Volume 16, Issue 8 (August 2018)                   IJRM 2018, 16(8): 519-528 | Back to browse issues page

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Fani M, Mohammadipour A, Ebrahimzadeh Bideskan A. The effect of crocin on testicular tissue and sperm parameters of mice offspring from mothers exposed to atrazine during pregnancy and lactation periods: An experimental study. IJRM. 2018; 16 (8) :519-528
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1178-en.html
1- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2- 1. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , EbrahimzadehBA@mums.ac.ir
Abstract:   (255 Views)
Background: Atrazine as a herbicide may affect the human’s health. Crocin may protect atrazine-induced damages.
Objective: The aim of this study was to evaluate the effects of atrazine on mice testicular tissue and sperm parameters and protective effects of Crocin on probably atrazine-induced damages.
Materials and Methods: in this experimental study, 24 pregnant Balb/c mice were randomly divided to 4 groups: I: Atrazine (10 mg/kg), II: Atrazine-Crocin, III: Crocin (10 mg/kg) and IV: Normal saline. Administrations were done daily by gavage during pregnancy and lactation. In the end, two male offspring were randomly selected from every mother and sacrificed respectively on 23 and 75 postnatal days. Then, their epididymides were removed for sperm parameters investigation and their testes were prepared to evaluate apoptosis by means of TUNEL technique.
Results: The mean number of sperms in the atrazine group was lower compared to other groups and increased in the atrazine-crocin group compared with atrazine group significantly (p=0.001). Sperm abnormality was increased in the atrazine group compared with the normal saline group and decreased in the atrazine-crocin group compared with atrazine group significantly (p≤0.001). TUNEL-positive spermatogonia in 23 days old offspring increased significantly in the atrazine group compared with other groups (p=0.03). TUNEL-positive spermatogenic cells in 75 days old offspring was significantly increased in the atrazine group compared with the saline group (p≤0.001).
Conclusion: Atrazine exposure may lead to decrease the number of sperms, increase sperms abnormality, spermatogenic cell apoptosis and height of germinal epithelium. These complications may improve by crocin administration.
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Type of Study: Original Article |
Received: 2018/08/27 | Accepted: 2018/08/27 | Published: 2018/08/27

1. Pavlovic D, Vrbnicanin S, Bozic D, Fischer AJ. Morphophysiological traits and atrazine sensitivity in Chenopodium album L. Pest Manag Sci 2008; 64: 101-107. [DOI:10.1002/ps.1473]
2. Farombi EO, Abarikwu SO, Adesiyan AC, Oyejola TO. Quercetin exacerbates the effects of subacute treatment of atrazine on reproductive tissue antioxidant defence system, lipid peroxidation and sperm quality in rats. Andrologia 2013; 45: 256-265. [DOI:10.1111/and.12001]
3. Banks KE, Hunter DH, Wachal DJ. Chlorpyrifos in surface waters before and after a federally mandated ban. Environ Int 2005; 31: 351-356. [DOI:10.1016/j.envint.2004.08.007]
4. Gojmerac T, Ostojić Z, Pauković D, Pleadin J, Ţurić M. Evaluation of surface water pollution with atrazine, an endocrine disruptor chemical, in agricultural areas of Turopolje, Croatia. Bull Environ Contam Toxicol 2006; 76: 490-496. [DOI:10.1007/s00128-006-0947-5]
5. McConnell LL, Nelson E, Rice CP, Baker JE, Johnson WE, Harman JA, et al. Chlorpyrifos in the air and surface water of Chesapeake Bay: predictions of atmospheric deposition fluxes. Environ Sci Technol 1997; 31: 1390-1398. [DOI:10.1021/es960614x]
6. Liu W, Du Y, Liu J, Wang H, Sun D, Liang D, et al. Effects of atrazine on the oxidative damage of kidney in Wister rats. Int J Clin Exp Med 2014; 7: 3235-3243.
7. Cooper RL, Stoker TE, Tyrey L, Goldman JM, McElroy WK. Atrazine disrupts the hypothalamic control of pituitary-ovarian function. Toxicol Sci 2000; 53: 297-307. [DOI:10.1093/toxsci/53.2.297]
8. Song Y, Jia ZC, Chen JY, Hu JX, Zhang LS. Toxic effects of atrazine on reproductive system of male rats. Biomed Environ Sci 2014; 27: 281-288.
9. Rosenberg BG, Chen H, Folmer J, Liu J, Papadopoulos V, Zirkin BR. Gestational exposure to atrazine: effects on the postnatal development of male offspring. J Androl 2008; 29: 304-311. [DOI:10.2164/jandrol.107.003020]
10. Kniewald J, Jakominić M, Tomljenović A, Šimić B, Romac P, Vranešić Ð, et al. Disorders of male rat reproductive tract under the influence of atrazine. J Appl Toxicol 2000; 20: 61-68. https://doi.org/10.1002/(SICI)1099-1263(200001/02)20:1<61::AID-JAT628>3.0.CO;2-3 [DOI:10.1002/(SICI)1099-1263(200001/02)20:13.0.CO;2-3]
11. Trentacoste SV, Friedmann AS, Youker RT, Breckenridge CB, Zirkin BR. Atrazine effects on testosterone levels and androgen‐dependent reproductive organs in peripubertal male rats. J Androl 2001; 22: 142-148.
12. Lin Z, Dodd CA, Xiao S, Krishna S, Ye X, Filipov NM. Gestational and lactational exposure to atrazine via the drinking water causes specific behavioral deficits and selectively alters monoaminergic systems in C57BL/6 mouse dams, juvenile and adult offspring. Toxicol Sci 2014; 141: 90-102. [DOI:10.1093/toxsci/kfu107]
13. Chandak N, Bhardwaj JK, Zheleva-Dimitrova D, Kitanov G, Sharma RK, Sharma PK, et al. Effective attenuation of atrazine-induced histopathological changes in testicular tissue by antioxidant N-phenyl-4-aryl-polyhydroquinolines. J Enzyme Inhib Med Chem 2015; 30: 722-729. [DOI:10.3109/14756366.2014.960864]
14. Jin Y, Zhang X, Shu L, Chen L, Sun L, Qian H, et al. Oxidative stress response and gene expression with atrazine exposure in adult female zebrafish (Danio rerio). Chemosphere 2010; 78: 846-852. [DOI:10.1016/j.chemosphere.2009.11.044]
15. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002; 29: 817-827. [DOI:10.1016/S0094-0143(02)00081-2]
16. Rahimipour M, Talebi AR, Anvari M, Abbasi Sarcheshmeh A, Omidi M. Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice. Iran J Reprod Med 2014; 12: 307-312.
17. Aung HH, Wang CZ, Ni M, Fishbein A, Mehendale SR, Xie JT, et al. Crocin from Crocus sativus possesses significant anti-proliferation effects on human colorectal cancer cells. Exp Oncol 2007; 29: 175-180.
18. Bolhassani A, Khavari A, Bathaie SZ. Saffron and natural carotenoids: Biochemical activities and anti-tumor effects. Biochim Biophys Acta 2014; 1845: 20-30.
19. Wang Y, Wang Q, Yu W, Du H. Crocin Attenuates Oxidative Stress and Myocardial Infarction Injury in Rats. Int Heart J 2018: 59: 387-393. [DOI:10.1536/ihj.17-114]
20. Al-Snafi AE. The pharmacology of Crocus sativus- A review. IOSR J Pharm 2016; 6: 8-38.
21. Sarshoori JR, Asadi MH, Mohammadi MT. Neuroprotective effects of crocin on the histopathological alterations following brain ischemia-reperfusion injury in rat. Iran J Basic Med Sci 2014; 17: 895-902.
22. Rosenberg BG, Chen H, Folmer J, Liu J, Papadopoulos V, Zirkin BR. Gestational exposure to atrazine: effects on the postnatal development of male offspring. J Androl 2008; 29: 304-311. [DOI:10.2164/jandrol.107.003020]
23. Goyal SN, Arora S, Sharma AK, Joshi S, Ray R, Bhatia J, et al. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine 2010; 17: 227-232. [DOI:10.1016/j.phymed.2009.08.009]
24. Bakhtiary Z, Shahrooz R, Ahmadi A, Zarei L. Evaluation of antioxidant effects of crocin on sperm quality in cyclophosphamide treated adult mice. Vet Res Forum 2014; 5: 213-218.
25. Khordad E, Fazel A, Ebrahimzadeh Bideskan A. The effect of ascorbic acid and garlic administration on lead-induced apoptosis in rat offspring's eye retina. Iran Biomed J 2013; 17: 206-213.
26. Ataei ML, Ebrahimzadeh-bideskan AR. The effects of nano-silver and garlic administration during pregnancy on neuron apoptosis in rat offspring hippocampus. Iran J Basic Med Sci 2014; 17: 411-418.
27. Bagheri-Abassi F, Alavi H, Mohammadipour A, Motejaded F, Ebrahimzadeh-Bideskan A. The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus. Iran J Basic Med Sci 2015; 18: 644-648.
28. Bandegi AR, Rashidy-Pour A, Vafaei AA, Ghadrdoost B. Protective effects of Crocus sativus L. extract and crocin against chronic-stress induced oxidative damage of brain, liver and kidneys in rats. Adv Pharm Bull 2014; 4 (Suppl.): 493-499.
29. Gely-Pernot A, Hao C, Becker E, Stuparevic I, Kervarrec C, Chalmel F, et al. The epigenetic processes of meiosis in male mice are broadly affected by the widely used herbicide atrazine. BMC Genomics 2015; 16: 885-891. [DOI:10.1186/s12864-015-2095-y]
30. Victor-Costa AB, Bandeira SM, Oliveira AG, Mahecha GA, Oliveira CA. Changes in testicular morphology and steroidogenesis in adult rats exposed to Atrazine. Reprod Toxicol 2010; 29: 323-331. [DOI:10.1016/j.reprotox.2009.12.006]
31. Salahshoor MR, Khazaei M, Jalili C, Keivan M. Crocin improves damage induced by nicotine on a number of reproductive parameters in male mice. Int J Fertil Steril 2016; 10: 71-78.
32. Song Y, Jia ZC, Chen JY, Hu JX, Zhang LS. Toxic effects of atrazine on reproductive system of male rats. Biomed Environ Sci 2014; 27: 281-288.
33. Najafi G, Hobe Naghi R, Hoshyari A, Moghadaszadeh M, Ghorbanzadeh B. The effect of atrazine on spermic parameters and fertility potential in mature rats. Arak Med Uni J 2013; 15: 85-94.
34. Bratton SB, MacFarlane M, Cain K, Cohen GM. Protein complexes activate distinct caspase cascades in death receptor and stress-induced apoptosis. Exp Cell Res 2000; 256: 27-33. [DOI:10.1006/excr.2000.4835]
35. Rayner JL, Wood C, Fenton SE. Exposure parameters necessary for delayed puberty and mammary gland development in Long–Evans rats exposed in utero to atrazine. Toxicol Appl pharmacol 2004; 195: 23-34. [DOI:10.1016/j.taap.2003.11.005]
36. Pogrmic-Majkic K, Kaisarevic S, Fa S, Dakic V, Glisic B, Hrubik J, et al. Atrazine effects on antioxidant status and xenobiotic metabolizing enzymes after oral administration in peripubertal male rat. Environ Toxicol Pharmacol 2012; 34: 495-501. [DOI:10.1016/j.etap.2012.06.004]
37. Nazem H, Modaresi M, Messripour M, Marghmaleki MA, Ebadi AG. Effect of saffron extract on pituitary-testis axis in mice. Asian J Chem 2009; 21: 1616-1618.

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