Volume 6, Issue 4 (7-2008)                   IJRM 2008, 6(4): 125-0 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Peyghambari F, Salehnia M, Forouzandeh Moghadam M, Rezazadeh Valujerdi M, Hajizadeh E. The changes in morphology and morphometrical indices of endometrium of ovariectomized mice in response to exogenous ovarian hormones. IJRM. 2008; 6 (4) :125-0
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-120-en.html
1- Department of Anatomy, Tarbiat Modares University, Iran
2- Department of Anatomy, Tarbiat Modares University, Iran , mogdeh@dr.com
3- Department of Biotechnology, Tarbiat Modares University, Iran
4- Department of Biostatistics, Tarbiat Modares University, Iran
Abstract:   (504 Views)
Background: The preparation of endometrium for embryo reception and implantation are controlled by ovarian hormones. These hormones have distinct cyclical changes during estrus cycle.
Objective: The aim of this study was to evaluate the changes in morphology and morphometrical indices of endometrium by daily injections of estrogen and progesterone in ovariectomized mouse.
Materials and Methods: In total 60 adult NMRI female mice were ovariectomized and after two weeks, they were randomly divided into five groups: control, sham group, estrogen treated mice (which received daily dosage of 0.5 ml/mouse of hormone for five days), progesterone treated mice (which received daily dosage of 0.2 ml/mouse of progesterone hormone for five days) and estrogen-progesterone treated mice (they received 0.5 ml/mouse estrogen on the first day and 0.2 ml/mouse progesterone injections from the second day to the fifth day of treatment). The mice were sacrificed in every day (n=5) up to five days after treatment and their uterine horns were obtained and processed for morphological and morphometrical studies.
Results: On the second day of treatment, the diameter of glands was observed to be more in the progesterone group (53.75±6.32μ) than this in the estrogen (45.13±7.78 μ) and estrogen-progesterone treated groups (48.17±13.58 μ). While, the number of glands (76.25±17.37) and thickness of endometrium (39.58±3.37 μ) were observed to be more in the estrogen treated group (p=0.01).
Conclusion: Progesterone had effect on the gland whereas estrogen caused increased in height of surface epithelium of endometrium. Overall, the day 2 after treatment (in all experimental groups) is suitable day for sampling for further studies.
Full-Text [PDF 203 kb]   (55 Downloads) |   |   Full-Text (HTML)  (22 Views)  
Type of Study: Original Article |

References
1. Lessy BA. Two pathways of progesterone action in the human endometrium: implications for implantation and contraception. Steroids 2003; 68: 809-815. [DOI:10.1016/j.steroids.2003.09.004]
2. Song H, Han K, Lim H. Progesterone supplementation extends uterine receptivity for blastocyst implantation in mice. Reproduction 2007; 133: 487-493. [DOI:10.1530/REP-06-0330]
3. Ma WG, Song H, Das SK, Paria BC, Dey SK. Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation. Proc Natl Acad Sci USA 2003; 100: 2963-2968. [DOI:10.1073/pnas.0530162100]
4. Dey SK. Implantation. In: Adashi EY, Rock JA,Rosenwaks Z, eds. Reproductive endocrinology, surgery and technology. 1996 New York: Lippincott-Raven; 421-434.
5. Das SK, Chakraborty I, Paria BC, Wang XN, Plowman G, Dey SK. Amphiregulin is an implantation-specific and progesterone-regulated gene in the mouse uterus. Mol Endocrinol 1995; 9: 691-705. [DOI:10.1210/mend.9.6.8592515]
6. Horne FM, Blithe DL. Progesterone receptor modulators and the endometrium: changes and consequences. Hum Reprod Update 2007; 13: 567-580. [DOI:10.1093/humupd/dmm023]
7. Johnson ML, Redmer DA, Reynolds LP. Effects of ovarian steroids on uterine growth, morphology and cell proliferation in ovariectomized, steroid-treated ewes. Biol Reprod 1997; 57:588-596. [DOI:10.1095/biolreprod57.3.588]
8. Cheon YP, Xu X, Bagchi MK, Bagchi IC. Immune-responsive gene 1 is a novel target of progesterone receptor and plays a critical role during implantation in the mouse. Endocrinol 2003; 144:5623-5630. [DOI:10.1210/en.2003-0585]
9. Hewitt SC, Korach KS. Progesterone action and responses in the alphaERKO mouse. Steroids 2000; 65: 551-557. [DOI:10.1016/S0039-128X(00)00113-6]
10. Walter LM, Rogers PA, Girling JE. The role of progesterone in endometrial angiogenesis in pregnant and ovariectomised mice. Reproduction 2005; 129:765-777. [DOI:10.1530/rep.1.00625]
11. Sengupta J, Ghosh D. Role of progesterone on peri-implantation stage endometium-emberyo interaction in the primate. Steroids 2000; 65:753-762. [DOI:10.1016/S0039-128X(00)00191-4]
12. Brinsfield TH, Hawk HW. Ultrastructure of sheep endometrial. stromal cells after ovariectomy and hormone treatment Biol Reprod 1974;10:98-102. [DOI:10.1095/biolreprod10.1.98]
13. Williams T, Rogers AW. Morphometric studies of the luminal epithelium in the rat uterus to exogenous hormones. Anat 1980; 130: 867-881.
14. Murray MK. The effect of estrogen and progesterone on structural changes in the uterine glandular epithelium of the ovariectomized sheep. Biol Reprod 1992; 47:408-417. [DOI:10.1095/biolreprod47.3.408]
15. Heryanto B, Rogers PA. Regulation of endometrial cell proliferation by oestrogen and progesterone in the ovariectomized mouse. Reproduction 2002; 123:107-113. [DOI:10.1530/rep.0.1230107]
16. Martin L, Pollard JW, Fagg B. Oestriol, oestradiol- 17beta and the proliferation and death of uterine cells. Endocrinol 1976; 69:103-115. [DOI:10.1677/joe.0.0690103]
17. Miller BG. Delayed interactions between progesterone and low dose of 17beta-estradiol in the mouse uterus. Endocrinol 1979; 104:26-33. [DOI:10.1210/endo-104-1-26]
18. Stein B, Kramer B. The effect of exogenous gonadotropic hormones on the endometrium of the rat. Anat 1989; 164:123-130.
19. Huet-Hudson YM, Andrews GK, Dey SK. Cell type-specific localization of c-myc protein in the mouse uterus: modulation by steroid hormones and analysis of the periimplantation period. Endocrinol 1989; 125:1683-1690. [DOI:10.1210/endo-125-3-1683]
20. Finn CA, Porter DG . The uterus. In: Reproductive biology handbooks. Acton, MA: Publishing Sciences Group 1975; 1:42-56.
21. Pollard RM,Finn CA. The effect of ovariectomy of puberty on cell proliferation and differentiation in the endometrium of the aged mouse. Biol Reprod 1974; 10:74-77. [DOI:10.1095/biolreprod10.1.74]
22. Zhang Q, Paria BC. Importance of uterine cell death, renewal, and their hormonal regulation in hamsters that show progesterone-dependent implantation. Endocrinol 2006; 147:2215-2227. [DOI:10.1210/en.2005-1555]
23. Chu PY, Lee CS, Moore PF, Wright PJ. Oestrogen and progesterone treated ovariectomized bitches: a model for the study of uterine function. Reprod Fertil 2001; 57:45-54.
24. Evans GS, Gibson DF, Roberts SA, Hind TM, Potten CS. Proliferative changes in the genital tissue of female mice during the oestrous cycle. Cell Tissue Kinet 1990; 23:619-35. [DOI:10.1111/j.1365-2184.1990.tb01350.x]
25. Martin L, Finn CA, Trinder G. Hypertrophy and hyperplasia in the mouse uterus after oestrogen treatment: an autoradiographic study. J Endocrinol 1973; 56:133-144. [DOI:10.1677/joe.0.0560133]
26. Finn CA, Martin L. The role of the oestrogen secreted before oestrus in the preparation of the uterus for implantation in the mouse. J Endocrinol 1970; 47:431-438. [DOI:10.1677/joe.0.0470431]
27. Tong W, Pollard JW. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biol 1999; 19:2251-2264. [DOI:10.1128/MCB.19.3.2251]
28. Terada N, Yamamoto R, Takada T, Miyake T, Terakawa N, Wakimoto H, Taniguchi H, et al. Inhibitory effects of progesterone on cell death of mouse uterine epithelium . J Steroid Biochem 1989; 33:1091-1096. [DOI:10.1016/0022-4731(89)90414-7]
29. Girling JE, Lederman FL, Walter LM, Rogers PA. Progesterone, but not estrogen, stimulates vessel maturation in the mouse endometrium. Endocrinol 2007; 148:5433-5441. [DOI:10.1210/en.2007-0856]

Send email to the article author


© 2020 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb