Volume 12, Issue 7 (8-2014)                   IJRM 2014, 12(7): 459-0 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Askari Jahromi M, Movahedin M, Mazaheri Z, Amanlu M, Mowla S J, Batooli H. Evaluating the effects of Escanbil (Calligonum) extract on the expression level of Catsper gene variants and sperm motility in aging male mice. IJRM. 2014; 12 (7) :459-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-567-en.html
1- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , movahed.m@modares.ac.ir
3- Department of Medicinal Chemistry, Faculty of Pharmacy, Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Genetic, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
5- Isfahan Research Center of Agriculture and Natural Resources, Kashan Botanical Garden, Kashan, Iran
Abstract:   (1096 Views)
Background: Catsper proteins are responsible for entering Ca2+ to the cell and play an important role in sperm motility and male fertility. Antioxidants are vital for sperm motility too. Escanbil (Calligonum) extract possess some of the important antioxidant like Catechin and Quercetin.
Objective: Here we investigated the effects of Escanbil (Calligonum) extract on the sperm parameters and the expressing of Catsper gene in aging male mice.
Materials and Methods: In this animal study, firstly, dose response was performed by using these three doses of Escanbil (Calligonum) (10, 30 and 50 mg/kg). 5 mice in each group were considered and Intra Peritoneal injection was done for 5 weeks. the sperm parameters analyzed and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL )staining was done. 30 mg/kg dose was considered as optimum dose. Secondly: fifteen aging male mice (11-13 months) were divided into three groups: control, sham and experiment. The experiments were injected Intra peritonealy with Escanbil (Calligonum) extract (30mg/kg) weekly for up to 5 weeks. The sham group was injected Intra Peritoneal (DMSO). Sperm parameters were analyzed. Expression of Catsper genes was analyzed by Real time PCR.
Results: Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05). Our data showed that there was a statistical significance difference between the expressions of Catsper 2, 4 in aging experiment group comparison with aging control group (p<0.05).
Conclusion: We investigated that the Escanbil (Calligonum) extract (30 mg/kg) can improve sperm parameters and change the expression of Catsper genes in aging male mice. This herbal extract can be used as an antioxidant component for clinical usages.
 
Full-Text [PDF 284 kb]   (121 Downloads) |   |   Full-Text (HTML)  (71 Views)  
Type of Study: Original Article |

References
1. Tanemura K, Kurohmaru M, Kuramoto K, Hayashi Y. Age related morphological changes in the testis of the BDFI mouse. J Vet Med Sci 1993; 133: 2773-2781.
2. Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas AJ Jr. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril 2004; 81: 349-354. [DOI:10.1016/j.fertnstert.2003.06.026]
3. Weyrobek AJ, Eskenazi B, Young S, Arnheim N, Boege IT, Jabs EW, et al. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutation, and aneuploidies in sperm. Proc Natl Acad Sci U S A 2006; 103: 9601-9606. [DOI:10.1073/pnas.0506468103]
4. Agarwal A, Gupta S, Sikka S. The role of free radicals and antioxidants in reproduction. Curr Opin Obstet Gynecol 2006; 18: 325-332. [DOI:10.1097/01.gco.0000193003.58158.4e]
5. Amini Rad O, Khalili MA, Soltani Gord Faramarzi HR. Influence of pomegranate juice on sperm parameters and fertility in mice. Hormozgan Med J 2009; 13: 182-188.
6. Mohamadi S, Movahedin M, Mowla SJ. The effects of selenium on sperm parameters and the testis tissue in aging and young male mice. J Reprod Infertil 2008; 36: 229-237.
7. Aviram M, Dornfeld L, Kaplan M, Coleman R, Gaitini D, Nitecki S. Pomegranate juice flavonoids inhibit low-density lipoprotein oxidation and cardiovascular diseases: studies in atherosclerotic mice and in humans. Drugs Exp Clin Res 2002; 28: 49-62.
8. Badria FA, Ameen M, Akl MR. Evaluation of cytotoxic compounds from calligonum comosum L. growing in Egypt. Z Naturforsch C 2007; 62: 656-660. [DOI:10.1515/znc-2007-9-1005]
9. Boonsorn T, Kongbuntad W, Narrkong N, Aengwanich X. Effects of catechin addition to extender on sperm quality and lipid proxidation in boar semen. Am Eurasian 2010; 7: 283-288.
10. Med JTE. Antioxidative action of Flavonoids, Quercetin and Catechin mediated by the Activation of Glutathione Peroxidase. Tokai J Exp Clin Med 1999; 24: 1-11.
11. Bedu-Addo K, Barratt CL, Kirkman-Brown JC, Publicover SJ. Patterns of [Ca2+] (i) mobilization and cell response in human spermatozoa exposed to progesterone. Dev Biol 2007; 302: 324-332. [DOI:10.1016/j.ydbio.2006.09.040]
12. Olson SD, Fauci LJ, Suarez S. A review of mathematical modeling of calcium signaling during sperm hyperactivation. Mol Hum Reprod 2011; 17: 500-510. [DOI:10.1093/molehr/gar040]
13. Ren D, Xia J, Reigada D, Mitchell CH. Catsper channel-mediated Ca2+ entry into mouse sperm triggers a tail-to-head propagation. Biol Reprod 2007; 77: 551-559. [DOI:10.1095/biolreprod.107.061358]
14. Carlson AE, Westenbroek RE, Quill T, Ren D, Clapham DE, Hille B, et al. CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm. Proc Natl Acad Sci USA 2003; 100: 14864-14868. [DOI:10.1073/pnas.2536658100]
15. Garbers DL. Hyperactivated sperm motility driven by Catsper2 is required for fertilization. Proc Natl Acad Sci USA 2003; 100: 14869-14874. [DOI:10.1073/pnas.2136654100]
16. Jin J, Odoherty AM, Wang Sh, Zheng H, Sandres KM. Catsper 3 and Catsper 4 encode Two cation channel-like proteins Exclusively in the testis. Biol Reprod 2005; 105: 454-468.
17. Wang H, Liu J, Cho KH, Ren D. A novel, single, transmembrane CATSPERG is associated with CATSPER1 channel protein. Biol Reprod 2009; 81: 539-544. [DOI:10.1095/biolreprod.109.077107]
18. Sikka SC. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Front Biosci 1996; 1: 78-86. [DOI:10.2741/A146]
19. Twigg J, Fulton N, Gomez E, Irvine DS, Aitken RJ. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. J Hum Reprod 1998; 13: 1429-1436. [DOI:10.1093/humrep/13.6.1429]
20. Naseri M, Heydari nasrabadi M, Khodarahmi P, Ahmadi F, Mojibi P, Abotalebei H. Study of the Effect of Fumaria parviflora Alcoholic Extract on Spermatogenesis in Male Rats. New Cell Mol Biotech J 2011; 1: 61-65.
21. Hosseini A, Zare S,Ghaderi pakdel F, Ahmadi A. American Ginseng effects on damage induced by Cyclophosphamide in the nuclei of rat sperm. J Reprod Infertil 2010; 45: 211-226.
22. Kenji S, Kou S. Oxidative stress induced by doxorubicin and protective effects of Green tea extract in conjunction with higher telomeras activity in mice. J Assist Reprod Genet 2010; 27: 501-508. [DOI:10.1007/s10815-010-9438-z]
23. Verma A, Kanwar KC. Human sperm motility and lipid peroxidation in different ascorbic acid concentrations an in vitro analysis. Andrologia 1998; 30: 325-329. [DOI:10.1111/j.1439-0272.1998.tb01178.x]
24. Arabi M, Sanyal SN, Kanwar U, Anand RJK. The Effect of antioxidants on nicotine and caffeine induced changes in human sperm. (eds. De Vriese SR, Christophe AB.). USA, AOCS Press; 2003: 250-267.
25. Kanakis CD, Tarantilis PA, Tajmir-Riahi HA, Polissiou MG. Crocetin, dimethylcrocetin and safranal bind human serum albumin: stability and antioxidative properties. J Agric Food Chem 2007; 55: 970-977. [DOI:10.1021/jf062638l]
26. Kowalski J, Samojedny A, Paul M, Pietsz G, Wilczok T. Effect of kaempferol on the production and gene expression of monocyte chemoattractant protein-1 in J774.2 macrophages. Pharmacol Rep 2005; 57: 107-112.
27. Cao L, Leers-Sucheta S, Azhar S. Aging alters the functional expression of enzymatic and non-enzymatic anti-oxidant defense systems in testicularrat Leydig cells. J Steroid Biochem Mol Biol 2004; 88: 61-67. [DOI:10.1016/j.jsbmb.2003.10.007]
28. Nikpoor P, Mowla SJ, Movahedin M, Ziaee SA. Catsper gene expression in subfertilemen with deficient sperm motility. Yakhteh 2004; 9: 1-9.
29. Gil-Guzman E, Ollero M, Lopez MC, Sharma RK, Alvarez JG, Thomas AJ Jr. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 2001; 16: 1922-1930. [DOI:10.1093/humrep/16.9.1922]
30. Kamkar A, Asadi, F, JebeliJavan A, Jamshidi R. Antioxidant capacity of essential oil and extract of Iranian Mentha spicat. J Vet Med Laboratory 2009; 69-77.
31. Liu XM, Zakaria MNM, Islam MW, Radhakrishnan R, Ismail A, Chen HB, et al. Anti-inflammatory and anti-ulcer activity of Calligonum comosum in rats. Fitoterapia P 2001; 487-491.
32. Garolla A, Maiorino M, Roverato A, Roveri A, Ursini F, Foresta C. Oral carnitine supplementation increases sperm motility in asthenozoospermic men with normal sperm phospholipid hydroperoxide glutathione peroxidase levels. For Steril 2005; 83: 355-361. [DOI:10.1016/j.fertnstert.2004.10.010]
33. Olugbenga OM, Gbadebo Olukole S, Tolulope Adeoye A, Deborah Adejoke A. Semen characteristics and sperm morphological studies of the West African Dwarf Buck treated with Aloe vera gel extract. Iran J Reprod Med 2011; 9: 83- 88.
34. Kobayashi H, Gil-Guzman E, Mahran AM, 59TRakesh59T, 59TNelson DR59T, 59TThomas AJ Jr59T, 59Tet59T al. Quality control of reactive oxygen species measurement by Iuminal-dependent chemiluminescence assay. J Androl 2001; 22: 568-574.
35. Mohammadi S, Movahedin M, Mowla SJ. Up-regulation of CatSper genes family by selenium. Repod Biol 2009; 7: 126. [DOI:10.1186/1477-7827-7-126]
36. Mohammadi S, Jalali M, Nikravesh MR, Gholamin M, Fazel A, Ebrahimzadeh A, Sankian M. Effects of L-carnitine treatment on expression of CatSper proteins in the aging mouse model. Eur J Exp Biol 2013; 3: 731-735.
37. Mohammadi S, Jalali M, Nikravesh MR, Fazel A, Ebrahimzadeh A, Gholamin M, et al. Effects of Vitamin-E treatment on CatSper genes expression and sperm quality in testis of aging mice. Iran J Reprod Med 2013; 11: 989-998.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb