Volume 13, Issue 12 (1-2015)                   IJRM 2015, 13(12): 765-770 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shekarforoush S, Ebrahimi Z, Hoseini M. Sodium metabisulfite-induced changes on testes, spermatogenesis and epididymal morphometric values in adult rats. IJRM. 2015; 13 (12) :765-770
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-615-en.html
1- Department of Physiology, Islamic Azad University, Arsanjan Branch, Fars, Iran , shek@iaua.ac.ir
2- Department of Physiology, Islamic Azad University, Arsanjan Branch, Fars, Iran
Abstract:   (972 Views)
Background: Sulphites are widely used as a preservative and antioxidant additives in the food and pharmaceutical industries. Many types of biological and toxicological effects of sulphites in multiple organs of mammals have been shown in previous studies. Objective: The aim of this study was to investigate the effects of sodium metabisulfite (SMB) on testicular function and morphometric values of epididymis in adult male Wistar rats. Materials and Methods: A total of 32 rats were randomly divided into four groups. The experimental groups received SMB at doses of 10 mg/kg (S10), 100mg/kg (S100), and 260 mg/kg (S260) while an equal volume of normal saline was administered to the control group via gavage. The rats were anaesthetized after 28 days and the left testis with the head of epididimis was excised following abdominal incision for histological observation using hematoxylin and eosin staining. Serum samples were collected for assay of testosterone level. The initial epididymis was analyzed for motility, morphology, and the number of sperms. Result: The results of this study showed that normal morphology, count, and motility of sperms and testosterone level were decreased in the SMB treated groups. In comparison with the control group, SMB resulted in a lower total number of spermatogonia, primary spermatocyte, spermatids, and Leydig cells. Conclusion: It is suggested that SMB decreases the sperm production and has the potential to affect the fertility adversely in male rats.
Full-Text [PDF 395 kb]   (170 Downloads) |   |   Full-Text (HTML)  (11 Views)  
Type of Study: Original Article |

References
1. Adebayo OL, Adenuga GA. Oxidative damage on the testes of adult rats by sodium metabisulfite (MBS). Int J Biolog Chem Sci 2012; 6: 738-744. [DOI:10.4314/ijbcs.v6i2.17]
2. Ozturk OH, Oktar S, Aydin M, Kucukatay V. Effect of sulfite on antioxidant enzymes and lipid peroxidation in normal and sulfite oxidase-deficient rat erythrocytes. J Physiol Biochem 2010; 66: 205-212. [DOI:10.1007/s13105-010-0025-7]
3. Nair B, Elmore AR. Final report on the safety assessment of sodium sulfite, potassium sulfite, ammonium sulfite, sodium bisulfite, ammonium bisulfite, sodium metabisulfite and potassium metabisulfite. Int J Toxicol 2003; 22: 63-88. [DOI:10.1080/10915810305077X]
4. Meng Z, Bai W. Oxidation damage of sulfur dioxide on testicles of mice. Environ Res 2004; 96: 298-304. [DOI:10.1016/j.envres.2004.04.008]
5. Baud O, Laudenbach V, Evrard P, Gressens P. Neurotoxic effects of fluorinated glucocorticoid preparations on the developing mouse brain: role of preservatives. Pediatr Res 2001; 50: 706-711. [DOI:10.1203/00006450-200112000-00013]
6. Ozturk N, Yargicoglu P, Derin N, Akpinar D, Agar A, Aslan M. Dose-dependent effect of nutritional sulfite intake on visual evoked potentials and lipid peroxidation. Neurotoxicol Teratol 2011; 33: 244-254. [DOI:10.1016/j.ntt.2010.09.002]
7. Noorafshan A, Asadi-Golshan R, Karbalay-Doust S, Abdollahifar MA, Rashidiani-Rashidabadi A. Curcumin, the main part of turmeric, prevents learning and memory changes induced by sodium metabisulfite, a preservative agent, in rats. Exp Neurobiol 2013; 22: 23-30. [DOI:10.5607/en.2013.22.1.23]
8. Nasiraei-Moghadam SN, Parivar K, Ahmadiani A, Movahhedin M, Vaez Mahdavi MR. Protective Effect of Melatonin against Inequality-Induced Damages on Testicular Tissue and Sperm Parameters. Int J Fertil Steril 2014; 7: 313-322.
9. Adebayo AO, Olurode SA. The morphology and morphometry of the epididymis in the greater cane rat (Thryonomys swinderianus Temmincks). Folia Morphol 2010; 69: 246-252.
10. Ahangarpour A, Oroojan AA, Radan M. Effect of aqueous and hydro-alcoholic extracts of lettuce (Lactuca sativa) seed on testosterone level and spermatogenesis in NMRI mice. Iran J Reprod Med 2014; 12: 65-72.
11. Derin AT, Kose O, Derin N, Savcioglu F, Hacioglu G, Ozcaglar HU, et al. Auditory brainstem response disturbances in rats induced by sodium metabisulfite ingestion. Mediterr J Otol 2008; 4: 77-85.
12. Elmas O, Aslan M, Caglar S, Derin N, Agar A, Aliciguzel Y, et al. The prooxidant effect of sodium metabisulfite in rat liver and kidney. Regul Toxicol Pharmacol 2005; 42: 77-82. [DOI:10.1016/j.yrtph.2005.01.010]
13. Ercan S, Ozturk N, Celik-Ozenci C, Gungor NE, Yargicoglu P. Sodium metabisulfite induces lipid peroxidation and apoptosis in rat gastric tissue. Toxicol Ind Health 2010; 26: 425-431. [DOI:10.1177/0748233710369665]
14. Shi X, Mao Y. 8-Hydroxy-2'-deoxyguanosine formation and DNA damage induced by sulfur trioxide anion radicals. Biochem Biophys Res Commun 1994; 30: 141-147. [DOI:10.1006/bbrc.1994.2641]
15. Izgut-Uysal VN, Kucukatay V, Bulbul M, Tan R, Yargicoglu P, Agar A. Effect of sulfite on macrophage functions of normal and sulfite oxidase-deficient rats. Food Chem Toxicol 2005; 43: 599-605. [DOI:10.1016/j.fct.2005.01.003]
16. Noorafshan A, Rashidiani-Rashidabadi A, Karbalay-Doust S, Poostpasand A, Abdollahifar MA, Asadi-Golshan R. Curcumin can prevent the changes in cerebellar structure and function induced by sodium metabisulfite in rat. Exp Neurobiol 2013; 22: 258-267. [DOI:10.5607/en.2013.22.4.258]
17. Selevan SG, Borkovec L, Slott VL, Zudova Z, Rubes J, Evenson DP, et al. Semen quality and reproductive health of young Czech men exposed to seasonal air pollution. Environ Health Perspect 2000; 108: 887-894. [DOI:10.1289/ehp.00108887]
18. Zhang J, Liang C, Ma J, Zhou B, Wang J. Changes in testis protein and metabolic enzyme activities in rats induced by sodium fluoride and sulfur dioxide. Fluoride 2006; 39: 179-184.
19. Sofikitis N, Giotitsas N, Tsounapi P, Baltogiannis D, Giannakis D, Pardalidis N. Hormonal regulation of spermatogenesis and spermiogenesis. J Steroid Biochem Mol Biol 2008; 109: 323-330. [DOI:10.1016/j.jsbmb.2008.03.004]
20. Smith LB, Walker WH. The regulation of spermatogenesis by androgens. Semin Cell Dev Biol 2014; 30: 2-13. [DOI:10.1016/j.semcdb.2014.02.012]
21. Suresh S, Shanthi Santhosh Kumari S, Preethi U, Venkatalakshmi N, Karthik Ganesh M, Ganesh L, et al. Long-Term Hyperglycemic effect on rat Epididymis and Sperm. Int J Anat Sci 2011; 2: 11-17.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb