Volume 15, Issue 11 (11-2017)                   IJRM 2017, 15(11): 713-718 | Back to browse issues page


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Joolayi F, Navidifar T, Mohammad Jaafari R, Amin M. Comparison of Chlamydia trachomatis infection among infertile and fertile women in Ahvaz, Iran: A case-control study. IJRM. 2017; 15 (11) :713-718
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-903-en.html
1- Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Reproductive Health Promotion Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , mnsamin@yahoo.com
Abstract:   (489 Views)
Background: Chlamydia trachomatis (C. trachomatis) is the main cause of bacterial sexually transmitted infections. In women, this infection can lead to tubal infertility. Objective: In this study we investigated C. trachomatis among infertile and fertile women with both polymerase chain reaction (PCR) and ELISA methods in Ahvaz, Iran. Materials and Methods: This case-control study was conducted at the Infertility Clinic of University Jahad, Ahvaz, Iran from January to August 2017. A total of 225 vaginal swabs and blood samples (100 infertile and 125 fertile women) were collected. Detection of C. trachomatis DNA was performed from vaginal swabs by amplification of MOMP gene. Also, anti C. trachomatis immunoglobulin M (IgM) and immunoglobulin G antibodies in the serum samples were recognized by enzyme-linked immunosorbent assay (ELISA). Results: Results showed that, 6 (6%) infertile and 2 (1.6%) fertile women were positive for IgM (p=0.21). Also, PCR was positive for C. trachomatis infection in 5 infertile (5%) and 2 fertile women (1.6%) (p=0.35). We did not find any seropositive immunoglobulin G in both groups. Conclusion: In this study, no significant difference was found between fertile and infertile groups for C. trachomatis infection. Also, the correlation between IgM and PCR results revealed a relatively strong agreement and seems both PCR and IgM assays are appropriate for the accurate diagnosis of C. trachomatis infections.
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Type of Study: Original Article |
Received: 2018/01/8 | Accepted: 2018/01/8 | Published: 2018/01/8

References
1. Weinstock H, Berman S, Cates WJr. Sexually transmitted diseases among American youth: incidence and prevalence estimates, 2000. Perspect Sex Reprod Health 2004; 36: 6-10. [DOI:10.1363/3600604]
2. World Health Organization. Global prevalence and incidence of selected curable sexually transmitted diseases: overview and estimates. WHO/GPA/STD/95. Geneva: WHO, 1995.
3. Miller KE. Diagnosis and treatment of Chlamydia trachomatis infection. Am Fam Physician 2006; 73: 1411-1416.
4. Mardh PA. Tubal factor infertility, with special regard to Chlamydial salpingitis. Curr Opin Infect Dis 2004; 17: 49-52. [DOI:10.1097/00001432-200402000-00010]
5. Svensson LO, Mares I, Olsson SE, Nordström ML. Screening for Chlamydia trachomatis infection in women and aspects of the laboratory diagnostics. Acta Obstet Gynecol Scand 1991; 70: 587-590. [DOI:10.3109/00016349109007921]
6. Novak M, Novak D. Risk factors for Chlamydia trachomatis infection among users of an internet-based testing service in sweden. Sex Reprod Health 2013; 4: 23-27. [DOI:10.1016/j.srhc.2012.11.004]
7. Hamdad-Daoudi F, Petit J, Eb F. Assessment of Chlamydia trachomatis infection in asymptomatic male partners of infertile couples. J Med Microbiol 2004; 53: 985-990. [DOI:10.1099/jmm.0.45641-0]
8. Taylor BD, Haggerty CL. Management of Chlamydia trachomatis genital tract infection: screening and treatment challenges. Infect Drug Resist 2011; 4: 19-29. [DOI:10.2147/IDR.S12715]
9. Malhotra M, Sood S, Mukherjee A, Muralidhar S, Bala M. Genital Chlamydia trachomatis: an update. Indian J Med Res 2013; 138: 303-316.
10. Saison F, Mahilum-Tapay L, Michel CE, Buttress ND, Nadala EC Jr, Magbanua JP, et al. Prevalence of Chlamydia trachomatis infection among low- and high-risk Filipino women and performance of Chlamydia rapid tests in resource-limited settings. J Clin Microbiol 2007; 45: 4011-4017. [DOI:10.1128/JCM.01343-07]
11. Meyer T. Diagnostic procedures to detect Chlamydia trachomatis infections. Microorganisms 2016; 4: pii: E25.
12. Centers for Disease Control and Prevention. Recommendations for the laboratory-based detection of Chlamydia trachomatis and neisseria gonorrhoeae-2014. MMWR Recomm Rep 2014; 63: 1-19.
13. Sattari M, Ghiami Rad M, Ghasemzadeh A, Mohammadoghli Reihan Z. Frequency of anti-Chlamydia trachomatis antibodies in infertile women referred to Tabriz Al-Zahra Hospital. Int J Reprod Biomed 2017; 15: 17-20.
14. Badami N, Salari MH. Rate of Chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum in infertile females and control group. Iran J Public Health 2001; 30: 57-60.
15. Rashidi BH, Chamani-Tabriz L, Haghollahi F, Jeddi-Tehrani M, Naghizadeh MM, Shariat M, et al. Effects of Chlamydia trachomatis infection on fertility; A case-control study. J Reprod Infertil 2013; 14: 67-72.
16. Kalantar SM, Kazemi MJ, Sheikhha MH, Aflatoonian A, Kafilzadeh F. Detection of chlamydia trachomatis infection in female partners of infertile couples. Int J Fertil Steril 2007; 1: 79-84.
17. de Lima Freitas NS, Borborema-Santos CM, Barroso Serrão das Neves D, Costa de Oliveira CM, Dutra Ferreira JR, Astolfi-Filho S. High prevalence detection of Chlamydia trachomatis by polymerase chain reaction in endocervical samples of infertile women attending university hospital in Manaus-Amazonas, Brazil. Gynecol Obstet Invest 2011; 72: 220-226 [DOI:10.1159/000324798]
18. Muvunyi CM, Dhont N, Verhelst R, Temmerman M, Claeys G, Padalko E. Chlamydia trachomatis infection in fertile and subfertile women in Rwanda: prevalence and diagnostic significance of IgG and IgA antibodies testing. Hum Reprod 2011; 26: 3319-3326. [DOI:10.1093/humrep/der350]
19. Al-Ramahi M, Mahafzah A, Saleh S, Fram K. Prevalence of chlamydia trachomatis infection in infertile women at a university Hospital in Jordan. East Mediterr Health J 2008; 14: 1148-1154.
20. Malik A, Jain S, Rizvi M, Shukla I, Hakim S. Chlamydia trachomatis infection in women with secondary infertility. Fertil Steril 2009; 91: 91-95. [DOI:10.1016/j.fertnstert.2007.05.070]
21. Marashi SM, Moulana Z, Imani Fooladi AA, Mashhadi Karim M. Comparison of genital chlamydia trachomatis infection incidence between women with infertility and healthy women in Iran using PCR and immunofluorescence methods. Jundishapur J Microbiol 2014; 7: e9450. [DOI:10.5812/jjm.9450]

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