Volume 11, Issue 5 (7-2013)                   IJRM 2013, 11(5): 431-0 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Soleimanian S, Kalantar S M, Sheikhha M H, Zaimy M A, Rasti A, Fazli H. Association between Y-chromosome AZFc region micro-deletions with recurrent miscarriage. IJRM. 2013; 11 (5) :431-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-419-en.html
1- Molecular Medicine Research Center, Hormozgan Univercity of Medical Sciences, Bandarabbas, Iran
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , zaimy65@gmail.com
4- Department of Microbiology, Hamedan University of Medical Sciences, Hamedan, Iran
Abstract:   (583 Views)
Background: In human, about 25% of implanted embryos are losing 1-2 week following attachment to the uterus. A subset of this population will have three or more consecutive miscarriages which define as repeated pregnancy loss (RPL). Introducing the assisted reproductive technologies (ARTS) made a chance for infertile couples to solve their childless problem.
Objective:  This study was conducted to evaluate the incidence of Y-chromosome AZF region's micro-deletions in male partners of couples with recurrent miscarriage (RM).
Materials and Methods:  Thirty male partner of couples with RM and thirty infertile males, who referred to the Yazd Research and Clinical Center for Infertility were recruited to this study. In addition, 30 healthy men were screened as a control group from the same center. After DNA extraction using salting out method, the multiplex-PCR was done for amplifying 8 known STSs proximal to the AZF region of the Y-chromosome. The results were compared between the groups using Fisher's exact t-test and p<0.05 was considered statistically significant.
Results:  Of the 30 infertile males, 5 (16.6%) cases were associated with the AZF region micro-deletions of DYF87S, DYF84S1, DYF83S1 and DYF51S1, STSs. But in the fertile and RM male groups was found no deletions similar to those, of the infertile males (p=1.0). Instead 4 (13.3%) cases of the RM group males had different micro-deletions included DYS220 (AZFb, sY129), DYS262, DYF8551, and DYF8651, STSs. The AZFc locus of Y-chromosome micro-deletions have a significant role in RM (p=0.045).
Conclusion:  It seems that the Y-chromosome AZF region's micro-deletions are associated with RM, and we recommend adding this AZF region STSs into infertility analyzing panels.
Full-Text [PDF 432 kb]   (94 Downloads) |   |   Full-Text (HTML)  (25 Views)  
Type of Study: Original Article |

1. Maurer B, Gromoll J, Simoni M, Nieschlag E. Prevalence of Y chromosome microdeletions in infertile men who consulted a tertiary care medical centre:the MuS nster experience. Andrologia 2001; 33: 27-33. [DOI:10.1046/j.1439-0272.2001.00406.x]
2. Massart NA, Lissens W, Tournaye H, Stouff K. Genetic causes of spermatogenic failure. Asian J Andrology 2012; 14: 40-48. [DOI:10.1038/aja.2011.67]
3. Elizabeth E. Puschecka and Rajasingam S. Jeyendran.The impact of male factor on recurrent pregnancy loss. Int J Hum Genet 2007; 19: 222-228.
4. Bhasin S, de Kretser DM, Baker HW. Clinical review Y Pathophysiology and natural history of male infertility. J Clin Endocrinol Metab 1994; 79: 1525-1529.
5. Wettasinghe TK, Jayasekara RW, Dissanayake VH. Y chromosome microdeletions are not associated with spontaneous recurrent pregnancy loss in a Sinhalese population in Sri Lanka. Hum Reprod 2010; 25: 3152-3156. [DOI:10.1093/humrep/deq271]
6. Stephenson M, Kutteh W. Evaluation and management of recurrent early pregnancy loss. Clin Obstet Gynecol 2007; 50: 132-145. [DOI:10.1097/GRF.0b013e31802f1c28]
7. Repping S, van Daalen SK, Korver CM, Brown LG, Marszalek JD, Gianotten J, et al. A. family of human Y chromosomes has dispersed throughout northern Eurasia despite a 1.8-Mb deletion in the azoospermia factor c region. Genomics 2004; 83: 1046-1052. [DOI:10.1016/j.ygeno.2003.12.018]
8. Simoni M, Bakker E, Krausz C. EAA/EMQN best practice guidelines for molecular diagnosis of y-chromosomal microdeletions. State of the art. Int J Androl 2004; 27: 240-249. [DOI:10.1111/j.1365-2605.2004.00495.x]
9. Saliminejad K, Khorshid HR. Methodological errors in screening of Yq microdeletion in Iranian azoospermic men. Indian J Med Res 2012; 135: 137-138. [DOI:10.4103/0971-5916.93438]
10. Mirfakhraie R, Mirzajani F, Kalantar SM, Montazeri M, Salsabili N, Pourmand GR, et al. High prevalence of AZFb microdeletion in Iranian patients with idiopathic non-obstructive azoospermia. Indian J Med Res 2010; 132: 265-270.
11. Simoni ME, Bakker E, Krausz C. EAA/EMQN best practice guidelines for molecular diagnosis of y-chromosomal microdeletions. State of the art 2004. Int J Androl 2004; 27: 240-249. [DOI:10.1111/j.1365-2605.2004.00495.x]
12. Sadeghi-Nejad H, Farrokhi F. Genetics of Azoospermia: Current Knowledge, Clinical Implications, and Future Directions. Part II Y Chromosome Microdeletions. Urol J 2007; 4: 192-206.
13. Totonchi M, Mohseni Meybodi A, Borjian Boroujeni P, Sedighi Gilani M, Almadani N, Gourabi H. Clinical data for 185 infertile Iranian men with Y-chromosome microdeletion. J Assist Reprod Genet 2012; 29: 847-853. [DOI:10.1007/s10815-012-9798-7]
14. Akbari Asbagh F, Sina A, Najmabadi H, Akbari MT, Tabarroki A, Pourmand Gh. Prevalanceof Y chromosome microdeletions in Iranian infertile men. Acta Medica Iranica 2003; 41: 164-170.

Send email to the article author

© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb