Volume 5, Issue 5 (7-2007)                   IJRM 2007, 5(5): 171-176 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Talebi A R, Khalili M A, Nahangi H, Abbasi A, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat. IJRM. 2007; 5 (5) :171-176
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-85-en.html
1- Research and Clinical Center for Infertility, and Department of Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, and Department of Anatomy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , khalili59@hotmail.com
Abstract:   (542 Views)
Background: Spinal cord injury (SCI) occurs most often to young men at the peak of their reproductive health. Only 10% of SCI men can father children without medical assistance due to potential impairments in ejaculation and sperm quality.
Objective: The main objective of this experimental study was to evaluate the epididymal necrospermia- sperm death, after chronic SCI in rat.
Materials and Methods: Forty-five adult Wistar rats were divided into 3 groups of SCI, sham, and control. Following laminectomy, SCI was induced onto exposed dura matter (T10) of anesthetized rats. Sham group underwent laminectomy of T10 only; while, control rats were not exposed to any type of injury or medication. The spermatozoa from cauda epididymis were aspirated after 50 days for analysis of necrospermia with three assays of Eosin-Y staining, Hypo-osmotic swelling (HOS), and Hoechst 33258 fluorescent dye.
Results: The rate of necrospermia in SCI rats was significantly increased when compared with other groups (p<0.05). Also, the rates of necrspermia in SCI samples were similar with application of 3 assays (Eosin-Y: 46.11±9.41; HOS: 45.88±8.89; Hoechst: 46.76±9.31). Total necrospermia was not observed in any of the epididymal samples.
Conclusion: The results showed that chronic SCI is associated with high rate of epididymal necrospermia in mammals such as rats. It is, therefore, recommended that an effective laboratory technique, such as Hoechst 33258 should be used for separation of live and motile sperms from necrospermic ones for assisted reproduction program.
Full-Text [PDF 70 kb]   (150 Downloads) |   |   Full-Text (HTML)  (25 Views)  
Type of Study: Original Article |

References
1. Kafetsoulis A, Brackett NL, Ibrahim E, Attia GR, Lynne CM. Current trends in the treatment of infertility in men with spinal cord injury. Fertil Steril 2006; 86: 781-789. [DOI:10.1016/j.fertnstert.2006.01.060]
2. Rajasekaran M, Monga M. Cellular and molecular causes of male infertility in spinal cord injury. J Androl 1999; 20:326-330.
3. Brackett NL, Bloch WE, Lynne CM. Predictors of necrospermia in men with spinal cord injury. J Urol 1998; 159: 844-847. [DOI:10.1016/S0022-5347(01)63749-2]
4. World Health Organization WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. N.Y. Cambridge University Press.1999
5. Mallidis C, Lim T, Hill ST, Skinner DJ, Brown DJ, Johnson IH, Baker HWG. Necrospermia and spinal cord injury. Fertil Steril 2000; 74: 221-227. [DOI:10.1016/S0015-0282(00)00650-6]
6. Wilton LJ, Temple-Smith PD, Baker HWG. Human male infertility caused by degeneration and death of sperm in the epididymis. Fertil Steril 1988; 49:1052-1058. [DOI:10.1016/S0015-0282(16)59960-9]
7. Hirsch LH, Huang B, Chancellor MB, Rivas DA, Salzman SK, Jost LK, et al. Spermatogenesis in early and chronic phases of experimental spinal cord injury in the rodent. J Androl 1999; 20:63-71.
8. Aitken RJ, Buckingham D, Harkiss D. Use of a xanthine oxidase free radical generating system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa. J. Reprod Fertil 1993; 97:441-450. [DOI:10.1530/jrf.0.0970441]
9. Khalili MA, Mir-Rokni F., Kalantar SM. Application of vitality tests on asthenozoospermic semen from infertile men. Iran Biomed. J 1999; 3:77-81.
10. Esteves SC, Sharma RK, Thomas AJ, Agarwal A. Cryopreservation ofhuman spermatozoa with pentoxifylline improves the post-thaw agonist-induced acrosome reaction rate. Hum Reprod 1998; 13:3384-3389. [DOI:10.1093/humrep/13.12.3384]
11. Cross NL, morales P, Overstreet JW, Hanson FW Two simple methods for detecting acrosome-reacted human sperm. Gamete Res 1986; 15:213-226. [DOI:10.1002/mrd.1120150303]
12. Hinsch E, Ponce AA, Hagele W, Hedrich F, Muller-Schlosser F, Schill WB, et al A new combined in-vitro test model for the identification of substances affecting essential sperm functions. Hum Reprod 1997; 12:1673-1681. [DOI:10.1093/humrep/12.8.1673]
13. Kempinas WDC, Suarez JD, Roberts SN, Strader LF, Ferrell J, Goldman JM, et al. Fertility of rat epididymal sperm after chemically and surgically induced sympathectomy. Bio. Reprod 1998; 59:897-904. [DOI:10.1095/biolreprod59.4.897]
14. Brown DJ, Hill ST, Baker HW. Male fertility and sexual function after spinal cord injury. Progressive Brain Res 2006; 152:427-439. [DOI:10.1016/S0079-6123(05)52029-6]
15. Huang HF, Li MT. Spinal cord contusion impairs sperm motility in the rat without disrupting spermatogenesis J. Androl 2003; 24:371-380. [DOI:10.1002/j.1939-4640.2003.tb02685.x]
16. Linsenmeyer TA, Ottenweller JE, Huang HFS. Epididymal sperm transport in normal and recent spinal cord injured Sprague Dawley rats. J. Spinal Cord Med 1999; 22:102-106. [DOI:10.1080/10790268.1999.11719554]
17. Talebi AR, Khalili MA, Hussaini A. Assessment of DNA integrity of epididymal spermatozoa following experimental chronic spinal injury in rat. Int J Androl 2007; 30:163-169. [DOI:10.1111/j.1365-2605.2006.00736.x]
18. Wilton LJ, Temple-Smith PD, Baker HWG, de Kretser DM. Human male infertility caused by degeneration and death of sperm in the epididymis Fertil Steril 1998; 49: 1052-1058. [DOI:10.1016/S0015-0282(16)59960-9]
19. Padron OF, Brackett NL, Sharma RK, Lynne CM, Thomas AJ, Agarwal A. Seminal reactive oxygen species and sperm motility and morphology in men with spinal cord injury. Fertil Steril 1997; 67:1115-1119. [DOI:10.1016/S0015-0282(97)81448-3]
20. DeForge D, Blackmer J, Garritty C, Yazdi F, Cronin V, Barrowman N, et al. Fertility following spinal cord injury: a systematic review. Spinal Cord 2005; 43:693-703. [DOI:10.1038/sj.sc.3101769]
21. Utida C, Truzzi JC, Bruschini H, Simonetti R, Cedenho AP, Srougi M, et al. Male infertility in spinal cord trauma. Int. Brazil. J Urol 2005; 31:375-383. [DOI:10.1590/S1677-55382005000400013]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb