Volume 15, Issue 9 (9-2017)                   IJRM 2017, 15(9): 569-574 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohammadi M, Farazmandfar T, Shahbazi M. Relationship between human leukocyte antigen (HLA)-DQA1*0102/HLA-DQB1*0602 polymorphism and preeclampsia. IJRM. 2017; 15 (9) :569-574
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-860-en.html
1- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
2- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran. , shahbazimajid@yahoo.co.uk
Abstract:   (926 Views)
Background: Preeclampsia is a condition associated with systemic disorders in the mother and the fetus. However, the exact causes of preeclampsia are unknown, but several genetics and environmental factors play role in development of this disease. Major histocompatibility complex role is very important during pregnancy through which the fetus is not rejected by mother’s immune system.
Objective: In this study, we investigated the relationship of the human leukocyte antigen (HLA)-DQA1*0102/HLA-DQB1*0602 polymorphism with preeclampsia.
Materials and Methods: Genomic DNA of 181 pregnant women with a history of preeclampsia as the case group and 228 pregnant women with no history of preeclampsia as the controls were extracted. The HLA-DQA1*0102/HLA-DQB1*0602 polymorphisms of all DNA samples were identified by the SSP-PCR method. Frequencies difference of variables between case and control groups were calculated by Chi-square test. The ethnic origin of the participants in this study was extracted from their medical records.
Results: There was a significant association between preeclampsia and Sistani ethnic group (p=0.031). Moreover, there was a significant association between preeclampsia and frequencies of allele HLA-DQB1*0602 (p<0.001), and genotypes of heterozygote (+0102/-0602) (p<0.001) and negative homozygote (-0102/-0602) (p=0.005). There also was an association between allele HLA-DQB1*0602 and preeclampsia in Fars ethnic group (p=0.028).
Conclusion: It seems that immune incompatibility may have an important role in preeclampsia predisposition. According to our results, the lack of locus HLA-DQB1*0602 may be a risk factor for preeclampsia.
Full-Text [PDF 383 kb]   (340 Downloads) |   |   Full-Text (HTML)  (97 Views)  
Type of Study: Original Article |

1. Hauth JC, Ewell MG, Levine RJ, Esterlitz JR, Sibai B, Curet LB, et al. Pregnancy outcomes in healthy nulliparas who developed hypertension. Calcium for Preeclampsia Prevention Study Group. Obstet Gynecol 2000; 95: 24-28.
2. Hnat MD, Mercer BM, Thurnau G, Goldenberg R, Thom EA, Meis PJ, et al. Perinatal outcomes in women with preterm rupture of membranes between 24 and 32 weeks of gestation and a history of vaginal bleeding. Am J Obstet Gynecol 2005; 193: 164-168. [DOI:10.1016/j.ajog.2004.10.625]
3. Eiland E, Nzerue C, Faulkner M. Preeclampsia 2012. J Pregnancy 2012; 2012: 586578. [DOI:10.1155/2012/586578]
4. Mutter WP, Karumanchi SA. Molecular mechanisms of preeclampsia. Microvasc Res 2008; 75: 1-8. [DOI:10.1016/j.mvr.2007.04.009]
5. Chaouat G, Ledee-bataille N, Zourbas S, Dubanchet S, Sandra O, Martal J, et al. Implantation: can immunological parameters of implantation failure be of interest for pre-eclampsia? J Reprod Immunol 2003; 59: 205-217. [DOI:10.1016/S0165-0378(03)00048-2]
6. Hung TH, Burton GJ. Hypoxia and reoxygenation: a possible mechanism for placental oxidative stress in preeclampsia. Taiwan J Obstet Gynecol 2006; 45: 189-200. [DOI:10.1016/S1028-4559(09)60224-2]
7. Bernard N, Giguère Y. Genetics of preeclampsia: what are the challenges? J Obstet Gynaecol Can 2003; 25: 578-585. [DOI:10.1016/S1701-2163(16)31017-9]
8. Redman CW, Sargent IL. Latest advances in understanding preeclampsia. Science 2005; 308: 1592-1594. [DOI:10.1126/science.1111726]
9. Moffett A, Hiby SE, Sharkey AM. The role of the maternal immune system in the regulation of human birthweight. Philos Trans R Soc Lond B Biol Sci 2015; 370: 20140071. [DOI:10.1098/rstb.2014.0071]
10. Martínez-Borra J, López-Larrea C. The emergence of the major histocompatilibility complex. Adv Exp Med Biol 2012; 738: 277-289. [DOI:10.1007/978-1-4614-1680-7_16]
11. Hviid TVF. HLA-G in human reproduction: aspects of genetics, function and pregnancy complications. Hum Reprod Update 2006; 12: 209-232. [DOI:10.1093/humupd/dmi048]
12. Klitz W, Maiers M, Spellman S, Baxter-Lowe LA, Schmeckpeper B, Williams TM, et al. New HLA haplotype frequency reference standards: high-resolution and large sample typing of HLA DR-DQ haplotypes in a sample of European Americans. Tissue Antigens 2003; 62: 296-307. [DOI:10.1034/j.1399-0039.2003.00103.x]
13. Zamani M, Mehri M, Kollaee A, Yenki P, Ghaffarpor M, Harirchian MH, et al. Pharmacogenetic Study on the Effect of Rivastigmine on PS2 and APOE Genes in Iranian Alzheimer Patients. Dement Geriatr Cogn Dis Extra 2011; 1: 180-189. [DOI:10.1159/000329514]
14. Farazmandfar T, Haghshenas MR, Janbabai G, Azadeh H, Sharifian R, Taghipour M. A rapid and reliable genotyping method for hepatitis B virus genotypes (A-H) using type-specific primers. J Virol Methods 2012; 181: 114-116. [DOI:10.1016/j.jviromet.2012.01.025]
15. Laresgoiti-Servitje E, Gómez-López N, Olson DM. An immunological insight into the origins of pre-eclampsia. Hum Reprod Update 2010; 16: 510-524. [DOI:10.1093/humupd/dmq007]
16. Han L, Liu X, Li H, Zou J, Yang Z, Han J, et al. Blood coagulation parameters and platelet indices: changes in normal and preeclamptic pregnancies and predictive values for preeclampsia. PloS One 2014; 9: e114488. [DOI:10.1371/journal.pone.0114488]
17. Humphrey KE, Harrison GA, Cooper DW, Wilton AN, Brennecke SP, Trudinger BJ. HLA-G deletion polymorphism and pre-eclampsia/eclampsia. Br J Obstet Gynaecol 1995; 102: 707-710. [DOI:10.1111/j.1471-0528.1995.tb11427.x]
18. Hunt JS, Langat DK, McIntire RH, Morales PJ. The role of HLA-G in human pregnancy. Reprod Biol Endocrinol 2006; 4: 1-8. [DOI:10.1186/1477-7827-4-S1-S10]
19. Pfeiffer KA, Fimmers R, Engels G, van der Ven H, van der Ven K. The HLA-G genotype is potentially associated with idiopathic recurrent spontaneous abortion. Mol Hum Reprod 2001; 7: 373-378. [DOI:10.1093/molehr/7.4.373]
20. Aldrich CL, Stephenson MD, Karrison T, Odem RR, Branch DW, Scott JR, et al. HLA-G genotypes and pregnancy outcome in couples with unexplained recurrent miscarriage. Mol Hum Reprod 2001; 7: 1167-1172. [DOI:10.1093/molehr/7.12.1167]
21. Mao Y, Zhang Z, Fan L, Wu Q, Jiang L, Yang J, et al. HLA-DQA1, -DQB1 polymorphism distribution in Chinese women with pregnancy induced hypertension in Shanghai area. Chin Med J (Engl) 1998; 111: 163-165.
22. Honda K, Takakuwa K, Hataya I, Yasuda M, Kurabayashi T, Tanaka K. HLA-DQB1 and HLA-DPB1 genotypes in severe preeclampsia. Obstet Gynecol 2000; 96: 385-389.
23. Ooki I, Takakuwa K, Akashi M, Nonaka T, Yokoo T, Tanaka K. Studies on the compatibility of HLA-Class II alleles in patient couples with severe pre-eclampsia using PCR-RFLP methods. Am J Reprod Immunol 2008; 60: 75-84. [DOI:10.1111/j.1600-0897.2008.00592.x]

Send email to the article author

© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb