Volume 3, Issue 2 (7-2005)                   IJRM 2005, 3(2): 79-0 | Back to browse issues page

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Karimpor Malekshah A, Esmailnejad Moghaddam A. The effect of culture medium volume on in vitro development of mouse embryos. IJRM. 2005; 3 (2) :79-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-36-en.html
Abstract:   (141 Views)
Background: In the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. A smaller medium drop can prevent dilution of this factor(s). Objective: The objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. Materials and Methods: The embryos were obtained from female NMRI mice. To evaluate the effect of medium volume, groups of 16-20 late 2-cell embryos were cultured in 2, 5, 10, 20, 50 and 100 �l of drops of Ham�s F10 medium for 72 h. Results: Development to blastocyst stage in 50 and 100 �l of drop were significantly higher than this in any other volume (p<0.001). Almost a similar pattern was also observed for hatched blastocyst formation. However, the total number of cells in blastocysts, developing in different volumes, were not significantly different. Conclusion: These results indicate that the optimal volumes of Ham�s F10 medium for mouse early embryo development are 50 to 100 �l. However, volumes as small as 2 �l can successfully support mouse 2-cell embryo development to blastocyst and hatching stages.
Full-Text [PDF 89 kb]   (99 Downloads)    
Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2017/10/5 | Published: 2017/10/5

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