Volume 11, Issue 7 (10-2013)                   IJRM 2013, 11(7): 545-0 | Back to browse issues page

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Kashyap B, Srivastava N, Kaur I R, Jhamb R, Singh D K. Diagnostic dilemma in female genital tuberculosis- staining techniques revisited. IJRM. 2013; 11 (7) :545-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-443-en.html
1- Department of Microbiology, University College of Medical Sciences, Guru Teg Bahadur Hospital, New Delhi, India, India , dr_bineetakashyap@yahoo.co.in
2- Department of Microbiology, University College of Medical Sciences, Guru Teg Bahadur Hospital, New Delhi, India, India
3- Department of Medicine, University College of Medical Sciences, Guru Teg Bahadur Hospital, New Delhi, India, India
4- Department of Pathology, University College of Medical Sciences, Guru Teg Bahadur Hospital, New Delhi, India, India
Abstract:   (85 Views)
Background: Tuberculosis (TB) is an increasing public health concern worldwide. On a global scale it has a devastating impact in developing nations. Genital TB, an extrapulmonary form, is not uncommon particularly in areas where pulmonary TB is prevalent. Genital TB may be asymptomatic or may even masquerade as other gynaecological conditions; hence, diagnosis requires a high degree of suspicion and the use of appropriate investigations.
Objective: This study attempted to identify endometrial TB in endometrial biopsies taken from women evaluated for infertility by comparison of various staining techniques.
Materials and Methods: A comparative cross sectional study was conducted from February 2011 to April 2011 in Guru Teg Bahadur Hospital, New Delhi. Endometrial biopsy specimens from 55 endometrial TB suspects were stained for acid fast bacilli by Ziehl Neelson staining and Gabbet staining. The biopsy samples were also subjected to Auramine Phenol fluroscent staining and H and E staining. Culture on Lowenstein Jensen medium was taken as the gold standard.
Results: Three samples were culture positive giving positivity rate of 5.4%. Considering culture as the gold standard the senstivities of ZN, Gabbet, fluorescent and H and E staining were 33, 33, 66, and 66% respectively while their specificities were 100, 100, 98, and100% respectively.
Conclusion: Combination of fluorescent staining techniques along with one of the acid fast staining techniques or histopathology achieves sufficient sensitivity and specificity for the diagnosis of female genital tuberculosis. There is an urgent need for developing definitive diagnostic methods to make a conclusive diagnosis of genital TB.
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Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2018/03/4 | Published: 2018/03/4

References
1. Kashyap B, Srivastava N, Kaur IR, Singh DK. Diagnostic dilemma in female genital tuberculosis- staining techniques revisited. DOTS DELHI NEWSLETTER Volume III/Issue III/ New Delhi 2011.
2. Rana T, Singh UB, Kulshrestha V, Kaushik A, Porwal C, Agarwal N, et al. Utility of reverse transcriptase PCR and DNA-PCR in the diagnosis of female genital tuberculosis. J Med Microbiol 2011; 60: 486-491. [DOI:10.1099/jmm.0.025080-0]
3. Roy A, Mukherjee S, Bhattacharya S, Adhya S, Chakraborty P. Tuberculous endometritis in hills of Darjeeling: a clinicopathological and bacteriological study. Indian J Pathol Microbiol 1993; 36: 361-369.
4. Punnonen R, Kiilholma P, Meurman L Female genital tuberculosis and consequent Infertility. Int J fertile 1983; 28: 235-238.
5. Nogales-Ortiz F, Ildefonso T, Nogales FF. The pathology of genital tuberculosis. Obstet Gynecol 1979; 53: 422-428.
6. Gupta N, Sharma JB, Mittal S, Singh N, Misra R, Kukreja M. Genital tuberculosis in Indian infertility patients. Int J Gynaecol Obstet 2007; 97:135-138. [DOI:10.1016/j.ijgo.2006.12.018]
7. Saraswat P, Swarankar ML, Bhandari A, Soni RR. Detection of active female genital tuberculosis by molecular method. Int J Pharma Bio Sci 2010; 1: B328-B334.
8. Padubidri V, Daftary SN. Tuberculosis of the Genital Tract. In: Howkins and Bourne, Shaw's. Textbook of Gynaecology. New Delhi: Churchill Livingstone; 1994: 155-164.
9. Tripathy SN, Tripathy SN. Genital involvement in pulmonary tuberculosis. Ind J Tub 1991; 38: 191-196.
10. Tripathy SN, Tripathy SN. Infertility and pregnancy outcome in female genital tuberculosis. Int J Gynecol Obstet 2002; 76: 159-163. [DOI:10.1016/S0020-7292(01)00525-2]
11. Zumla A, James G. Granulomatous infections: etiology and classification. Clin Infect Dis 1996; 23: 146-158. [DOI:10.1093/clinids/23.1.146]
12. Gokhale S, Qadir S, Nagra JS, Chakraborty AK. Efficiency of cold staining method of AFB in sputum - a comparison with Ziehl Neelsen Method under field condition. Ind J Tub 1990; 37: 135-137.
13. Mondal SK, Dutta TK. A ten year clinicopathological study of female genital tuberculosis and impact on fertility. J Nepal Med Assoc 2009; 48: 52-57.
14. Chowdhury RG, Paine SK, Bhattacharjee B, Chatterjee S. Infestation of Endometrium by Mycobacterium Tuberculosis Bacilli-Cause of Reproductive Failure. Al Ameen J Med Sci 2010; 3: 322-331.
15. Mohammad O, Almoujahed, Laurence E, Briski, Michael Prysak, Leonard B Johnson, Riad Khatib. Uterine Granulomas-Clinical and Pathologic Features. Am J Clin Pathol 2002; 117: 771-775. [DOI:10.1309/QFKL-WRWB-KYFE-KX2E]
16. Muechler M. Post-menopausal endometrial tuberculosis. Obstet Gynecol 1971; 38: 768-770.
17. Rozati R, Roopa S, Naga Rajeshwari Ch. Evaluation of women with infertility and genital tuberculosis. J Obstet Gynecol India 2006; 56: 423-426.

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