Volume 16, Issue 9 (September 2018)                   IJRM 2018, 16(9): 549-556 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Golestan Jahromi M, Aflatoonian R, Afsharian P, Aghajanpour S, Shahhoseini M, Aflatoonian A. Altered expression of 3´paralogus HOX A-D clusters in endometriosis disease: A case-control study. IJRM. 2018; 16 (9) :549-556
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1238-en.html
1- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3- Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
4- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , abbas_aflatoonian@yahoo.com
Abstract:   (707 Views)

Background: Endometriosis is a prevalent gynecological disease, with limited known etiology and more researches are required to identify its etiology. In this manner, there is no evidence for expression and function of 3´HOX genes in 4 clusters in the limb and pelvic organs such as the uterus and its disorders (Genes in the HOXA-D clusters are subdivided into 13 paralogous groups).
Objective: This study designed to investigate the expression profile of 5 paralogous (1-5) in four clusters of HOX genes (A, B, C, and D) in ectopic and eutopic tissues of women with endometriosis compared to the normal endometrium.
Materials and Methods: Samples were obtained from thirty patients (15 with and 15 without endometriosis) of reproductive age with normal menstrual cycles. The same patient provided both eutopic and ectopic tissues and control women were laparoscopically checked for the absence of endometriosis. The expression profile of these HOX genes was investigated by quantitative real-time polymerase chain reaction technique.
Results: We observed significant up-regulation of some members of HOXC and D clusters (HOXD1, HOXD3, HOXC4 and HOXC5) in ectopic and eutopic tissues vs. control. Also, our data showed significant down-regulation of all of HOXA and HOXB paralogous except HOXA1 in ectopic tissues versus control.
Conclusion: Our data showed specific cluster dependent modulation of the HOX genes expression in endometriosis (over-expression of some HOX genes in cluster C and D and down-regulation of HOX genes in cluster A and B) in ectopic and eutopic tissues compare to control group. Therefore, it is possible that change of expression level of these genes in endometrium plays a role in the pathogenesis of endometriosis.
Full-Text [PDF 437 kb]   (195 Downloads) |   |   Full-Text (HTML)  (79 Views)  

1. Farquhar CM. Extracts from "clinical evidence": Endometriosis. BMJ 2000; 320: 1449-1452. [DOI:10.1136/bmj.320.7247.1449]
2. Houston DE. Evidence for the risk of pelvic endometriosis by age, race and socioeconomic status. Epidemiol Rev 1984; 6: 167-191. [DOI:10.1093/oxfordjournals.epirev.a036270]
3. Borghese B, Mondon , o l C, ayt , ignot T , Vaiman D, et al. Gene expression profile for ectopic versus eutopic endometrium provides new insights into endometriosis oncogenic potential. Mol Endocrinol 2008; 22: 2557-2562. [DOI:10.1210/me.2008-0322]
4. Mahdian S, Aflatoonian R, Yazdi RS, Yaghmaei P, Ramazanali F, Afsharian P, et al. Macrophage migration inhibitory factor as a potential biomarker of endometriosis. Fertil Steril 2015; 103: 153-159. [DOI:10.1016/j.fertnstert.2014.09.031]
5. Bischoff FZ, Simpson JL. Heritability and molecular genetic studies of endometriosis. Hum Reprod Update 2000; 6: 37-44. [DOI:10.1093/humupd/6.1.37]
6. Šmuc T, Pucelj R, Šinkovec , Husen B, Thole H, Lanisnik Rižner TL. Expression analysis of the genes involved in estradiol and progesterone action in human ovarian endometriosis. Gynecol Endocrinol 2007; 23: 105-111. [DOI:10.1080/09513590601152219]
7. Tsudo T, Harada T, Iwabe T, Tanikawa M, Nagano Y, Ito M, et al. Altered gene expression and secretion of interleukin-6 in stromal cells derived from endometriotic tissues. Fertil Steril 2000; 73: 205-211. [DOI:10.1016/S0015-0282(99)00496-3]
8. Treloar SA, Wicks J, Nyholt DR, Montgomery GW, Bahlo M, Smith V, et al. Genomewide linkage study in 1,176 affected sister pair families identifies a significant susceptibility locus for endometriosis on chromosome 10q26. Am J Hum Genet 2005; 77: 365-376. [DOI:10.1086/432960]
9. Graham A, Papalopulu N, Krumlauf R. The murine and Drosophila homeobox gene complexes have common features of organization and expression. Cell 1989; 57: 367-378. [DOI:10.1016/0092-8674(89)90912-4]
10. Krumlauf R. Evolution of the vertebrate Hox homeobox genes. Bioessays 1992; 14: 245-252. [DOI:10.1002/bies.950140408]
11. Zeltser L, Desplan C, Heintz N. Hoxb-13: a new Hox gene in a distant region of the HOXB cluster maintains colinearity. Development 1996; 122: 2475-2484.
12. Duboule D. The vertebrate limb: a model system to study the Hox/HOM gene network during development and evolution. Bioessays 1992; 14: 375-384. [DOI:10.1002/bies.950140606]
13. Cillo C. HOX genes in human cancers. Invasion metastasis 1994; 14: 38-49.
14. Daftary GS, Taylor HS. Implantation in the human: the role of HOX genes. Semin Reprod Med 2000; 18: 311-320. [DOI:10.1055/s-2000-12568]
15. Taylor HS. The role of HOX genes in the development and function of the female reproductive tract. Semin Reprod Med 2000; 18: 81-89. [DOI:10.1055/s-2000-13478]
16. Akbas GE, Taylor HS. HOXC and HOXD gene expression in human endometrium: lack of redundancy with HOXA paralogs. Biol Reprod 2004; 70: 39-45. [DOI:10.1095/biolreprod.102.014969]
17. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408. [DOI:10.1006/meth.2001.1262]
18. Baldi A, Campioni M, Signorile PG. Endometriosis: pathogenesis, diagnosis, therapy and association with cancer. Oncol Rep 2008; 19: 843-846. [DOI:10.3892/or.19.4.843]
19. Burney RO, Giudice LC. Pathogenesis and pathophysiology of endometriosis. Fertil Steril 2012; 98: 511-519. [DOI:10.1016/j.fertnstert.2012.06.029]
20. Philippidou P, Dasen JS. Hox genes: choreographers in neural development, architects of circuit organization. Neuron 2013; 80: 12-34. [DOI:10.1016/j.neuron.2013.09.020]
21. Dasen JS, Tice BC, Brenner-Morton S, Jessell TM. A Hox regulatory network establishes motor neuron pool identity and target-muscle connectivity. Cell 2005; 123: 477-491. [DOI:10.1016/j.cell.2005.09.009]
22. McGinnis W, Krumlauf R. Homeobox genes and axial patterning. Cell 1992; 68: 283-302. [DOI:10.1016/0092-8674(92)90471-N]
23. Mark M, Rijli FM, Chambon P. Homeobox genes in embryogenesis and pathogenesis. Pediatr Res 1997; 42: 421-429. [DOI:10.1203/00006450-199710000-00001]
24. Taylor HS, Bagot C, Kardana A, Olive D, Arici A. HOX gene expression is altered in the endometrium of women with endometriosis. Hum Reprod 1999; 14: 1328-1331. [DOI:10.1093/humrep/14.5.1328]
25. Zanatta A, Rocha AM, Carvalho FM, Pereira RM, Taylor HS, Motta EL, et al. The role of the Hoxa10/HOXA10 gene in the etiology of endometriosis and its related infertility: a review. J Assist Reprod Genet 2010; 27: 701-710. [DOI:10.1007/s10815-010-9471-y]
26. Matsuzaki S, Canis M, Darcha C, Pouly JL, Mage G. HOXA-10 expression in the mid-secretory endometrium of infertile patients with either endometriosis, uterine fibromas or unexplained infertility. Hum Reprod 2009; 24: 3180-3187. [DOI:10.1093/humrep/dep306]
27. Szczepańska, Wirstlein P, Skrzypczak, agodziński PP. Expression of HOXA11 in the mid-luteal endometrium from women with endometriosis-associated infertility. Reprod Biol Endocrinol 2012; 10: 1-8. [DOI:10.1186/1477-7827-10-1]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb