Volume 14, Issue 8 (8-2016)                   IJRM 2016, 14(8): 533-540 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini J, Mardi Mamaghani A, Hosseinifar H, Sadighi Gilani M A, Dadkhah F, Sepidarkish M. The influence of ginger (Zingiber officinale) on human sperm quality and DNA fragmentation: A double-blind randomized clinical trial. IJRM. 2016; 14 (8) :533-540
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-772-en.html
1- Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3- Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , Mahdi.sepidarkish@gmail.com
Abstract:   (738 Views)
Background: Although the effectiveness of ginger as an antioxidant agent has been exploited, little human research has been conducted on its activity on male reproductive functions.
Objective: This study was designed to investigate the effects of ginger (Zingiber officinale) on sperm DNA fragmentation (SDF) in infertile men.
Materials and Methods: This randomized double-blind, placebo-controlled trial with a 1:1 allocation was performed on 100 infertility treatment candidates who were admitted to Royan Institute for Reproductive Biomedicine, Tehran, Iran. Patients were randomly assigned to receive one of two treatments: ginger and placebo. Patients were given a 3-month oral treatment (members received capsules containing 250 mg of ginger powder twice a day in ginger and a placebo in other group). Before and after treatment, standardized semen samples were obtained to determine sperm concentration, motility, and SDF according to World Health Organization.
Results: There was no significant difference between two groups regarding SDF at baseline (53.48. 95%CI: 37.95-69.02) in cases and (56.75, 95%CI: 40.01-73.5) in controls. The average positive percentage of SDF in patients receiving ginger (17.77, 95%CI: 6.16-29.39) was lower compared with placebo (40.54, 95%CI: 23.94-57.13) after three month of treatment (p=0.02). In multivariate analysis, SDF was significantly lower in patients receiving ginger compared with placebo (mean difference: 3.21, 95%CI: 0.78-5.63, p=0.009). There were no significant differences between two groups regarding to semen parameters.
Conclusion: The present study has demonstrated that ginger in a controlled study of efficacy was effective in decreasing SDF in infertile men.
Full-Text [PDF 239 kb]   (143 Downloads) |   |   Full-Text (HTML)  (42 Views)  
Type of Study: Original Article |

References
1. Aitken RJ, De Iuliis GN. Origins and consequences of DNA damage in male germ cells. Reprod Biomed Online 2007; 14: 727-733. [DOI:10.1016/S1472-6483(10)60676-1]
2. Gonzalez-Marin C, Gosalvez J, Roy R. Types, causes, detection and repair of DNA fragmentation in animal and human sperm cells. Int J Mol Sci 2012; 13: 14026-14052. [DOI:10.3390/ijms131114026]
3. Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl 2005; 26: 349-353. [DOI:10.2164/jandrol.04146]
4. Henkel R, Kierspel E, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, et al. DNA fragmentation of spermatozoa and assisted reproduction technology. Reprod Biomed Online 2003; 7: 477-484. [DOI:10.1016/S1472-6483(10)61893-7]
5. Lewis SEM. Sperm DNA fragmentation and base oxidation. Genetic Damage in Human Spermatozoa, Springer; 2014: 103-116. [DOI:10.1007/978-1-4614-7783-9_7]
6. Benchaib M, Braun Vr, Lornage J, Hadj S, Salle B, Lejeune H, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 2003; 18: 1023-1028. [DOI:10.1093/humrep/deg228]
7. Sergerie M, Laforest G, Bujan L, Bissonnette F, Bleau G. Sperm DNA fragmentation: threshold value in male fertility. Hum Reprod 2005; 20: 3446-3451. [DOI:10.1093/humrep/dei231]
8. Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol 2009; 16: 449-457. [DOI:10.1111/j.1442-2042.2009.02280.x]
9. Menezo Y, Evenson D, Cohen M, Dale B. Effect of antioxidants on sperm genetic damage. Genetic Damage in Human Spermatozoa, Springer; 2014: 173. [DOI:10.1007/978-1-4614-7783-9_11]
10. Ross C, Morriss A, Khairy M, Khalaf Y, Braude P, Coomarasamy A, et al. A systematic review of the effect of oral antioxidants on male infertility. Reprod Biomed Online 2014; 20: 711-723. [DOI:10.1016/j.rbmo.2010.03.008]
11. Lim TK. Zingiber officinale. Edible Medicinal and Non-Medicinal Plants, Springer; 2016: 469. [DOI:10.1007/978-3-319-26065-5_21]
12. Akhlaghi A, Ahangari YJ, Navidshad B, Pirsaraei ZA, Zhandi M, Deldar H, et al. Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Cobb 500 breeder roosters fed diets containing dried ginger rhizomes (Zingiber officinale). Poultry Sci 2014; 93: 1236-1244. [DOI:10.3382/ps.2013-03617]
13. Bordbar H, Esmaeilpour T, Dehghani F, Panjehshahin MR. Stereological study of the effect of ginger's alcoholic extract on the testis in busulfan-induced infertility in rats. Iran J Reprod Med 2013; 11: 467-472.
14. Busman H, Kanedi M. Testicular Function of Rats Treated with Water Extract of Red Ginger (Zingiber officinale var. rubrum) Combined with Zinc. J Food Nutr Res 2016; 4: 157-162.
15. Hafez DA. Effect of extracts of ginger roots and cinnamon bark on fertility of male diabetic rats. J Am Sci 2014; 6: 940-947.
16. Ikpeme EV, Udensi OU, Ekerette EE, Okon UH. Potential of Ginger (Zingiber officinale) Rhizome and Watermelon (Citrullus lanatus) Seeds in Mitigating Aspartame-Induced Oxidative Stress in Rat Model. Res J Med Plant 2016; 10: 55-66. [DOI:10.3923/rjmp.2016.55.66]
17. Mohammadi F, Nikzad H, Taghizadeh M, Taherian A, Azami-Tameh A, Hosseini SM, et al. Protective effect of Zingiber officinale extract on rat testis after cyclophosphamide treatment. Andrologia 2014; 46: 680-686. [DOI:10.1111/and.12135]
18. Morakinyo AO, Achema PU, Adegoke OA. Effect of Zingiber officinale (Ginger) on sodium arsenite-induced reproductive toxicity in male rats. Afr J Biomed Res 2013; 13: 39-45.
19. Mares AK, Abid W, Najam WS. The effect of Ginger on semen parameters and serum FSH, LH & testosterone of infertile men. Tikrit Med J 2012; 18: 322.
20. World Medical A. World Medical Association Declaration of Helsinki. Ethical principles for medical research involving human subjects. Bulletin of the World Health Organization; 2001: 373.
21. World Health O. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press; 1999.
22. Loo DT. TUNEL assay. In Situ Detection of DNA Damage. Springer; 2002: 21. [DOI:10.1385/1-59259-179-5:21]
23. Abad C, Amengual MJ, Gosálvez J, Coward K, Hannaoui N, Benet J, et al. Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulations of sperm with highly degraded DNA. Andrologia 2013; 45: 211-216. [DOI:10.1111/and.12003]
24. Gual-Frau J, Abad C, Amengual MJ, Hannaoui N, Checa MA, Ribas-Maynou J, et al. Oral antioxidant treatment partly improves integrity of human sperm DNA in infertile grade I varicocele patients. Hum Fertil 2015; 18: 225-229. [DOI:10.3109/14647273.2015.1050462]
25. Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, et al. Investigation on the origin of sperm DNA fragmentation: role of apoptosis, immaturity and oxidative stress. Mol Med 2015; 21: 109-122. [DOI:10.2119/molmed.2014.00158]
26. Palacio C, Mooradian AD. Clinical trials and antioxidant outcomes. Oxidative Stress and Antioxidant Protection: The Science of Free Radical Biology and Disease. 2016:493-506.
27. Hegazy AMS, Mosaed MM, Elshafey SH, Bayomy NA. 6-gingerol ameliorates gentamicin induced renal cortex oxidative stress and apoptosis in adult male albino rats. Tissue Cell 2016; 48: 208-216. [DOI:10.1016/j.tice.2016.03.006]
28. Gharagozloo P, Gutieerrez-Adan A, Champroux A, Noblanc A, Kocer A, Calle A, et al. A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models. Hum Reprod 2016; 31: 252-262. [DOI:10.1093/humrep/dev302]
29. Osman A, Alsomait H, Seshadri S, El-Toukhy T, Khalaf Y. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis. Reprod Biomed Online 2015; 30: 120-127. [DOI:10.1016/j.rbmo.2014.10.018]
30. Toader OR. Study of the effects of Zingiber officinale (ginger) on spermatogenesis in mice. Ann West Univ Timisoara Ser Biol 2014; 17: 145-152.
31. Ali DA, Abdeen AM, Ismail MF, Mostafa MA. Histological, ultrastructural and immunohistochemical studies on the protective effect of ginger extract against cisplatin-induced nephrotoxicity in male rats. Toxicol Ind Health 2015: 31: 869-880. [DOI:10.1177/0748233713483198]
32. Zahedi A, Khaki A. Recovery effect of Zingiber officinale on testis tissue after treatment with gentamicin in rats. J Med Plants Res 2014; 8: 288-291. [DOI:10.5897/JMPR11.312]
33. Nasimi P, Vahdati A, Tabandeh MR, Khatamsaz S. Cytoprotective and antiapoptotic effects of Satureja khuzestanica essential oil against busulfan mediated sperm damage and seminiferous tubules destruction in adult male mice. Andrologia 2016; 48: 74-81. [DOI:10.1111/and.12421]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb