Volume 10, Issue 3 (7-2012)                   IJRM 2012, 10(3): 181-192 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Razi M, Najafi G, Feyzi S, Karimi A, Shahmohamadloo S, Nejati V. Histological and histochemical effects of Gly-phosate on testicular tissue and function. IJRM. 2012; 10 (3) :181-192
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-284-en.html
1- Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , Mazdak.razi@gmail.com
2- Department of Anatomy, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
3- Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
4- Departemt of Biology Sciences, Faculty of Basic Sciences, Urmia University, Urmia, Iran
Abstract:   (1475 Views)
Background: In this study we aimed to evaluate the impact of chronic exposure to the Gly-phosate (GP) on rat’s testicular tissue and sperm parameters. Objective: Testicular tissue, morphology of sperms and testosterone level in serum of mature male rats were analyzed.
Materials and Methods: Animals were divided into two test and control-sham groups. The test group was subdivided into 4 groups (10, 20, 30 and 40 days GP administrated). Each test group (n=8) received the compound at dose of 125 mg/kg, once a day, orally for 40 days while control-sham group (n=16) received the corn oil (0.2 ml/day).
Results: Microscopic analyses revealed increased thickness of tunica albuginea, obvious edema in sub-capsular and interstitial connective tissue, atrophied seminiferous tubules, arrested spermatogenesis, negative tubular differentiation and repopulation indexes, decreased Leydig cells/mm2 of interstitial tissue, hypertrophy and cytoplasmic granulation of Leydig cells, elevated death, immature sperm and increased immotile and abnormal sperm percentage. The carbohydrate ratio was reduced in first three layers of the germinal epithelium (GE) cytoplasm. The upper layers of the GE series were manifested with low rate of lipid accumulation in cytoplasm, while the cells which were located in first layers were revealed with higher amount of lipid foci. Hematological investigations showed significant (p<0.05) decreasing of testosterone level in serum.
Conclusion: The current data provide inclusive histological feature of chronic exposure against GP with emphasizing on reproductive disorders including histological adverse effect on the testicular tissue, spermatogenesis, sperm viability and abnormality which potentially can cause infertility.
Full-Text [PDF 1055 kb]   (203 Downloads) |   |   Full-Text (HTML)  (140 Views)  
Type of Study: Original Article |

References
1. De Bleecker J, Van den Neucker K, Colardyn F. Intermediate syndrome in organophosphorous poisoning; a prospective study. Crit Care Med 1993; 21: 1706-1711. [DOI:10.1097/00003246-199311000-00020]
2. Slotkin TA, Seidler FJ. Comparative developmental neurotoxicity of organophosphates in vivo: Transcriptional responses of pathways for brain cell development, cell signaling, cytotoxicity and neurotransmitter systems. Brain Res Bull 2007; 72: 232-274. [DOI:10.1016/j.brainresbull.2007.01.005]
3. Woo YT, Lai DY, Fergusson MF, Arcos JC. Carcinogenicity of organophosphorus compounds: an analysis of their structure activity relationship. Environ carcinogen Ecotoxicol Rev 1996; 16: 39-42. [DOI:10.1080/10590509609373479]
4. Fattahi E, Parivar K, Jorsaraei SGA, Moghadamnia AK. The effects of diazinon on testosterone, FSH and LH levels and testicular tissue in mice. Iran J Reprod Med 2009; 7: 59-64.
5. Zahran MM, Abdel-Aziz KB, Abdel-Raof A, Nahas EM. The Effect of Subacute Doses of Organophosphorus Pesticide, Nuvacron, on the Biochemical and Cytogenetic Parameters of Mice and Their Embryos. Res J Agricul Biol Scien 2005; 1: 277-283.
6. Bonilla E, Hernández F, Cortés L, Mendoza M, Mejía J, Carrillo E, et al. Effects of the insecticides malathion and diazinon on the early oogenesis in mice in vitro. Environ Toxicol 2008; 23: 240-245. [DOI:10.1002/tox.20332]
7. Adamkovicova M, Toman R, Cabaj M. Diazinon and Cadmium acute testicular toxicity in rats examined by histopathological and morphological methods. Slovak J Anim Sci 2010; 43: 134-140.
8. Kolstad HA, Bonde JP, Spano M, Giwercman A, Zschiesche W, Kaae D. Change in semen quality and sperm chromatin structure following occupational styrene exposure. Int Arch Occup Environ Health 1999; 7: 135-141. [DOI:10.1007/s004200050352]
9. Swan SH, Kruse RL, Liu F, Barr DB, Drobnis EZ, Redmon JB, et al. Study for Future Families Research Group. Semen quality in relation to biomarkers of pesticide exposure. Environ Health Perspect 2003; 111: 1478-1484. [DOI:10.1289/ehp.6417]
10. Dutta HM, Meijer HJ. Sublethal effects of diazinon on the structure of the testis of bluegill, Lepomis macrochirus: a microscopic analysis. Environ pollut 2003; 125: 355-360. [DOI:10.1016/S0269-7491(03)00123-4]
11. Maxwell LB, Dutta HM. Diazinon-induced endocrine disruption in bluegill sunfish, Lepomis macrochirus. Ecotoxicol Environ Saf 2005; 60: 21-27. [DOI:10.1016/j.ecoenv.2003.12.015]
12. Wesseling C, Antich D, Hogstedt C, Rodríguez AC, Ahlbom A. Geographical differences of cancer incidence in Costa Rica in relation to environmental and occupational pesticide exposure. Int J Epidemiol 1999; 28: 365-374. [DOI:10.1093/ije/28.3.365]
13. Malekinegad H, Mirzakhani N, Razi M, Cheraghi H, Alizadeh A, Dardmeh F. Protective effects of Melatonin and Glycyrrhiza glabra Extract on Ochratoxin A-Induced Detrimental impact on Testes in Mature Male Rats. Hum Exp Toxicol 2010; 29: 110-123.
14. Bustos-Obregón E, González-Hormazabal P. Effect of a single dose of malathion on spermatogenesis in mice. Asian J Androl 2003; 5: 105-107.
15. Pina-Guzman B, Solis-Heredia MJ, Quintanilla-Vega B. Diazinon alters sperm chromatin structure in mice by phosphorylating nuclear protamines. Toxicol Appl Pharmacol 2005; 202: 189-198. [DOI:10.1016/j.taap.2004.06.028]
16. Suzuli N, Sofikitis N. Protective Effects of Antioxidants on Testicular Functions of Varicocelized Rats. Yonago Acta medica 1999; 42: 87-94.
17. Walsh LP, McCormick C, Martin C, Stocco DM. Roundop inhibits steroidogenesis by disrupting steroidogenic acute regulatory (star) protein expression. Environ Health Perspect 2000; 108: 769-776. [DOI:10.1289/ehp.00108769]
18. Pant N, Srivastava SP. Testicular and spermatotoxic effect of quinaphos in rats. J Appl Toxicol 2003; 23: 271-274. [DOI:10.1002/jat.919]
19. World Health Organization. Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. 4th Ed. Cambridge: Cambridge University Press; 1999: 14-17.
20. Khan IA, Reddy BV, Mahboob M, Rahman MF, Jamil K. Acute and subacute effects of 2-butenoic acid-3- (diethooxyphosphinothiol) methylester (RPR-I) on testis of albino rat. Ind J Exp Biol 2002; 40: 162-168.
21. Sobarzo C, Bustos-obregon E. Acute effect of parathion on the seminiferous epithelium of immature mice. Rivista chilena de anatomia 2000; 18: 1-9. [DOI:10.4067/S0716-98682000000100008]
22. Prashanthi N, Narayana K, Nayanatara A, Chandra Kumar HH, Bairy KL, D'Souza UJ. The reproductive toxicity of the organophosphate pesticide 0, 0-dimethyl 0-4- nitrophenyl phosphorothioate (methyl parathion) in the male rat. Folia Morphol (Warsz) 2006; 65: 309-321.
23. Shan L, Hardy DO, Catterall JF, Hardy MP. Effects of luteinizing hormone (LH) and androgen on steady state levels of messenger ribonucleic acid for LH receptors, androgen receptors, and steroidogenic enzymes in rat Leydig cell progenitors in-vivo. Endocrinology 1995; 136: 1686-1693. [DOI:10.1210/endo.136.4.7895679]
24. Sarkar R, Mohanakumar KP, Chowdhury M. Effects of an organophosphate pesticide, quinalphos, on the hypothalamo- pituitary- gonadal axis in adult male rats. J Reprod Fertil 2000; 118: 29-38. [DOI:10.1530/reprod/118.1.29]
25. Hess RA, Nakai M. Histopathology of the male reproductive system induced by the fungicide benomyl. Histol Histopathol 2000; 15: 207-224
26. Boekelheide K, Schoenfeld HA. Spermatogenesis by Sisyphus: proliferating stem germ cells fail to repopulate the testis after 'irreversible' injury. Adv Exp Med Biol 2001; 500: 421-428. [DOI:10.1007/978-1-4615-0667-6_64]
27. Izumi H, Kimura E, Ota T, Shimazu S. A two-generation reproductive toxicity study of n-butylbenzene in rats. J Toxicol Sci 2005; 30: 21-38. [DOI:10.2131/jts.30.S21]
28. Chapin RE, Phelps JL, Somkuti SG, Heindel JJ, Burka LT. The interaction of Sertoli and Leydig cells in the testicular toxicity of tri-o-cresyl phosphate. Toxicol Appl Pharmacol 1990; 104: 483-495. [DOI:10.1016/0041-008X(90)90170-Y]
29. Skinner MK, Fritz IB. Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions. Proc Natl Acad Sci U S A 1985; 82: 114-118. [DOI:10.1073/pnas.82.1.114]
30. Goldman JD, Rehnberg GL, Cooper RL, Gray LE, Hein JF, McElroy WK. Effects of the benomyl metabolite, carbendazim, on the hypothalamic- pituitary reproductive axis in the male rat. Toxicology 1989; 57: 173-182. [DOI:10.1016/0300-483X(89)90163-7]
31. Kishimoto K, Fukuyado T, Sawamoto O, Kurisu K. Influence of daily subcutaneous administration of reserpine for 4 weeks or 9 weeks before mating on testis, sperm and male fertility in rats. J Toxicol Sci 1995; 20: 367-374. [DOI:10.2131/jts.20.367]
32. Kerr JB, Mayberry RA, Irby DC. Morphometric studies on lipid inclusions in Sertoli cells during the spermatogenic cycle in the rat. Cell Tissue Res 1984; 236: 699-709. [DOI:10.1007/BF00217241]
33. Farooqi IS, Rahilly SO. Monogenic Human Obesity Syndromes. Recent Prog Horm Res 2004; 59: 409-424. [DOI:10.1210/rp.59.1.409]
34. Civen M, Brown CB. The effect of organophosphate insecticides on adrenal corticosterone formation. Pes Biochem Physiol 1974; 4: 254-259. [DOI:10.1016/0048-3575(74)90108-4]
35. Najafi GR, Razi M, Hoshyar A, Shahmohamadloo S, Feyzi S. The Effect of Chronic Exposure with Imidaclopride Insecticide on Fertility in Mature Male Rats. Int J Fertil Steril 2010; 9: 9-16.
36. Ibrahim NA, El-Gamal BA. Effect of diazinon, an organophosphate insecticide, on plasma lipid constituents in experimental animals. J Biochem Mol Biol 2003; 36: 499-504. [DOI:10.5483/BMBRep.2003.36.5.499]
37. Duru NK, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 2000; 74: 1200-1207. [DOI:10.1016/S0015-0282(00)01591-0]
38. Najafi GR, Razi M, Hoshyar A, Shahmohamadloo S, Feyzi S. The Effect of Chronic Exposure with Imidaclopride Insecticide on Fertility in Mature Male Rats. Int J Steril Fertil 2010; 9: 9-16.
39. Khaik A, Fathiazad F, Nouri M, Khaki AA, Ozanc CC, Ghafari-Novin M, et al. The effects of Ginger on spermatogenesis and sperm parameters of rat. Iran J Reprod Med 2009; 7: 7-12.
40. Sharma PK, Agarwal A. A Role of reactive oxygen species in male infertility. Urology 1996; 48: 835-850. [DOI:10.1016/S0090-4295(96)00313-5]
41. Watanabe HK, Hoskins B, Ho IK. Selective inhibitory effect of organophosphates on UDP-glucuronyl transferase activities in rat liver microsomes. Biochem Pharmacol 1986; 35: 455-460. [DOI:10.1016/0006-2952(86)90219-4]
42. Bustos-Obregon E, Gonzalez JR. Melatonin as protective agent for the cytotoxic effects of diazinon in the spermatogenesis in the earthworm Eisenia foetida. Ital J Anat Embryol 2005; 110: 159-165.

Send email to the article author


© 2021 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb