Volume 12, Issue 1 (2-2014)                   IJRM 2014, 12(1): 57-0 | Back to browse issues page

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Hatami S, Zavareh S, Salehnia M, Lashkarbolouki T, Ghorbanian M T, Karimi I. The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries. IJRM. 2014; 12 (1) :57-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-474-en.html
1- School of Biology, Damghan University, Damghan, Iran
2- Department of Anatomy, Tarbiat Modares University, Tehran, Iran
3- Laboratory of Molecular and Cellular Biology, Department of Basic Veterinary Sciences, School of Veterinary Medicine, Razi University, Kermanshah, Iran
Abstract:   (1323 Views)
Background: Cryopreservation of ovarian tissues and pre-antral follicles is a promising prospect for preservation of women fertility.
Objective: The aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ALA).
Materials and Methods: Pre-antral follicles derived from fresh, vitrified-warmed ovarian tissues and vitrified–warmed pre-antral follicles were cultured individually with or without ALA, followed by adding hCG to induce ovulation. The follicle growth, oocyte maturation, and embryo development were assessed.
Results: The diameter and development of follicles, oocyte maturation and embryo development rates were significantly higher in ALA supplemented groups compared to the respective ALA-free conditions groups. Aforementioned parameters were significantly higher in vitrified-warmed follicles in comparison to follicles derived from vitrified-warmed ovaries.
Conclusion: These findings support a superior performance of pre-antral follicles when vitrified rather than when isolated from vitrified ovaries with regard to increasing the rates of developmental parameters. Moreover, ALA improves the in vitro maturation of pre-antral follicles in vitrified and non-vitrified samples. 
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Type of Study: Original Article |

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