Volume 14, Issue 3 (3-2016)                   IJRM 2016, 14(3): 199-204 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Talebi A R, Fesahat F, Mangoli E, Ghasemzadeh J, Nayeri M, Sadeghian-Nodoshan F. Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions. IJRM. 2016; 14 (3) :199-204
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-731-en.html
1- Infertility Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Biology, Islamic Azad University of Ashkezar, Ashkezar, Iran
3- Stem Cell Biology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , fsadeghian@gmail.com
Abstract:   (716 Views)
Background: Etiology of more than half of Recurrent Spontaneous Abortion. The etiology of more than 50 percent of Recurrent Spontaneous Abortions (RSA) cases has been remained unexplained. It is supposed that RSA may have "paternal effect" due to supply 50% of embryonic genomic content by male gamete.
Objective: The aim of present study was to evaluate the role of sperm apoptosis and protamine deficiency at same time in RSA cases.
Materials and Methods: Forty fertile (control) and 40 unfertile men with RSA (case) were enrolled in this case-control study. Semen analysis was performed in accordance with WHO criteria and sperm apoptosis and protamine deficiency were evaluated by cell apoptosis detection kit and chromomycin A3, respectively.
Results: Results showed significant different between normal morphology and total motility in two groups. Case group had higher percentage of spermatozoa with protamine deficiency and apoptosis compared to controls significantly.
Conclusion: Our results showed that in cases of RSA, in addition to abnormal sperm parameters, we have a high percentage of spermatozoa with protamine deficiency and apoptosis and these two anomalies may consider as important causes of idiopathic recurrent abortions. It should be advised that sperm chromatin and DNA examinations are useful tools in the process of RSA treatments.
Full-Text [PDF 121 kb]   (158 Downloads) |   |   Full-Text (HTML)  (27 Views)  
Type of Study: Original Article |

References
1. McVicar CM, McClure N, Williamson K, Dalzell LH, Lewis SE. Incidence of Fas positivity and deoxyribonucleic acid double-stranded breaks in human ejaculated sperm. Fertil Steril 2004; 1: 767-774. [DOI:10.1016/j.fertnstert.2003.10.013]
2. Acharyya S, Kanjilal S, Bhattacharyya AK. Does human sperm nuclear DNA integrity affect embryo quality? Indian J Exp Biol 2005; 43: 1016-1022.
3. Morris ID, Ilott S, Dixon L, Brison DR. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod 2002; 17: 990-998. [DOI:10.1093/humrep/17.4.990]
4. Zini A, Meriano J, Kader K, Jarvi K, Laskin CA, Cadesky K. Potential adverse effect of sperm DNA damage on embryo quality after ICSI. Hum Reprod 2005; 20: 3476-3480. [DOI:10.1093/humrep/dei266]
5. Borini A, Tarozzi N, Bizzaro D, Bonu MA, Fava L, Flamigni C. Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. Hum Reprod 2006; 21: 2876-2881. [DOI:10.1093/humrep/del251]
6. Zini A, Kamal KM, Phang D. Free thiols in human spermatozoa: correlation with sperm DNA integrity. Urology 2001; 58: 80-84. [DOI:10.1016/S0090-4295(01)00997-9]
7. Carrell D T, Emery BR, Hammoud S. Altered protamine expression and diminished spermatogenesis: What is link? Hum Reprod Update 2007; 13: 313-327. [DOI:10.1093/humupd/dml057]
8. Tesarik J, Mendoza-Tesarik R, Mendoza C. Sperm nuclear DNA damage: update on the mechanism, diagnosis and treatment. Reprod Biomed Online 2006; 12: 715-721. [DOI:10.1016/S1472-6483(10)61083-8]
9. Duru NK, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 200l; 74: 1200-1207.
10. Oosterhuis GJ, Mulder AB, Kalsbeek-Batenburg E, Lambalk CB, Schoemaker J, Vermes I, Measuring apoptosis in human spermatozoa: a biological assay for semen quality? Fertil Steril 2000; 74: 245-250. [DOI:10.1016/S0015-0282(00)00623-3]
11. Shen HM, Dai J, Chia SE, Lim A, Ong CN. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Hum Reprod 2002; 17: 1266-1273. [DOI:10.1093/humrep/17.5.1266]
12. Moustafa MH, Rakesh K, Sharma RK,Mascha E, Abdel-Hafez MA, Thomas AJ Jr, et al. Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum Reprod 2004; 19: 129-138. [DOI:10.1093/humrep/deh024]
13. Rai R, Regan L. Recurrent miscarriage. Lancet 2006; 368: 601-611. [DOI:10.1016/S0140-6736(06)69204-0]
14. Andrew WH, Claire IA. Recurrent miscarriage. J Fam Plann Reprod Health Care 2005; 31: 103-107. [DOI:10.1783/1471189053629428]
15. Gil-Villa AM, Cardona-Maya W, Agarwal A, Sharma R, Cadavid A. Role of male factor in early recurrent embryo loss: do antioxidants have any effect? Fertil Steril 2009; 92: 565-571. [DOI:10.1016/j.fertnstert.2008.07.1715]
16. Sutovsky P, Schatten G. Paternal contributions to the mammalian zygote: fertilization after sperm-egg fusion. Int Rev Cytol 2000; 195: 1-65.
17. Talebi AR, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Dehghani Firoozabadi R, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia 2012; 1: 462-470. [DOI:10.1111/j.1439-0272.2011.01206.x]
18. World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge University Press, Cambridge; 1999.
19. Talebi AR, Khalili MA, Vahidi S, Ghasemzadeh J, Tabibnejad N. Sperm chromatin condensation, DNA integrity, and apoptosis in men with spinal cord injury. J Spinal Cord Med 2013; 2: 140-146. [DOI:10.1179/2045772312Y.0000000055]
20. Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 4: 245-251. [DOI:10.1111/j.1439-0272.2008.00852.x]
21. Kazerooni T, Asadi N, Jadid L,Kazerooni M, Ghanadi A, Ghaffarpasand F, et al. Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet 2009; 26: 591-596. [DOI:10.1007/s10815-009-9361-3]
22. Lundin K, Soderlund B, Hamberger L. The relationship between sperm morphology and rates of fertilization, pregnancy and spontaneous abortion in an in-vitro fertilization/ intracytoplasmic sperm injection programme. Hum Reprod 1997; 12: 2676-2681. [DOI:10.1093/humrep/12.12.2676]
23. Gupta S, Agarwal A, Banerjee J, Alvarez JG. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: a systematic review. Obstet Gynecol Surv 2007; 62: 335-347 [DOI:10.1097/01.ogx.0000261644.89300.df]
24. Bhattacharya SM. Association of various sperm parameters early pregnancy loss-which is most important? Int Urol Nephrol 2008; 2: 391-395. [DOI:10.1007/s11255-007-9282-y]
25. Lolis D, Georgiou I, Syrrou M, Zikopoulos K, Konstantelli M, Messinis I. Chromomycin A3-staining as an indicator of protamine deficiency and fertilization. Int J Androl 1996; 19: 23-27. [DOI:10.1111/j.1365-2605.1996.tb00429.x]
26. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9: 331-345. [DOI:10.1093/humupd/dmg027]
27. Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril 2004; 81: 1289-1295. [DOI:10.1016/j.fertnstert.2003.09.063]
28. Sakkas D, Seli E, Bizzaro D, Tarozzi N, Manicardi GC. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis. Reprod Biomed Online 2003; 7: 428-432. [DOI:10.1016/S1472-6483(10)61886-X]
29. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA dena-turation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674-677. [DOI:10.1016/S0015-0282(00)01796-9]
30. Braude P, Bolton V, Moore S. Human gene expression first occurs between the fourand eight-cell stages of preimplantation development. Nature 1988; 332: 459-461. [DOI:10.1038/332459a0]
31. Morris ID, Ilott S, Dixon L, Brison DR. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod 2002; 17: 990-998. [DOI:10.1093/humrep/17.4.990]
32. Tesarik J, Greco E, Mendoza C. Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation. Hum Reprod 2004; 19: 611-615. [DOI:10.1093/humrep/deh127]
33. Gil-Villa AM, Cardona-Maya W, Agarwal A, Sharma R, Cadavid A. Assessment of sperm factors possibly involved in early recurrent pregnancy loss. Fertil Steril 2009; 4: 1465-1472.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb