Southwick GJ, Temple-Smith PD. The role of the epididymis in human infertility. J Reprod Fertil 1998; 53: 271-275.
35. Odusoga AO, Ifabunmi OO, Ayokunle O. Evaluation of oral administration of aqueous leaf extract of Momordica charantia on fertility hormones of adult male Wistar rats. Glob J Pharmacol 2014; 8: 150- 153.
36. Turner TT, Jones CE, Howards SS, Ewing LL, Zegeye B, Gunsalus GL. On the androgen microenvironment of maturing spermatozoa. Endocrinology 1984; 115: 1925-1932. [DOI:10.1210/endo-115-5-1925]
37. Zirkin BR, Santulli R, Awoniyi CA, Ewing LL. Maintenance of advanced spermatogenic cells in the adult rat testis: quantitative relationship to testosterone concentration within the testis. Endocrinology 1989; 124: 3043-3049. [DOI:10.1210/endo-124-6-3043]
38. Creasy DM. Pathogenesis of male reproductive toxicity. Toxicol Pathol 2001; 29: 64-76. [DOI:10.1080/019262301301418865]
39. Dohle GR, Smit M, Weber RFA. Androgen and male fertility. World J Urol 2003; 21: 341-345. [DOI:10.1007/s00345-003-0365-9]
40. Cooper TG. The epididymis as a site of contraceptive attack. In: Nieschlag E, Habenicbt UF (eds.). Spermatogenesis, fertilization and contraception. Springer, Berlin; 1992.

Add your comments about this article : Your username or Email:

Send email to the article author

© 2019 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb