Volume 15, Issue 6 (7-2017)                   IJRM 2017, 15(6): 331-344 | Back to browse issues page


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Pourmasumi S, Sabeti P, Rahiminia T, Mangoli E, Tabibnejad N, Talebi A R. The etiologies of sperm DNA abnormalities in male infertility: An assessment and review. IJRM. 2017; 15 (6) :331-344
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-834-en.html
1- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (2333 Views)
The sperm DNA damage may occur in testis, genital ducts, and also after ejaculation. Mechanisms altering chromatin remodeling are abortive apoptosis and oxidative stress resulting from reactive oxygen species. Three classifications of intratesticular, post-testicular, and external factors have been correlated with increased levels of sperm DNA damage which can affect the potential of fertility. Alcohol consumption may not increase the rate of sperm residual histones and protamine deficiency; however, it causes an increase in the percentage of spermatozoa with DNA fragmentation and apoptosis. In a medical problem as spinal cord injury, poor semen parameters and sperm DNA damage were reported. Infection induces reactive oxygen species production, decreases the total antioxidant capacity and sperm DNA fragmentation or antigen production that lead to sperm dysfunctions and DNA fragmentation. While reactive oxygen species generation increases with age, oxidative stress may be responsible for the age-dependent sperm DNA damage. The exposing of reproductive organs in older men to oxidative stress for a long time may produce more DNA-damaged spermatozoa than youngers. Examining the sperm chromatin quality in testicular cancer and Hodgkin’s lymphoma patients prior to chemotherapy demonstrated the high incidence of DNA damage and low compaction in spermatozoa at the time of diagnosis. In chemotherapy cycles with genotoxic agents in cancer patients, an increase in sperm DNA damage was shown after treatment. In overall, those factors occurring during the prenatal or the adult life alter the distribution of proteins associated with sperm chromatin induce changes in germ cells which can be detected in infertile patients.
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References
1. Godde JS, Ura K. Dynamic alterations of linker histone variants during development. Int J Dev Biol 2009; 53: 215-224. [DOI:10.1387/ijdb.082644jg]
2. Gan H, Cai T, Lin X, Wu Y, Wang X, Yang F, et al. Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis. Mol Cell Proteom 2013; 12: 1144-1157. [DOI:10.1074/mcp.M112.020123]
3. Erenpreiss J, Spano M, Erenpreisa J, Bungum M, Giwercman A. Sperm chromatin structure and male fertility: biological and clinical aspects. Asian J Androl 2006; 8: 11-29. [DOI:10.1111/j.1745-7262.2006.00112.x]
4. Zini A, Sigman M. Are tests of sperm DNA damage clinically useful? Pros and cons. J Androl 2009; 30: 219-229. [DOI:10.2164/jandrol.108.006908]
5. Machev N, Gosset P, Viville S. Chromosome abnormalities in sperm from infertile men with normal somatic karyotypes: teratozoospermia. Cytogen Genome Res 2005; 111: 352-357. [DOI:10.1159/000086910]
6. Zini A, Libman J. Sperm DNA damage: clinical significance in the era of assisted reproduction. Canadian Med Assoc J 2006; 175: 495-500. [DOI:10.1503/cmaj.060218]
7. Larson-Cook KL, Brannian JD, Hansen KA, Kasperson KM, Aamold ET, Evenson DP. Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay. Fertil Steril 2003; 80: 895-902. [DOI:10.1016/S0015-0282(03)01116-6]
8. Giwercman A, Richthoff J, Hjøllund H, Bonde JP, Jepson K, Frohm B, et al. Correlation between sperm motility and sperm chromatin structure assay parameters. Fertil Steril 2003; 80: 1404-1412. [DOI:10.1016/S0015-0282(03)02212-X]
9. Sakkas D, Urner F, Bianchi P, Bizzaro D, Wagner I, Jaquenoud N, et al. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod 1996; 11: 837-843. [DOI:10.1093/oxfordjournals.humrep.a019263]
10. Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod 1999; 4: 31-37. [DOI:10.1530/ror.0.0040031]
11. Esteves SC, Agarwal A. Novel concepts in male infertility. Int Braz J Urol 2011; 37: 5-15. [DOI:10.1590/S1677-55382011000100002]
12. Gharagozloo P, Aitken RJ. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Hum Reprod 2011: 1628-1640. [DOI:10.1093/humrep/der132]
13. Talebi A, Moein M, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245-251. [DOI:10.1111/j.1439-0272.2008.00852.x]
14. Meetoo D, McGovern P, Safadi R. An epidemiological overview of diabetes across the world. Br J Nurs 2007; 16: .1002-1007
15. Brucker-Davis F, Thayer K, Colborn T. Significant effects of mild endogenous hormonal changes in humans: considerations for low-dose testing. Environment Health Perspect 2001; 109 (Suppl.): 21. [DOI:10.1289/ehp.01109s121]
16. Ding G-L, Liu Y, Liu M-E, Pan J-X, Guo M-X, Sheng J-Z, et al. The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J Androl 2015; 17: 948-953. [DOI:10.4103/1008-682X.150844]
17. Solomon H, Man J, Jackson G. Erectile dysfunction and the cardiovascular patient: endothelial dysfunction is the common denominator. Heart 2003; 89: 251-253. [DOI:10.1136/heart.89.3.251]
18. Lewis RW, Fugl‐Meyer KS, Bosch R, Fugl‐Meyer AR, Laumann EO, Lizza E, et al. Epidemiology/risk factors of sexual dysfunction. J Sex Med 2004; 1: 35-39. [DOI:10.1111/j.1743-6109.2004.10106.x]
19. Miralles-Garcia J, Garcia-Diez L. Specific aspects of erectile dysfunction in endocrinology. Int J Impotence Res 2004; 16: S10-S12. [DOI:10.1038/sj.ijir.3901237]
20. Pourentezari M, Talebi A, Mangoli E, Anvari M, Rahimipour M. Additional deleterious effects of alcohol consumption on sperm parameters and DNA integrity in diabetic mice. Andrologia 2016; 48: .564-569
21. Mangoli E, Talebi AR, Anvari M, Pourentezari M. Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice. Iran J Reprod Med 2013; 11: 53.
22. Talebi AR, Mangoli E, Nahangi H, Anvari M, Pourentezari M, Halvaei I. Vitamin C attenuates detrimental effects of diabetes mellitus on sperm parameters, chromatin quality and rate of apoptosis in mice. Eur J Obstet Gynecol Reprod Biol 2014; 181: 32-36. [DOI:10.1016/j.ejogrb.2014.07.007]
23. Mangoli E, Pourentezari M, Anvari M, Talebi A, Nahangi H. The improvement of sperm parameters and chromatin quality by vitamin C. Researcher 2012; 4: 43-49.
24. Agbaje I, Rogers D, McVicar C, McClure N, Atkinson A, Mallidis C, et al. Insulin dependant diabetes mellitus: implications for male reproductive function. Hum Reprod 2007; 22: 1871-1877. [DOI:10.1093/humrep/dem077]
25. Rama Raju G, Jaya Prakash G, Murali Krishna K, Madan K, Siva Narayana T, Ravi Krishna C. Noninsulin‐dependent diabetes mellitus: effects on sperm morphological and functional characteristics, nuclear DNA integrity and outcome of assisted reproductive technique. Andrologia 2012; 44: 490-498. [DOI:10.1111/j.1439-0272.2011.01213.x]
26. Werthman P, Wixon R, Kasperson K, Evenson DP. Significant decrease in sperm deoxyribonucleic acid fragmentation after varicocelectomy. Fertil Steril 2008; 90: 1800-1804. [DOI:10.1016/j.fertnstert.2006.09.019]
27. Lin J, Dhabuwala C, Li H. The role of apoptosis in infertile men with varicoceles: Is the FAS system implicated? Fertil Steril 2001; 76 )Suppl.): S197.
28. Moustafa MH, Sharma RK, Thornton J, Mascha E, Abdel‐Hafez MA, Thomas AJ, et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod 2004; 19: 129-138. [DOI:10.1093/humrep/deh024]
29. Gual-Frau J, Abad C, Amengual MJ, Hannaoui N, Checa MA, Ribas-Maynou J, et al. Oral antioxidant treatment partly improves integrity of human sperm DNA in infertile grade I varicocele patients. Hum Fertil 2015; 18: 225-229. [DOI:10.3109/14647273.2015.1050462]
30. Smith R, Kaune H, Parodi D, Madariaga M, Ríos R, Morales I, et al. Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Hum Reprod 2006; 21: 986-993. [DOI:10.1093/humrep/dei429]
31. Enciso M, Muriel L, Fernández JL, Goyanes V, Segrelles E, Marcos M, et al. Infertile men with varicocele show a high relative proportion of sperm cells with intense nuclear damage level, evidenced by the sperm chromatin dispersion test. J Androl 2006; 27: 106-111. [DOI:10.2164/jandrol.05115]
32. Telli O, Sarici H, Kabar M, Ozgur BC, Resorlu B, Bozkurt S. Does varicocelectomy affect DNA fragmentation in infertile patients? Indian J Urol 2015; 31: 116-119. [DOI:10.4103/0970-1591.152811]
33. Mohammed E-EM, Mosad E, Zahran AM, Hameed DA, Taha EA, Mohamed MA. Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity? Adv Urol 2015; 2015: 814150.
34. Ghanaie MM, Asgari SA, Dadrass N, Allahkhah A, Iran-Pour E, Safarinejad MR. Effects of varicocele repair on spontaneous first trimester miscarriage: a randomized clinical trial. Urol J 2012; 9: 505-513.
35. Öztürk Mİ, Koca O, Keleş MO, Yılmaz S, Karaman MI. Increased sperm DNA damage in experimental rat varicocele model and the beneficial effect of varicocelectomy. Int J Fertil Steril 2012; 6: 95-100. [DOI:10.1016/S1569-9056(13)60250-X]
36. Restelli AE, Bertolla RP, Spaine DM, Miotto A, Borrelli M, Cedenho AP. Quality and functional aspects of sperm retrieved through assisted ejaculation in men with spinal cord injury. Fertil Steril 2009; 91: 819-825. [DOI:10.1016/j.fertnstert.2007.12.060]
37. Utida C, Truzzi JC, Bruschini H, Simonetti R, Cedenho AP, Srougi M, et al. Male infertility in spinal cord trauma. Int Braz J Urol 2005; 31: 375-383. [DOI:10.1590/S1677-55382005000400013]
38. da Silva BF, Borrelli M, Fariello RM, Restelli AE, Del Giudice PT, Spaine DM, et al. Is sperm cryopreservation an option for fertility preservation in patients with spinal cord injury-induced anejaculation? Fertil Steril 2010; 94: 564-573. [DOI:10.1016/j.fertnstert.2009.03.022]
39. Hirsch IH, Huang B, Chancellor MB, Rivas DA, Salzman SK, Jost LK, et al. Spermatogenesis in early and chronic phases of experimental spinal cord injury in the rodent model. J Androl 1999; 20: 63-71.
40. Momen MN, Fahmy I, Amer M, Arafa M, Zohdy W, Naser TA. Semen parameters in men with spinal cord injury: changes and aetiology. Asian J Androl 2007; 9: 684-689. [DOI:10.1111/j.1745-7262.2007.00277.x]
41. Salsabili N, Mehrsai A, Jalalizadeh B, Pourmand G, Jalaie S. Correlation of sperm nuclear chromatin condensation staining method with semen parameters and sperm functional tests in patients with spinal cord injury, varicocele, and idiopathic infertility. Urol J 2009; 3: 32-37.
42. Talebi AR, Khalili MA, Hossaini A. Assessment of nuclear DNA integrity of epididymal spermatozoa following experimental chronic spinal cord injury in the rat. Int J Androl 2007; 30: 163-169. [DOI:10.1111/j.1365-2605.2006.00736.x]
43. Brackett NL, Ibrahim E, Grotas JA, Aballa TC, Lynne CM. Higher sperm DNA damage in semen from men with spinal cord injuries compared with controls. J Androl 2008; 29: 93-99. [DOI:10.2164/jandrol.107.003574]
44. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9: 331-345. [DOI:10.1093/humupd/dmg027]
45. Spanò M, Bonde JP, Hjøllund HI, Kolstad HA, Cordelli E, Leter G, et al. Sperm chromatin damage impairs human fertility. Fertil Steril 2000; 73: 43-50. [DOI:10.1016/S0015-0282(99)00462-8]
46. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674-677. [DOI:10.1016/S0015-0282(00)01796-9]
47. Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 2003; 18: 1023-1028. [DOI:10.1093/humrep/deg228]
48. Høst E, Lindenberg S, Smidt-Jensen S. The role of DNA strand breaks in human spermatozoa used for IVF and ICSI. Acta Obstet Gynecol Scand 2000; 79: 559-563. [DOI:10.1080/j.1600-0412.2000.079007559.x]
49. Evenson D, Wixon R. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reprod Biomed Online 2006; 12: 466-472. [DOI:10.1016/S1472-6483(10)62000-7]
50. de Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod 1995; 10 (suppl.): 15-21. [DOI:10.1093/humrep/10.suppl_1.15]
51. Patki P, Woodhouse J, Hamid R, Craggs M, Shah J. Effects of spinal cord injury on semen parameters. J Spinal Cord Med 2008; 31: 27-32. [DOI:10.1080/10790268.2008.11753977]
52. Mahfouz R, Sharma R, Thiyagarajan A, Kale V, Gupta S, Sabanegh E, et al. Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species. Fertil Steril 2010; 94: 2141-2146. [DOI:10.1016/j.fertnstert.2009.12.030]
53. Talebi AR, Khalili MA, Vahidi S, Ghasemzadeh J, Tabibnejad N. Sperm chromatin condensation, DNA integrity, and apoptosis in men with spinal cord injury. J Spinal Cord Med 2013; 36: 140-146. [DOI:10.1179/2045772312Y.0000000055]
54. Salsabili N, Mehrsai A, Jalaie S. Concentration of blood and seminal plasma elements and their relationships with semen parameters in men with spinal cord injury. Andrologia 2009; 41: 24-28. [DOI:10.1111/j.1439-0272.2008.00885.x]
55. Bray F, Richiardi L, Ekbom A, Pukkala E, Cuninkova M, Møller H. Trends in testicular cancer incidence and mortality in 22 European countries: continuing increases in incidence and declines in mortality. Int J Cancer 2006; 118: 3099-3111. [DOI:10.1002/ijc.21747]
56. Walsh TJ, Grady RW, Porter MP, Lin DW, Weiss NS. Incidence of testicular germ cell cancers in US children: SEER program experience 1973 to 2000. Urology 2006; 68: 402-405. [DOI:10.1016/j.urology.2006.02.045]
57. Paoli D, Gallo M, Rizzo F, Spanò M, Leter G, Lombardo F, et al. Testicular cancer and sperm DNA damage: short‐and long‐term effects of antineoplastic treatment. Andrology 2015; 3: 122-128. [DOI:10.1111/j.2047-2927.2014.00250.x]
58. Ghezzi M, Berretta M, Bottacin A, Palego P, Sartini B, Cosci I, et al. Impact of Bep or Carboplatin Chemotherapy on Testicular Function and Sperm Nucleus of Subjects with Testicular Germ Cell Tumor. Front Pharmacol 2016; 7: 122. [DOI:10.3389/fphar.2016.00122]
59. Fossa SD, De Angelis P, Kraggerud SM, Evenson D, Theodorsen L, Clausen OP. Prediction of posttreatment spermatogenesis in patients with testicular cancer by flow cytometric sperm chromatin structure assay. J Urol 1998; 160: 947-948. [DOI:10.1016/S0022-5347(01)62841-6]
60. Maselli J, Hales BF, Chan P, Robaire B. Exposure to bleomycin, etoposide, and cis-platinum alters rat sperm chromatin integrity and sperm head protein profile. Biol Reprod 2012; 86: 166. [DOI:10.1095/biolreprod.111.098616]
61. O'Flaherty C, Vaisheva F, Hales B, Chan P, Robaire B. Characterization of sperm chromatin quality in testicular cancer and Hodgkin's lymphoma patients prior to chemotherapy. Hum Reprod 2008; 23: 1044-1052. [DOI:10.1093/humrep/den081]
62. Morris ID. Sperm DNA damage and cancer treatment. Int J Androl 2002; 25: 255-261. [DOI:10.1046/j.1365-2605.2002.00372.x]
63. Tanrikut C, Feldman AS, Altemus M, Paduch DA, Schlegel PN. Adverse effect of paroxetine on sperm. Fertil Steril 2010; 94: 1021-1026. [DOI:10.1016/j.fertnstert.2009.04.039]
64. Chan P, Robaire B. Cancer in Males: Implications for Sperm Quality, Fertility, and Progeny Outcome. Sperm Chromatin: Springer; 2011: 351-360.
65. Kobayashi H, Larson K, Sharma RK, Nelson DR, Evenson DP, Toma H, et al. DNA damage in patients with untreated cancer as measured by the sperm chromatin structure assay. Fertil Steril 2001; 75: 469-475. [DOI:10.1016/S0015-0282(00)01740-4]
66. O'flaherty CM, Chan PT, Hales BF, Robaire B. Sperm chromatin structure components are differentially repaired in cancer survivors. J Androl 2012; 33: 629-636. [DOI:10.2164/jandrol.111.015388]
67. Ochsendorf F. Infections in the male genital tract and reactive oxygen species. Hum Reprod Update 1999; 5: 399-420. [DOI:10.1093/humupd/5.5.399]
68. Cocuzza M, Sikka SC, Athayde KS, Agarwal A. Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis. Int Braz J Urol 2007; 33: 603-621. [DOI:10.1590/S1677-55382007000500002]
69. Potts JM, Sharma R, Pasqualotto F, Nelson D, Hall G, Agarwal A. Association of Ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. J Urol 2000; 163: 1775-1778. [DOI:10.1016/S0022-5347(05)67540-4]
70. Peng HW, Su TS, Han SH, Ho CK, Ho CH, Ching KN, et al. Assessment of HBV persistent infection in an adult population in Taiwan. J Medical Virol 1988; 24: 405-412. [DOI:10.1002/jmv.1890240407]
71. Huang J-M, Huang T-H, Qiu H-Y, Fang X-W, Zhuang T-G, Liu H-X, et al. Effects of hepatitis B virus infection on human sperm chromosomes. World J Gastroenterol 2003; 9: 736-740. [DOI:10.3748/wjg.v9.i4.736]
72. Kang X, Xie Q, Zhou X, Li F, Huang J, Liu D, et al. Effects of hepatitis B virus S protein exposure on sperm membrane integrity and functions. PLoS One 2012; 7: e33471. [DOI:10.1371/journal.pone.0033471]
73. Foresta C, Garolla A, Zuccarello D, Pizzol D, Moretti A, Barzon L, et al. Human papillomavirus found in sperm head of young adult males affects the progressive motility. Fertil Steril 2010; 93: 802-806. [DOI:10.1016/j.fertnstert.2008.10.050]
74. Rintala M, Greénman S, Pöllänen P, Suominen J, Syrjänen S. Detection of high-risk HPV DNA in semen and its association with the quality of semen. Int J STD AIDS 2004; 15: 740-743. [DOI:10.1258/0956462042395122]
75. Kaspersen MD, Bungum M, Fedder J, Bonde J, Larsen PB, J Ingerslev H, et al. No increased sperm DNA fragmentation index in semen containing human papillomavirus or herpesvirus. Andrology 2013; 1: 361-364. [DOI:10.1111/j.2047-2927.2013.00067.x]
76. Sasikumar S, Dakshayani D, Sarasa D. An Investigation of DNA Fragmentation and Morphological Changes caused by Bacteria and Fungi in Human Spermatozoa. Int J Curr Microbiol App Sci 2013; 2: 84-96.
77. Villegas J, Schulz M, Soto L, Sanchez R. Bacteria induce expression of apoptosis in human spermatozoa. Apoptosis 2005; 10: 105-110. [DOI:10.1007/s10495-005-6065-8]
78. Gallegos G, Ramos B, Santiso R, Goyanes V, Gosálvez J, Fernández JL. Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma. Fertil Steril 2008; 90: 328-334. [DOI:10.1016/j.fertnstert.2007.06.035]
79. Safarinejad MR. Sperm DNA damage and semen quality impairment after treatment with selective serotonin reuptake inhibitors detected using semen analysis and sperm chromatin structure assay. J Urol 2008; 180: 2124-2128. [DOI:10.1016/j.juro.2008.07.034]
80. Pollack MH, Reiter S, Hammerness P. Genitourinary and sexual adverse effects of psychotropic medication. Int J Psychiatr Med 1992; 22: 305-327. [DOI:10.2190/P60R-PLED-TL09-TUEN]
81. Koyuncu H, Serefoglu E, Yencilek E, Atalay H, Akbas N, Sarıca K. Escitalopram treatment for premature ejaculation has a negative effect on semen parameters. Int J Impotence Res 2011; 23: 257-261. [DOI:10.1038/ijir.2011.35]
82. Khazaie H, Rezaie L, Payam NR, Najafi F. Antidepressant-induced sexual dysfunction during treatment with fluoxetine, sertraline and trazodone; a randomized controlled trial. Gen Hosp Psychiatry 2015; 37: 40-45. [DOI:10.1016/j.genhosppsych.2014.10.010]
83. Khin NA, Kronstein PD, Yang P, Ishida E, Hung HJ, Mathis MV, et al. Regulatory and scientific issues in studies to evaluate sexual dysfunction in antidepressant drug trials. J Clin Psychiatry 2015; 76: 1060-1063. [DOI:10.4088/JCP.14cs09700]
84. Lim SS, Vos T, Flaxman AD, Danaei G, Shibuya K, Adair-Rohani H, et al. A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2013; 380: 2224-2260. [DOI:10.1016/S0140-6736(12)61766-8]
85. Van Thiel D, Gavaler JS, Cobb CF, Graham TO. Ethanol, a Leydig cell toxin: evidence obtained in vivo and in vitro. Pharmacol Biochem Behav 1983; 18: 317-323. [DOI:10.1016/0091-3057(83)90193-4]
86. Salonen I, Eriksson C. Penetration of ethanol into the male reproductive tract. Alcoholism: Clin Exp Res 1989; 13: 746-751. [DOI:10.1111/j.1530-0277.1989.tb00414.x]
87. Komiya A, Kato T, Kawauchi Y, Watanabe A, Fuse H. Clinical factors associated with sperm DNA fragmentation in male patients with infertility. Sci World J 2014; 2014: 868303. [DOI:10.1155/2014/868303]
88. Talebi AR, Sarcheshmeh AA, Khalili MA, Tabibnejad N. Effects of ethanol consumption on chromatin condensation and DNA integrity of epididymal spermatozoa in rat. Alcohol 2011; 45: 403-409. [DOI:10.1016/j.alcohol.2010.10.005]
89. Rahimipour M, Talebi AR, Anvari M, Sarcheshmeh AA, Omidi M. Effects of different doses of ethanol on sperm parameters, chromatin structure and apoptosis in adult mice. Eur J Obstet Gynecol Reprod Biol 2013; 170: 423-428. [DOI:10.1016/j.ejogrb.2013.06.038]
90. Eid NA, Shibata MA, Ito Y, Kusakabe K, Hammad H, Otsuki Y. Involvement of Fas system and active caspases in apoptotic signalling in testicular germ cells of ethanol‐treated rats. Int J Androl 2002; 25: 159-167. [DOI:10.1046/j.1365-2605.2002.00341.x]
91. Ouko LA, Shantikumar K, Knezovich J, Haycock P, Schnugh DJ, Ramsay M. Effect of Alcohol Consumption on CpG Methylation in the Differentially Methylated Regions of H19 and IG‐DMR in Male Gametes- Implications for Fetal Alcohol Spectrum Disorders. Alcoholism: Clin Exp Res 2009; 33: 1615-1627. [DOI:10.1111/j.1530-0277.2009.00993.x]
92. Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage. Alcohol Res Health 2003; 27: 277-284.
93. Amanvermez R, Demir S, Tunçel ÖK, Alvur M, Agar E. Alcohol-induced oxidative stress and reduction in oxidation by ascorbate/L-cys/L-met in the testis, ovary, kidney, and lung of rat. Adv Ther 2005; 22: 548-558. [DOI:10.1007/BF02849949]
94. Cacciola G, Chioccarelli T, Ricci G, Meccariello R, Fasano S, Pierantoni R, et al. The endocannabinoid system in vertebrate male reproduction: a comparative overview. Mol Cell Endocrinol 2008; 286: S24-S30. [DOI:10.1016/j.mce.2008.01.004]
95. Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ, Agarwal A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril 2000; 73: 459-464. [DOI:10.1016/S0015-0282(99)00567-1]
96. Song B-J, Moon K-H, Upreti VV, Eddington ND, Lee IJ. Mechanisms of MDMA (ecstasy)-induced oxidative stress, mitochondrial dysfunction, and organ damage. Curr Pharm Biotechnol 2010; 11: 434-443. [DOI:10.2174/138920110791591436]
97. Barenys M, Macia N, Camps L, de Lapuente J, Gomez-Catalan J, Gonzalez-Linares J, et al. Chronic exposure to MDMA (ecstasy) increases DNA damage in sperm and alters testes histopathology in male rats. Toxicol Lett 2009; 191: 40-46. [DOI:10.1016/j.toxlet.2009.08.002]
98. Battista N, Pasquariello N, Di Tommaso M, Maccarrone M. Interplay between endocannabinoids, steroids and cytokines in the control of human reproduction. J Neuroendocrinol 2008; 20: 82-89. [DOI:10.1111/j.1365-2826.2008.01684.x]
99. Fronczak CM, Kim ED, Barqawi AB. The insults of illicit drug use on male fertility. J Androl 2012; 33: 515-528. [DOI:10.2164/jandrol.110.011874]
100. Safarinejad MR, Asgari SA, Farshi A, Ghaedi G, Kolahi AA, Iravani S, et al. The effects of opiate consumption on serum reproductive hormone levels, sperm parameters, seminal plasma antioxidant capacity and sperm DNA integrity. Reprod Toxicol 2013; 36: 18-23. [DOI:10.1016/j.reprotox.2012.11.010]
101. Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, Jabs E, et al. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Nat Acad Sci 2006; 103: 9601-9606. [DOI:10.1073/pnas.0506468103]
102. Eskenazi B, Wyrobek AJ, Sloter E, Kidd S, Moore L, Young S, et al. The association of age and semen quality in healthy men. Hum Reprod 2003; 18: 447-454. [DOI:10.1093/humrep/deg107]
103. Pasqualotto FF, Sobreiro BP, Hallak J, Pasqualotto EB, Lucon AM. Sperm concentration and normal sperm morphology decrease and follicle‐stimulating hormone level increases with age. BJU Int 2005; 96: 1087-1091. [DOI:10.1111/j.1464-410X.2005.05806.x]
104. Morris I, Ilott S, Dixon L, Brison D. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod 2002; 17: 990-998. [DOI:10.1093/humrep/17.4.990]
105. Angelopoulou R, Plastira K, Msaouel P. Spermatozoal sensitive biomarkers to defective protaminosis and fragmented DNA. Reprod Biol Endocrinol 2007; 5: 36. [DOI:10.1186/1477-7827-5-36]
106. Youssry M, Ozmen B, Orief Y, Zohni K, Al-Hasani S. Human sperm DNA damage in the context of assisted reproductive techniques. Iran J Reprod Med 2007; 5: 137-150.
107. Moskovtsev SI, Willis J, Mullen JBM. Age-related decline in sperm deoxyribonucleic acid integrity in patients evaluated for male infertility. Fertil Steril 2006; 85: 496-499. [DOI:10.1016/j.fertnstert.2005.05.075]
108. Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 2003; 17: 1195-1214. [DOI:10.1096/fj.02-0752rev]
109. Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem J 1996; 313: 17-29. [DOI:10.1042/bj3130017]
110. Alexeyev MF. Is there more to aging than mitochondrial DNA and reactive oxygen species? FEBS J 2009; 276: 5768-5787. [DOI:10.1111/j.1742-4658.2009.07269.x]
111. Carrell DT. Paternal influences on human reproductive success: Cambridge University Press; 2013. [DOI:10.1017/CBO9781139169349]
112. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000; 15: 1338-1344. [DOI:10.1093/humrep/15.6.1338]
113. Cocuzza M, Athayde KS, Agarwal A, Sharma R, Pagani R, Lucon AM, et al. Age-related increase of reactive oxygen species in neat semen in healthy fertile men. Urology 2008; 71: 490-494. [DOI:10.1016/j.urology.2007.11.041]
114. Potts J, Pasqualotto F. Seminal oxidative stress in patients with chronic prostatitis. Andrologia 2003; 35: 304-308. [DOI:10.1111/j.1439-0272.2003.tb00862.x]
115. de La Rochebrochard E, Thonneau P. Paternal age and maternal age are risk factors for miscarriage; results of a multicentre European study. Hum Reprod 2002; 17: 1649-1656. [DOI:10.1093/humrep/17.6.1649]
116. Schmid T, Eskenazi B, Baumgartner A, Marchetti F, Young S, Weldon R, et al. The effects of male age on sperm DNA damage in healthy non-smokers. Hum Reprod 2007; 22: 180-187. [DOI:10.1093/humrep/del338]
117. Azam S, Hadi N, Khan NU, Hadi SM. Antioxidant and prooxidant properties of caffeine, theobromine and xanthine. Med Sci Monitor 2003; 9: BR325-BR330.
118. Sarkaria JN, Busby EC, Tibbetts RS, Roos P, Taya Y, Karnitz LM, et al. Inhibition of ATM and ATR kinase activities by the radiosensitizing agent, caffeine. Cancer Res 1999; 59: 4375-4382.
119. Dupont C, Faure C, Sermondade N, Boubaya M, Eustache F, Clément P, et al. Obesity leads to higher risk of sperm DNA damage in infertile patients. Asian J Androl 2013; 15: 622-625. [DOI:10.1038/aja.2013.65]
120. Kort HI, Massey JB, Elsner CW, Mitchell‐Leef D, Shapiro DB, Witt MA, et al. Impact of body mass index values on sperm quantity and quality. J Androl 2006; 27: 450-452. [DOI:10.2164/jandrol.05124]
121. Tamburrino L, Marchiani S, Montoya M, Elia Marino F, Natali I, Cambi M, et al. Mechanisms and clinical correlates of sperm DNA damage. Asian J Androl 2012; 14: 24-31. [DOI:10.1038/aja.2011.59]
122. Gandhi G, Kaur G. Assessment of DNA damage in obese individuals. Res J Biol 2012; 2: 37-44.
123. Tunc O, Bakos H, Tremellen K. Impact of body mass index on seminal oxidative stress. Andrologia 2011; 43: 121-128. [DOI:10.1111/j.1439-0272.2009.01032.x]
124. Rybar R, Kopecka V, Prinosilova P, Markova P, Rubes J. Male obesity and age in relationship to semen parameters and sperm chromatin integrity. Andrologia 2011; 43: 286-291. [DOI:10.1111/j.1439-0272.2010.01057.x]
125. Momeni HR, Eskandari N. Effect of vitamin E on sperm parameters and DNA integrity in sodium arsenite-treated rats. Iran J Reprod Med 2012; 10: 249-256.
126. Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. Etiologies of sperm oxidative stress. Int J Reprod BioMed 2016; 14: 231-240.
127. Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004; 8: 616-627. [DOI:10.1016/S1472-6483(10)61641-0]
128. Silver EW, Eskenazi B, Evenson DP, Block G, Young S, Wyrobek AJ. Effect of antioxidant intake on sperm chromatin stability in healthy nonsmoking men. J Androl 2005; 26: 550-556. [DOI:10.2164/jandrol.04165]
129. Sen S, Chakraborty R. The role of antioxidants in human health. Oxidative stress: diagnostics, prevention, and therapy. 2011;1083:1-37. [DOI:10.1021/bk-2011-1083.ch001]
130. Wyrobek AJ. Methods and concepts in detecting abnormal reproductive outcomes of paternal origin. Reprod Toxicol 1993; 7: 3-16. [DOI:10.1016/0890-6238(93)90064-E]
131. Gupta C. Reproductive malformation of the male offspring following maternal exposure to estrogenic chemicals. Proc Soc Exp Biol Med 2000; 224: 61-68. [DOI:10.1046/j.1525-1373.2000.22402.x]
132. Meeker JD, Hauser R. Exposure to polychlorinated biphenyls (PCBs) and male reproduction. Syst Biol Reprod Med 2010; 56: 122-131. [DOI:10.3109/19396360903443658]
133. Aktar W, Sengupta D, Chowdhury A. Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip Toxicol 2009; 2: 1-12. [DOI:10.2478/v10102-009-0001-7]
134. Roeleveld N, Bretveld R. The impact of pesticides on male fertility. Curr Opin Obstet Gynecol 2008; 20: 229-233. [DOI:10.1097/GCO.0b013e3282fcc334]
135. Control CfD, Prevention. Chemistry and Toxicology of Cigarette Smoke and Biomarkers of Exposure and Harm. 2010.
136. Eftekhar M, Pourmasumi S, Sabeti P, Mirhosseini F. Relation of Second Hand Smoker and Effect on Pregnancy Outcome and Newborns Parameters. Womens Health Gynecol 2016; 6: 2.
137. Arabi M, Moshtaghi H. Influence of cigarette smoking on spermatozoa via seminal plasma. Andrologia 2005; 37: 119-124. [DOI:10.1111/j.1439-0272.2005.00664.x]
138. Briviba K, Kulling SE, Möseneder J, Watzl B, Rechkemmer G, Bub A. Effects of supplementing a low-carotenoid diet with a tomato extract for 2 weeks on endogenous levels of DNA single strand breaks and immune functions in healthy non-smokers and smokers. Carcinogenesis 2004; 25: 2373-2378. [DOI:10.1093/carcin/bgh249]
139. Künzle R, Mueller MD, Hänggi W, Birkhäuser MH, Drescher H, Bersinger NA. Semen quality of male smokers and nonsmokers in infertile couples. Fertil Steril 2003; 79: 287-291. [DOI:10.1016/S0015-0282(02)04664-2]
140. Calogero A, Polosa R, Perdichizzi A, Guarino F, La Vignera S, Scarfia A, et al. Cigarette smoke extract immobilizes human spermatozoa and induces sperm apoptosis. Reprod Biomed Online 2009; 19: 564-571. [DOI:10.1016/j.rbmo.2009.05.004]
141. Saleh RA, Agarwal A, Sharma RK, Nelson DR, Thomas AJ. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril 2002; 78: 491-499. [DOI:10.1016/S0015-0282(02)03294-6]
142. Potts R, Newbury C, Smith G, Notarianni L, Jefferies T. Sperm chromatin damage associated with male smoking. Mutat Res 1999; 423: 103-111. [DOI:10.1016/S0027-5107(98)00242-5]
143. Yu B, Qi Y, Liu D, Gao X, Chen H, Bai C, et al. Cigarette smoking is associated with abnormal histone-to-protamine transition in human sperm. Fertil Steril 2014; 101: 51-57. [DOI:10.1016/j.fertnstert.2013.09.001]
144. Hamad M, Shelko N, Kartarius S, Montenarh M, Hammadeh M. Impact of cigarette smoking on histone (H2B) to protamine ratio in human spermatozoa and its relation to sperm parameters. Andrology 2014; 2: 666-677. [DOI:10.1111/j.2047-2927.2014.00245.x]
145. Pembrey ME, Bygren LO, Kaati G, Edvinsson S, Northstone K, Sjöström M, et al. Sex-specific, male-line transgenerational responses in humans. Eur J Hum Genet 2006; 14: 159-166. [DOI:10.1038/sj.ejhg.5201538]
146. Olshan AF, Faustman EM. Male-mediated developmental toxicity. Reprod Toxicol 1993; 7: 191-202. [DOI:10.1016/0890-6238(93)90224-U]
147. Mailankot M, Kunnath AP, Jayalekshmi H, Koduru B, Valsalan R. Radio frequency electromagnetic radiation (RF-EMR) from GSM (0.9/1.8 GHz) mobile phones induces oxidative stress and reduces sperm motility in rats. Clinics 2009; 64: 561-565. [DOI:10.1590/S1807-59322009000600011]
148. Falzone N, Huyser C, Becker P, Leszczynski D, Franken DR. The effect of pulsed 900‐MHz GSM mobile phone radiation on the acrosome reaction, head morphometry and zona binding of human spermatozoa. Int J Androl 2011; 34: 20-26. [DOI:10.1111/j.1365-2605.2010.01054.x]
149. Sivani S, Sudarsanam D. Impacts of radio-frequency electromagnetic field (RF-EMF) from cell phone towers and wireless devices on biosystem and ecosystem-a review. Biol Med 2012; 4: 202-216.
150. Adams JA, Galloway TS, Mondal D, Esteves SC, Mathews F. Effect of mobile telephones on sperm quality: A systematic review and meta-analysis. Environment Int 2014; 70: 106-112. [DOI:10.1016/j.envint.2014.04.015]
151. De Rosa M, Zarrilli S, Paesano L, Carbone U, Boggia B, Petretta M, et al. Traffic pollutants affect fertility in men. Hum Reprod 2003; 18: 1055-1061. [DOI:10.1093/humrep/deg226]
152. Boggia B, Carbone U, Farinaro E, Zarrilli S, Lombardi G, Colao A, et al. Effects of working posture and exposure to traffic pollutants on sperm quality. J Endocrinol Invest 2009; 32: 430-434. [DOI:10.1007/BF03346481]
153. Calogero AE, La Vignera S, Condorelli RA, Perdichizzi A, Valenti D, Asero P, et al. Environmental car exhaust pollution damages human sperm chromatin and DNA. J Endocrinol Invest 2011; 34: 139-143. [DOI:10.1007/BF03346722]
154. Rubes J, Selevan SG, Evenson DP, Zudova D, Vozdova M, Zudova Z, et al. Episodic air pollution is associated with increased DNA fragmentation in human sperm without other changes in semen quality. Hum Reprod 2005; 20: 2776-2783. [DOI:10.1093/humrep/dei122]
155. Ji G, Gu A, Zhou Y, Shi X, Xia Y, Long Y, et al. Interactions between exposure to environmental polycyclic aromatic hydrocarbons and DNA repair gene polymorphisms on bulky DNA adducts in human sperm. PLoS One 2010; 5: pii: e13145. [DOI:10.1371/journal.pone.0013145]
156. Rubes J, Rybar R, Prinosilova P, Veznik Z, Chvatalova I, Solansky I, et al. Genetic polymorphisms influence the susceptibility of men to sperm DNA damage associated with exposure to air pollution. Mutat Res 2010; 683: 9-15. [DOI:10.1016/j.mrfmmm.2009.09.010]
157. Pacey AA. Environmental and lifestyle factors associated with sperm DNA damage. Hum Fertil 2010; 13: 189-193. [DOI:10.3109/14647273.2010.531883]
158. Horak S, Polanska J, Widlak P. Bulky DNA adducts in human sperm: relationship with fertility, semen quality, smoking, and environmental factors. Mutat Res 2003; 537: 53-65. [DOI:10.1016/S1383-5718(03)00051-2]
159. Jung A, Schuppe HC. Influence of genital heat stress on semen quality in humans. Andrologia 2007; 39: 203-215. [DOI:10.1111/j.1439-0272.2007.00794.x]
160. 1Banks S, King SA, Irvine DS, Saunders PT. Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa. Reproduction 2005; 129: 505-514 [DOI:10.1530/rep.1.00531]

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