Volume 11, Issue 1 (4-2013)                   IJRM 2013, 11(1): 65-0 | Back to browse issues page

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Nikzad H, Haddad Kashani H, Kabir-Salmani M, Akimoto Y, Iwashita M. Expression of galectin-8 on human endometrium: Molecular and cellular aspects. IJRM. 2013; 11 (1) :65-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-335-en.html
1- Anatomical Sciences Research Center, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran, Iran , nikzad_h@kaums.ac.ir
2- Anatomical Sciences Research Center, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran, Iran
3- Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran, Iran
4- Department of Anatomy, School of Medicine, Kyorin University, Mitaka, Tokyo, Japan, Japan
5- Department of Obstetrics and Gynecology, School of Medicine, Kyorin University, Mitaka, Tokyo, Japan, Japan
Abstract:   (133 Views)
Background: The up-regulation of galectin-3, galectin-9, and galectin-15 expression in the luminal and glandular epithelium was reported in preparation of the endometrium for embryo implantation at the midlutheal phase. However, no data was available regarding the expression and the distribution pattern of galectin-8 in the human endometrium during a regular menstrual cycle.
Objective: The current study designed to investigate the expression and the distribution pattern of galectin-8, a beta-galactoside-binding lectin in the human endometrium during both proliferative and luteal phases of a regular menstrual cycle.
Materials and Methods: Endometrial biopsies were obtained from the anterior wall of the uterine cavity of 16 women (proliferative phase: n=4, lutheal phase: n=12). All female patients with mean age of 37.5 years were fertile (range 25-45) . Each biopsy was divided into three pieces; one piece was fixed in formaldehyde for light microscopy and immunohistochemistry. The second portion fixed in glutaraldehyde for scanning electron microscopy and the third portion was prepared for western blot analysis.
Results: Data of immunoblotting revealed a molecular weight of 34 kD band with high intensity in the lutheal phase samples. The immunohistochemistry staining demonstrated that galectin-8 expressed at a very low concentration during the proliferative phase, but showed a high expression throughout the lutheal phase. The expression of galectin-8 observed in luminal surface epithelium, glandular epithelium and stroma.
Conclusion: The up-regulation of the expression of galectin-8 during lutheal phase may suggest galectin-8 as one of the potential molecular marker of the endometrial receptivity. These data propose that galectin-8 may play an important role during the initial events of human embryo implantation.
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Type of Study: Original Article |
Received: 2017/10/1 | Accepted: 2018/02/24 | Published: 2018/02/24

References
1. Carson DD, Bagchi I, Dey SK, Enders AC, Fazleabas AT, Lessey BA, et al. Embryo implantation. Dev Biol 2000; 223: 217-237. [DOI:10.1006/dbio.2000.9767]
2. Nikas G, Develiglu OH, Toner JP, Jones HWJ. Endometrial pinopodes indicate a shift in the window of receptivity in IVF cycles. Hum Reprod 1999; 14: 787-792. [DOI:10.1093/humrep/14.3.787]
3. Nardo LG, Sabatini L, Rai R, Nardo F. Pinopode expression during human implantation. Eur J Obstet Gynecol Reprod Biol 2002; 101: 104-108. [DOI:10.1016/S0301-2115(01)00523-1]
4. Lindhard A, Bentin-Ley U, Ravn V, Islin H, Hviid T, Rex S, et al. Biochemical evaluation of endometrial function at the time of implantation. Fertil Steril 2002; 78: 221-233. [DOI:10.1016/S0015-0282(02)03240-5]
5. Cavagna M, Mantese JC. Biomarkers of endometrial receptivity-A review. Placenta 2003; 24: s39-s47.
6. Kabir-Salmani M, Hosseini A, Rezazadeh Valojerdi M. Endometrial Receptivity to Implantation in Humans:Biochemical and Molecular Aspects. Cell Journal 2008; 10: 1-24.
7. Barondes SH, Cooper, DNW, Gitt MA, Leffler H. Galectins: Structure and function of a large family of animal lectins. J Biol Chem 1994; 269: 20807-20810.
8. Bidon N, Brichory F, Bourguet P, Le Pennec JP, Dazord L. Galectin-8: a complex sub-family of galectins. Int J Mol Med 2001; 8: 245-250. [DOI:10.3892/ijmm.8.3.245]
9. Gray CA, Dunlap KA, Burghardt RC, Spencer .E. Galectin-15 in ovine uteroplacental tissues. Reproduction 2005; 130: 231-240. [DOI:10.1530/rep.1.00637]
10. Hadari YR, Paz K, Dekel R, Mestrovic T, Accili D, Zick Y. Galectin-8: a new rat lectin related to galectin-4. J Biol Chem 1995; 270: 3447-3453. [DOI:10.1074/jbc.270.7.3447]
11. Liu FT, Patterson RJ, Wang JL. Intracellular functions of galectins. Biochim Biophys Acta 2002; 1572: 263-273. [DOI:10.1016/S0304-4165(02)00313-6]
12. Bassen R, Brichory F, Cahlet-Maugendre S, Bidon N, Delaval P, Desrues B, et al. Expression of Po66-CBP, a type-8 galectin, in different healthy, tumoral and peritumoral tissues. Anticancer Res 1999; 19: 5429-5433.
13. Hughes RC. Galectins as modulators of cell adhesion. Biochimie 2001; 83: 667-676. [DOI:10.1016/S0300-9084(01)01289-5]
14. Zick Y, Eisenstein M, Goren RA, Hadari YR, Levy Y, Ronen D. Role of galectin-8 as a modulator of cell adhesion and cell growth. Glycoconj J 2004; 19: 517-526. [DOI:10.1023/B:GLYC.0000014081.55445.af]
15. Hadari YR, Arbel-Goren R, Levy Y, Amsterdam A, Alon R, Zakut R, et al. Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis. J Cell Sci 2000; 113: 2385-2397.
16. Levy Y, Arbel-Goren R, Hadari YR, Eshhar S, Ronen D, Elhanany E, et al. Galectin-8 functions as a matricellular modulator of cell adhesion. J Biol Chem 2001; 276: 31285-31295. [DOI:10.1074/jbc.M100340200]
17. Lessey BA, Ilessami AO, Lessey MA, Riben M, Harris JE, Chwalisz K. Luminal and glandular endometrial epithelium express integrins differentially throught the menstrual cycle: Implications for implantation , contraception, and infertility. Am J Reprod Immunol 1996; 35: 195-204. [DOI:10.1111/j.1600-0897.1996.tb00031.x]
18. von Wolff M, Wang X, Gabius HJ, Strowitzki T. Galectin fingerprinting in human endometrium and decidua during the menstrual cycle and in early gestation. Mol Hum Reprod 2005; 11: 189-194. [DOI:10.1093/molehr/gah144]
19. Nikzad H, Kabir-Salmani M, Shiokawa S, Akimoto Y, Iwashita M. Co-expression of galectin-3 and αvβ3 integrin at pinopodes of human endometrium. Iran J Reprod Med 2010; 4: 145-152.
20. Popovici RM, Krause MS, Germeyer A, Strowitzki T, von Wolff M. Galectin-9: a new endometrial epithelial marker for the mid- and late-secretory and decidual phases in humans. J Clin Endocrinol Metab 2005; 90: 6170-6176. [DOI:10.1210/jc.2004-2529]
21. Shimizu Y, Kabir-Salmani M, Azadbakht M, Sugihara K, Sakai K, Iwashita M. Expresssion and localization of galectin-9 in the human uterodome. Endocr J 2008; 55: 879-887. [DOI:10.1507/endocrj.K08E-111]
22. Satterfield MC, Bazer FW, Spencer TE. Progesterone regulation of preimplantation conceptus growth and galectin 15 (LGALS15) in the ovine uterus. Biol Reprod 2006; 75: 289-296. [DOI:10.1095/biolreprod.106.052944]
23. Farmer JL, Burghardt RC, Jousan FD, Hansen PJ, Bazer FW, Spencer TE. Galectin 15 (LGALS15) functions in trophectoderm migration and attachment. FASEB J 2008; 22: 548-560. [DOI:10.1096/fj.07-9308com]
24. Noyes RW, Hertig AT, Rock J. Dating the endometrial biopsy. Am J Obstet Gynecol 1975; 122: 262-263. [DOI:10.1016/S0002-9378(16)33500-1]
25. Croxatto HB, Ortiz ME, Diaz S, Hess R, Balmaceda J, Croxatto HD. Studies on the duration of egg transport by the human oviduct. II. Ovum location at various intervals following luteinizing hormone peak. Am J Obstet Gynecol 1978; 132: 629-634. [DOI:10.1016/0002-9378(78)90854-2]
26. Bergh PA, Navot D. The impact of embryonic development and endometrial maturity on the timing of implantation. Fertil Steril 1992; 58: 537-542.
27. Norwitz ER, Schust DJ, Fisher SJ. Implantation and the survival of early pregnancy. N Engl J Med 2001; 345: 1400-1408. [DOI:10.1056/NEJMra000763]
28. Genbacev OD, Prakobphol A, Foulk RA, Krtolica AR, Ilic D, Singer MS, et al. Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface. Science 2003; 299: 405-408. [DOI:10.1126/science.1079546]
29. Nishi N, Shoji H, Seki M, Itoh A, Miyanaka H, Yuube K, et al. Galectin-8 modulates neutrophil function via interaction with integrin αM. Glycobiology 2003; 13: 755-763. [DOI:10.1093/glycob/cwg102]
30. Hadari YR, Arbel-Goren R, Levy Y, Amsterdam A, Alon R, Zakut R, et al. Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis. J Cell Sci 2000; 113: 2385-2397.
31. Cárcamo C, Pardo E, Oyanadel C, Bravo-Zehnder M, Bull P, Cáceres M, et al. Galectin-8 binds specific β1 integrins and induces polarized spreading highlighted by asymmetric lamellipodia in Jurkat T-cells. Exp Cell Res 2006; 312: 374-386. [DOI:10.1016/j.yexcr.2005.10.025]

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