Volume 10, Issue 2 (7-2012)                   IJRM 2012, 10(2): 77-0 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Aliabadi E, Soleimani Mehranjani M, Borzoei Z, Talaei-Khozani T, Mirkhani H, Tabesh H. Effects of L-carnitine and L-acetyl-carnitine on testicular sperm motility and chromatin quality. IJRM. 2012; 10 (2) :77-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-271-en.html
1- Department of Anatomy, Shiraz University of Medical Sciences (SUMS), Shiraz, Iran , mahzad626@yahoo.com
2- Department of Biology, Faculty of Sciences, University of Arak, Arak, Iran
3- Department of Anatomy, Shiraz University of Medical Sciences (SUMS), Shiraz, Iran
4- Department of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran
5- Department of Biostatistics, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (547 Views)
Background: Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality.
Objective: The aim of this investigation was to evaluate the improvement of the testicular sperm motility and chromatin quality exposed to L-carnitine (LC) and L-acetyl-carnitine (LAC), which are normally concentrated in testis and epididymis, compared with Pentoxifylline (PF), which used for sperm motility enhancement in IVF procedures.
Materials and Methods: TESE samples from 30 male mice divided into four parts. The sperm samples were added to Ham' F10 (control) or the media contained 1.76mM of LC, LAC or PF), then, the samples were kept in the room temperature for 30, 90 and 180 min. At each time step, sperm motility and chromatin quality were assessed. Chromatin quality was evaluated by chromomycin A3 and aniline blue. Statistical analysis was performed using one way analysis of variance (ANOVA). A p-value less than 0.05 were accepted as a statistically significant difference.
Results: The results showed LC, LAC and PF significantly increased the sperm motility. However, sperm chromatin quality only improved significantly by administration of LC and LAC.
Conclusion: Administration of LC and LAC to the testicular sperm samples can lead to improve both sperm motility and chromatin quality. It may be because they can mimic in vivo sperm condition during late spermatogenesis.
Full-Text [PDF 395 kb]   (113 Downloads) |   |   Full-Text (HTML)  (56 Views)  
Type of Study: Original Article |

References
1. Isidori AM, Pozza C, Gianfrilli D, Isidori A. Medical treatment to improve sperm quality. Reprod Biomed Online 2006; 12: 704-714. [DOI:10.1016/S1472-6483(10)61082-6]
2. Su LM, Palermo GD, Goldstein M, Veeck LL, Rosenwaks Z, Schlegel PN. Testicular sperm extraction with intracytoplasmic sperm injection for nonobstructive azoospermia: testicular histology can predict success of sperm retrieval. J Urol 1999; 161: 112-116. [DOI:10.1016/S0022-5347(01)62079-2]
3. Jeulin C, Lewin LM. Role of free L-carnitine and acetyl-L-carnitine in post-gonadal maturation of mammalian spermatozoa. Hum Reprod Update 1996; 2: 87-102. [DOI:10.1093/humupd/2.2.87]
4. Golan R, Cooper TG, Oschry Y, Oberpenning F, Schulze H, Shochat L, et al. Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry. Hum Reprod 1996; 11: 1457-1462. [DOI:10.1093/oxfordjournals.humrep.a019419]
5. Liu J, Tsai Y, Katz E, Compton G, Garcia GE, Baramki TA. Outcome of in-vitro culture of fresh and frozen–thawed human testicular spermatozoa. Hum Reprod 1997; 12: 1667-1672. [DOI:10.1093/humrep/12.8.1667]
6. Jones RC, Dacheux JL, Nixon B, Ecroyd HW. Role of the epididymis in sperm competition. Asian J Androl 2007; 9: 493-499. [DOI:10.1111/j.1745-7262.2007.00284.x]
7. Pruneda A, Yeung CH, Bonet S, Pinart E, Cooper TG. Concentrations of carnitine, glutamate and myo-inositol in epididymal fluid and spermatozoa from boars. Anim Reprod Sci 2007; 97: 344-355. [DOI:10.1016/j.anireprosci.2006.01.013]
8. Kobayashi D, Goto A, Maeda T, Nezu JI, Tsuji A, Tamai I. OCTN2-mediated transport of carnitine in isolated Sertoli cells. Reproduction 2005; 129: 729-736. [DOI:10.1530/rep.1.00507]
9. Abdelrazik H, Agrawal A. L-carnitine and assisted reproduction. Arch Med Sci 2009; 5: 43-47.
10. Kanter M, Topcu-Tarladacalisir Y, Parlar S. Antiapoptotic effect of L-carnitine on testicular irradiation in rats. J Mol Histol 2010; 41: 121-128. [DOI:10.1007/s10735-010-9267-5]
11. Tunc O, Thompson J, Tremellen K. Improvement in sperm DNA quality using an oral antioxidant therapy. Reprod Biomed Online 2009; 18: 761-768. [DOI:10.1016/S1472-6483(10)60024-7]
12. De Iuliis GN, Thomson LK, Mitchell LA, Finnie JM, Koppers AJ, Hedges A, et al. DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress. Biol Reprod 2009; 81: 517-524. [DOI:10.1095/biolreprod.109.076836]
13. Balaban B, Urman B, Isiklar A, Alatas C, Aksoy S, Mercan R, Nuhoglu A. Progression to the blastocyst stage of embryos derived from testicular round spermatids. Hum Reprod 2000; 15: 1377-1382. [DOI:10.1093/humrep/15.6.1377]
14. Sato M, Ishikawa A. Room temperature storage of mouse epididymal spermatozoa: exploration of factors affecting sperm survival. Theriogenology 2004; 61: 1455-1469. [DOI:10.1016/j.theriogenology.2003.07.013]
15. Kovacic B, Vlaisavljevic V, Reljic M. Clinical Use of Pentoxifylline for Activation of Immotile Testicular Sperm before ICSI in Patients with Azoospermia. Androl 2006; 2: 45-52. [DOI:10.2164/jandrol.05079]
16. World Health Organization. WHO Laboratory manual for examination of human semen and sperm-cervical mucuinteraction. 4th Ed. Cambridge; Cambridge University Press; 2010.
17. Moreira Jr, Sergio G, Lipshult z, Larry I. Management of Male Infertility. TSWJ 2004; 4: 214-448.
18. Terquem A, Dadoune JP. Aniline blue staining of human spermatozoa chromatin Evaluation of nuclear maturation. J Androl(ed) J sperm cell 1983; 249-252.
19. Lolis D, Georgiou I, Syrrou M, Zikopoulos K, Konstantelli M, Messinis I. Chromomycin A3 staining as an indicator of protamine deficiency and fertilization. Int Androl 1996; 19: 23-27. [DOI:10.1111/j.1365-2605.1996.tb00429.x]
20. Lenzi A, Sgrò P, Salacone P, Paoli D, Gilio B, Lombardo F, et al. Aplacebo-controlleddouble-blind randomized trial of the use of combined L-carnitine and L-acetyl-carnitine treatment in men with asthenozoospermia. Fertil Steril 2004; 81: 1578-1584. [DOI:10.1016/j.fertnstert.2003.10.034]
21. Tasdemir I, Tasdemir M, Tavukcuoglu S. Effect of pentoxifylline on immotile testicular spermatozoa. Assist Reprod Genet 1998; 15: 90-92. [DOI:10.1007/BF02766832]
22. Tanphaichitr N. In vitro stimulation of human sperm motility by acetylcarnitine biological chemistry. Int J Fertility 1977; 22: 85-91.
23. Shi JZ, Zhang SS, Zhang Z, Liang Q, Shi Y, Hua JL, et al. Expressions of sperm-specific genes in carnitine-cultured testis sperm of obstructive azoospermia patients. Zhonghua Nan Ke Xue 2010; 16: 504-509.
24. Aitken RJ, Baker MA. Oxidative stress, sperm survival and fertility control. Mol Cel Endocrinol 2006; 250: 66-69. [DOI:10.1016/j.mce.2005.12.026]
25. Solarska K, Lewińska A, Karowicz-Bilińska A, Bartosz G.The antioxidant properties of carnitine in vitro. Cell Mol Biol Lett 2010; 15: 90-97. [DOI:10.2478/s11658-009-0036-y]
26. Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil 1987; 81: 459-469. [DOI:10.1530/jrf.0.0810459]
27. de Lamirande E, Gagnon C. Reactive oxygen species and human spermatozoa. II Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J Androl 1992; 13: 379-386.
28. Dokmeci D. Oxidative stress, male infertility and the role of carnitines. Folia Med (Plovdiv) 2005; 47: 26-30.
29. Brooks DE. Carnitine, acetylcarnitine and the activity of carnitine acyltransferases in seminal plasma and spermatozoa of men, rams and rats. Reprod Fertil 1979; 56: 667-673. [DOI:10.1530/jrf.0.0560667]
30. Casillas ER, Chaipayungpan S. The distribution of carnitine and acetylcarnitine in the rabbit epididymis and the carnitine content of rabbit spermatozoa during maturation. Reprod Fertil 1979; 56: 439-444. [DOI:10.1530/jrf.0.0560439]
31. Emiliani S, Bergh M, Vannin A, Biramane J, Verdoodt M, Englert Y. Increased sperm motility after in-vitro culture of testicular biopsies from obstructive azoospermic patients results in better post-thaw recovery rate. Hum Reprod 2000; 1: 2371-2374. [DOI:10.1093/humrep/15.11.2371]
32. Tanaka H, Baba T. Gene expression in spermiogenesis. Cell Mol Life Sci 2005; 62: 344-354. [DOI:10.1007/s00018-004-4394-y]
33. Vilfan ID, Conwell C, Hud NV. Formation of native-like mammaliansperm cell chromatin with folded bull protamine. Biol Chem 2004; 279: 20088-20095. [DOI:10.1074/jbc.M312777200]
34. Hammadeh ME, Al-Hasani S, Doerr S, Stieber M, Rosenbaum P, Schmidt W, et al. Comparison between chromatin condensation and morphology from testis biopsy extracted and ejaculated spermatozoa and their relationship to ICSI outcome. Hum Reprod 1999; 14: 363-367. [DOI:10.1093/humrep/14.2.363]
35. Kodama H, Yamaguchi R, Fukuda J, Kasai H, Tanaka T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 1997; 68: 519-524. [DOI:10.1016/S0015-0282(97)00236-7]
36. Menezo YJ, Hazout A, Panteix G, Robert F, Rollet J, Cohen-Bacrie P, et al. Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect. Reprod Biomed Online 2007; 14: 418-421. [DOI:10.1016/S1472-6483(10)60887-5]
37. Shamsi MB, Kumar R, Dada R. Evaluation of nuclear DNA damage in human spermatozoa in men opting for assisted reproduction. Ind J Med Res 2008; 127: 115-123.
38. Jeulin CM, Lewin L. Role of free L-carnitine and acetyl-L-carnitine in post-gonadal maturation of mammalian spermatozoa. Hum Reprod 1996; 2: 87-102.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2019 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb