Volume 13, Issue 8 (9-2015)                   IJRM 2015, 13(8): 507-512 | Back to browse issues page

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Safian F, Khalili M A, Karimi-Zarchi M, Mohsenzadeh M, Ashourzadeh S, Omidi M. Developmental competence of immature oocytes aspirated from antral follicles in patients with gynecological diseases. IJRM. 2015; 13 (8) :507-512
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-673-en.html
1- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , drkarimi2001@yahoo.com
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4- Afzalipour Clinical Center for Infertility, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran
Abstract:   (1005 Views)
Background: In vitro maturation (IVM) of immature oocytes collected from ovary has been proposed for fertility preservation. In addition, quality of oocytes post IVM is one of the factors determining its developmental competence. By using the non-invasive Polscope system, both meiotic spindle (MS) and zona pellucida (ZP) can be assessed in living oocytes.
Objective: The aim was to investigate the developmental potential of immature oocytes retrieved from ovarian tissue after IVM, as a method for fertility preservation, in patients with gynecological diseases.
Materials and Methods: The ovarian cortex from 26 patients with malignant and benign diseases (21-45 years old), were obtained directly from collaborating hospitals, and transported to the IVF center on ice. In total 61 immature oocytes were aspirated, of which 18 (29.5%) were degenerated and discarded. The remaining 43 (70.5%) healthy oocytes were cultured in IVM culture media for 48 hr. The rate of maturity was assessed, and the ZP birefringence and MS were imaged with Polscope technology.
Results: Overall 43 immature oocytes underwent IVM technology, of which 30.2% reached viable metaphase II (MII) oocytes. The ovarian tissues of 9 (34.6%) women were lacking oocytes at any stage. During polarized light microscopy examination, MS could be visualized only in one of the MII oocytes, but high ZP birefringence’s were observed in the majority of the oocytes post IVM (61.5%).
Conclusion: Oocytes maturation post IVM from unstimulated ovaries showed a good developmental competence in gynecologic patients. Further studies should be performed to advance the oocyte maturation program, such as co-culture system, for fertility preservation.
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Type of Study: Original Article |

1. Huang JY, Tulandi T, Holzer H, Tan SL, Chian R-C. Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation. Fertil Steril 2008; 89: 567-572. [DOI:10.1016/j.fertnstert.2007.03.090]
2. Kim CH, Jeon GH. Fertility preservation in female cancer patients. ISRN obstet gynecol 2012; 2012: 807302.
3. Chung K, Donnez J, Ginsburg E, Meirow D. Emergency IVF versus ovarian tissue cryopreservation: decision making in fertility preservation for female cancer patients. Fertil steril 2013; 99: 1534-1542. [DOI:10.1016/j.fertnstert.2012.11.057]
4. Cromi A, Bogani G, Uccella S, Casarin J, Serati M, Ghezzi F. Laparoscopic fertility-sparing surgery for early stage ovarian cancer: a single-centre case series and systematic literature review. J Ovarian Res 2014; 7: 59. [DOI:10.1186/1757-2215-7-59]
5. Cakmak H, Rosen MP. Ovarian stimulation in cancer patients. Fertil steril 2013; 99: 1476-1484. [DOI:10.1016/j.fertnstert.2013.03.029]
6. Fatemi HM, Kyrou D, Al-Azemi M, Stoop D, De Sutter P, Bourgain C, et al. Ex-vivo oocyte retrieval for fertility preservation. Fertil steril 2011; 95: 1787. [DOI:10.1016/j.fertnstert.2010.11.023]
7. Chian R-C, Uzelac PS, Nargund G. In vitro maturation of human immature oocytes for fertility preservation. Fertil steril 2013; 99: 1173-1181. [DOI:10.1016/j.fertnstert.2013.01.141]
8. Huang J, Tulandi T, Holzer H, Lau N, Macdonald S, Tan S, et al. Cryopreservation of ovarian tissue and in vitro matured oocytes in a female with mosaic Turner syndrome: case report. Hum Reprod 2008; 23: 336-339. [DOI:10.1093/humrep/dem307]
9. Wilken-Jensen HN, Kristensen SG, Jeppesen JV, Yding Andersen C. Developmental competence of oocytes isolated from surplus medulla tissue in connection with cryopreservation of ovarian tissue for fertility preservation. Acta Obstet Gynecol Scand 2014; 93: 32-37. [DOI:10.1111/aogs.12264]
10. Ata B, Chian R-C, Tan SL. Cryopreservation of oocytes and embryos for fertility preservation for female cancer patients. Best Pract Res Clin Obstet Gynaecol 2010; 24: 101-112. [DOI:10.1016/j.bpobgyn.2009.11.007]
11. Fadini R, Dal Canto M, Mignini Renzini M, Milani R, Fruscio R, Cantù MG, et al. Embryo transfer following in vitro maturation and cryopreservation of oocytes recovered from antral follicles during conservative surgery for ovarian cancer. J Assist Reprod Genet 2012: 29: 779-781. [DOI:10.1007/s10815-012-9768-0]
12. Revel A, Safran A, Benshushan A, Shushan A, Laufer N, Simon A. In vitro maturation and fertilization of oocytes from an intact ovary of a surgically treated patient with endometrial carcinoma: case report. Hum Reprod 2004; 19: 1608-1611. [DOI:10.1093/humrep/deh241]
13. Imesch P, Scheiner D, Xie M, Fink D, Macas E, Dubey R, et al. Developmental potential of human oocytes matured in vitro followed by vitrification and activation. J Ovarian Res 2013; 6: 30. [DOI:10.1186/1757-2215-6-30]
14. Khalili MA, Nottola SA, Shahedi A, Macchiarelli G. Contribution of human oocyte architecture to success of in vitro maturation technology. Iran J Reprod Med 2013; 11: 1-10.
15. Omidi M, Khalili MA, Ashourzadeh S, Rahimipour M. Zona pellucida birefringence and meiotic spindle visualisation of human oocytes are not influenced by IVM technology. Reprod Fertil Dev 2014; 26: 407-413. [DOI:10.1071/RD13001]
16. Molinari E, Evangelista F, Racca C, Cagnazzo C, Revelli A. Polarized light microscopy-detectable structures of human oocytes and embryos are related to the likelihood of conception in IVF. J Assisted Reprod Genet 2012; 29: 1117-1122. [DOI:10.1007/s10815-012-9840-9]
17. Mohsenzadeh M, Khalili MA, Nazari S, Jahromi VH, Agharahimi A, Halvaei I. Effect of vitrification on morphology and in-vitro maturation outcome of human immature oocytes. Ital J Anat Embryol 2012; 117: 190-198.
18. Yazdanpanah F, Khalili MA, Eftekhar M, Karimi H. The effect of vitrification on maturation and viability capacities of immature human oocytes. Arch Gynecol Obstet 2013; 288: 439-444. [DOI:10.1007/s00404-013-2777-0]
19. Khalili MA, Mojibian M, Sultan A-M. Role of oocyte morphology on fertilization and embryo formation in assisted reproductive techniques. Middle East Fertility Society Journal 2005; 10: 72-77.
20. Serhal P, Ranieri D, Kinis A, Marchant S, Davies M, Khadum I. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum Reprod 1997; 12: 1267-1270. [DOI:10.1093/humrep/12.6.1267]
21. Omidi M, Khalili MA, Nahangi H, Ashourzadeh S, Rahimipour M. Does women's age influence zona pellucida birefringence of metaphase ΙΙ oocytes in in-vitro maturation program? Iran J Reprod Med 2013; 11: 823-828.
22. Fasano G, Moffa F, Dechène J, Englert Y, Demeestere I. Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation. Reprod Biol Endocrinol 2011; 9: 150.
23. Escribá M-J, Grau N, Escrich L, Novella-Maestre E, Sánchez-Serrano M. Spontaneous in vitro maturation of oocytes prior to ovarian tissue cryopreservation in natural cycles of oncologic patients. J Assist Reprod Genet 2012; 29: 1261-1265.
24. Cha K-Y, Chian R-C. Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update 1998; 4: 103-120.
25. Xia P. Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod 1997; 12: 1750-1755.
26. Nazari S, Khalili MA, Esmaielzadeh F, Mohsenzadeh M. Maturation capacity, morphology and morphometric assessment of human immature oocytes after vitrification and in-vitro maturation. Iran J Reprod Med 2011; 9: 209-216.
27. Rienzi L, Ubaldi FM, Iacobelli M, Minasi MG, Romano S, Ferrero S, et al. Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil Steril 2008; 90: 1692-1700.
28. Halvaei I, Khalili MA, Razi MH, Nottola SA. The effect of immature oocytes quantity on the rates of oocytes maturity and morphology, fertilization, and embryo development in ICSI cycles. J Assist Reprod Genet 2012; 29: 803-810.
29. Miao Y, Ma S, Liu X, Miao D, Chang Z, Luo M, et al. Fate of the first polar bodies in mouse oocytes. Mol Reprod Dev 2004; 69: 66-76.
30. Braga DP, Figueira RdeS, Rodrigues D, Madaschi C, Pasqualotto FF, Iaconelli Jr A, et al. Prognostic value of meiotic spindle imaging on fertilization rate and embryo development in in vitro-matured human oocytes. Fertil Steril 2008; 90: 429-433.
31. Raju G, Prakash G, Krishna KM, Madan K. Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod Biomed Online 2007; 14: 166-174.
32. Lasienė K, Vitkus A, Valančiūtė A, Lasys V. Morphological criteria of oocyte quality. Medicina Kaunas 2009; 45: 509-515.
33. Braga DPdAF, Figueira RdCS, Queiroz P, Madaschi C, Iaconelli A, Borges E. Zona pellucida birefringence in in vivo and in vitro matured oocytes. Fertil Steril 2010; 94: 2050-2053.
34. Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Silva LF, Cavagna M, et al. Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles. Reprod Biol Endocrinol 2011; 9: 53.  

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