Volume 14, Issue 2 (2-2016)                   IJRM 2016, 14(2): 75-80 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimi Jashni H, Kargar Jahromi H, Ranjbary A G, Kargar Jahromi Z, Khabbaz Kherameh Z. Effects of aqueous extract from Asparagus officinalis L. roots on hypothalamic-pituitary-gonadal axis hormone levels and the number of ovarian follicles in adult rats. IJRM. 2016; 14 (2) :75-80
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-727-en.html
1- Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
2- Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran , hossein.kargarjahromy@yahoo.com
3- Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Abstract:   (96 Views)
Background: Asparagus is a plant with high nutritional, pharmaceutical, and industrial values. Objective: The present study aimed to evaluate the effect of aqueous extract of asparagus roots on the hypothalamic-pituitary-gonadal axis hormones and oogenesis in female rats. Materials and Methods: In this experimental study, 40 adult female Wistar rats were divided into five groups, which consist 8 rats. Groups included control, sham and three experimental groups receiving different doses (100, 200, 400 mg/kg/bw) of aqueous extract of asparagus roots. All dosages were administered orally for 28 days.  Blood samples were taken from rats to evaluate serum levels of Gonadotropin releasing hormone (GnRH), follicular stimulating hormone (FSH), Luteinal hormone (LH), estrogen, and progesterone hormones. The ovaries were removed, weighted, sectioned, and studied by light microscope. Results: Dose-dependent aqueous extract of asparagus roots significantly increased serum levels of GnRH, FSH, LH, estrogen, and progestin hormones compared to control and sham groups. Increase in number of ovarian follicles and corpus luteum in groups treated with asparagus root extract was also observed (p<0.05). Conclusion: Asparagus roots extract stimulates secretion of hypothalamic- pituitary- gonadal axis hormones. This also positively affects oogenesis in female rats.
Full-Text [PDF 221 kb]   (30 Downloads) |   |   Full-Text (HTML)  (12 Views)  
Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2017/10/4 | Published: 2017/10/4

1. Bousfield JR, Jia l, Ward DN. Gonadotropins: chemistry and biosynthesis. In: Physiology of reproduction. Neill JD (ed), Elsevier-Academic Press, 2006:1581-1634.
2. Constantin S. Physiology of the GnRH neuron: Studies from embryonic GnRH neurons. J Neuroendocrinol 2011; 23: 542-553. [DOI:10.1111/j.1365-2826.2011.02130.x]
3. Sarabi B, Hassandokht MR, Hassani ME, Ramak Masumi T. Evaluation of Morphological Characteristics of Iranian Edible Wild Asparagus (Asparagus officinalis L.). Iran J Horticultural Sci 2010; 41: 197-207.
4. Al-Snafi AE. The pharmacological importance of Asparagus officinalis: A review. J Pharm Biol 2015; 5: 93-98.
5. Lee EJ, Yoo KS, Patil BS. Development of a rapid HPLC-UV method for simultaneous quantification of protodioscin and rutin in white and green asparagus spears. J Food Sci 2010; 75: 703-709. [DOI:10.1111/j.1750-3841.2010.01824.x]
6. Visavadiya N, Narasimhacharya A. Asparagus Root Vegulates Cholesterol Metabolism and Improves Antioxidant Status in Hypercholesteremic rats. Evid Base Comp Altern Med 2009; 6: 219-226. [DOI:10.1093/ecam/nem091]
7. Negi JS, Singh P, Joshi GP, Rawat MS, Bisht VK. Chemical constituents of asparagus. Pharmacogn Rev 2010; 4: 215-220. [DOI:10.4103/0973-7847.70921]
8. Verma SP, Tripathi VC, Das P. Asparagus racemosus leaf extract inhibits growth of UOK 146 renal cell carcinoma cell line: Simultaneous Oncogenic PRCCTFE3 Fusion Transcript Inhibition and Apoptosis independent cell death. Asian Pac J Cancer Prev 2014; 15: 1937-1941. [DOI:10.7314/APJCP.2014.15.5.1937]
9. Chin CK, Garrison SA, Ho CT, Shao Y, Wang M, Simon J, et al. Functional elements from asparagus for human health. Acta Hort 2002; 589: 233-237. [DOI:10.17660/ActaHortic.2002.589.32]
10. Kim BY, Cui ZG, Lee SR, Kim SJ, Kang HK, Lee YK, et al. Effects of Asparagus officinalis extracts on liver cell toxicity and ethanol metabolism. J Food Sci 2009; 74: 204-208. [DOI:10.1111/j.1750-3841.2009.01263.x]
11. Mehrafarin A. A review on garden Asparagus (Asparagus officinalis L.) as a medicinal and multipurpose plant. J Med Plants 2011; 3: 1-13.
12. Zhang QH, Wu CF, Duan L, Yang JY. Protective effects of total saponins from stem and leaf of Panax ginseng against cyclophosphamide-induced genotoxicity and apoptosis in mouse bone marrow cells and peripheral. Food Chem Toxicol 2008; 46: 293-302. [DOI:10.1016/j.fct.2007.08.025]
13. Zhu X, Zhang W, Zhao J, Wang J, Qu W. Hypolipidaemic and hepatoprotective effects of ethanolic and aqueous extracts from Asparagus officinalis L. by-products in mice fed a high-fat diet. J Sci Food Agric 2010; 90: 1129-1135. [DOI:10.1002/jsfa.3923]
14. Chen EC, Samuels MH, Luther MF, King TS, Eddy CA, Siler-Khodr TM, et al. Cocaine impairs follicular phase pulsatile gonadotropin secretion in rhesus monkey. J Soc Gynecol Invest 1998; 5: 311-316. [DOI:10.1177/107155769800500606]
15. Nematollahi Mahani N, Amini S, Imami Meibodi MA, Nabipour F, Eftekhar Vaghefi H. Effects of morphine dependency on the ovarian folliculogenesis folloing superovulation in mice. J Kerman Univ Med Sci 2005; 12: 174-180.
16. Hewawasam RP, Jayatilaka KA, Pathirana C. Effect of Asparagus falcatus on acetaminophen toxicity in mice: a comparison of antioxidative effect with N-acetyl cysteine. J Diet Suppl 2008; 5: 1-19. [DOI:10.1080/19390210802328933]
17. Pampillo M, Scimonelli T, Bottin MC, Duvilanski BH, Rettori V, Seilicovich A, et al. The effect of D-aspartate on luteinizing hormone-releasing hormone, α-melanocyte-stimulating hormone, GABA and dopamine release. Neuroreport 2002; 13: 2341-2344. [DOI:10.1097/00001756-200212030-00034]
18. Sato Y, Tsukanmamoto T. Effect of nitric oxide stimulation on the brain. Drugs Today 2000; 36: 83-92. [DOI:10.1358/dot.2000.36.2-3.568781]
19. Pinilla L, González LC, Tena-Sempere M, Bellido C, Aguilar E. Effects of systemic blockade of nitric oxide synthases on pulsatile lh, prolactin, and GH secretion in adult male rats. Horm Res 2001; 55: 229-235. [DOI:10.1159/000050001]
20. Omer N, Rohilla A, Rohilla S, Kushnoor A. Nitric Oxide: Role in Human Biology. Int J Pharm Sci Drug Res 2012; 4: 105-109.
21. Shao Y, Poobrasert O, Kennelly EJ, Chin CK, Ho CT, Huang MT, et al. Steroidal saponins from Asparagus officinalis and their cytotoxic activity. Plant Med 1997; 63: 258-262. [DOI:10.1055/s-2006-957667]
22. Modaresi M, Mahdian B. The effect of hydroalcoholic extract of Trigonella foenum- Graceum L. on reproductive system in Balb/c. J Herbal Drugs 2012; 2: 261-267.
23. Mokhtari M, Shariati M, Ghahramani R. Effect of Trigonella foenum- Graceum L. seed extract on concentration of testestrone and spermatogenesis in rats. J Med Plants 2008; 7: 12-20.
24. Nandi PR, Mehta BK, Sengupta D. Changes in concentration of minerals in follicular fluid of growing follicules. Exp Anim Med Res 2012; 2: 166-169.
25. Azadi Kohnehshahri V, Moghaddam Gh, Pirany N,Daghigh Kia H. The relationship between some blood elements and metabolites with repeat breeding in dairy cows at Azarnegin dairy farm. Anim Sci Res 2013; 23: 101-112.
26. Quirk SM, Cowan RG, Harman RM, Hu CL, Porter DA. Ovarian follicular growth and atresia: The relationship between cell proliferation and survival. J Anim Sci 2004; 82: 40-52. [DOI:10.2527/2004.8213_supplE40x]

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author

© 2018 All Rights Reserved | International Journal of Reproductive Biomedicine

Designed & Developed by : Yektaweb