Volume 17, Issue 2 (February 2019 2019)                   IJRM 2019, 17(2): 79-88 | Back to browse issues page

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Sargazi Z, Nikravesh M R, Jalali M, Sadeghnia H R, Rahimi Anbarkeh F. The protective effect of vitamin E on rats’ ovarian follicles following an administration of diazinon: An experimental study. IJRM. 2019; 17 (2) :79-88
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1397-en.html
1- Department of Basic Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
2- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , Nikraveshmr@mums.ac.ir
3- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (471 Views)
Background: Diazinon (DZN) is an organophosphate insecticide that has been widely utilized in agriculture all over the world and caused many negative effects on different species such as plants and animal species, especially on a human.
Objective: The aim of the present study was to evaluate the protective effect of vitamin E on rats’ ovarian follicles following an administration of  diazinon.
Materials and Methods: A total of 30 adult female Wistar rats were divided into five groups: a control group (without any intervention), sham group (received only pure olive oil, as solvent), experimental group I (DZN+olive oil, 60 mg/kg), experimental group II (vitamin E, 200 mg/kg), and experimental group III (DZN: 60 mg/kg+vitamin E: 200 mg/kg). All drugs were injected intraperitoneally, except vitamin E which was administrated by gavage. The animals were scarified after two weeks and left ovary was used to measure proliferation of ovarian follicles. Tissues were analyzed by the PCNA technique and viewed with an optical microscope for evaluating cells proliferation.
Results: The result of the present study revealed that the number of proliferative cells in the experimental group I decreased significantly in contrast to the control group in secondary and Graffian follicles (p< 0.001). The administration of vitamin E plus DZN significantly increased proliferative cells compared to the DZN group (p< 0.001). Primordial follicles showed that all study groups were lacking PCNA positive cells, which means no expression of PCNA in these follicles. The results of this study showed that primary follicles in all study groups had a few and scattered PCNA positive cells with no significant difference between the groups (p> 0.05).
Conclusion: Results showed that DZN reduced proliferation in secondary and Graffian follicles and vitamin E increased it. The results of this study suggested that vitamin E by its antioxidant activity was able to improve the DZN-induced ovarian toxicity.
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1. [1] Köprücü SŞ, Köprücü K, Ural MŞ, İspir Ü, Pala M. Acute toxicity of organophosphorous pesticide diazinon and its effects on behavior and some hematological parameters of fingerling European catfish (Silurus glanis L.).Pesticide Biochemistry and Physiology 2006; 86: 99–105. [DOI:10.1016/j.pestbp.2006.02.001]
2. [2] Shah MD, Iqbal M. Diazinon-induced oxidative stress and renal dysfunction in rats. Food Chem Toxicol 2010; 48: 3345–3353. [DOI:10.1016/j.fct.2010.09.003]
3. [3] Yehia MA, El-Banna SG, Okab AB. Diazinon toxicity affects histophysiological and biochemical parameters in rabbits. Exp Toxicol Pathol 2007; 59: 215–225. [DOI:10.1016/j.etp.2007.09.003]
4. [4] Jahromi VH, Koushkaki M, Kargar H. The effects of malathion insecticide on ovary in female rats. National park forschung in der schweiz (Switzerland Research Park Journal) 2012; 101: 231–235.
5. [5] Uzun FG, Kalender S, Durak D, Demir F, Kalender Y. Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem Toxicol 2009; 47: 1903–1908. [DOI:10.1016/j.fct.2009.05.001]
6. [6] Casas E, Bonilla E, Ducolomb Y, Betancourt M. Differential effects of herbicides atrazine and fenoxaprop-ethyl, and insecticides diazinon and malathion, on viability and maturation of porcine oocytes in vitro. Toxicol In Vitro 2010; 24: 224–230. [DOI:10.1016/j.tiv.2009.09.004]
7. [7] Ogutcu A, Uzunhisarcikli M, Kalender S, Durak D, Bayrakdar F, Kalender Y. The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E. Pestic Biochem Phys 2006; 86: 93–98. [DOI:10.1016/j.pestbp.2006.01.010]
8. [8] Sarabia L, Maurer I, Bustos-Obregon E. Melatonin prevents damage elicited by the organophosphorous pesticide diazinon on mouse sperm DNA. Ecotoxicol Environ Saf 2009; 72: 663–668. [DOI:10.1016/j.ecoenv.2008.04.023]
9. [9] Khaki A, Rajabzadeh A, Khaki AA. Side effects of pyrethroid and supporting role of onion in the male rat's spermatogenesis. Chin Med J 2017; 130: 3015–3016. [DOI:10.4103/0366-6999.220297]
10. [10] Kalender S, Ogutcu A, Uzunhisarcikli M, Açikgoz F, Durak D, Ulusoy Y, et al. Diazinon-induced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes. Toxicology 2005; 211: 197–206. [DOI:10.1016/j.tox.2005.03.007]
11. [11] Güney M, Demirin H, Oral B, Özgüner M, Bayhan G, Altuntas I. Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C.Hum Exp Toxicol 2007; 26 :491–498. [DOI:10.1177/0960327106077505]
12. [12] Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol 2012; 10:49–79. [DOI:10.1186/1477-7827-10-49]
13. [13] Hariri AT, Moallem SA, Mahmoudi M, Memar B, Hosseinzadeh H. Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: protective effects of crocin and safranal. Food ChemToxicol 2010; 48: 2803–2808. [DOI:10.1016/j.fct.2010.07.010]
14. [14] Kaur P, Radotra B, Minz RW, Gill KD. Impaired mitochondrial energy metabolism and neuronal apoptotic cell death after chronic dichlorvos (OP) exposure in rat brain. Neurotoxicology 2007; 28: 1208–1219. [DOI:10.1016/j.neuro.2007.08.001]
15. [15] Zhang H, Jiang X, Zhang Y, Xu B, Hua J, Ma T, et al. microRNA 376a regulates follicle assembly by targeting Pcna in fetal and neonatal mouse ovaries. Reproduction 2014; 148: 43–54. [DOI:10.1530/REP-13-0508]
16. [16] Unek G, Ozmen A, Kipmen-Korgun D, Korgun ET. Immunolocalization of PCNA, Ki67, p27 and p57 in normal and dexamethasone-induced intrauterine growth restriction placental development in rat. Acta Histochem 2012; 114: 31–40. [DOI:10.1016/j.acthis.2011.02.002]
17. [17] Haggag Y, Nossair M, Elaadli H, Mackey ZB. Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. Alexandria J Vet Sci 2018; 56: 81–87. [DOI:10.5455/ajvs.285213]
18. [18] Jalili T, Khaki A, Ghanbari Z, Imani AM, Hatefi F. A Study of the Therapeutic Effects of Vitamin E on Testicular Tissue Damage Caused by Fluoxetine. Crescent J Med Biol Sci 2014; 1: 37–41.
19. [19] Ruder EH, Hartman TJ, Goldman MB. Impact of oxidative stress on female fertility. Curr Opin Obstet Gynecol 2009; 21: 219–222. [DOI:10.1097/GCO.0b013e32832924ba]
20. [20] Joshi SC, Mathur R, Gajraj A, Sharma T. Influence of methyl parathion on reproductive parameters in male rats. Environ Toxicol Pharmacol 2003; 14: 91–98. [DOI:10.1016/S1382-6689(03)00043-7]
21. [21] Mohseni Kouchsfahani H, parivar K, Tahamtani Y. [Effect of diazinon as a pesticide on oogenesis and ovary structure of balb/c mice strain.] J Sci (Kharazmi University) 2008; 8: 143–152. (in Persian)
22. [22] Oksay T, Nazıroğlu M, Ergün O, Doğan S, Özatik O, Armağan A, et al. N-acetyl cysteine attenuates diazinon exposure-induced oxidative stress in rat testis. Andrologia 2013; 45: 171–177. [DOI:10.1111/j.1439-0272.2012.01329.x]
23. [23] Hiremath MB, Kaliwal BB. Effect of endosulfan on ovarian compensatory hypertrophy in hemicastrated albino mice. Reprod Toxicol 2002; 16: 783–790. [DOI:10.1016/S0890-6238(02)00060-6]
24. [24] Dhondup P, Kaliwal BB. Inhibition of ovarian compensatory hypertrophy by the administration of methyl parathion in hemicastrated albino rats. Reprod Toxicol 1997; 11: 77–84. [DOI:10.1016/S0890-6238(96)00199-2]
25. [25] Amini L, Tehranian N, Movahedin M, Ramezani Tehrani F, Soltanghoraee H. Polycystic ovary morphology (PCOM) in estradiol valerate treated mouse model. Int J Women's Health Reprod Sci 2016; 4: 13–17. [DOI:10.15296/ijwhr.2016.04]
26. [26] Picut CA, Swanson CL, Scully KL, Roseman VC, Parker RF, Remick AK. Ovarian follicle counts using proliferating cell nuclear antigen (PCNA) and semi-automated image analysis in rats. Toxicol Pathol 2008; 36: 674–679. [DOI:10.1177/0192623308317428]
27. [27] Rajabzadeh AA, Bideskan AR, Haghir H, Fazel AR. Morphometrical study of polysialylated neural cell adhesion molecule positive cells in rat pups hippocampus following induction of seizure during pregnancy. Iran Biomed J 2011; 15: 157–163.
28. [28] Yavuz T, Delibas N, Yildirim B, Altuntas I, Candır O, Cora A, et al. Vascular wall damage in rats induced by organophosphorus insecticide methidathion. Toxicol Lett 2005; 155: 59–64. [DOI:10.1016/j.toxlet.2004.08.012]
29. [29] Gomes J, Dawodu AH, Lloyd O, Revitt DM, Anilal SV. Hepatic injury and disturbed amino acid metabolism in mice following prolonged exposure to organophosphorus pesticides. Hum Exp Toxicol 1999; 18: 33–37. [DOI:10.1177/096032719901800105]
30. [30] Mehrpour O, Karrari P, Zamani N, Tsatsakis AM, Abdollahi M. Occupational exposure to pesticides and consequences on male semen and fertility: a review. Toxicol Lett 2014; 230: 146–156. [DOI:10.1016/j.toxlet.2014.01.029]
31. [31] Mattison DR, Thomford PJ. The mechanisms of action of reproductive toxicants. Toxicol Pathol 1989; 17: 364–376. [DOI:10.1177/019262338901700213]
32. [32] Mattiuzzo M, Fiore M, Ricordy R, Degrassi F. Aneuploidy-inducing capacity of two widely used pesticides. Carcinogenesis 2006; 27: 2511–2518. [DOI:10.1093/carcin/bgl102]
33. [33] Carlson K, Ehrich M. Distribution of SH-SY5Y human neuroblastoma cells in the cell cycle following exposure to organophosphorus compounds. J Biochem Mol Toxicol 2008; 22: 187–201. [DOI:10.1002/jbt.20229]
34. [34] Colovic M, Krstic D, Petrovic S, Leskovac A, Joksic G, Savic J, et al. Toxic effects of diazinon and its photodegradation products. Toxicol Lett 2010; 193: 9–18. [DOI:10.1016/j.toxlet.2009.11.022]
35. [35] Penna-Videau S, Buston-Obregon E, Cerme-o-Vivas JR, Chirino D. Malathion affects spermatogenic proliferation in mouse. Int J Morphol 2012; 30: 1399–1407. [DOI:10.4067/S0717-95022012000400023]

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