Volume 15, Issue 8 (9-2017)                   IJRM 2017, 15(8): 503-508 | Back to browse issues page

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Moshtaghi A, Vaziri H, Sariri R, Shaigan H. Polymorphism of MnSOD (Val16Ala) gene in pregnancies with blighted ovum: A case-control study. IJRM. 2017; 15 (8) :503-508
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-846-en.html
1- Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
2- Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran
3- Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran , sariri@guilan.ac.ir
4- Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (1903 Views)
Background: Blighted ovum is one of the most common reasons for abortion during the first three months of pregnancy. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme in the human immune system. The gene is located on 6q25 chromosome and acts on mitochondrial matrix. In the case of mutation or inactivity of this enzyme, mitochondrial and nuclear DNA will severely be destructed. The most common polymorphism of its gene is Val16Ala.
Objective: The aim was to investigate a possible mutation in pregnant women who had abortion during the first trimester of pregnancy due to blighted ovum.
Materials and Methods: In this case-control study, 34 women were entered as the case and control groups, respectively. Genome DNA was extracted from saliva samples and its genotype was determined using Tetra-primer amplification refractory mutation system polymerase chain reaction technique.
Results: In the case group, 16 (48%) cases had Val/Val genotype, 17 (50%) were heterozygote and had Val/Ala genotype, and 1 (2%) had Ala/Ala genotype. Among controls, 7 (22%) items had Val/Val genotype, 6 (17%) had Val/Ala genotype, and 21 (61%) had Ala/Ala genotype. The frequency of TT, CT, and CC genotypes was 48%, 50%, and 2% in case group and 22%, 17%, and 61% in control group, respectively. Statistical analysis revealed a significant relationship between Val16Ala polymorphism of MnSOD gene and blighted ovum (p= 0.0003).
Conclusion: It has concluded that a significant relationship exists between Val16Ala polymorphism of MnSOD gene and blighted ovum.
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1. Coughlin LB, Roberts D, Haddad NG, Long A. Medical management of first trimester miscarriage (blighted ovum and missed abortion): is it effective? J Obstet Gynaecol 2004; 24: 69-71. [DOI:10.1080/01443610310001620332]
2. Regan L, Rai R. Epidemiology and the medical causes of miscarriage. Baillieres Best Pract Res Clin Obstet Gynaecol. J IJMCM 2000; 14: 839-854.
3. Wang X, Chen C, Wang L, Chen D, Guang W, French J. Conception, early pregnancy loss, and time to clinical pregnancy: a population-based prospective study. Fertil Steril 2003; 79: 577-584. [DOI:10.1016/S0015-0282(02)04694-0]
4. Wyatt PR, Owolabi T, Meier C, Huang T. Age-specific risk of fetal loss observed in a second trimester serum screening population. Am J Obstet Gynecol 2005; 192: 240-246. [DOI:10.1016/j.ajog.2004.06.099]
5. Faghihzadeh S, Babaee Rochee G, Lmyian M, Mansourian F, Rezasoltani P. Factors associated with unwanted pregnancy. J Sex Marital Ther 2003; 29: 157-164. [DOI:10.1080/713847165]
6. Osborn JF, Cattaruzza MS, Spinelli A. Risk of spontaneous abortion in Italy, 1978-1995, and the effect of maternal age, gravidity, marital status, and education. Am J Epidemiol 2000; 151: 98-105. [DOI:10.1093/oxfordjournals.aje.a010128]
7. Shekoohi S, Mojarrad M, Raoofian R, Ahmadzadeh Sh, Mirzaie S, Hassanzadeh-Nazarabadi M. Chromosomal study of couples with the history of recurrent spontaneous abortions with diagnosed blighted ovum. Int J Mol Cell Med 2013; 2: 164-168.
8. Weiberg R. Recurrent Pregnancy loss. In: Speroff L FM (ed). Clinical Gynecologic Endocrinology And Infertitity. Courier Westford Lippincott Williams & Wilkins Inc; 2005: 1070-93.
9. Helgstrand S, Andersen AM. Maternal underweight and the risk of spontaneous abortion. Acta Obstet Gynecol Scand 2005; 84: 1197-1201. [DOI:10.1111/j.0001-6349.2005.00706.x]
10. Munoz M, Arigita M, Bennasar M, Soler A, Sanchez A, Borrel A. Chromosomal anomaly spectrum in early pregnancy loss in relation to presence or absence of an embryonic pole. Fertil Steril 2010; 94: 2564-2568. [DOI:10.1016/j.fertnstert.2010.04.011]
11. von SC, Schuchmann HP. Radical-mediated DNA damage in presence of oxygen. Methods Enzymol 1990; 186: 511-520. [DOI:10.1016/0076-6879(90)86145-L]
12. Lin P, Hsueh YM, Ko JL, Liang YF, Tsai KJ, Chen CY. Analysis of NQO1, GSTP1, and MnSOD genetic polymorphisms on lung cancer risk in Taiwan. Lung Cancer 2003; 40: 123-129. [DOI:10.1016/S0169-5002(03)00027-8]
13. Oberley TD, Oberley LW. Antioxidant enzyme levels in cancer. Histol Histopathol 1997; 12: 525-535.
14. Honda Y, Honda S. The daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabiditis elegans. FASEB J 1999; 13: 1385-1393. [DOI:10.1096/fasebj.13.11.1385]
15. Midorikawa K, Kawanishi S. Superoxide dismutases enhance H2O2- induced DNA damage and alter its site specificity. FEBS Lett 2001; 27: 187-190. [DOI:10.1016/S0014-5793(01)02383-3]
16. Mitrunen K, Sillanpaa P, Katja V, Eskelinen M, Kosma VM, Behamou S, et al. Association between manganese superoxide dismutase (MnSOD) gene polymorphism and breast cancer risk. Carcinogenesis 2001; 22: 827-829. [DOI:10.1093/carcin/22.5.827]
17. Ambrosone CB, Freudenheim JL, Thompson PA, Bowman E, Vena JE, Marshal JR, et al. Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res 1999; 59: 602-606.
18. Albano E. Alcohol. Oxidative stress and free radical damage. Proc Nutr Soc 2006; 65: 278-290. [DOI:10.1079/PNS2006496]
19. Zelko IN, Mariani TJ, Folz RJ. Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Rad Biol Med 2002; 33: 337-349. [DOI:10.1016/S0891-5849(02)00905-X]
20. Bernard KG, Cooperberg PL. Sonographic differentiation between blighted ovum and early viable pregnancy. AJR Am J Roentgenol 1985; 144: 597-602. [DOI:10.2214/ajr.144.3.597]
21. Collins AR, Ma AG, Duthie SJ. The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutat Res 1995; 336: 69-77. [DOI:10.1016/0921-8777(94)00043-6]
22. Van Remmen H, Ikeno Y, Hamilton M, et al. Life-long reduction in MnSOD activity results in increased DNA damage and higher incidence of cancer but does not accelerate aging. Physiol Genomics 2003; 16: 29-37. [DOI:10.1152/physiolgenomics.00122.2003]
23. Parsons PA. Evolutionary adaptation and stress: the fitness gradient. Evol Biol 1992; 26: 191-223. [DOI:10.1007/978-1-4615-3336-8_5]
24. Fu-Tsai Kung. Hysteroscopic differences in the gestational sac in asymptomatic blighted ovum and viable pregnancy at early gestation. Taiwan J Obstet Gynecol 2005; 44: 342-346. [DOI:10.1016/S1028-4559(09)60168-6]
25. Sofia Dória S, Carvalho F, Ramalho C, Lima V, Francisco T, Machado AP, et al. An efficient protocol for the detection of chromosomal abnormalities in spontaneous miscarriages or foetal deaths. Eur J Obstet Gynecol Reprod Biol 2009; 147: 144-150. [DOI:10.1016/j.ejogrb.2009.07.023]
26. van den Berg MMJ, van Maarle MC, van Wely M, Goddijn M. Genetics of early miscarriage. Biochimica et Biophysica Acta 2012; 1822: 1951-1959. [DOI:10.1016/j.bbadis.2012.07.001]
27. Trabetti E, Galavotti R, Zanini L, Zardini N, Zatti F, Bermadi A, et al. The parental origin of hydatidiform moles and blighted ova: molecular probing with hypervariable DNA polymorphisms). Mol Cell Probes 1993; 7: 325-329. [DOI:10.1006/mcpr.1993.1046]
28. Azmanov DN, Milachich TV, Michailova GI, Dimitrova VG, Karagiozova ZH, Maznejkova VT. Profile of chromosomal aberrations in different gestational age spontaneous abortions detected by comparative genomic hybridization. Eur J Obstet Gynecol Reprod Biol 2007; 131: 127-131. [DOI:10.1016/j.ejogrb.2006.04.037]

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