Volume 16, Issue 3 (March 2018)                   IJRM 2018, 16(3): 199-208 | Back to browse issues page

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Fazaeli H, Davoodi F, Kalhor N, Tabatabaii Qomi R. The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT. IJRM. 2018; 16 (3) :199-208
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1026-en.html
1- Research Department, Highly Specialized Jihad Daneshgahi Infertility Treatment Center, Academic Center of Education, Culture and Research (ACECR), Qom, Iran
2- Research Department, Highly Specialized Jihad Daneshgahi Infertility Treatment Center, Academic Center of Education, Culture and Research (ACECR), Qom, Iran , taba_1359@yahoo.com
Abstract:   (513 Views)
Background: One of the most important involved factors in pregnancy occurrence following intrauterine insemination (IUI) is semen sample preparation. Recently, supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been shown to improve semen parameters.
Objective: To compare the effect of preparation methods in order to IUI, SPAS and density gradient centrifugation (DGC).
Materials and Methods: This trial was done on 80 couples with male factor infertility who attend jihad daneshgahi infertility treatment center of Qom province, undergoing ovarian stimulation and IUI cycle. Various semen parameters including motility, count, DNA fragmentation and capacitation were evaluated before and after preparation. The effect of semen preparation methods and influence of various semen parameters on pregnancy occurrence were examined.
Results: The overall clinical pregnancy rate was 17.5% per patient with no miscarriage. The pregnancy rate for DGC and SPAS were 5% (2 of 40) and 30% (12 of 40) respectively. Since there is no significant difference in improving motion parameters between two groups (except recovery of total number of motile spermatozoa), it seems that these parameters alone are not sufficient to predict IUI pregnancy outcome whereas in samples with >25 million motile spermatozoa in inseminate, there was a clear trend for a higher pregnancy rate for the sample processed using SPAS.
Conclusion: Considering SPAS as a new and effective method leading to provide a combination of various improved semen parameters, is expected in near future.
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Type of Study: Original Article |

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