Volume 15, Issue 8 (9-2017)                   IJRM 2017, 15(8): 509-520 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Arabameri A, Sameni H, Bandegi A. The effects of propolis extract on ovarian tissue and oxidative stress in rats with maternal separation stress. IJRM. 2017; 15 (8) :509-520
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-847-en.html
1- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Damghan, Iran
2- Nervous System Stem Cells Research Center, Department of Anatomical Sciences, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran , hrsameni@gmail.com
3- Nervous System Stem Cells Research Center, Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
Abstract:   (885 Views)
Background: Stress in infancy has dramatic effects on different systems, including the nervous system, endocrine, immune, reproductive and etc.
Objective: The purpose of this study was to investigate the effects of extract of Iranian propolis (EIP) on ovarian tissue and oxidative stress in rats with maternal separation stress.
Materials and Methods: 48 immature female rats were divided randomly into six groups. 1) Control group, 2) Control group+saline, 3) Stress group, includes infants that were separated from their mothers 6 hr/day, the 4th, 5th and 6th groups consisted of infants who in addition to daily stress received 50, 100 and 200 mg/kg of EIP, respectively. Then serum corticosterone, 17-beta-estradiol, malondialdehyde, total superoxide dismutase, glutathione peroxidase and ferric reducing antioxidant power levels were measured. The ovarian sections were stained by H&E, PAS, and TUNEL methods and were studied with optical microscopy.
Results: Stress increased the blood serum corticosterone levels and 17-beta-estradiol reduced significantly (p<0.001) and EIP prevented from this changes (p<0.01). EIP significantly increased the number of ovarian follicles, oocytes and oocytes diameter in neonatal rat following stress (p<0.01). EIP also significantly decreased the number of atretic follicles, TUNEL+granulosa cells, malondialdehyde levels and increased ferric reducing antioxidant power, total superoxide dismutase and glutathione peroxidase serum levels in neonatal rats following stress. The dose of 200 mg/kg EIP was more effective.
Conclusion: This Study showed that the Iranian Propolis significantly could prevent oxidative stress and histopathological changes in the ovary of the neonatal rat the following stress.
Full-Text [PDF 953 kb]   (272 Downloads) |   |   Full-Text (HTML)  (76 Views)  
Type of Study: Original Article |

References
1. DiPietro JA, Hilton SC, Hawkins M, Costigan KA, Pressman EK. Influence of maternal stress on fetal behavior and brain development. Biol Neonate 2002; 79: 168-171.
2. Charil A, P.Laplante D, Vaillancourt C, King S. Prenatal stress and brain development. Brain Res Rev 2010; 65: 56-79. [DOI:10.1016/j.brainresrev.2010.06.002]
3. Tilbrook A J, Turner A I, Clarke IJ. Effects of stress on reproduction in non-rodent mammals: the role of glucocorticoids and sex differences. Rev Reprod 2000; 5: 105-113. [DOI:10.1530/ror.0.0050105]
4. Bhat Manjula S, Yajurvedi HN. Stress induced alterations in pre-pubertal ovarian follicular development in rat. J Stress Physiol Biochem 2011; 4: 51-68.
5. Ochiai T, Ohno S, Soeda S, Tanaka H, Shoyama Y, Shimeno H. Crocine prevent the death of rat pheochromyctoma (pc12) cells by its antioxidant effects stronger than those of alphatocopherol. Neurosci Let 2004; 362: 61-64. [DOI:10.1016/j.neulet.2004.02.067]
6. Toufexis D, Angelica Rivarola M, Lara H, Viau V. Stress and the reproductive axis. J Neuroendocrinol 2014; 26: 573-586. [DOI:10.1111/jne.12179]
7. Wolf O. HPA axis and memory, best practice and researche. Endocrinol Metab Clin 2003; 17: 287-299.
8. McGaugh J, Roozendaal B. Role of adrenal stress hormones in forming lasting memories in the brain. Curr Opin Neurobiol 2002; 12: 205-210. [DOI:10.1016/S0959-4388(02)00306-9]
9. Chatterjee A, Chatterjee R. How stress affects female reproduction: An overview. Biomed Res 2009; 20: 79-83.
10. Sato H, Takeyuki T, Sumitani K, Tkastu H, Urano S. Glucocorticoid Generates ROS to Induce Oxidative Injury in the Hippocampus, Leading to Impairment of Cognitive Function of Rats. J Clin Biochem Nutr 2010; 47: 224-232. [DOI:10.3164/jcbn.10-58]
11. Seven PT, Yilmaz S, Sesdszven I, Kelestemur GT. The Effects of Propolis in Animals Exposed Oxidative Stress: Oxidative Stress- Environmental Induction and Dietary Antioxidants. LushchakVI, InTech 2012; 267-288.
12. Lotfy M. Biological Activity of Bee Propolis in Health and Disease. Asian Pacific J Cancer Prevent 2006; 7: 22-31.
13. Arul Selvan K, Prabhu T. Extraction of Propolis from beehives and characterization of its constituents and medicinal properties. Int J Adv Engineer Technol 2010; 11: 50-53.
14. Safari M, Sameni HR, Badban L, Bandegi AR, Fafaei AA, Rashidypour A, et al. Protective effects of water extract of Propolis on dopaminergic Neuronsneurons, brain derived neurotrophic factor, and stress oxidative factors in the rat model of Parkinson's disease. Int J Pharmacol 2015; 11: 300-308. [DOI:10.3923/ijp.2015.300.308]
15. Ghanbari E, Nejati V, Khazaei M. Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats. Int J Reprod Biomed 2016; 14: 519-526.
16. Sameni HR, Ramhormozi P, Bandegim AR, Taherian AB, Mirmohammadkhani M, Safari M. Effects of ethanol extract of propolis on histopathological changes and anti-oxidant defense of kidney in a rat model for type 1 diabetes mellitus. J Diabetes Investig 2016; 7: 506-513. [DOI:10.1111/jdi.12459]
17. Sameni HR, Kavakebian F, Amjad MH, Bandegi AR,Yousefi B, Taherian AA. Effects of hydroalcoholic extract of Propolis on oxidative stress indices of rat fetal brain induced by chronic prenatal stress. Koomesh 2014; 15: 482-492.
18. Sameni HR, Ramhormozi P, Bandegi AR, Taherian AA, Safari M, Amjad MH. Effects of hydroalcoholic extract of Iranian Propolis on blood serum biochemical factors in streptozotocin-induced diabetic rats. Koomesh 2014; 15: 388-395.
19. Bogdanov S. Functional and biological properties of the bee products. Bee Product Sci 2011; 1-12. www.bee-hexagon.net.
20. Bhat Manjula S, Yajurvedi HN. Effects of neonatal stress on ovarian follicular reserve and initial follicular waves in rats. J Adv Lab Res Biol 2011; 2: 175-184.
21. Abdullah KH. Effect of stress hormone antagonists on ovarian follicular development in pre-pubertal rat. J Stress Physiol Biochem 2012; 8: 82-98.
22. Ghassemi L, Zabihi M, Mahdavi R, Seyedmajidi M, Akram S, Motallebnejad M. The effect of ethanolic extract of propolis on radiation-induced mucositis in rats. Saudi Med J 2010; 31: 622-626.
23. Greenaway W, Scaysbrook T, Whatley FR. The composition and plant origins of propolis: a report of work at Oxford. Bee World 1990; 71: 107-118. [DOI:10.1080/0005772X.1990.11099047]
24. Kim JG, Jung HS, Kim KJ, Min SS and Yoon BJ. Basal blood corticosterone level is correlated with susceptibility to chronic restraint stress in mice. Neurosci Lett 2013; 555: 137-142. [DOI:10.1016/j.neulet.2013.09.031]
25. Boutabet K, Kebsa W, Alyane M, Lahouel M. Polyphenolic fraction of Algerian propolis protects rat kidney against acute oxidative stress induced by doxorubicin. Pharm Indian J Nephrol 2011; 21: 101-106. [DOI:10.4103/0971-4065.82131]
26. Montilla P, Munoz M, Castaneda I, Tunez I. Red wine prevents brain oxidative stress and nephropathy in streptozotocin-induced diabetic rats. J Biochem Mol Biol 2005; 38: 539-544. [DOI:10.5483/BMBRep.2005.38.5.539]
27. Pedersen T, Peters H. Proposal for a classification of oocytes and follicles in the mouse ovary. J Reprod Fert 1968; 17: 555-557. [DOI:10.1530/jrf.0.0170555]
28. Benzie IF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of ''Antioxidant Power'': The FRAP Assay. Anal Biochem 1996; 239: 70-76. [DOI:10.1006/abio.1996.0292]
29. Peters H. The Development of the mouse ovary from birth to maturity. Acta Endocrinol 1969; 62: 98-116. [DOI:10.1530/acta.0.0620098]
30. Lunenfeld B, Insler V. Follicular development and its control. In the physiology of reproduction. Gynecol Endocrinol 1993;7:285-291. [DOI:10.3109/09513599309152514]
31. Carr JA, Norris DO. The adrenal gland. In: Endocrine disruption. Biological basis for health effects in wild life and humans. Oxford university press, USA; 2005: 111-134.
32. Guigon CJ, Mazaud S, Forest MG, Brailly Tabard S, Coudouel N, Marge S. Unaltered development of the initial follicular waves and normal pubertal onset in female rats after neonatal deletion of the follicular waves. Endocrinology 2003; 144: 3651- 3662. [DOI:10.1210/en.2003-0072]
33. Okamoto Y, Tobe T, Ueda K, Takada T, Kojima N. Oral administration of Brazilian propolis exerts estrogenic effect in ovariectomized rats. J Toxicol Sci 2015; 40: 235-242. [DOI:10.2131/jts.40.235]
34. Vaya J, Aviram M. Nutritional antioxidants: mechanism of action, analyses of activities and medical applications. Endocr Metab Agents 2001; 1: 99-117. [DOI:10.2174/1568013013359168]
35. Noorafshan A, Esmail-Zadeh B, Bahmanpour S, Poost-Pasand A. Early stereological changes in liver of Sprague-Dawley rats after streptozotocin injection. Indian J Gastroenterol 2005; 24: 104-107.
36. Halliwell B, Gutteridge JM. Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet 1994; 1: 1396-1397.
37. McGee EA, Hseuh JWA. Initial and cyclic recruitment of ovarian follicles. Endocrin Rev 2000; 21: 200-214. [DOI:10.1210/er.21.2.200]
38. Guney M, Demirin H, Oral B, Ozguner M, Bayhan G, Altuntas I. Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C. Hum ExpToxicol 2007; 26: 491-498. [DOI:10.1177/0960327106077505]
39. Abdullah KH. Effect of stress hormone antagonists on ovarian follicular development in pre-pubertal rat. J Stress Physiol Biochem 2012; 8: 82-98.
40. Devine PJ, Perreault SD, Luderer. Roles of Reactive Oxygen Species and Antioxidants in Ovarian Toxicity. Biol Reprod 2012; 86: 1-10. [DOI:10.1095/biolreprod.111.095224]
41. Chatterjee A, Chatterjee R. How stress affects female reproduction: An overview. Biomed Res 2009; 20: 79-83.
42. Hansen PJ. Effects of heat stress on mammalian reproduction. Phil Trans R Soc B 2009; 364: 3341-3350. [DOI:10.1098/rstb.2009.0131]
43. Soto P and Smith LC. BH4 peptide derived from BclxL and Bax-inhibitor peptide suppresses apoptotic mitochondrial changes in heat stressed bovine oocytes. Mol Reprod Dev 2009; 76: 637-646. [DOI:10.1002/mrd.20986]
44. Jasprica I, Bojic M, Mornar A, Besic E, Bucan K, Medic-Saric M. Evaluation of antioxidative activity of Croatian Propolis samples using DPPH·and ABTS·+ stable free radical assays. Molecules 2007; 12: 1006-1021. [DOI:10.3390/12051006]
45. Mohammadzadeh S, Sharriatpanahi M, Hamedi M, Amanzadeh Y, Sadat Ebrahimi S, Ostad S. Antioxidant power of Iranian propolis extract. Food Chem 2007; 103: 729-733. [DOI:10.1016/j.foodchem.2006.09.014]
46. Fonseca Y, Oliveira F, Vicentini F, Furtado N, Sousa J, Valim Y. Evaluation of the potential of Brazilian Propolis against UV-Induced oxidative stress. Evidence-Based Complement Alternat Med 2010; 2011: 1-8. [DOI:10.1155/2011/863917]
47. Miguel M, Nunes S, Dandlen S, Cavaco A, Antunes M. Phenols and antioxidant activity of hydro-alcoholic extracts of propolis from Algarve, South of Portugal. Food Chem Toxicol 2010; [DOI:10.1016/j.fct.2010.09.014]
48. 48: 3418-3423. 48. Seven I, Aksu T, Seven P. The effects of Propolis on biochemical parameters and activity of antioxidant enzymes in broilers exposed to Lead induced oxidative stress. Asian-Aust J Anim Sci 2010; 23: 1482-1489. [DOI:10.5713/ajas.2010.10009]
49. El-Masry T, Emara A, El-Shitany N. Possible protective effect of propolis against lead induced neurotoxicity in animal model. J Evolution Biol Res 2011; 3: 4-11.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb