Volume 11, Issue 1 (4-2013)                   IJRM 2013, 11(1): 53-0 | Back to browse issues page

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Mangoli E, Talebi A R, Anvari M, Pourentezari M. Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice. IJRM. 2013; 11 (1) :53-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-343-en.html
1- Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Iran
2- Department of Andrology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Iran , prof_talebi@ssu.ac.ir
Abstract:   (144 Views)
Background: Diabetes mellitus (DM), primary or idiopathic is a chronic disorder of the carbohydrate, lipid and protein metabolism. DM may impact male reproductive function at several levels. It is shown that DM has detrimental effects on sperm parameters in human and experimental animals.
Objective: The aim of this study was to observe the effects of diabetes on sperm parameters (viability, count, morphology and motility) and evaluation of sperm chromatin quality in mice.
Materials and Methods: Totally twenty adult male Syrian mice were divided randomly into 2 groups (n=10). The animals of group A were considered as controls while group B mice were diabetic that received a single dose (200 mg/kg) streptozotocin (STZ) intra peritoneally. After 35 days, the cauda epididymis of each diabetic mouse was dissected and placed in culture medium for 30 min. The swim-out spermatozoa were analyzed for count, motility, morphology and viability. The sperm chromatin quality and DNA integrity, was evaluated with Aniline Blue (AB), Toluidine blue (TB), Acridine orange (AO) and Chromomycin A3 (CMA3) staining.
Results: In sperm analysis, the diabetic mice had poor parameters in comparison with control animals (p=0.000). Regarding sperm chromatin quality, the results of TB and AO tests showed statically significant differences between two groups, but in AB and CMA3 staining, we didn’t see any differences between them.
Conclusion: The results showed that STZ-induced diabetes mellitus may influence the male fertility potential via affecting sperm parameters and DNA integrity in mice. However, according to our data, the diabetes doesn’t have any detrimental effects on histone-protamines replacement during the testicular phase of sperm chromatin packaging.
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Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2018/02/24 | Published: 2018/02/24

References
1. Petroianu A, Alberti LR, Antonio M, de Melo B, de Almeida LM. Relation between diabet mellitos and male fertility. Einstein 2009; 7: 407-410.
2. World Health Organization (WHO). Prevalence of diabetes worldwide-August 20th, 2007. Available in: http://www.who.int/diabetes/facts/world_figures/en/.
3. Agbaje IM, Rogers DA, McVicar CM, McClure N, Atkinson AB, Mallidis C, et al. Insulin dependent diabetes mellitus: implications for male reproductive function. Hum Reprod 2007; 22: 1871-1877. [DOI:10.1093/humrep/dem077]
4. Muralidhara Sh. Early oxidative stress in testis and epididymal sperm in streptozotocin- induce diabetic mice: its progression and genotoxic consequences. Report toxical 2007; 23: 578-587. [DOI:10.1016/j.reprotox.2007.02.001]
5. O'Neill J, Czerwiec A, Agbaje I, Glenn J, Stitt A, McClure N, et al. Differences in mouse models of diabetes mellitus in studies of male reproduction. Int J Androl 2010; 33: 709-716. [DOI:10.1111/j.1365-2605.2009.01013.x]
6. Ricci G, Catizone A, Esposito R, Pisanti FA, Vietri MT, Galdieri M. Diabetic rat testes: morpholigical and functional alterations. Andrologia 2009; 41: 361-368. [DOI:10.1111/j.1439-0272.2009.00937.x]
7. Joao RS, Amaral S, Oliveira P. Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive species. Bentham science 2009; 4: 1573-3993.
8. Talebi AR, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Dehghani Firoozabadi R, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. J Androl 2012; 44 (Suppl.): 462-470.
9. Talebi AR. Sperm nuclear maturation.1thEd. Nova science publishers, Inc. New York; 2011.
10. Talebi AR, Khalili MA, Nahangi H, Abbasi A, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat. Iran J Reprod Med 2006; 5: 171-176.
11. Carrell DT, Emery BR, Hammoud S .Altered protamine expression and diminished spermatogenesis: What is link? Hum Reprod Update 2007; 13: 313-327.
12. Talebi AR, Sarcheshmeh AA, Khalili MA, Tabibnejad N. Effect of ethanol consumption on chromatin condensation and DNA integrity of epididymal spermatozoa in rat. Alcohol 2011; 45: 403-409. [DOI:10.1016/j.alcohol.2010.10.005]
13. Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245-251. [DOI:10.1111/j.1439-0272.2008.00852.x]
14. Lolis D, Georgiou I, Syrrou M, Zikopoulos K, Konstantelli M, Messinis I. Chromomycin A3-staining as an indicator of protamine deficiency and fertilization. Int J Androl 1996; 19: 23-27. [DOI:10.1111/j.1365-2605.1996.tb00429.x]
15. Ficher M, Zuckerman M, Fishkin RE, Goldman A, Neeb M, Fink PJ, et al. Do endocrines play an etiological role in diabetic and non-diabetic sexual dysfunction? J Androl 1984; 5: 8-16.
16. Hassan AA, Hassouna MM, Taketo T, Gagnon C, Elhilali MM. The effect of diabetes on sexual behavior and reproductive tract function in male rats. J Urol 1993; 149: 148-154. [DOI:10.1016/S0022-5347(17)36028-7]
17. Arikawe AP, Daramola AO, Odofin AO, Obika LF. Alloxan-induced and insulin resistant diabetes mellitus affect semen parameters and impair spermatogenesis in male Rats. Reprod Health 2006; 10: 106-113.
18. La Vignera S, Condorelli R, Vicari E, D'Agata R, Calogero AE. Diabetes and sperm parameters:a brief review. J Androl 2011; 33: 145-153. [DOI:10.2164/jandrol.111.013193]
19. Sakkas D, Moffatt O, Manicardi GC, Mariethoz E, Tarozzi N, Bizzaro D. Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol Reprod 2002; 66: 1061-1067. [DOI:10.1095/biolreprod66.4.1061]
20. Steger RW, Rabe MB: The effect of diabetes mellitus on endocrine and reproductive function. Proc Soc Exp Biol Med 1997; 214: 1-11. [DOI:10.3181/00379727-214-44064]
21. Mahesh T, Menon VP. Quercetin alleviates oxidative stress in streptozotocin-induced diabetic rats. J Phytother Res 2004; 18: 123-127. [DOI:10.1002/ptr.1374]
22. Kartikeya M, Agarwal A, Sharma R. Oxidative stress and male infertility. Indian J Med Res 2009; 129: 357-367.
23. Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. Br J Urol Int 2005; 95: 503-507. [DOI:10.1111/j.1464-410X.2005.05328.x]
24. Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl 2005; 26: 654-660.

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