Volume 11, Issue 1 (4-2013)                   IJRM 2013, 11(1): 61-0 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Teremmahi Ardestani M, Hadi Nodushan H, Aflatoonian A, Ghasemi N, Sheikhha M H. Case control study of the factor V Leiden and factor II G20210A mutation frequency in women with recurrent pregnancy loss. IJRM. 2013; 11 (1) :61-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-334-en.html
1- Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Iran
2- Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Iran
3- Department of Medical Genetics, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Iran , n479g@yahoo.co.uk
4- Department of Medical Genetics, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Iran
Abstract:   (129 Views)
Background: Recurrent pregnancy loss (RPL) caused by various genetic and non-genetic factors. After chromosome abnormality, thrombophilia is one of the most important genetic factors that could cause RPL. Factor V Leiden and factor II G20210A mutation were the most common mutations cause thrombophilia in the world.
Objective: The purpose of this study was to determine the frequency of factor V Leiden and prothrombine gene mutations in women with RPL compared with women who had uneventful pregnancies.
Materials and Methods: This case control study evaluates the frequency of factor V-Leiden and factor II G20210 genotypes in 80 women with two or more pregnancy losses, compared with 80 women without adverse pregnancy outcome. The mutations were assessed by PCR-RFLP.
Results: Frequency of the factor V Leiden among cases was 2.5%, which was higher than controls (1.25%), but the difference was not significant. No factor II G20210 mutation was found among cases and controls.
Conclusion: These data did not confirm that factor V Leiden and factor II G20210 mutation might play a role in recurrent pregnancy loss in Iranian women.
Full-Text [PDF 252 kb]   (32 Downloads) |   |   Full-Text (HTML)  (3 Views)  
Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2018/02/24 | Published: 2018/02/24

References
1. Lee RM, Silver RM. Recurrent pregnancy loss: Summery and clinical recommendations. Semin Repord Med 2000; 18: 433-440. [DOI:10.1055/s-2000-13733]
2. Carrington B, Sacks G, Regan L. Recurrent miscarriage: pathophysiology and outcome. Curr Opin Obstet Gynecol 2005; 17: 591-597. [DOI:10.1097/01.gco.0000194112.86051.26]
3. Greer IA. Thrombophilia: Implications for pregnancy outcome. Thromb Res 2003; 109: 73-81. [DOI:10.1016/S0049-3848(03)00095-1]
4. Alfirevic Z, Roberts D, Martlew V. How strong is the association between maternal thrombophilia and adverse pregnancy outcome? A systemic review. Eur J Obstet Gynecol Reprod Biol 2002; 101: 6-14. [DOI:10.1016/S0301-2115(01)00496-1]
5. Taher A, Khalil I, Abou-Mehri R, Shamseddine A, Bazarbachi A. High prevalence of prothrombine G20210A mutation among patients with deep venous thrombosis in lebanon. Thromb Haemost 2003; 89: 945-946.
6. Irani-Hakim N, Tamim H, Kreidy R, Almai WY. The prevalence factor V R506Q mutation among apparently healthy lebanenes. Am J Hematol 2000; 65: 45-49. https://doi.org/10.1002/1096-8652(200009)65:1<45::AID-AJH8>3.0.CO;2-V [DOI:10.1002/1096-8652(200009)65:13.0.CO;2-V]
7. Bertina RM, Koeleman BP, Koster T, Rosendaal FR, Dirven RJ, de Ronde H, et al. Mutation in blood coagulation factor v associated with resistance to activated protein C. Nature 1994; 369: 64-67. [DOI:10.1038/369064a0]
8. Poort SR, Rosendaal FR, Reitsma PH. A common genetic variation in the 3′-UTR of the prothrombine gene is associated with elevated plasma prothrombine levels and an increase in venous thrombosis. Blood 1996; 88: 3698-3703.
9. Zeinali S, Duca F, Zarbakhsh B. Thrombophilic mutations in Iran. Thromb Haemost 2000; 83: 351-352.
10. Sanjary Sharma, Sirish I Cumar, Ujjal Podar. Factor V Leiden and factor IIG20210A mutations are uncommon in portal vein thrombosis in India. Ind Journal Of Gastoenterology 2006: 236-239.
11. O'Donnell J, Tuddenham EG, Manning R, Kemball-Cook G, Johnson D, Laffan M. High prevalence of elevated factor VIII levels in patients referred for thrombophilia screening: Role of increased synthesis and relationship to the acute phase reaction. Thromb Haemost 1997; 775: 825.
12. Van Tilburg NH, Rosendaal FR, Bertina RM. Thrombin activatable fibrinolysis inhibitor and the risk for deep vein thrombosis. Blood 2000; 95: 2855.
13. Kupferminc MJ, Eldor A, Steinman N, Many A, Bar-Am A, Jaffa A, et al. Increased frequency of genetic thrombophilia in women with complications of pregnancy. N Engl J Med 1999; 340:9. [DOI:10.1056/NEJM199901073400102]
14. Miller SA, Dykes D, Polesky HF. Asimple salting out procedure for extracting DNA from human nucleated cells. NUCL Acid Res 1998; 16: 1215. [DOI:10.1093/nar/16.3.1215]
15. O'Donnell J, Tuddenham EG, Manning R, Kemball-Cook G, Johnson D, Laffan M. High prevalence of elevated factor VIII levels in patients referred for thrombophilia screening: Role of increased synthesis and relationship to the acute phase reaction. Thromb Haemost 1997; 775: 825.
16. Van Tilburg NH, Rosendaal FR, Bertina RM. Thrombin activatable fibrinolysis inhibitor and the risk for deep vein thrombosis. Blood 2000; 95: 2855.
17. Souza RA, Ferriani AG. Pontes: Factor V Leiden and factor II G20210A utations in patients with recurrent abortion. Hum Reprod 1999; 14: 2448-2450. [DOI:10.1093/humrep/14.10.2448]
18. Glueck CJ, Wang P, Goldenberg N. Pregnancy loss, plysyctic ovary syndrome, thrombophilia, hypofibrinolysis, enoxaparin, metformin. Clin Appl Thromb Hemost 2004; 10: 323-334. [DOI:10.1177/107602960401000404]
19. Hashimoto K, Shizusawa Y, Shimoya K. The factor V leiden mutation in Japanese couples with recurrent spontaneous abortion. Hum Repord 1999; 14: 1872-1874. [DOI:10.1093/humrep/14.7.1872]
20. Behjati R, Modarressi MH. Thrombophilic mutations in Iranian patients with infertility and recurrent spontaneous abortion. Ann Hematol 2006; 85: 268-271. [DOI:10.1007/s00277-005-0021-0]
21. Akar N, Akar E, Dalgin G, Sozouz A, Omurlu K, Cin S. Frequency of factor v leiden mutation in Turkish population. Thromb Haemost 1997; 78: 1527-1528.
22. Dashti AA, Jadaon MM. Race differences in the prevalence of the factor V Leiden mutation in Kuwaiti nationals. Mol Biol Rep 2011; 38: 3623-3628. [DOI:10.1007/s11033-010-0474-7]

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2018 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb