Volume 10, Issue 2 (7-2012)                   IJRM 2012, 10(2): 121-0 | Back to browse issues page

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Najafi T, Ghaffari Novin M, Pakravesh J, Foghi K, Fadayi F, Rahimi G. Immunohistochemical localization of endothelial nitric oxide synthase in endometrial tissue of women with unexplained infertility. IJRM. 2012; 10 (2) :121-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-263-en.html
1- Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran
2- Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran , mghaffarin@yahoo.com
3- Infertility Treatment Center, Aban Hospital, Tehran, Iran, Iran
4- Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Iran
Abstract:   (126 Views)
Background: Nitric oxide (NO) is a molecule that incorporates in many physiological processes of female reproductive system. Recent studies suggested the possible role of endothelial isoform of nitric oxide synthase (eNOS) enzyme in female infertility.
Objective: The aim of this study is to evaluate the expression of endothelial nitric oxide synthase in endometrial tissue of women with unexplained infertility.
Materials and Methods: In this case-control study a total of 18 endometrial tissues obtained from 10 women with unexplained infertility and 8 normal and fertile women by endometrial biopsy, 6 to 10 days after LH surge. Specimens were fixed in 4% paraformaldhyde fixative and frozen sectioned for semi-quantitative immunohistochemical evaluation using monoclonal anti-human eNOS antibody. Hematoxilin and Eosin was used for Histological dating. Results: Localization of endothelial nitric oxide synthase was seen in glandular and luminal epithelium, vascular endothelium and stroma in both fertile women and women with unexplained infertility. Although there were differences in immunoreactivity of glandular epithelium (p=0.44), vascular endothelium (p=0.60) and stroma (p=0.63) but only over-expression of eNOS in luminal epithelium (p=0.045) of women with unexplained infertility compared to fertile women was statistically significant (p<0.05).
Conclusion: This study suggests that changes in luminal expression of eNOS may influence receptivity of endometrium.
Full-Text [PDF 580 kb]   (58 Downloads) |   |   Full-Text (HTML)  (6 Views)  
Type of Study: Original Article |
Received: 2017/10/1

References
1. Taylor A. ABC of subfertility: extent of the problem. BMJ 2003; 327: 434-436. [DOI:10.1136/bmj.327.7412.434]
2. Smith S, Pfeifer SM, Collins JA. Diagnosis and management of female infertility. JAMA 2003; 290: 1767-1770. [DOI:10.1001/jama.290.13.1767]
3. Griffith OW, Stuehr DJ. Nitric oxide synthase properties and catalytic mechanism. Ann Rev Physiol 1995; 57: 707-736. [DOI:10.1146/annurev.ph.57.030195.003423]
4. Marletta MA. Nitric oxide: biosynthesis and biological significance. Trends Biochem Sci 1989; 14: 488-492. [DOI:10.1016/0968-0004(89)90181-3]
5. Khorram O, Garthwaite M, Magness RR. Endometrial and myometrial expression of nitric oxide synthase isoforms in pre- and postmenopausal women. J Clin Endocrinol Metab 1999; 84: 2226-2232. [DOI:10.1210/jc.84.6.2226]
6. Oztuzcu S, Igci YZ, Arslan A, Sivasli E, Ozkara E, Igci M, Demiryürek S, et al. mRNA expressions of inducible nitric oxide synthase, endothelial nitric oxide synthase, and neuronal nitric oxide synthase genes in meningitis patients. Genet Test Mol Biomarkers 2011; 15: 147-152. [DOI:10.1089/gtmb.2010.0142]
7. Khorram O. The role of nitric oxide in implantation. Rev Endcor Metab Disors 2002; 3: 145-149 [DOI:10.1023/A:1015459029397]
8. World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction, 4th Ed. Cambridge: Cambridge University Press; 1999: 128.
9. Noyes RW, Hertig AT, Rock J. Dating the endometrial biopsy. Fertil Steril 1950; 1: 3-25. [DOI:10.1016/S0015-0282(16)30062-0]
10. Bhuiyan MB, Murad F, Fant ME. The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide. Cell Commun Signal 2006; 10: 4. [DOI:10.1186/1478-811X-4-4]
11. Taguchi M, Alfer J, Chwalisz K, Beier HM, Classen-Linke I. Endothelial nitric oxide synthase is differently expressed in human endometrial vessels during the menstrual cycle. Mol Hum Reprod 2000; 6: 185-190. [DOI:10.1093/molehr/6.2.185]
12. Telfer JF, Irvine GA, Kohnen G, Campbell S, Cameron IT. Expression of endothelial and inducible nitric oxide synthase in non-pregnant and decidualized human endometrium. Mol Hum Reprod 1997; 3: 69-75. [DOI:10.1093/molehr/3.1.69]
13. Karck U, Reister F, Schafer W, Zahradnik HP, Breckwoldt M. PGE2 and PGF2 alpha release by human peritoneal macrophages in endometriosis. Prostaglandins 1996; 51: 49-60. [DOI:10.1016/0090-6980(95)00159-X]
14. Khorram O, Han G. Influence of progesterone on endometrial nitric oxide synthase expression. Fertil Steril 2009; 91 (Suppl.): 2157-2162. [DOI:10.1016/j.fertnstert.2008.05.019]
15. Dong YL, Yallampalli C. Interaction between nitric oxide and prostaglandin E2 pathways in pregnant rat uteri. Am J Physiol 1996; 270: 471-476.
16. Khorram O, Han G, Magee T. Cigarette smoke inhibits endometrial epithelial cell proliferation through a nitric oxide-mediated pathway. Fertil Steril 2010; 93: 257-263. [DOI:10.1016/j.fertnstert.2008.09.074]

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